Mutagenetix > Incidental Mutation

Incidental Mutation 'R0027:Rad9b'

15076

Institutional Source

Beutler Lab

Gene Symbol

Rad9b

Ensembl Gene

ENSMUSG00000038569

Gene Name

RAD9 checkpoint clamp component B

Synonyms

A630082N15Rik

MMRRC Submission

038322-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0027 (G1) of strain 730

Quality Score

Status

Validated

Chromosome

Chromosomal Location

122461286-122492296 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 122489786 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000121020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000049009] [ENSMUST00000111729] [ENSMUST00000117263] [ENSMUST00000117868] [ENSMUST00000118765] [ENSMUST00000118830] [ENSMUST00000144268] [ENSMUST00000155671] [ENSMUST00000145821] [ENSMUST00000154686]

AlphaFold

Q6WBX7

Predicted Effect

probably benign
Transcript: ENSMUST00000049009

SMART Domains

Protein: ENSMUSP00000036177
Gene: ENSMUSG00000038569

Domain	Start	End	E-Value	Type
Pfam:Rad9	14	271	1.8e-91	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111729

SMART Domains

Protein: ENSMUSP00000107358
Gene: ENSMUSG00000029462

Domain	Start	End	E-Value	Type
low complexity region	26	42	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000117263

SMART Domains

Protein: ENSMUSP00000113868
Gene: ENSMUSG00000038569

Domain	Start	End	E-Value	Type
Pfam:Rad9	14	271	1.1e-93	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117868

SMART Domains

Protein: ENSMUSP00000113345
Gene: ENSMUSG00000029462

Domain	Start	End	E-Value	Type
Pfam:Metallophos_2	1	143	1.1e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000118765

SMART Domains

Protein: ENSMUSP00000112579
Gene: ENSMUSG00000029462

Domain	Start	End	E-Value	Type
PDB:1W24\|A	1	65	7e-42	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000118830

SMART Domains

Protein: ENSMUSP00000113525
Gene: ENSMUSG00000029462

Domain	Start	End	E-Value	Type
Pfam:Metallophos_2	6	162	1.6e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132785

Predicted Effect

probably benign
Transcript: ENSMUST00000144268

SMART Domains

Protein: ENSMUSP00000117334
Gene: ENSMUSG00000038569

Domain	Start	End	E-Value	Type
Pfam:Rad9	1	64	6.7e-17	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200653

Predicted Effect

probably benign
Transcript: ENSMUST00000155671

SMART Domains

Protein: ENSMUSP00000121020
Gene: ENSMUSG00000029462

Domain	Start	End	E-Value	Type
Pfam:Metallophos_2	1	158	3.4e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000145821

SMART Domains

Protein: ENSMUSP00000123593
Gene: ENSMUSG00000029462

Domain	Start	End	E-Value	Type
Pfam:Metallophos_2	11	124	1e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000154686

Predicted Effect

probably benign
Transcript: ENSMUST00000149600

SMART Domains

Protein: ENSMUSP00000120843
Gene: ENSMUSG00000038569

Domain	Start	End	E-Value	Type
Pfam:Rad9	1	85	4.3e-25	PFAM

Coding Region Coverage

1x: 77.1%
3x: 64.8%
10x: 37.0%
20x: 19.3%

Validation Efficiency

92% (60/65)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality, decreased forebrain and somite cell proliferation, and increased cellular sensitivity to gamma irradiation and mitomycin C. Mice heterozygous for a knock-out allele exhibit reduced embryonic, fetal, and early postnatal survival. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 18 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts12	A	T	15: 11,285,959 (GRCm39)	I723F	probably damaging	Het
Anapc1	G	T	2: 128,483,431 (GRCm39)	D1221E	possibly damaging	Het
Arhgef28	T	A	13: 98,082,204 (GRCm39)	E1201V	possibly damaging	Het
Cdkl3	C	T	11: 51,923,176 (GRCm39)		probably benign	Het
Col1a2	G	A	6: 4,518,822 (GRCm39)		probably benign	Het
Fam131b	T	A	6: 42,295,182 (GRCm39)	M304L	probably benign	Het
Ints15	G	A	5: 143,293,817 (GRCm39)	T220I	probably damaging	Het
Kif11	C	T	19: 37,395,431 (GRCm39)		probably benign	Het
Lrpprc	G	A	17: 85,074,435 (GRCm39)	R491*	probably null	Het
Mbtd1	T	C	11: 93,815,375 (GRCm39)	V321A	possibly damaging	Het
Mon2	G	A	10: 122,871,953 (GRCm39)	S357L	possibly damaging	Het
Nopchap1	G	A	10: 83,200,393 (GRCm39)		probably benign	Het
Papola	A	C	12: 105,799,395 (GRCm39)	S675R	probably benign	Het
Pcdh9	T	A	14: 94,126,081 (GRCm39)	I30F	probably null	Het
Prl6a1	T	A	13: 27,502,011 (GRCm39)	L126Q	probably damaging	Het
Snrnp40	T	A	4: 130,262,066 (GRCm39)	H151Q	probably damaging	Het
Stard9	A	T	2: 120,533,982 (GRCm39)	Q3413L	probably benign	Het
Ubr4	C	A	4: 139,127,704 (GRCm39)	N567K	probably damaging	Het

Other mutations in Rad9b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00780:Rad9b	APN	5	122,482,310 (GRCm39)	missense	probably benign	0.01
IGL02375:Rad9b	APN	5	122,471,405 (GRCm39)	missense	possibly damaging	0.71
R0027:Rad9b	UTSW	5	122,489,786 (GRCm39)	unclassified	probably benign
R0103:Rad9b	UTSW	5	122,469,590 (GRCm39)	missense	probably damaging	0.99
R0103:Rad9b	UTSW	5	122,469,590 (GRCm39)	missense	probably damaging	0.99
R0975:Rad9b	UTSW	5	122,472,320 (GRCm39)	splice site	probably null
R2006:Rad9b	UTSW	5	122,477,842 (GRCm39)	missense	possibly damaging	0.74
R2265:Rad9b	UTSW	5	122,489,405 (GRCm39)	missense	probably damaging	0.98
R4818:Rad9b	UTSW	5	122,477,839 (GRCm39)	missense	probably damaging	1.00
R5392:Rad9b	UTSW	5	122,489,641 (GRCm39)	missense	probably damaging	1.00
R6041:Rad9b	UTSW	5	122,489,415 (GRCm39)	missense	probably damaging	0.99
R6109:Rad9b	UTSW	5	122,482,360 (GRCm39)	missense	probably damaging	1.00
R6133:Rad9b	UTSW	5	122,477,831 (GRCm39)	missense	possibly damaging	0.79
R6566:Rad9b	UTSW	5	122,490,630 (GRCm39)	missense	probably damaging	1.00
R6695:Rad9b	UTSW	5	122,489,754 (GRCm39)	missense	probably damaging	1.00
R7295:Rad9b	UTSW	5	122,472,341 (GRCm39)	missense	possibly damaging	0.95
R7299:Rad9b	UTSW	5	122,490,677 (GRCm39)	missense	possibly damaging	0.87
R7301:Rad9b	UTSW	5	122,490,677 (GRCm39)	missense	possibly damaging	0.87
R8495:Rad9b	UTSW	5	122,471,096 (GRCm39)	splice site	probably null
R8954:Rad9b	UTSW	5	122,482,293 (GRCm39)	missense	probably benign	0.44
Z1088:Rad9b	UTSW	5	122,471,435 (GRCm39)	missense	possibly damaging	0.90

Posted On

2012-12-12