Mutagenetix > Incidental Mutation

Incidental Mutation 'R1368:Rasl10b'

155890

Institutional Source

Beutler Lab

Gene Symbol

Rasl10b

Ensembl Gene

ENSMUSG00000020684

Gene Name

RAS-like, family 10, member B

Synonyms

B230331P10Rik, VTS58635

MMRRC Submission

039433-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.140) question?

Stock #

R1368 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

83299963-83311865 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 83308665 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000135803 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021022] [ENSMUST00000021022] [ENSMUST00000052521] [ENSMUST00000108140] [ENSMUST00000108140] [ENSMUST00000147301] [ENSMUST00000164425] [ENSMUST00000164425] [ENSMUST00000175848] [ENSMUST00000175848]

AlphaFold

Q5SSG5

Predicted Effect

probably null
Transcript: ENSMUST00000021022

SMART Domains

Protein: ENSMUSP00000021022
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000021022

SMART Domains

Protein: ENSMUSP00000021022
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000052521

SMART Domains

Protein: ENSMUSP00000051907
Gene: ENSMUSG00000020686

Domain	Start	End	E-Value	Type
low complexity region	4	19	N/A	INTRINSIC
CH	34	154	2.15e-15	SMART
low complexity region	167	200	N/A	INTRINSIC
Pfam:GAS2	206	274	1.2e-35	PFAM
low complexity region	444	460	N/A	INTRINSIC
low complexity region	760	775	N/A	INTRINSIC
low complexity region	817	827	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000108140

SMART Domains

Protein: ENSMUSP00000103775
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000108140

SMART Domains

Protein: ENSMUSP00000103775
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000147301

SMART Domains

Protein: ENSMUSP00000119454
Gene: ENSMUSG00000020686

Domain	Start	End	E-Value	Type
low complexity region	4	19	N/A	INTRINSIC
CH	34	154	2.15e-15	SMART
low complexity region	167	185	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000164425

SMART Domains

Protein: ENSMUSP00000132696
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	178	6.6e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000164425

SMART Domains

Protein: ENSMUSP00000132696
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	178	6.6e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000175848

SMART Domains

Protein: ENSMUSP00000135803
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	120	2.3e-12	PFAM
Pfam:Miro	6	123	8.2e-13	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000175848

SMART Domains

Protein: ENSMUSP00000135803
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	120	2.3e-12	PFAM
Pfam:Miro	6	123	8.2e-13	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

98% (55/56)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele have smaller than normal large dense-core vesicles in atrial myocytes and exhibit impaired atrial natriuretic peptide secretion. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	G	11: 9,241,836 (GRCm39)	D1233G	probably benign	Het
Abcc8	G	A	7: 45,772,284 (GRCm39)	R832W	probably damaging	Het
Atp10b	A	G	11: 43,092,981 (GRCm39)	T439A	probably damaging	Het
Brd10	C	A	19: 29,693,796 (GRCm39)	S1966I	probably damaging	Het
C130073F10Rik	T	A	4: 101,747,953 (GRCm39)	N74Y	possibly damaging	Het
Cct8	G	A	16: 87,288,200 (GRCm39)	S124L	probably damaging	Het
Cdh9	T	A	15: 16,848,568 (GRCm39)		probably benign	Het
Cep290	C	T	10: 100,330,828 (GRCm39)		probably benign	Het
Chrng	T	A	1: 87,133,575 (GRCm39)	L10H	probably damaging	Het
Cnn3	T	C	3: 121,250,786 (GRCm39)	L189S	probably benign	Het
Cog4	A	G	8: 111,585,157 (GRCm39)		probably benign	Het
Cxcl12	A	G	6: 117,153,111 (GRCm39)		probably benign	Het
Eif2b2	G	A	12: 85,270,230 (GRCm39)	A257T	probably damaging	Het
Entrep2	A	T	7: 64,469,625 (GRCm39)	V41E	probably damaging	Het
Fanca	G	A	8: 124,031,020 (GRCm39)		probably benign	Het
Fcgbpl1	A	G	7: 27,858,903 (GRCm39)	Q2341R	possibly damaging	Het
Fktn	G	A	4: 53,734,880 (GRCm39)	G173R	probably damaging	Het
Gabbr2	T	C	4: 46,674,464 (GRCm39)	N841S	probably benign	Het
Gm5622	T	A	14: 51,899,647 (GRCm39)	V167E	possibly damaging	Het
Gnaq	T	C	19: 16,355,651 (GRCm39)	V289A	probably benign	Het
Gpatch2l	T	G	12: 86,307,439 (GRCm39)	D272E	possibly damaging	Het
Gzmg	G	A	14: 56,395,263 (GRCm39)	T74I	probably benign	Het
Ikzf2	G	A	1: 69,578,474 (GRCm39)	A271V	possibly damaging	Het
Lig4	T	C	8: 10,021,176 (GRCm39)	D868G	possibly damaging	Het
Mfsd6	T	C	1: 52,747,764 (GRCm39)	E367G	possibly damaging	Het
Mpz	T	C	1: 170,987,533 (GRCm39)	L223P	probably damaging	Het
Muc17	T	C	5: 137,175,674 (GRCm39)		probably benign	Het
Or2f1b	C	A	6: 42,739,613 (GRCm39)	T209K	possibly damaging	Het
Pdcd2	G	T	17: 15,746,846 (GRCm39)	N104K	probably damaging	Het
Pigg	G	T	5: 108,465,154 (GRCm39)	G129V	probably damaging	Het
Ppp3cc	T	C	14: 70,483,311 (GRCm39)	Y254C	probably damaging	Het
Prl3b1	G	A	13: 27,427,848 (GRCm39)	A53T	probably benign	Het
Psg23	A	G	7: 18,348,645 (GRCm39)	V54A	probably benign	Het
Psmd3	A	G	11: 98,573,746 (GRCm39)	D64G	probably damaging	Het
Psmg2	CTTCAGTT	CTTCAGTTCAGTT	18: 67,779,095 (GRCm39)		probably null	Het
Ptgdr	T	A	14: 45,090,799 (GRCm39)	I320F	probably damaging	Het
Rad50	T	A	11: 53,574,072 (GRCm39)	K722*	probably null	Het
Rgs9	G	A	11: 109,138,977 (GRCm39)	S255L	probably benign	Het
Ror1	C	T	4: 100,298,334 (GRCm39)	P569L	possibly damaging	Het
Rsad2	T	C	12: 26,497,147 (GRCm39)		probably null	Het
Scn8a	A	G	15: 100,933,422 (GRCm39)	D1501G	probably damaging	Het
Sema3c	A	G	5: 17,883,330 (GRCm39)	T313A	possibly damaging	Het
Serpinc1	T	A	1: 160,821,094 (GRCm39)	F59L	probably damaging	Het
Sike1	A	G	3: 102,903,500 (GRCm39)	D63G	possibly damaging	Het
Slc25a11	T	A	11: 70,536,352 (GRCm39)		probably null	Het
Slc32a1	A	G	2: 158,453,240 (GRCm39)	M27V	probably benign	Het
Smc5	A	G	19: 23,187,807 (GRCm39)	V1003A	probably damaging	Het
Tll2	G	A	19: 41,108,667 (GRCm39)	R328C	probably damaging	Het
Topaz1	A	G	9: 122,577,315 (GRCm39)	E75G	possibly damaging	Het
Tspan3	A	T	9: 56,054,783 (GRCm39)	V48E	probably benign	Het
Ugt1a6b	T	C	1: 88,035,358 (GRCm39)	I232T	probably benign	Het
Unc79	A	G	12: 103,122,772 (GRCm39)	K2290E	probably damaging	Het
Vmn1r19	T	C	6: 57,381,656 (GRCm39)	F70L	probably benign	Het

Other mutations in Rasl10b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02003:Rasl10b	APN	11	83,308,679 (GRCm39)	missense	probably damaging	1.00
R0378:Rasl10b	UTSW	11	83,309,519 (GRCm39)	missense	probably damaging	1.00
R0772:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0773:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0829:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0830:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0831:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1363:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1364:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1365:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1366:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1367:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1389:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R3771:Rasl10b	UTSW	11	83,309,349 (GRCm39)	missense	probably benign	0.04
R4925:Rasl10b	UTSW	11	83,303,505 (GRCm39)	missense	probably damaging	1.00
R5625:Rasl10b	UTSW	11	83,309,640 (GRCm39)	missense	probably damaging	1.00
R8830:Rasl10b	UTSW	11	83,303,502 (GRCm39)	missense	probably damaging	1.00
R9526:Rasl10b	UTSW	11	83,303,590 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTCACAAAATAGTGTGGTGGGC -3'
(R):5'- TGTACCCCTCTTTTGGAGACAGGC -3'

Sequencing Primer
(F):5'- CCAGGAAGTAACCAGCTTGTG -3'
(R):5'- TTTTGGAGACAGGCCAGCATC -3'

Posted On

2014-02-11