|Institutional Source||Beutler Lab|
|Gene Name||dedicator of cyto-kinesis 3|
|Is this an essential gene?||Possibly non essential (E-score: 0.483)|
|Stock #||R1184 (G1)|
|Chromosomal Location||106892825-107231909 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 106969800 bp|
|Amino Acid Change||Serine to Proline at position 877 (S877P)|
|Ref Sequence||ENSEMBL: ENSMUSP00000047652 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000044532]|
|Predicted Effect||probably damaging
AA Change: S877P
PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
AA Change: S877P
|Predicted Effect||probably benign
|Meta Mutation Damage Score||0.082|
|Coding Region Coverage||
|Validation Efficiency||100% (83/83)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is specifically expressed in the central nervous system (CNS). It encodes a member of the DOCK (dedicator of cytokinesis) family of guanine nucleotide exchange factors (GEFs). This protein, dedicator of cytokinesis 3 (DOCK3), is also known as modifier of cell adhesion (MOCA) and presenilin-binding protein (PBP). The DOCK3 and DOCK1, -2 and -4 share several conserved amino acids in their DHR-2 (DOCK homology region 2) domains that are required for GEF activity, and bind directly to WAVE proteins [Wiskott-Aldrich syndrome protein (WASP) family Verprolin-homologous proteins] via their DHR-1 domains. The DOCK3 induces axonal outgrowth in CNS by stimulating membrane recruitment of the WAVE complex and activating the small G protein Rac1. This gene is associated with an attention deficit hyperactivity disorder-like phenotype by a complex chromosomal rearrangement. [provided by RefSeq, Aug 2010]
PHENOTYPE: Mice homozygous for a null allele exhibit abnormal behaviors and muscular weakness associated with axonal dystrophy. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dock3||
(F):5'- CTTGTCTCACAAGACGTAGGATGCC -3'
(R):5'- ATCTCCTTCAAGTCCCACAATGCTG -3'
(F):5'- CTGTACTGAGAAACTTGCTAGGC -3'
(R):5'- GCTGCTATCTTCTATCAGTAGCTAGG -3'