Mutagenetix > Incidental Mutation

Incidental Mutation 'R1353:Vmn2r77'

157096

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r77

Ensembl Gene

ENSMUSG00000090949

Gene Name

vomeronasal 2, receptor 77

Synonyms

EG546983

MMRRC Submission

039418-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R1353 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86444349-86461240 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 86451394 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Valine at position 427 (F427V)

Ref Sequence

ENSEMBL: ENSMUSP00000129540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164996]

AlphaFold

L7N2B7

Predicted Effect

probably benign
Transcript: ENSMUST00000164996
AA Change: F427V

PolyPhen 2 Score 0.290 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: F427V

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	467	1.4e-30	PFAM
Pfam:NCD3G	510	562	1e-20	PFAM
Pfam:7tm_3	594	830	2.6e-52	PFAM

Coding Region Coverage

1x: 98.8%
3x: 97.6%
10x: 94.1%
20x: 86.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Astn2	T	C	4: 66,184,572 (GRCm39)	Q175R	unknown	Het
Capn9	A	C	8: 125,332,305 (GRCm39)		probably null	Het
Car9	G	T	4: 43,512,439 (GRCm39)		probably null	Het
Chd7	A	G	4: 8,839,556 (GRCm39)	N1364S	probably damaging	Het
Cmya5	A	T	13: 93,178,033 (GRCm39)	V3607E	probably damaging	Het
Crb2	T	C	2: 37,677,293 (GRCm39)	C262R	probably damaging	Het
Cyp2t4	A	G	7: 26,856,055 (GRCm39)	N204S	probably benign	Het
Epha1	T	C	6: 42,338,771 (GRCm39)	T676A	probably damaging	Het
Fancm	G	A	12: 65,134,944 (GRCm39)	V246M	probably damaging	Het
Fcrl5	T	C	3: 87,355,669 (GRCm39)	S461P	probably damaging	Het
Gen1	A	T	12: 11,293,220 (GRCm39)	L394Q	probably benign	Het
Hsdl2	G	T	4: 59,596,971 (GRCm39)		probably null	Het
Klri2	C	T	6: 129,716,049 (GRCm39)	G97S	probably damaging	Het
Lypla2	T	C	4: 135,697,778 (GRCm39)	I55V	probably null	Het
Malrd1	A	G	2: 16,132,779 (GRCm39)	D1900G	unknown	Het
Map1b	A	G	13: 99,563,834 (GRCm39)	S2378P	unknown	Het
Marchf3	A	T	18: 56,909,177 (GRCm39)		probably null	Het
Myom2	G	T	8: 15,156,424 (GRCm39)	W757L	probably damaging	Het
Nat10	T	A	2: 103,584,418 (GRCm39)	M120L	possibly damaging	Het
Ncam2	T	G	16: 80,997,803 (GRCm39)	M1R	probably null	Het
Ncoa4	G	T	14: 31,892,815 (GRCm39)	G33*	probably null	Het
Or1j1	C	A	2: 36,702,926 (GRCm39)	M59I	possibly damaging	Het
Or1x2	G	A	11: 50,917,833 (GRCm39)	M1I	probably null	Het
Or2a20	T	A	6: 43,194,624 (GRCm39)	M259K	probably benign	Het
Or4p21	T	C	2: 88,276,895 (GRCm39)	H129R	probably benign	Het
Or6c38	C	T	10: 128,929,733 (GRCm39)	V37I	probably benign	Het
Or8b41	A	C	9: 38,055,024 (GRCm39)	I198L	probably benign	Het
Osbpl8	A	G	10: 111,112,340 (GRCm39)	N485S	probably damaging	Het
Pkdrej	A	T	15: 85,703,119 (GRCm39)	V939E	probably damaging	Het
Plaa	T	C	4: 94,459,926 (GRCm39)	E607G	possibly damaging	Het
Rtel1	T	A	2: 180,991,024 (GRCm39)	C285S	probably benign	Het
Rtl1	A	C	12: 109,558,633 (GRCm39)	C1069G	probably benign	Het
Rtn4	C	A	11: 29,657,595 (GRCm39)	A583E	probably damaging	Het
Runx1	T	A	16: 92,485,939 (GRCm39)	R32*	probably null	Het
Sdcbp	A	G	4: 6,381,057 (GRCm39)	I67M	probably damaging	Het
Slc22a12	G	C	19: 6,587,812 (GRCm39)	L259V	possibly damaging	Het
Sptlc2	A	G	12: 87,388,520 (GRCm39)	S321P	probably damaging	Het
Tmem30c	C	T	16: 57,098,028 (GRCm39)	G131D	probably damaging	Het
Tnfaip2	A	T	12: 111,411,403 (GRCm39)	Q9L	probably damaging	Het
Ttn	T	C	2: 76,576,910 (GRCm39)	Y24661C	probably damaging	Het
Zcchc4	A	G	5: 52,964,419 (GRCm39)	I292V	probably benign	Het
Zfp354b	A	T	11: 50,814,240 (GRCm39)	H228Q	probably damaging	Het
Zfp84	T	A	7: 29,475,600 (GRCm39)	N97K	probably benign	Het
Zfp976	T	C	7: 42,265,442 (GRCm39)	D49G	probably damaging	Het
Zgrf1	C	T	3: 127,405,452 (GRCm39)	T1550I	probably damaging	Het

Other mutations in Vmn2r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r77	APN	7	86,449,975 (GRCm39)	missense	probably benign	0.06
IGL01105:Vmn2r77	APN	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
IGL01367:Vmn2r77	APN	7	86,461,124 (GRCm39)	missense	probably damaging	0.98
IGL01634:Vmn2r77	APN	7	86,460,857 (GRCm39)	missense	probably benign
IGL01805:Vmn2r77	APN	7	86,460,395 (GRCm39)	missense	probably benign	0.18
IGL01868:Vmn2r77	APN	7	86,452,224 (GRCm39)	missense	probably benign	0.00
IGL01980:Vmn2r77	APN	7	86,450,678 (GRCm39)	missense	probably benign	0.14
IGL02055:Vmn2r77	APN	7	86,450,763 (GRCm39)	missense	probably benign	0.00
IGL02066:Vmn2r77	APN	7	86,452,836 (GRCm39)	nonsense	probably null
IGL02185:Vmn2r77	APN	7	86,444,360 (GRCm39)	missense	unknown
IGL02200:Vmn2r77	APN	7	86,451,187 (GRCm39)	missense	probably benign	0.04
IGL02336:Vmn2r77	APN	7	86,451,224 (GRCm39)	missense	probably damaging	0.99
IGL02445:Vmn2r77	APN	7	86,452,848 (GRCm39)	nonsense	probably null
IGL02557:Vmn2r77	APN	7	86,444,342 (GRCm39)	unclassified	probably benign
IGL02659:Vmn2r77	APN	7	86,449,979 (GRCm39)	missense	probably benign	0.32
IGL02978:Vmn2r77	APN	7	86,460,555 (GRCm39)	missense	probably benign
IGL03180:Vmn2r77	APN	7	86,450,843 (GRCm39)	missense	possibly damaging	0.85
IGL03255:Vmn2r77	APN	7	86,461,131 (GRCm39)	missense	probably benign	0.04
IGL03273:Vmn2r77	APN	7	86,460,494 (GRCm39)	missense	probably damaging	0.99
R0046:Vmn2r77	UTSW	7	86,451,146 (GRCm39)	missense	possibly damaging	0.73
R0047:Vmn2r77	UTSW	7	86,460,858 (GRCm39)	missense	probably benign	0.01
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0389:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R0635:Vmn2r77	UTSW	7	86,460,383 (GRCm39)	missense	probably benign
R0689:Vmn2r77	UTSW	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
R0827:Vmn2r77	UTSW	7	86,451,224 (GRCm39)	missense	probably damaging	1.00
R1167:Vmn2r77	UTSW	7	86,450,954 (GRCm39)	missense	probably benign	0.02
R1228:Vmn2r77	UTSW	7	86,450,242 (GRCm39)	critical splice donor site	probably null
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1613:Vmn2r77	UTSW	7	86,460,356 (GRCm39)	missense	probably damaging	1.00
R1654:Vmn2r77	UTSW	7	86,461,123 (GRCm39)	missense	probably damaging	1.00
R1742:Vmn2r77	UTSW	7	86,444,543 (GRCm39)	missense	probably benign	0.35
R1827:Vmn2r77	UTSW	7	86,450,821 (GRCm39)	missense	probably damaging	0.99
R1911:Vmn2r77	UTSW	7	86,461,001 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R2008:Vmn2r77	UTSW	7	86,450,921 (GRCm39)	missense	probably benign	0.31
R2093:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R2143:Vmn2r77	UTSW	7	86,461,152 (GRCm39)	missense	probably damaging	1.00
R2269:Vmn2r77	UTSW	7	86,460,897 (GRCm39)	missense	probably benign	0.03
R2972:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R2974:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R3037:Vmn2r77	UTSW	7	86,450,191 (GRCm39)	missense	probably benign
R3694:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3695:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3805:Vmn2r77	UTSW	7	86,444,368 (GRCm39)	nonsense	probably null
R3870:Vmn2r77	UTSW	7	86,461,050 (GRCm39)	missense	probably damaging	1.00
R4732:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R4733:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R5009:Vmn2r77	UTSW	7	86,451,015 (GRCm39)	missense	possibly damaging	0.82
R5201:Vmn2r77	UTSW	7	86,460,846 (GRCm39)	missense	probably damaging	0.98
R5218:Vmn2r77	UTSW	7	86,451,341 (GRCm39)	missense	probably damaging	0.98
R5469:Vmn2r77	UTSW	7	86,451,271 (GRCm39)	missense	probably benign	0.01
R5673:Vmn2r77	UTSW	7	86,461,214 (GRCm39)	missense	probably benign	0.05
R5771:Vmn2r77	UTSW	7	86,461,235 (GRCm39)	missense	probably benign	0.06
R5832:Vmn2r77	UTSW	7	86,460,670 (GRCm39)	nonsense	probably null
R5899:Vmn2r77	UTSW	7	86,460,924 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r77	UTSW	7	86,450,878 (GRCm39)	missense	probably benign	0.00
R6182:Vmn2r77	UTSW	7	86,460,957 (GRCm39)	missense	probably damaging	1.00
R6326:Vmn2r77	UTSW	7	86,451,031 (GRCm39)	missense	probably benign
R6419:Vmn2r77	UTSW	7	86,460,767 (GRCm39)	missense	probably damaging	0.99
R6549:Vmn2r77	UTSW	7	86,450,065 (GRCm39)	missense	probably benign	0.06
R6874:Vmn2r77	UTSW	7	86,451,286 (GRCm39)	missense	probably benign	0.00
R6972:Vmn2r77	UTSW	7	86,452,202 (GRCm39)	missense	probably damaging	1.00
R7056:Vmn2r77	UTSW	7	86,451,023 (GRCm39)	missense	probably benign	0.06
R7185:Vmn2r77	UTSW	7	86,451,035 (GRCm39)	missense	probably benign	0.00
R7261:Vmn2r77	UTSW	7	86,460,518 (GRCm39)	nonsense	probably null
R7298:Vmn2r77	UTSW	7	86,449,979 (GRCm39)	missense	probably benign	0.00
R7662:Vmn2r77	UTSW	7	86,460,492 (GRCm39)	nonsense	probably null
R8182:Vmn2r77	UTSW	7	86,460,801 (GRCm39)	missense	probably damaging	1.00
R8327:Vmn2r77	UTSW	7	86,450,680 (GRCm39)	missense	probably benign	0.08
R8387:Vmn2r77	UTSW	7	86,450,947 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r77	UTSW	7	86,452,855 (GRCm39)	missense	probably benign
R8898:Vmn2r77	UTSW	7	86,444,430 (GRCm39)	missense	probably damaging	1.00
R8973:Vmn2r77	UTSW	7	86,452,150 (GRCm39)	missense	possibly damaging	0.93
R9258:Vmn2r77	UTSW	7	86,452,302 (GRCm39)	missense	possibly damaging	0.88
R9338:Vmn2r77	UTSW	7	86,460,994 (GRCm39)	missense	probably damaging	1.00
R9358:Vmn2r77	UTSW	7	86,452,236 (GRCm39)	missense	probably benign	0.00
R9377:Vmn2r77	UTSW	7	86,444,442 (GRCm39)	missense	probably benign	0.05
R9404:Vmn2r77	UTSW	7	86,451,247 (GRCm39)	missense	probably benign
R9673:Vmn2r77	UTSW	7	86,450,171 (GRCm39)	missense	possibly damaging	0.75
R9679:Vmn2r77	UTSW	7	86,460,741 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- TGCCACCACCTGATTGTAAAACACTG -3'
(R):5'- GATTGTGAAATGCTGACTTGGATGCC -3'

Sequencing Primer
(F):5'- GTAAAACACTGAAGAATTGTCCAAC -3'
(R):5'- GTTGGCTGTAACATAAATAATGACAC -3'

Posted On

2014-02-11