Mutagenetix > Incidental Mutation

Incidental Mutation 'R1224:Map3k7cl'

152848

Institutional Source

Beutler Lab

Gene Symbol

Map3k7cl

Ensembl Gene

ENSMUSG00000025610

Gene Name

Map3k7 C-terminal like

Synonyms

ORF63

MMRRC Submission

039293-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R1224 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

87350218-87392224 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 87352891 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 21 (D21E)

Ref Sequence

ENSEMBL: ENSMUSP00000026700 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026700]

AlphaFold

P58500

Predicted Effect

probably benign
Transcript: ENSMUST00000026700
AA Change: D21E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000026700
Gene: ENSMUSG00000025610
AA Change: D21E

Domain	Start	End	E-Value	Type
low complexity region	37	48	N/A	INTRINSIC
coiled coil region	64	132	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	T	A	11: 109,931,408 (GRCm39)	E1248D	probably damaging	Het
Abcb10	C	T	8: 124,688,791 (GRCm39)	G495D	probably damaging	Het
Aldh16a1	T	C	7: 44,791,471 (GRCm39)		probably null	Het
Aldh9a1	T	A	1: 167,180,227 (GRCm39)	I107N	probably damaging	Het
Atp6ap1l	G	A	13: 91,034,675 (GRCm39)	Q236*	probably null	Het
Ccdc39	T	C	3: 33,880,629 (GRCm39)	K446R	probably damaging	Het
Cd46	A	T	1: 194,744,706 (GRCm39)	I344K	possibly damaging	Het
Ces3a	A	T	8: 105,778,141 (GRCm39)	D204V	probably damaging	Het
Clstn3	T	C	6: 124,434,878 (GRCm39)	S346G	probably benign	Het
Cplane1	T	A	15: 8,207,869 (GRCm39)	C207S	probably benign	Het
Dock1	A	G	7: 134,710,548 (GRCm39)	D1190G	possibly damaging	Het
Gimap8	G	A	6: 48,627,629 (GRCm39)	S201N	probably benign	Het
Gm10153	A	G	7: 141,744,072 (GRCm39)	S19P	unknown	Het
Igfn1	A	G	1: 135,897,494 (GRCm39)	V1024A	probably benign	Het
Kcng3	A	G	17: 83,938,824 (GRCm39)	L75P	probably damaging	Het
Krt31	C	T	11: 99,940,690 (GRCm39)		probably null	Het
Ly6a	A	G	15: 74,868,327 (GRCm39)	V54A	possibly damaging	Het
Or8g18	G	C	9: 39,149,547 (GRCm39)	P58A	probably benign	Het
Rapsn	T	C	2: 90,873,543 (GRCm39)	L230P	probably damaging	Het
Rhog	C	A	7: 101,888,959 (GRCm39)	V165F	possibly damaging	Het
Slc44a4	T	C	17: 35,140,844 (GRCm39)	V313A	probably benign	Het
Sox14	G	C	9: 99,757,168 (GRCm39)	H190Q	probably damaging	Het
Sval2	G	A	6: 41,841,188 (GRCm39)	D103N	probably benign	Het
Tm9sf3	A	T	19: 41,211,634 (GRCm39)	V403D	probably damaging	Het
Tmem269	T	C	4: 119,074,323 (GRCm39)	K18R	probably benign	Het
Unc80	T	C	1: 66,511,139 (GRCm39)	F49S	probably damaging	Het
Zdhhc7	T	A	8: 120,809,311 (GRCm39)	T299S	probably benign	Het
Zfp52	T	C	17: 21,775,324 (GRCm39)	V6A	possibly damaging	Het

Other mutations in Map3k7cl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02349:Map3k7cl	APN	16	87,352,901 (GRCm39)	splice site	probably benign
R0372:Map3k7cl	UTSW	16	87,378,100 (GRCm39)	missense	probably damaging	1.00
R5975:Map3k7cl	UTSW	16	87,367,209 (GRCm39)	missense	probably benign	0.05
R7868:Map3k7cl	UTSW	16	87,378,100 (GRCm39)	missense	probably damaging	1.00
R9136:Map3k7cl	UTSW	16	87,391,227 (GRCm39)	missense	probably damaging	1.00
R9306:Map3k7cl	UTSW	16	87,391,149 (GRCm39)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- CCGGCTATGTACTTACAATGTGGGG -3'
(R):5'- AGTGTGGGAACACCAGTGAGACTC -3'

Sequencing Primer
(F):5'- GGGAAAGGCAACTTACTAAATCTCC -3'
(R):5'- aaggaggaagaaaggaaggaag -3'

Posted On

2014-01-29