Mutagenetix > Incidental Mutation

Incidental Mutation 'R1307:Zfp985'

157824

Institutional Source

Beutler Lab

Gene Symbol

Zfp985

Ensembl Gene

ENSMUSG00000065999

Gene Name

zinc finger protein 985

Synonyms

Gm13154

MMRRC Submission

039373-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.624) question?

Stock #

R1307 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

147637734-147669655 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 147667704 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 191 (L191F)

Ref Sequence

ENSEMBL: ENSMUSP00000080438 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081742] [ENSMUST00000139784] [ENSMUST00000143885]

AlphaFold

A2A7A5

Predicted Effect

probably benign
Transcript: ENSMUST00000081742
AA Change: L191F

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000080438
Gene: ENSMUSG00000065999
AA Change: L191F

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART
ZnF_C2H2	238	260	8.34e-3	SMART
ZnF_C2H2	266	288	1.47e-3	SMART
ZnF_C2H2	294	316	2.36e-2	SMART
ZnF_C2H2	322	344	8.34e-3	SMART
ZnF_C2H2	350	372	7.67e-2	SMART
ZnF_C2H2	378	400	8.6e-5	SMART
ZnF_C2H2	406	428	8.6e-5	SMART
ZnF_C2H2	434	456	7.9e-4	SMART
ZnF_C2H2	462	484	1.95e-3	SMART
ZnF_C2H2	490	512	2.09e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000139784

SMART Domains

Protein: ENSMUSP00000123296
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143885

SMART Domains

Protein: ENSMUSP00000121177
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.6%
20x: 91.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ascc1	A	T	10: 59,848,321 (GRCm39)	I83L	probably benign	Het
Bcl2a1b	T	C	9: 89,081,543 (GRCm39)	V44A	probably damaging	Het
Bdp1	T	C	13: 100,186,271 (GRCm39)	D1727G	possibly damaging	Het
Cabp1	A	G	5: 115,310,965 (GRCm39)	F294L	probably damaging	Het
Coq8b	T	A	7: 26,950,016 (GRCm39)	M365K	probably damaging	Het
Gk2	A	T	5: 97,603,268 (GRCm39)	D523E	probably benign	Het
Havcr1	C	T	11: 46,647,097 (GRCm39)	T177I	probably damaging	Het
Kdm4a	T	C	4: 118,032,839 (GRCm39)	T76A	probably benign	Het
Lama4	A	T	10: 38,946,028 (GRCm39)	I804F	probably benign	Het
Ntn4	C	T	10: 93,543,215 (GRCm39)	R314W	probably damaging	Het
Pcdh10	A	G	3: 45,336,314 (GRCm39)	N876S	probably benign	Het
Polr3f	T	C	2: 144,375,113 (GRCm39)	V95A	probably damaging	Het
Ptk2	A	G	15: 73,163,895 (GRCm39)	V389A	probably benign	Het
Rhof	T	C	5: 123,258,378 (GRCm39)	E151G	probably damaging	Het
Slc25a28	T	C	19: 43,655,470 (GRCm39)	N135S	probably benign	Het
Sphk1	G	A	11: 116,426,928 (GRCm39)	V295I	probably benign	Het
Sv2b	G	T	7: 74,856,182 (GRCm39)	T36K	probably damaging	Het
Sybu	T	C	15: 44,538,786 (GRCm39)	E291G	probably damaging	Het
Tnc	T	C	4: 63,927,096 (GRCm39)	E810G	probably damaging	Het
Uba52	T	C	8: 70,961,166 (GRCm39)	H68R	probably damaging	Het
Unc79	A	G	12: 103,036,335 (GRCm39)	N552S	probably damaging	Het
Ush2a	T	C	1: 188,090,164 (GRCm39)	C416R	probably damaging	Het
Ush2a	T	C	1: 188,184,037 (GRCm39)	V1447A	probably damaging	Het

Other mutations in Zfp985

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0098:Zfp985	UTSW	4	147,661,566 (GRCm39)	missense	probably damaging	0.97
R0324:Zfp985	UTSW	4	147,667,314 (GRCm39)	missense	probably benign	0.00
R1594:Zfp985	UTSW	4	147,667,537 (GRCm39)	missense	probably benign	0.05
R1657:Zfp985	UTSW	4	147,668,567 (GRCm39)	missense	probably benign	0.01
R1667:Zfp985	UTSW	4	147,668,407 (GRCm39)	missense	possibly damaging	0.84
R1761:Zfp985	UTSW	4	147,668,502 (GRCm39)	missense	probably benign	0.00
R1858:Zfp985	UTSW	4	147,667,315 (GRCm39)	missense	probably benign	0.29
R2509:Zfp985	UTSW	4	147,667,443 (GRCm39)	missense	possibly damaging	0.75
R2510:Zfp985	UTSW	4	147,667,443 (GRCm39)	missense	possibly damaging	0.75
R2847:Zfp985	UTSW	4	147,667,468 (GRCm39)	nonsense	probably null
R2848:Zfp985	UTSW	4	147,667,468 (GRCm39)	nonsense	probably null
R4245:Zfp985	UTSW	4	147,667,396 (GRCm39)	missense	probably damaging	0.96
R4260:Zfp985	UTSW	4	147,668,029 (GRCm39)	missense	probably damaging	1.00
R4434:Zfp985	UTSW	4	147,668,368 (GRCm39)	missense	probably benign	0.37
R4480:Zfp985	UTSW	4	147,668,536 (GRCm39)	missense	probably benign	0.07
R4512:Zfp985	UTSW	4	147,668,020 (GRCm39)	missense	probably damaging	1.00
R4514:Zfp985	UTSW	4	147,668,020 (GRCm39)	missense	probably damaging	1.00
R4528:Zfp985	UTSW	4	147,667,347 (GRCm39)	missense	possibly damaging	0.49
R4836:Zfp985	UTSW	4	147,668,612 (GRCm39)	missense	probably damaging	0.97
R4884:Zfp985	UTSW	4	147,667,801 (GRCm39)	missense	probably benign	0.04
R5054:Zfp985	UTSW	4	147,667,438 (GRCm39)	missense	probably damaging	0.98
R5106:Zfp985	UTSW	4	147,668,612 (GRCm39)	missense	probably damaging	0.97
R5205:Zfp985	UTSW	4	147,667,368 (GRCm39)	missense	probably damaging	1.00
R5266:Zfp985	UTSW	4	147,667,289 (GRCm39)	critical splice acceptor site	probably null
R5468:Zfp985	UTSW	4	147,667,702 (GRCm39)	missense	probably benign
R5533:Zfp985	UTSW	4	147,667,440 (GRCm39)	nonsense	probably null
R6282:Zfp985	UTSW	4	147,667,805 (GRCm39)	missense	probably benign	0.00
R6303:Zfp985	UTSW	4	147,668,232 (GRCm39)	missense	probably benign	0.01
R6609:Zfp985	UTSW	4	147,668,124 (GRCm39)	missense	probably damaging	1.00
R6609:Zfp985	UTSW	4	147,667,578 (GRCm39)	missense	probably benign
R6722:Zfp985	UTSW	4	147,667,528 (GRCm39)	missense	probably benign	0.26
R6858:Zfp985	UTSW	4	147,667,764 (GRCm39)	nonsense	probably null
R7064:Zfp985	UTSW	4	147,667,573 (GRCm39)	missense	probably benign	0.20
R7216:Zfp985	UTSW	4	147,667,913 (GRCm39)	missense	probably damaging	1.00
R7471:Zfp985	UTSW	4	147,667,388 (GRCm39)	missense	possibly damaging	0.75
R7583:Zfp985	UTSW	4	147,667,946 (GRCm39)	nonsense	probably null
R7685:Zfp985	UTSW	4	147,667,331 (GRCm39)	missense	probably benign	0.00
R8242:Zfp985	UTSW	4	147,668,639 (GRCm39)	missense	possibly damaging	0.52
R8504:Zfp985	UTSW	4	147,667,883 (GRCm39)	missense	possibly damaging	0.70
R8780:Zfp985	UTSW	4	147,668,412 (GRCm39)	missense	possibly damaging	0.79
R8785:Zfp985	UTSW	4	147,668,080 (GRCm39)	missense	probably damaging	1.00
R9485:Zfp985	UTSW	4	147,668,280 (GRCm39)	missense	probably damaging	1.00
R9513:Zfp985	UTSW	4	147,667,999 (GRCm39)	missense	probably damaging	1.00
R9631:Zfp985	UTSW	4	147,665,742 (GRCm39)	missense	probably damaging	1.00
R9722:Zfp985	UTSW	4	147,667,618 (GRCm39)	missense	possibly damaging	0.63
R9786:Zfp985	UTSW	4	147,668,047 (GRCm39)	missense	probably benign
X0050:Zfp985	UTSW	4	147,667,728 (GRCm39)	frame shift	probably null

Predicted Primers

Posted On

2014-02-18