|Institutional Source||Beutler Lab|
|Gene Name||regulator of G-protein signaling 16|
|Is this an essential gene?||Probably non essential (E-score: 0.073)|
|Stock #||R1355 (G1)|
|Chromosomal Location||153740349-153745468 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 153743668 bp|
|Amino Acid Change||Lysine to Methionine at position 140 (K140M)|
|Ref Sequence||ENSEMBL: ENSMUSP00000027748 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000027748]|
|Predicted Effect||probably damaging
AA Change: K140M
PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)
AA Change: K140M
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.0292|
|Coding Region Coverage||
|Validation Efficiency||100% (57/57)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased fatty acid oxidation and circulating ketone levels when fed a high-fat diet. Mice homozygous for a different knock-out allele exhibit impaired Th1 and Th2 chemotaxis and increased susceptibility toparasitic infection. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rgs16||
(F):5'- GGAACTGTCCTAGACCCGAAAAGC -3'
(R):5'- AGGCTCAAGTGTGTGAAGGCTCAG -3'
(F):5'- cacaacctcctttaactccatttc -3'
(R):5'- CGATAAGCTGGTGACTTGAGGAA -3'