Mutagenetix > Incidental Mutation

Incidental Mutation 'R1188:Gpr20'

102387

Institutional Source

Beutler Lab

Gene Symbol

Gpr20

Ensembl Gene

ENSMUSG00000045281

Gene Name

G protein-coupled receptor 20

Synonyms

A430106B11Rik

MMRRC Submission

039260-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1188 (G1)

Quality Score

123

Status

Not validated

Chromosome

Chromosomal Location

73566453-73579354 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 73567617 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 257 (H257Q)

Ref Sequence

ENSEMBL: ENSMUSP00000063910 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064166]

AlphaFold

Q8BYC4

Predicted Effect

probably damaging
Transcript: ENSMUST00000064166
AA Change: H257Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000063910
Gene: ENSMUSG00000045281
AA Change: H257Q

Domain	Start	End	E-Value	Type
Pfam:7tm_1	68	296	7.7e-32	PFAM

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 95.0%
20x: 88.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130230L23Rik	T	C	5: 66,147,741 (GRCm39)	Y14C	unknown	Het
Afap1l2	T	C	19: 56,913,501 (GRCm39)	K312E	probably damaging	Het
Amigo2	T	A	15: 97,143,594 (GRCm39)	E276V	probably benign	Het
Atf2	A	G	2: 73,675,881 (GRCm39)	F114L	probably damaging	Het
Avpr1a	G	T	10: 122,284,824 (GRCm39)	G39C	possibly damaging	Het
Ccpg1	A	G	9: 72,919,788 (GRCm39)	R468G	possibly damaging	Het
Celf6	G	T	9: 59,497,961 (GRCm39)	R130L	probably benign	Het
Dsp	T	C	13: 38,378,939 (GRCm39)	S1296P	probably damaging	Het
Fnip2	C	T	3: 79,369,469 (GRCm39)	R1072H	probably damaging	Het
Fsip2	G	T	2: 82,805,361 (GRCm39)	C560F	possibly damaging	Het
Get3	A	G	8: 85,746,422 (GRCm39)	I142T	probably damaging	Het
Gys2	T	A	6: 142,400,909 (GRCm39)	H297L	probably damaging	Het
Habp2	G	A	19: 56,300,154 (GRCm39)	S201N	probably benign	Het
Hars2	T	C	18: 36,921,022 (GRCm39)	I198T	probably damaging	Het
Jag2	G	A	12: 112,883,741 (GRCm39)	Q247*	probably null	Het
Jam2	A	G	16: 84,603,755 (GRCm39)	T81A	probably damaging	Het
Mrps26	G	A	2: 130,406,301 (GRCm39)	E145K	probably damaging	Het
Nup210l	T	C	3: 90,105,486 (GRCm39)	F1545L	probably benign	Het
Or10d4c	A	G	9: 39,558,772 (GRCm39)	Y250C	probably damaging	Het
Pikfyve	T	A	1: 65,286,118 (GRCm39)	V1074D	possibly damaging	Het
Prkag1	T	A	15: 98,712,479 (GRCm39)	I118F	probably damaging	Het
R3hdm2	G	A	10: 127,288,624 (GRCm39)	V91I	probably benign	Het
Rnf168	T	C	16: 32,117,477 (GRCm39)	V346A	probably benign	Het
Slc17a7	T	C	7: 44,819,311 (GRCm39)	V129A	possibly damaging	Het
Snai3	T	A	8: 123,181,701 (GRCm39)	Q252L	probably damaging	Het
Snx17	T	C	5: 31,353,166 (GRCm39)	V133A	probably benign	Het
Stt3a	A	G	9: 36,662,636 (GRCm39)	S59P	probably damaging	Het
Sun1	C	T	5: 139,224,611 (GRCm39)	R546C	probably damaging	Het
Thsd4	T	A	9: 60,301,689 (GRCm39)	Q202L	probably benign	Het
Tnrc6b	A	G	15: 80,763,430 (GRCm39)	T311A	probably benign	Het
Tshr	T	A	12: 91,468,942 (GRCm39)	D18E	probably benign	Het
Ttn	A	T	2: 76,619,773 (GRCm39)	L15965Q	probably damaging	Het
Wnk1	C	A	6: 119,925,670 (GRCm39)	E1265*	probably null	Het
Zbtb39	C	G	10: 127,578,175 (GRCm39)	Q250E	probably benign	Het

Other mutations in Gpr20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01759:Gpr20	APN	15	73,568,269 (GRCm39)	missense	probably damaging	1.00
IGL02312:Gpr20	APN	15	73,568,275 (GRCm39)	missense	probably benign	0.00
I1329:Gpr20	UTSW	15	73,567,612 (GRCm39)	missense	probably damaging	1.00
R1604:Gpr20	UTSW	15	73,567,853 (GRCm39)	missense	probably damaging	0.98
R1687:Gpr20	UTSW	15	73,567,751 (GRCm39)	missense	probably benign	0.21
R4398:Gpr20	UTSW	15	73,568,125 (GRCm39)	missense	probably benign
R4436:Gpr20	UTSW	15	73,567,649 (GRCm39)	missense	probably benign	0.06
R4505:Gpr20	UTSW	15	73,568,321 (GRCm39)	missense	probably benign	0.03
R4615:Gpr20	UTSW	15	73,567,585 (GRCm39)	missense	probably benign
R4616:Gpr20	UTSW	15	73,567,585 (GRCm39)	missense	probably benign
R4617:Gpr20	UTSW	15	73,567,585 (GRCm39)	missense	probably benign
R4618:Gpr20	UTSW	15	73,567,585 (GRCm39)	missense	probably benign
R6120:Gpr20	UTSW	15	73,567,853 (GRCm39)	missense	probably damaging	0.98
R7224:Gpr20	UTSW	15	73,567,981 (GRCm39)	missense	probably damaging	1.00
R8313:Gpr20	UTSW	15	73,568,161 (GRCm39)	missense	probably damaging	1.00
R9525:Gpr20	UTSW	15	73,567,681 (GRCm39)	missense	probably benign	0.31

Predicted Primers

PCR Primer
(F):5'- ATGCTGACCACATCCATACTGCTG -3'
(R):5'- GATCATGCTGCCGTGTCTTTGC -3'

Sequencing Primer
(F):5'- GAATTCTTCTCCACGCTGGTAG -3'
(R):5'- TTGCTCTGACCGTTCTGG -3'

Posted On

2014-01-15