Mutagenetix > Incidental Mutation

Incidental Mutation 'R1254:Rnf151'

151398

Institutional Source

Beutler Lab

Gene Symbol

Rnf151

Ensembl Gene

ENSMUSG00000008482

Gene Name

ring finger protein 151

Synonyms

1700010O16Rik

MMRRC Submission

039321-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R1254 (G1)

Quality Score

223

Status

Not validated

Chromosome

Chromosomal Location

24934813-24937031 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 24936526 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 20 (C20*)

Ref Sequence

ENSEMBL: ENSMUSP00000008626 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000008626] [ENSMUST00000045602] [ENSMUST00000054289] [ENSMUST00000152407] [ENSMUST00000135708] [ENSMUST00000170715] [ENSMUST00000146867]

AlphaFold

Q9CQ29

Predicted Effect

probably null
Transcript: ENSMUST00000008626
AA Change: C20*

SMART Domains

Protein: ENSMUSP00000008626
Gene: ENSMUSG00000008482
AA Change: C20*

Domain	Start	End	E-Value	Type
RING	20	57	1.76e-5	SMART
Pfam:zf-TRAF	102	158	7.5e-9	PFAM
low complexity region	176	193	N/A	INTRINSIC
low complexity region	203	216	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000045602

SMART Domains

Protein: ENSMUSP00000043543
Gene: ENSMUSG00000040048

Domain	Start	End	E-Value	Type
low complexity region	11	25	N/A	INTRINSIC
Pfam:NDUFB10	42	168	2.1e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000054289

SMART Domains

Protein: ENSMUSP00000092502
Gene: ENSMUSG00000044533

Domain	Start	End	E-Value	Type
low complexity region	6	53	N/A	INTRINSIC
Pfam:Ribosomal_S5	102	166	5.7e-34	PFAM
Pfam:Ribosomal_S5_C	185	256	2.4e-30	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000104521

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122809

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129451

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129580

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144285

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129984

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141324

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000158630

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161706

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132803

Predicted Effect

probably benign
Transcript: ENSMUST00000152407

SMART Domains

Protein: ENSMUSP00000114529
Gene: ENSMUSG00000044533

Domain	Start	End	E-Value	Type
low complexity region	6	53	N/A	INTRINSIC
Pfam:Ribosomal_S5	101	167	9.2e-32	PFAM
Pfam:Ribosomal_S5_C	184	257	1.2e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000135708

SMART Domains

Protein: ENSMUSP00000120091
Gene: ENSMUSG00000040048

Domain	Start	End	E-Value	Type
low complexity region	11	25	N/A	INTRINSIC
Pfam:NDUFB10	54	149	7.1e-40	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000170715

SMART Domains

Protein: ENSMUSP00000131474
Gene: ENSMUSG00000044533

Domain	Start	End	E-Value	Type
low complexity region	6	53	N/A	INTRINSIC
Pfam:Ribosomal_S5	101	167	1.1e-31	PFAM
Pfam:Ribosomal_S5_C	184	257	1.3e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000146867

SMART Domains

Protein: ENSMUSP00000120715
Gene: ENSMUSG00000044533

Domain	Start	End	E-Value	Type
low complexity region	6	53	N/A	INTRINSIC
Pfam:Ribosomal_S5	102	166	1.7e-35	PFAM
Pfam:Ribosomal_S5_C	185	256	8.3e-32	PFAM

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 96.2%
20x: 92.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 21 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb10	C	T	8: 124,688,791 (GRCm39)	G495D	probably damaging	Het
Ccdc39	T	C	3: 33,880,629 (GRCm39)	K446R	probably damaging	Het
Coro2b	A	G	9: 62,336,247 (GRCm39)	L280P	probably damaging	Het
D430041D05Rik	T	G	2: 104,031,648 (GRCm39)	K1649N	probably damaging	Het
Duox2	T	A	2: 122,113,959 (GRCm39)	H1191L	probably damaging	Het
Etl4	A	G	2: 20,812,734 (GRCm39)	T1430A	probably damaging	Het
Hivep3	A	G	4: 119,956,490 (GRCm39)	Y1602C	probably damaging	Het
Ints6	A	G	14: 62,953,823 (GRCm39)	S180P	probably benign	Het
Jarid2	T	A	13: 45,059,752 (GRCm39)	N661K	probably damaging	Het
Klhdc3	C	T	17: 46,988,993 (GRCm39)	V66I	probably benign	Het
Or5p56	T	A	7: 107,589,647 (GRCm39)	V25D	probably benign	Het
Pank1	T	C	19: 34,818,260 (GRCm39)	H93R	probably benign	Het
Pcdhb16	T	G	18: 37,612,348 (GRCm39)	V436G	possibly damaging	Het
Plekha6	G	A	1: 133,200,327 (GRCm39)	R302H	probably benign	Het
Ptk2	C	T	15: 73,101,819 (GRCm39)	R797Q	probably benign	Het
Rnase10	A	T	14: 51,247,083 (GRCm39)	M117L	probably damaging	Het
Robo4	T	C	9: 37,322,136 (GRCm39)		probably null	Het
Rps6ka5	G	T	12: 100,585,788 (GRCm39)	H168Q	probably damaging	Het
Sstr1	G	A	12: 58,260,108 (GRCm39)	V244M	possibly damaging	Het
Tmem147	A	T	7: 30,428,795 (GRCm39)	Y17*	probably null	Het
Vmn1r7	A	G	6: 57,001,772 (GRCm39)	C163R	probably damaging	Het

Other mutations in Rnf151

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0563:Rnf151	UTSW	17	24,936,430 (GRCm39)	unclassified	probably benign
R2921:Rnf151	UTSW	17	24,935,235 (GRCm39)	missense	possibly damaging	0.63
R5930:Rnf151	UTSW	17	24,937,004 (GRCm39)	splice site	probably null
R6515:Rnf151	UTSW	17	24,935,391 (GRCm39)	missense	probably benign	0.09
R8105:Rnf151	UTSW	17	24,935,400 (GRCm39)	missense	probably benign	0.43
X0025:Rnf151	UTSW	17	24,936,450 (GRCm39)	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- AACCCAGCTTTTGGTAGACTTTCCC -3'
(R):5'- GTACTCAATTCCAGGCAGATGCCC -3'

Sequencing Primer
(F):5'- GGTAGACTTTCCCATTCTTGGC -3'
(R):5'- GAAACATTCCAGCTTCTTCAGG -3'

Posted On

2014-01-29