Mutagenetix > Incidental Mutation

Incidental Mutation 'R1164:Cks1b'

100780

Institutional Source

Beutler Lab

Gene Symbol

Cks1b

Ensembl Gene

ENSMUSG00000028044

Gene Name

CDC28 protein kinase 1b

Synonyms

2610005D03Rik, Cks1, 2410005G18Rik

MMRRC Submission

039237-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R1164 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89322779-89325598 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

C to A at 89323249 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000140336 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029679] [ENSMUST00000039110] [ENSMUST00000050398] [ENSMUST00000107417] [ENSMUST00000107422] [ENSMUST00000107426] [ENSMUST00000107429] [ENSMUST00000129308] [ENSMUST00000154791] [ENSMUST00000162701] [ENSMUST00000191485] [ENSMUST00000125036] [ENSMUST00000128238] [ENSMUST00000183484]

AlphaFold

P61025

Predicted Effect

unknown
Transcript: ENSMUST00000029679
AA Change: R70L

SMART Domains

Protein: ENSMUSP00000029679
Gene: ENSMUSG00000028044
AA Change: R70L

Domain	Start	End	E-Value	Type
CKS	5	74	4.1e-48	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000039110

SMART Domains

Protein: ENSMUSP00000035361
Gene: ENSMUSG00000042626

Domain	Start	End	E-Value	Type
low complexity region	6	17	N/A	INTRINSIC
PTB	47	211	2.15e-31	SMART
SH2	372	451	1.71e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000050398

SMART Domains

Protein: ENSMUSP00000051366
Gene: ENSMUSG00000042642

Domain	Start	End	E-Value	Type
MoCF_biosynth	19	180	7.52e-24	SMART
Pfam:PAPS_reduct	303	460	5.2e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107417

SMART Domains

Protein: ENSMUSP00000103040
Gene: ENSMUSG00000042626

Domain	Start	End	E-Value	Type
PTB	2	166	2.15e-31	SMART
SH2	327	406	1.71e-26	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000107422
AA Change: R48L

SMART Domains

Protein: ENSMUSP00000103045
Gene: ENSMUSG00000028044
AA Change: R48L

Domain	Start	End	E-Value	Type
CKS	1	52	3.88e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107426

SMART Domains

Protein: ENSMUSP00000103049
Gene: ENSMUSG00000042642

Domain	Start	End	E-Value	Type
MoCF_biosynth	19	180	7.52e-24	SMART
Pfam:PAPS_reduct	303	460	4.7e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107429

SMART Domains

Protein: ENSMUSP00000103052
Gene: ENSMUSG00000042642

Domain	Start	End	E-Value	Type
MoCF_biosynth	19	174	2.06e-21	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153334

Predicted Effect

probably benign
Transcript: ENSMUST00000129308

SMART Domains

Protein: ENSMUSP00000122252
Gene: ENSMUSG00000042642

Domain	Start	End	E-Value	Type
MoCF_biosynth	19	180	7.52e-24	SMART
Pfam:PAPS_reduct	303	460	4.7e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000154791

SMART Domains

Protein: ENSMUSP00000123635
Gene: ENSMUSG00000042626

Domain	Start	End	E-Value	Type
low complexity region	6	17	N/A	INTRINSIC
Pfam:PID	52	100	5.7e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000162701

SMART Domains

Protein: ENSMUSP00000125654
Gene: ENSMUSG00000042642

Domain	Start	End	E-Value	Type
MoCF_biosynth	19	99	1.11e0	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000191485

SMART Domains

Protein: ENSMUSP00000140336
Gene: ENSMUSG00000042626

Domain	Start	End	E-Value	Type
low complexity region	6	17	N/A	INTRINSIC
PTB	47	211	2.15e-31	SMART
SH2	372	451	1.71e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125036

SMART Domains

Protein: ENSMUSP00000115509
Gene: ENSMUSG00000042626

Domain	Start	End	E-Value	Type
PTB	1	155	1.5e-21	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000128238

SMART Domains

Protein: ENSMUSP00000119293
Gene: ENSMUSG00000042626

Domain	Start	End	E-Value	Type
low complexity region	6	17	N/A	INTRINSIC
Pfam:PID	52	144	7.7e-19	PFAM
Pfam:PID	134	190	7.8e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000183484

SMART Domains

Protein: ENSMUSP00000138900
Gene: ENSMUSG00000028044

Domain	Start	End	E-Value	Type
Pfam:CKS	5	36	2.4e-8	PFAM

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 96.0%
20x: 91.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. [provided by RefSeq, Sep 2008]
PHENOTYPE: Embryos homozygous for a knock-out allele exhibit a reduced body size that persists through adulthood while heterozygous mice are intermediate in size. Mutant mouse embryonic fibroblasts (MEFs) proliferate poorly and senesce earlier than wild-type MEFs. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca3	T	A	17: 24,621,305 (GRCm39)	M1055K	probably damaging	Het
Adprh	A	T	16: 38,270,702 (GRCm39)	D34E	probably benign	Het
Aldh1a1	A	T	19: 20,595,310 (GRCm39)	M80L	probably benign	Het
Arap2	T	A	5: 62,840,820 (GRCm39)	D682V	probably damaging	Het
Atp4a	T	A	7: 30,417,117 (GRCm39)	L500Q	probably benign	Het
Atp6v1c2	T	C	12: 17,358,317 (GRCm39)	E10G	probably damaging	Het
B4galt2	G	A	4: 117,734,141 (GRCm39)	R299W	possibly damaging	Het
Bltp3a	T	C	17: 28,114,354 (GRCm39)		probably null	Het
Brinp1	A	C	4: 68,716,928 (GRCm39)	S307A	probably benign	Het
Cacna2d1	G	A	5: 16,566,874 (GRCm39)		probably null	Het
Ccdc3	T	C	2: 5,146,077 (GRCm39)	V137A	possibly damaging	Het
Ccnb1ip1	T	C	14: 51,029,594 (GRCm39)	K156R	possibly damaging	Het
Cfap77	A	T	2: 28,852,700 (GRCm39)	W191R	probably damaging	Het
Chga	A	G	12: 102,529,304 (GRCm39)	E427G	probably damaging	Het
Chrnd	A	T	1: 87,120,267 (GRCm39)	Y32F	probably benign	Het
Creld2	G	A	15: 88,704,834 (GRCm39)	W103*	probably null	Het
Dip2a	C	A	10: 76,112,231 (GRCm39)	R1098L	possibly damaging	Het
Dmbx1	T	A	4: 115,775,455 (GRCm39)	H275L	probably damaging	Het
Dmrt2	A	G	19: 25,655,357 (GRCm39)	M319V	possibly damaging	Het
Dock8	A	G	19: 25,067,391 (GRCm39)	Y345C	probably benign	Het
Dpp6	A	G	5: 27,926,103 (GRCm39)	T668A	probably benign	Het
Eef1d	A	G	15: 75,774,526 (GRCm39)		probably null	Het
Epb41l3	A	G	17: 69,581,762 (GRCm39)	T568A	possibly damaging	Het
Erc2	A	G	14: 28,024,929 (GRCm39)	R603G	probably damaging	Het
Fam83d	T	C	2: 158,625,170 (GRCm39)	S254P	probably damaging	Het
Fcgr4	A	T	1: 170,856,739 (GRCm39)	H202L	possibly damaging	Het
Gm4922	C	T	10: 18,659,469 (GRCm39)	A418T	possibly damaging	Het
Kmo	A	G	1: 175,486,125 (GRCm39)	H416R	probably benign	Het
Lao1	A	G	4: 118,822,602 (GRCm39)	N174S	probably benign	Het
Lrwd1	A	T	5: 136,159,844 (GRCm39)	H406Q	probably benign	Het
Magoh	A	G	4: 107,744,459 (GRCm39)	I143V	probably benign	Het
Mpz	A	G	1: 170,986,008 (GRCm39)	H49R	possibly damaging	Het
Nav1	T	C	1: 135,400,148 (GRCm39)	N474S	probably benign	Het
Ndufb10	T	G	17: 24,941,757 (GRCm39)	E68D	probably benign	Het
Obscn	T	C	11: 58,926,913 (GRCm39)	D5534G	possibly damaging	Het
Or13c7d	T	C	4: 43,770,991 (GRCm39)	T7A	probably benign	Het
Or14j5	T	A	17: 38,161,575 (GRCm39)	F31I	probably damaging	Het
Or1j16	A	T	2: 36,530,132 (GRCm39)	Y27F	probably benign	Het
Or4d10	A	T	19: 12,051,605 (GRCm39)	Y130*	probably null	Het
Or52l1	A	T	7: 104,830,040 (GRCm39)	F160Y	probably benign	Het
Or8k30	C	A	2: 86,339,028 (GRCm39)	T75K	probably damaging	Het
Padi1	A	T	4: 140,559,640 (GRCm39)	V79E	possibly damaging	Het
Pdha2	A	G	3: 140,917,260 (GRCm39)	Y83H	probably damaging	Het
Phpt1	A	G	2: 25,464,727 (GRCm39)	I42T	probably damaging	Het
Pot1b	A	C	17: 55,981,085 (GRCm39)	S310A	probably benign	Het
Ptpn13	G	A	5: 103,637,639 (GRCm39)	V176I	probably damaging	Het
Ptprf	A	G	4: 118,114,689 (GRCm39)	S189P	probably damaging	Het
Rere	A	T	4: 150,619,341 (GRCm39)	Q381L	unknown	Het
Rfwd3	C	T	8: 112,014,874 (GRCm39)	R326Q	probably damaging	Het
Scn8a	G	A	15: 100,938,043 (GRCm39)	C1804Y	probably benign	Het
Sema3c	G	A	5: 17,883,312 (GRCm39)	D307N	probably benign	Het
Siah2	T	C	3: 58,583,737 (GRCm39)	E183G	probably benign	Het
Smarca5	G	A	8: 81,437,260 (GRCm39)	L699F	probably damaging	Het
Smo	T	A	6: 29,754,718 (GRCm39)	S263T	probably benign	Het
Sox2	A	G	3: 34,704,848 (GRCm39)	E95G	probably damaging	Het
T	T	C	17: 8,658,771 (GRCm39)	S171P	probably benign	Het
Tmc7	G	T	7: 118,141,247 (GRCm39)	A628D	probably benign	Het
Tmem45a2	A	G	16: 56,869,789 (GRCm39)	S52P	probably damaging	Het
Tubb3	C	T	8: 124,148,186 (GRCm39)	A373V	probably damaging	Het
Upp2	A	G	2: 58,653,716 (GRCm39)	Y69C	probably damaging	Het
Utp4	A	G	8: 107,627,476 (GRCm39)		probably null	Het
Vmn1r59	T	A	7: 5,457,410 (GRCm39)	M117L	probably benign	Het
Xkr8	T	C	4: 132,459,722 (GRCm39)	S19G	probably benign	Het
Zbed6	G	A	1: 133,586,941 (GRCm39)	T132I	probably damaging	Het
Zbtb24	T	C	10: 41,340,523 (GRCm39)	Y518H	probably damaging	Het
Zfp324	T	C	7: 12,705,551 (GRCm39)	I580T	probably benign	Het
Zfp995	G	A	17: 22,098,960 (GRCm39)	H425Y	probably damaging	Het

Other mutations in Cks1b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
PIT4486001:Cks1b	UTSW	3	89,323,621 (GRCm39)	missense	probably damaging	1.00
R5378:Cks1b	UTSW	3	89,323,608 (GRCm39)	missense	probably damaging	1.00
R7719:Cks1b	UTSW	3	89,323,635 (GRCm39)	missense	probably benign	0.01

Predicted Primers

Posted On

2014-01-15