Mutagenetix > Incidental Mutation

Incidental Mutation 'R1435:Virma'

159430

Institutional Source

Beutler Lab

Gene Symbol

Virma

Ensembl Gene

ENSMUSG00000040720

Gene Name

vir like m6A methyltransferase associated

Synonyms

1110037F02Rik

MMRRC Submission

039490-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1435 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

11485958-11550684 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 11528621 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 1286 (A1286V)

Ref Sequence

ENSEMBL: ENSMUSP00000103943 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055372] [ENSMUST00000059914] [ENSMUST00000108307]

AlphaFold

A2AIV2

Predicted Effect

probably benign
Transcript: ENSMUST00000055372

SMART Domains

Protein: ENSMUSP00000063188
Gene: ENSMUSG00000040720

Domain	Start	End	E-Value	Type
low complexity region	139	153	N/A	INTRINSIC
low complexity region	172	198	N/A	INTRINSIC
low complexity region	236	267	N/A	INTRINSIC
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1008	1020	N/A	INTRINSIC
low complexity region	1112	1124	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000059914
AA Change: A1236V

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000058078
Gene: ENSMUSG00000040720
AA Change: A1236V

Domain	Start	End	E-Value	Type
low complexity region	139	153	N/A	INTRINSIC
low complexity region	172	198	N/A	INTRINSIC
low complexity region	236	267	N/A	INTRINSIC
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1008	1020	N/A	INTRINSIC
low complexity region	1112	1124	N/A	INTRINSIC
low complexity region	1224	1232	N/A	INTRINSIC
low complexity region	1443	1458	N/A	INTRINSIC
low complexity region	1460	1474	N/A	INTRINSIC
low complexity region	1618	1634	N/A	INTRINSIC
low complexity region	1684	1697	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
low complexity region	1796	1808	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000108307
AA Change: A1286V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000103943
Gene: ENSMUSG00000040720
AA Change: A1286V

Domain	Start	End	E-Value	Type
Pfam:VIR_N	5	266	2e-110	PFAM
low complexity region	276	297	N/A	INTRINSIC
low complexity region	615	625	N/A	INTRINSIC
low complexity region	1058	1070	N/A	INTRINSIC
low complexity region	1162	1174	N/A	INTRINSIC
low complexity region	1274	1282	N/A	INTRINSIC
low complexity region	1493	1508	N/A	INTRINSIC
low complexity region	1510	1524	N/A	INTRINSIC
low complexity region	1668	1684	N/A	INTRINSIC
low complexity region	1734	1747	N/A	INTRINSIC
low complexity region	1800	1807	N/A	INTRINSIC
low complexity region	1846	1858	N/A	INTRINSIC

Coding Region Coverage

1x: 98.7%
3x: 97.5%
10x: 93.0%
20x: 81.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acadvl	T	G	11: 69,905,642 (GRCm39)	T62P	probably benign	Het
Amz1	A	T	5: 140,733,921 (GRCm39)	N166Y	probably damaging	Het
Anxa6	T	C	11: 54,882,236 (GRCm39)	Q518R	probably benign	Het
Aox3	G	A	1: 58,202,605 (GRCm39)		probably null	Het
Arid1a	C	T	4: 133,408,009 (GRCm39)	R2166Q	unknown	Het
Asb4	T	G	6: 5,398,410 (GRCm39)	I125S	probably benign	Het
Aspscr1	T	C	11: 120,580,048 (GRCm39)	S196P	probably benign	Het
Atxn3	A	G	12: 101,908,460 (GRCm39)	F131L	probably benign	Het
B3gnt5	T	A	16: 19,587,924 (GRCm39)	Y48N	probably damaging	Het
Bglap3	A	G	3: 88,276,453 (GRCm39)	M35T	possibly damaging	Het
Cabp1	A	T	5: 115,311,267 (GRCm39)	D79E	probably damaging	Het
Cemip2	A	T	19: 21,822,070 (GRCm39)	Q1155L	probably benign	Het
Chd2	C	T	7: 73,102,884 (GRCm39)	R1367Q	probably damaging	Het
Clec5a	T	A	6: 40,561,358 (GRCm39)	Q29L	probably damaging	Het
Cmtr2	T	C	8: 110,947,711 (GRCm39)	L7P	probably benign	Het
Cnbd1	T	C	4: 18,907,026 (GRCm39)	I183V	probably benign	Het
Col7a1	G	A	9: 108,792,341 (GRCm39)	G1297D	unknown	Het
Csnk1g3	G	A	18: 54,039,746 (GRCm39)		probably null	Het
Cyp4a32	A	T	4: 115,463,863 (GRCm39)	N134I	probably damaging	Het
Cyp4f16	CTATG	CTATGTATG	17: 32,769,708 (GRCm39)		probably null	Het
Dst	G	A	1: 34,153,026 (GRCm39)	V56M	probably damaging	Het
Engase	A	T	11: 118,375,727 (GRCm39)	T32S	probably damaging	Het
Fam117b	T	C	1: 60,008,222 (GRCm39)	I352T	possibly damaging	Het
Golga4	C	A	9: 118,364,508 (GRCm39)	D290E	probably benign	Het
Gtf2a1l	C	A	17: 89,001,743 (GRCm39)	H153N	probably damaging	Het
Hivep1	C	A	13: 42,311,519 (GRCm39)	T1253N	probably damaging	Het
Hps1	T	C	19: 42,750,714 (GRCm39)	S398G	probably benign	Het
Il1r2	T	A	1: 40,144,459 (GRCm39)	F49I	probably damaging	Het
Iqsec1	A	G	6: 90,649,006 (GRCm39)	S808P	probably damaging	Het
Lrrk1	A	G	7: 65,922,776 (GRCm39)	L289P	probably damaging	Het
Magi2	T	A	5: 20,563,943 (GRCm39)	D358E	probably damaging	Het
Man2b2	A	G	5: 36,970,411 (GRCm39)	W832R	probably damaging	Het
Mfsd4a	G	T	1: 131,995,494 (GRCm39)	T46K	probably damaging	Het
N4bp3	G	A	11: 51,535,167 (GRCm39)	R341W	probably damaging	Het
Odad2	T	A	18: 7,222,646 (GRCm39)	H541L	probably benign	Het
Or51f2	T	C	7: 102,526,974 (GRCm39)	S216P	probably damaging	Het
Or8s5	A	T	15: 98,238,209 (GRCm39)	H220Q	possibly damaging	Het
Otof	A	G	5: 30,536,039 (GRCm39)	L1353S	probably benign	Het
Pcsk5	A	T	19: 17,541,246 (GRCm39)	C844*	probably null	Het
Pdk2	T	C	11: 94,922,721 (GRCm39)	Y153C	probably damaging	Het
Pes1	T	C	11: 3,926,075 (GRCm39)	V292A	probably benign	Het
Pex14	T	C	4: 149,047,984 (GRCm39)	T198A	probably benign	Het
Phactr4	G	A	4: 132,104,559 (GRCm39)	T256I	probably benign	Het
Pnpla2	G	A	7: 141,037,324 (GRCm39)	R109H	probably benign	Het
Polh	G	A	17: 46,505,181 (GRCm39)	T145I	probably damaging	Het
Polr3d	A	T	14: 70,677,479 (GRCm39)	V299E	probably benign	Het
Polr3e	C	T	7: 120,540,011 (GRCm39)	T586M	probably benign	Het
Rbm20	T	A	19: 53,802,588 (GRCm39)	F365L	probably benign	Het
Resf1	A	G	6: 149,227,580 (GRCm39)	T209A	probably benign	Het
Rnf213	G	T	11: 119,326,831 (GRCm39)	C1606F	probably damaging	Het
Sipa1l2	A	G	8: 126,195,464 (GRCm39)	V758A	probably damaging	Het
Slc22a16	T	A	10: 40,463,603 (GRCm39)	M451K	probably damaging	Het
Slc28a1	T	C	7: 80,803,265 (GRCm39)	S359P	probably damaging	Het
Slc45a1	G	T	4: 150,728,505 (GRCm39)	F99L	probably damaging	Het
Sp3	A	T	2: 72,768,500 (GRCm39)	N754K	possibly damaging	Het
Taf4b	A	T	18: 14,940,466 (GRCm39)	Q315L	probably damaging	Het
Tcstv2a	T	A	13: 120,725,524 (GRCm39)	W63R	probably damaging	Het
Tmprss15	A	T	16: 78,818,342 (GRCm39)	N544K	probably benign	Het
Tnfsf13b	A	G	8: 10,085,358 (GRCm39)	I283V	probably benign	Het
Tnfsf14	T	A	17: 57,497,605 (GRCm39)	E209V	possibly damaging	Het
Uckl1	T	G	2: 181,214,926 (GRCm39)	S283R	probably benign	Het
Ush1g	T	C	11: 115,209,294 (GRCm39)	D300G	probably damaging	Het
Vmn2r114	ATTT	ATT	17: 23,509,906 (GRCm39)		probably null	Het
Vmn2r59	A	T	7: 41,695,629 (GRCm39)	M261K	possibly damaging	Het
Vwf	A	T	6: 125,619,212 (GRCm39)	K1297*	probably null	Het
Zdbf2	G	A	1: 63,342,199 (GRCm39)	E193K	possibly damaging	Het
Zfp759	T	G	13: 67,286,830 (GRCm39)	I127S	possibly damaging	Het

Other mutations in Virma

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Virma	APN	4	11,519,424 (GRCm39)	splice site	probably benign
IGL00477:Virma	APN	4	11,519,006 (GRCm39)	missense	probably damaging	0.99
IGL01293:Virma	APN	4	11,521,114 (GRCm39)	missense	probably damaging	1.00
IGL01410:Virma	APN	4	11,518,929 (GRCm39)	nonsense	probably null
IGL01531:Virma	APN	4	11,528,753 (GRCm39)	missense	probably damaging	1.00
IGL01672:Virma	APN	4	11,527,792 (GRCm39)	missense	probably damaging	1.00
IGL01724:Virma	APN	4	11,528,672 (GRCm39)	missense	probably damaging	1.00
IGL01747:Virma	APN	4	11,526,877 (GRCm39)	missense	probably damaging	1.00
IGL01776:Virma	APN	4	11,527,792 (GRCm39)	missense	probably damaging	1.00
IGL02064:Virma	APN	4	11,513,163 (GRCm39)	missense	possibly damaging	0.87
IGL02243:Virma	APN	4	11,546,031 (GRCm39)	missense	probably damaging	1.00
IGL02244:Virma	APN	4	11,546,031 (GRCm39)	missense	probably damaging	1.00
IGL02445:Virma	APN	4	11,527,029 (GRCm39)	missense	probably damaging	0.97
IGL02546:Virma	APN	4	11,494,804 (GRCm39)	missense	probably damaging	0.99
IGL02807:Virma	APN	4	11,507,079 (GRCm39)	splice site	probably benign
IGL02967:Virma	APN	4	11,514,096 (GRCm39)	missense	probably benign	0.01
IGL03211:Virma	APN	4	11,548,770 (GRCm39)	nonsense	probably null
IGL03242:Virma	APN	4	11,527,669 (GRCm39)	missense	possibly damaging	0.70
IGL03256:Virma	APN	4	11,542,207 (GRCm39)	splice site	probably benign
IGL03327:Virma	APN	4	11,518,984 (GRCm39)	missense	probably benign	0.00
IGL03346:Virma	APN	4	11,518,984 (GRCm39)	missense	probably benign	0.00
PIT4802001:Virma	UTSW	4	11,546,008 (GRCm39)	missense	probably damaging	0.99
R0142:Virma	UTSW	4	11,548,783 (GRCm39)	missense	probably benign	0.04
R0355:Virma	UTSW	4	11,528,626 (GRCm39)	nonsense	probably null
R0522:Virma	UTSW	4	11,519,416 (GRCm39)	critical splice donor site	probably null
R0600:Virma	UTSW	4	11,498,769 (GRCm39)	missense	probably damaging	0.99
R1489:Virma	UTSW	4	11,521,164 (GRCm39)	missense	probably damaging	1.00
R1568:Virma	UTSW	4	11,528,776 (GRCm39)	missense	probably damaging	0.99
R1616:Virma	UTSW	4	11,544,954 (GRCm39)	missense	probably damaging	1.00
R1655:Virma	UTSW	4	11,494,786 (GRCm39)	missense	probably damaging	1.00
R1695:Virma	UTSW	4	11,494,814 (GRCm39)	missense	probably damaging	0.98
R1835:Virma	UTSW	4	11,540,511 (GRCm39)	missense	probably benign	0.02
R1951:Virma	UTSW	4	11,513,907 (GRCm39)	missense	probably benign	0.00
R1991:Virma	UTSW	4	11,519,242 (GRCm39)	missense	probably benign	0.06
R2145:Virma	UTSW	4	11,548,726 (GRCm39)	splice site	probably benign
R2172:Virma	UTSW	4	11,527,843 (GRCm39)	missense	possibly damaging	0.82
R2217:Virma	UTSW	4	11,544,924 (GRCm39)	missense	probably damaging	1.00
R2218:Virma	UTSW	4	11,544,924 (GRCm39)	missense	probably damaging	1.00
R2248:Virma	UTSW	4	11,518,927 (GRCm39)	missense	probably damaging	1.00
R2342:Virma	UTSW	4	11,501,316 (GRCm39)	missense	probably damaging	1.00
R3424:Virma	UTSW	4	11,513,177 (GRCm39)	nonsense	probably null
R4397:Virma	UTSW	4	11,513,901 (GRCm39)	missense	possibly damaging	0.81
R4449:Virma	UTSW	4	11,498,828 (GRCm39)	critical splice donor site	probably null
R4660:Virma	UTSW	4	11,513,505 (GRCm39)	missense	probably damaging	1.00
R4698:Virma	UTSW	4	11,528,636 (GRCm39)	missense	probably damaging	0.99
R4878:Virma	UTSW	4	11,544,971 (GRCm39)	missense	probably damaging	1.00
R4937:Virma	UTSW	4	11,521,147 (GRCm39)	nonsense	probably null
R5031:Virma	UTSW	4	11,542,116 (GRCm39)	nonsense	probably null
R5040:Virma	UTSW	4	11,528,746 (GRCm39)	missense	probably benign	0.01
R5061:Virma	UTSW	4	11,494,840 (GRCm39)	missense	possibly damaging	0.95
R5091:Virma	UTSW	4	11,519,392 (GRCm39)	missense	probably benign	0.00
R5137:Virma	UTSW	4	11,546,297 (GRCm39)	missense	probably damaging	1.00
R5262:Virma	UTSW	4	11,539,926 (GRCm39)	missense	probably benign	0.01
R5297:Virma	UTSW	4	11,494,819 (GRCm39)	missense	probably damaging	1.00
R5730:Virma	UTSW	4	11,542,154 (GRCm39)	missense	probably benign	0.44
R5818:Virma	UTSW	4	11,513,319 (GRCm39)	missense	possibly damaging	0.92
R5835:Virma	UTSW	4	11,514,036 (GRCm39)	missense	probably damaging	1.00
R6125:Virma	UTSW	4	11,521,172 (GRCm39)	missense	probably damaging	0.98
R6197:Virma	UTSW	4	11,505,498 (GRCm39)	missense	probably damaging	0.96
R6222:Virma	UTSW	4	11,527,820 (GRCm39)	missense	probably damaging	1.00
R6793:Virma	UTSW	4	11,539,968 (GRCm39)	missense	probably damaging	1.00
R7028:Virma	UTSW	4	11,519,249 (GRCm39)	missense	possibly damaging	0.50
R7356:Virma	UTSW	4	11,513,595 (GRCm39)	missense	probably damaging	0.99
R7383:Virma	UTSW	4	11,514,026 (GRCm39)	missense	probably damaging	0.98
R7391:Virma	UTSW	4	11,508,099 (GRCm39)	missense	probably damaging	0.99
R7425:Virma	UTSW	4	11,546,211 (GRCm39)	missense	possibly damaging	0.95
R7556:Virma	UTSW	4	11,518,927 (GRCm39)	missense	probably damaging	1.00
R7715:Virma	UTSW	4	11,513,016 (GRCm39)	splice site	probably null
R7715:Virma	UTSW	4	11,549,682 (GRCm39)	missense	probably damaging	1.00
R7986:Virma	UTSW	4	11,540,023 (GRCm39)	missense	probably benign	0.01
R7990:Virma	UTSW	4	11,513,983 (GRCm39)	missense	probably benign	0.00
R8048:Virma	UTSW	4	11,539,918 (GRCm39)	nonsense	probably null
R8050:Virma	UTSW	4	11,528,643 (GRCm39)	missense	probably benign	0.22
R8165:Virma	UTSW	4	11,542,128 (GRCm39)	missense	probably benign	0.00
R8412:Virma	UTSW	4	11,521,261 (GRCm39)	critical splice donor site	probably null
R8544:Virma	UTSW	4	11,516,949 (GRCm39)	missense	probably benign
R8551:Virma	UTSW	4	11,513,397 (GRCm39)	missense	probably damaging	1.00
R8699:Virma	UTSW	4	11,528,678 (GRCm39)	missense	probably benign	0.04
R8739:Virma	UTSW	4	11,540,643 (GRCm39)	critical splice donor site	probably null
R8950:Virma	UTSW	4	11,519,047 (GRCm39)	nonsense	probably null
R9015:Virma	UTSW	4	11,540,494 (GRCm39)	missense	probably benign	0.27
R9038:Virma	UTSW	4	11,526,922 (GRCm39)	missense	possibly damaging	0.93
R9115:Virma	UTSW	4	11,498,744 (GRCm39)	missense	probably benign	0.15
R9294:Virma	UTSW	4	11,513,507 (GRCm39)	nonsense	probably null
R9404:Virma	UTSW	4	11,513,626 (GRCm39)	missense	probably benign	0.17
R9477:Virma	UTSW	4	11,528,753 (GRCm39)	missense	probably damaging	1.00
R9532:Virma	UTSW	4	11,507,078 (GRCm39)	critical splice donor site	probably null
R9649:Virma	UTSW	4	11,486,045 (GRCm39)	start codon destroyed	probably null	0.08
R9657:Virma	UTSW	4	11,544,898 (GRCm39)	missense	probably damaging	0.99
R9780:Virma	UTSW	4	11,513,442 (GRCm39)	missense	possibly damaging	0.75
R9800:Virma	UTSW	4	11,546,007 (GRCm39)	missense	probably damaging	0.99
X0020:Virma	UTSW	4	11,486,055 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CCAACTGCTCTTCTGATCATGAGAACTG -3'
(R):5'- AAATGGATGTGACATACTCCACGCACT -3'

Sequencing Primer
(F):5'- agtcaccttcctgcccc -3'
(R):5'- ATACTCCACGCACTGCTGG -3'

Posted On

2014-03-14