Incidental Mutation 'R1297:Mettl25'
| ID |
158182 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mettl25
|
| Ensembl Gene |
ENSMUSG00000036009 |
| Gene Name |
methyltransferase like 25 |
| Synonyms |
BC067068 |
| MMRRC Submission |
039363-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.114)
|
| Stock # |
R1297 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
10 |
| Chromosomal Location |
105599050-105677241 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 105659126 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Serine to Glycine
at position 386
(S386G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000038665
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000046638]
[ENSMUST00000176040]
[ENSMUST00000176924]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000046638
AA Change: S386G
PolyPhen 2
Score 0.112 (Sensitivity: 0.93; Specificity: 0.86)
|
| SMART Domains |
Protein: ENSMUSP00000038665
Gene: ENSMUSG00000036009
AA Change: S386G
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Methyltransf_32
|
149 |
413 |
1.9e-30 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176040
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176924
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.0%
- 3x: 98.2%
- 10x: 95.8%
- 20x: 90.8%
|
| Validation Efficiency |
100% (37/37) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 31 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aebp1 |
A |
G |
11: 5,820,834 (GRCm39) |
N542D |
possibly damaging |
Het |
| Ap2b1 |
T |
A |
11: 83,223,935 (GRCm39) |
W217R |
probably damaging |
Het |
| Cep290 |
T |
A |
10: 100,374,962 (GRCm39) |
|
probably benign |
Het |
| Col27a1 |
G |
A |
4: 63,183,868 (GRCm39) |
|
probably benign |
Het |
| Cyp2d12 |
A |
T |
15: 82,441,887 (GRCm39) |
H109L |
probably benign |
Het |
| Dnah17 |
T |
C |
11: 118,012,192 (GRCm39) |
|
probably benign |
Het |
| Golga3 |
G |
A |
5: 110,352,709 (GRCm39) |
A867T |
probably benign |
Het |
| Gstt4 |
T |
A |
10: 75,653,133 (GRCm39) |
N143I |
possibly damaging |
Het |
| Hdac2 |
G |
A |
10: 36,862,370 (GRCm39) |
R78Q |
possibly damaging |
Het |
| Itsn2 |
T |
C |
12: 4,750,378 (GRCm39) |
I1241T |
probably damaging |
Het |
| Kalrn |
T |
C |
16: 33,836,868 (GRCm39) |
K2249R |
probably damaging |
Het |
| Klrg1 |
T |
A |
6: 122,250,538 (GRCm39) |
I138F |
probably benign |
Het |
| Mast1 |
A |
G |
8: 85,639,345 (GRCm39) |
V1328A |
probably benign |
Het |
| Nme2 |
A |
T |
11: 93,842,782 (GRCm39) |
N210K |
possibly damaging |
Het |
| Pgap1 |
T |
C |
1: 54,567,682 (GRCm39) |
S388G |
possibly damaging |
Het |
| Pgk2 |
C |
A |
17: 40,519,255 (GRCm39) |
V58L |
probably benign |
Het |
| Phf11 |
A |
T |
14: 59,495,996 (GRCm39) |
H39Q |
probably benign |
Het |
| Pou6f1 |
T |
A |
15: 100,476,186 (GRCm39) |
T292S |
probably damaging |
Het |
| Rbm5 |
G |
A |
9: 107,621,441 (GRCm39) |
R15C |
probably damaging |
Het |
| Rnf215 |
T |
C |
11: 4,089,806 (GRCm39) |
V273A |
possibly damaging |
Het |
| Rras |
A |
G |
7: 44,670,003 (GRCm39) |
D145G |
probably damaging |
Het |
| Safb2 |
T |
C |
17: 56,891,265 (GRCm39) |
|
probably benign |
Het |
| Setdb1 |
A |
T |
3: 95,257,187 (GRCm39) |
|
probably benign |
Het |
| Sp5 |
A |
G |
2: 70,306,873 (GRCm39) |
D186G |
probably benign |
Het |
| Thada |
A |
G |
17: 84,559,863 (GRCm39) |
|
probably benign |
Het |
| Tle1 |
A |
G |
4: 72,043,075 (GRCm39) |
V598A |
probably damaging |
Het |
| Tnrc6c |
A |
G |
11: 117,624,529 (GRCm39) |
N947S |
possibly damaging |
Het |
| Tnxb |
T |
C |
17: 34,929,140 (GRCm39) |
S2728P |
probably damaging |
Het |
| Vmn1r13 |
A |
G |
6: 57,187,392 (GRCm39) |
R184G |
probably damaging |
Het |
| Wdr24 |
C |
T |
17: 26,046,322 (GRCm39) |
T522I |
possibly damaging |
Het |
| Zfyve16 |
A |
G |
13: 92,658,840 (GRCm39) |
V357A |
probably benign |
Het |
|
| Other mutations in Mettl25 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00310:Mettl25
|
APN |
10 |
105,662,295 (GRCm39) |
missense |
probably benign |
|
|
IGL00698:Mettl25
|
APN |
10 |
105,629,201 (GRCm39) |
missense |
probably null |
0.31 |
|
IGL00766:Mettl25
|
APN |
10 |
105,615,443 (GRCm39) |
splice site |
probably benign |
|
|
IGL01360:Mettl25
|
APN |
10 |
105,659,058 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Mettl25
|
APN |
10 |
105,659,068 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02088:Mettl25
|
APN |
10 |
105,659,111 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02623:Mettl25
|
APN |
10 |
105,662,185 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL03245:Mettl25
|
APN |
10 |
105,662,358 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL03134:Mettl25
|
UTSW |
10 |
105,661,888 (GRCm39) |
nonsense |
probably null |
|
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1482:Mettl25
|
UTSW |
10 |
105,662,451 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1526:Mettl25
|
UTSW |
10 |
105,668,844 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R1542:Mettl25
|
UTSW |
10 |
105,661,981 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1589:Mettl25
|
UTSW |
10 |
105,615,493 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1901:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1902:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R2016:Mettl25
|
UTSW |
10 |
105,633,167 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2355:Mettl25
|
UTSW |
10 |
105,599,316 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2920:Mettl25
|
UTSW |
10 |
105,601,038 (GRCm39) |
splice site |
probably null |
|
|
R3149:Mettl25
|
UTSW |
10 |
105,662,214 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4547:Mettl25
|
UTSW |
10 |
105,661,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4646:Mettl25
|
UTSW |
10 |
105,662,416 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4652:Mettl25
|
UTSW |
10 |
105,615,509 (GRCm39) |
nonsense |
probably null |
|
|
R5020:Mettl25
|
UTSW |
10 |
105,662,068 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R5435:Mettl25
|
UTSW |
10 |
105,615,447 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6947:Mettl25
|
UTSW |
10 |
105,662,053 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7075:Mettl25
|
UTSW |
10 |
105,665,785 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7729:Mettl25
|
UTSW |
10 |
105,601,871 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8074:Mettl25
|
UTSW |
10 |
105,661,941 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8108:Mettl25
|
UTSW |
10 |
105,659,040 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8415:Mettl25
|
UTSW |
10 |
105,662,002 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8937:Mettl25
|
UTSW |
10 |
105,601,122 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8949:Mettl25
|
UTSW |
10 |
105,668,714 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9497:Mettl25
|
UTSW |
10 |
105,677,090 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9772:Mettl25
|
UTSW |
10 |
105,633,127 (GRCm39) |
missense |
probably benign |
|
|
RF009:Mettl25
|
UTSW |
10 |
105,669,100 (GRCm39) |
intron |
probably benign |
|
|
Z1176:Mettl25
|
UTSW |
10 |
105,661,959 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- aacaaaaCAAAGCAATCGTGTCACAGT -3'
(R):5'- TGAGCAGCCTAAAGAAGTTCAGCAAA -3'
Sequencing Primer
(F):5'- CGTGTCACAGTAATGATTACAAGAG -3'
(R):5'- CCATTTTGCCTAAAAGGAATTACTC -3'
|
| Posted On |
2014-02-18 |
Incidental Mutation