Mutagenetix > Incidental Mutation

Incidental Mutation 'R1311:Olfml1'

157933

Institutional Source

Beutler Lab

Gene Symbol

Olfml1

Ensembl Gene

ENSMUSG00000051041

Gene Name

olfactomedin-like 1

Synonyms

mONT2, ONT2, MVAL564

MMRRC Submission

039377-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R1311 (G1)

Quality Score

172

Status

Validated

Chromosome

Chromosomal Location

107166653-107190301 bp(+) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to C at 107167103 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000114029 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000120990]

AlphaFold

Q8BSH2

Predicted Effect

probably null
Transcript: ENSMUST00000120990

SMART Domains

Protein: ENSMUSP00000114029
Gene: ENSMUSG00000051041

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	78	105	N/A	INTRINSIC
low complexity region	115	132	N/A	INTRINSIC
Pfam:OLF	145	233	5.5e-19	PFAM

Meta Mutation Damage Score

0.9492

Coding Region Coverage

1x: 98.8%
3x: 97.7%
10x: 94.1%
20x: 86.2%

Validation Efficiency

98% (42/43)

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acvr1c	T	C	2: 58,170,261 (GRCm39)	Q449R	probably benign	Het
Cap1	A	G	4: 122,759,007 (GRCm39)	Y195H	possibly damaging	Het
Casp8ap2	T	A	4: 32,648,111 (GRCm39)	N1939K	probably damaging	Het
Cd209c	T	A	8: 3,995,908 (GRCm39)	M1L	probably benign	Het
Ckb	TCCACCACCA	TCCACCA	12: 111,636,079 (GRCm39)		probably benign	Het
Col13a1	A	G	10: 61,699,789 (GRCm39)		probably benign	Het
Dennd4a	T	C	9: 64,817,286 (GRCm39)	V1640A	probably benign	Het
Eml6	T	C	11: 29,781,088 (GRCm39)		probably benign	Het
Fat3	G	A	9: 15,932,706 (GRCm39)	T1409I	probably damaging	Het
Gm4884	G	C	7: 40,692,539 (GRCm39)	E169D	possibly damaging	Het
Gm5709	T	C	3: 59,526,100 (GRCm39)		noncoding transcript	Het
Htr2b	C	A	1: 86,038,346 (GRCm39)	A87S	probably damaging	Het
Kansl2	G	T	15: 98,426,797 (GRCm39)	H275N	possibly damaging	Het
Megf6	G	A	4: 154,348,239 (GRCm39)		probably null	Het
Mtpn	A	G	6: 35,489,185 (GRCm39)	I113T	possibly damaging	Het
Myh6	G	T	14: 55,183,822 (GRCm39)	A1704E	probably damaging	Het
Notum	C	T	11: 120,546,575 (GRCm39)		probably benign	Het
Nxpe2	T	C	9: 48,237,914 (GRCm39)	T114A	probably damaging	Het
Or14c41	T	A	7: 86,235,161 (GRCm39)	V226D	probably damaging	Het
Ptpn5	A	T	7: 46,728,980 (GRCm39)		probably benign	Het
Rapgef2	A	G	3: 78,990,854 (GRCm39)	F985L	probably benign	Het
Slc7a7	A	T	14: 54,610,487 (GRCm39)	Y386*	probably null	Het
Snph	G	T	2: 151,439,122 (GRCm39)	P36Q	probably damaging	Het
St18	T	C	1: 6,915,868 (GRCm39)	C838R	probably damaging	Het
Sucla2	C	T	14: 73,798,074 (GRCm39)		probably benign	Het
Supt7l	T	C	5: 31,677,605 (GRCm39)	Y187C	probably damaging	Het
Sycp2l	A	T	13: 41,288,661 (GRCm39)	K241*	probably null	Het
Tenm2	G	T	11: 35,959,421 (GRCm39)		probably benign	Het
Tfap4	A	G	16: 4,377,290 (GRCm39)		probably null	Het
Tmem132e	T	C	11: 82,335,122 (GRCm39)	Y643H	probably damaging	Het
Tmem200c	A	T	17: 69,147,758 (GRCm39)	S114C	probably damaging	Het
Ush2a	T	C	1: 188,679,342 (GRCm39)	I4850T	possibly damaging	Het
Zmym6	G	T	4: 127,017,151 (GRCm39)	L977F	probably damaging	Het

Other mutations in Olfml1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Olfml1	APN	7	107,189,364 (GRCm39)	missense	possibly damaging	0.80
IGL02355:Olfml1	APN	7	107,167,010 (GRCm39)	missense	probably benign	0.00
IGL02362:Olfml1	APN	7	107,167,010 (GRCm39)	missense	probably benign	0.00
IGL03218:Olfml1	APN	7	107,170,476 (GRCm39)	missense	possibly damaging	0.87
IGL03291:Olfml1	APN	7	107,189,436 (GRCm39)	missense	probably damaging	1.00
R0041:Olfml1	UTSW	7	107,189,393 (GRCm39)	missense	possibly damaging	0.81
R0041:Olfml1	UTSW	7	107,189,393 (GRCm39)	missense	possibly damaging	0.81
R0081:Olfml1	UTSW	7	107,170,506 (GRCm39)	missense	probably benign	0.08
R0524:Olfml1	UTSW	7	107,189,384 (GRCm39)	missense	probably damaging	1.00
R1548:Olfml1	UTSW	7	107,189,582 (GRCm39)	missense	possibly damaging	0.88
R1564:Olfml1	UTSW	7	107,170,346 (GRCm39)	missense	possibly damaging	0.89
R4347:Olfml1	UTSW	7	107,167,040 (GRCm39)	missense	probably benign	0.00
R4997:Olfml1	UTSW	7	107,170,413 (GRCm39)	missense	probably damaging	1.00
R6788:Olfml1	UTSW	7	107,167,075 (GRCm39)	missense	probably damaging	1.00
R7282:Olfml1	UTSW	7	107,189,530 (GRCm39)	missense	possibly damaging	0.85
R7703:Olfml1	UTSW	7	107,170,392 (GRCm39)	missense	probably damaging	1.00
R7922:Olfml1	UTSW	7	107,170,356 (GRCm39)	missense	probably damaging	1.00
R8324:Olfml1	UTSW	7	107,189,570 (GRCm39)	missense	probably benign	0.04
R8441:Olfml1	UTSW	7	107,166,977 (GRCm39)	missense	probably benign	0.00
R9002:Olfml1	UTSW	7	107,189,423 (GRCm39)	missense	probably damaging	1.00
R9261:Olfml1	UTSW	7	107,167,007 (GRCm39)	missense	possibly damaging	0.88
R9797:Olfml1	UTSW	7	107,167,069 (GRCm39)	missense	probably benign	0.25

Predicted Primers

PCR Primer
(F):5'- TCTGGCTATGCAAAGCGTTGTTTTC -3'
(R):5'- CATCTGAGCTGCTAGGCTATGCAC -3'

Sequencing Primer
(F):5'- GTTTTCCAAGGAATTGCCACAG -3'
(R):5'- TGCTAGGCTATGCACATCTG -3'

Posted On

2014-02-18