Incidental Mutation 'R1141:Zfp174'
ID102127
Institutional Source Beutler Lab
Gene Symbol Zfp174
Ensembl Gene ENSMUSG00000054939
Gene Namezinc finger protein 174
SynonymsLOC385674
MMRRC Submission 039214-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.232) question?
Stock #R1141 (G1)
Quality Score225
Status Not validated
Chromosome16
Chromosomal Location3847268-3858880 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 3849457 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Asparagine at position 170 (I170N)
Ref Sequence ENSEMBL: ENSMUSP00000045805 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000041778] [ENSMUST00000214238] [ENSMUST00000214590]
Predicted Effect probably benign
Transcript: ENSMUST00000041778
AA Change: I170N

PolyPhen 2 Score 0.073 (Sensitivity: 0.93; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000045805
Gene: ENSMUSG00000054939
AA Change: I170N

DomainStartEndE-ValueType
SCAN 42 154 6.21e-65 SMART
low complexity region 298 311 N/A INTRINSIC
ZnF_C2H2 326 348 5.99e-4 SMART
ZnF_C2H2 354 376 1.36e-2 SMART
ZnF_C2H2 382 404 1.43e-1 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000214238
Predicted Effect probably benign
Transcript: ENSMUST00000214590
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.0%
  • 10x: 94.1%
  • 20x: 84.2%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein with three Cys2-His2-type zinc fingers in the carboxy-terminus, a putative nuclear localization signal, and an amino-terminus SCAN box which forms homodimers. This protein is believed to function as a transcriptional repressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Dec 2016]
Allele List at MGI
Other mutations in this stock
Total: 26 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aak1 G A 6: 86,965,476 probably null Het
Asap2 A G 12: 21,185,110 N71S probably damaging Het
Brinp1 T C 4: 68,792,978 H331R probably benign Het
Brinp2 C A 1: 158,247,270 C427F probably damaging Het
Ccr6 A G 17: 8,256,002 Y13C probably damaging Het
Celf4 T C 18: 25,504,904 D187G probably damaging Het
Clcn6 T G 4: 148,013,899 T556P probably damaging Het
Dst A T 1: 34,188,696 Q1968L possibly damaging Het
Elavl4 G A 4: 110,251,368 Q53* probably null Het
Fbxw15 G A 9: 109,558,246 S227F probably damaging Het
Gli2 T C 1: 118,837,937 H828R possibly damaging Het
Gm12166 G A 11: 46,051,954 A114V possibly damaging Het
Gpr108 A G 17: 57,237,219 V397A probably damaging Het
Lrba T C 3: 86,619,558 I2241T probably damaging Het
Map3k21 G A 8: 125,941,732 V686M probably benign Het
Nfatc4 A T 14: 55,832,631 E752V probably damaging Het
R3hdm1 G A 1: 128,231,405 R939H probably benign Het
Rnf213 A G 11: 119,435,983 T1599A probably benign Het
Slc3a1 T C 17: 85,028,649 F73S possibly damaging Het
Sppl3 TGG TG 5: 115,088,293 probably null Het
Stx1b T C 7: 127,810,926 probably null Het
Tnc C T 4: 64,013,994 V728M probably damaging Het
Trim24 C T 6: 37,915,293 H254Y probably damaging Het
Ttbk2 A G 2: 120,806,851 L42P probably damaging Het
Vmn2r53 G A 7: 12,600,746 T329I possibly damaging Het
Zfp455 T A 13: 67,198,591 L22Q probably damaging Het
Other mutations in Zfp174
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01603:Zfp174 APN 16 3854289 missense probably benign
R0558:Zfp174 UTSW 16 3848254 missense possibly damaging 0.56
R1378:Zfp174 UTSW 16 3849489 missense probably benign 0.15
R1846:Zfp174 UTSW 16 3854735 missense probably benign 0.14
R2089:Zfp174 UTSW 16 3854642 missense possibly damaging 0.93
R2091:Zfp174 UTSW 16 3854642 missense possibly damaging 0.93
R2091:Zfp174 UTSW 16 3854642 missense possibly damaging 0.93
R5784:Zfp174 UTSW 16 3854574 missense probably benign 0.01
R6621:Zfp174 UTSW 16 3847955 missense probably damaging 1.00
R6710:Zfp174 UTSW 16 3848057 missense probably damaging 1.00
R6976:Zfp174 UTSW 16 3847940 missense possibly damaging 0.92
R7104:Zfp174 UTSW 16 3854405 missense not run
Predicted Primers
Posted On2014-01-15