Mutagenetix > Incidental Mutation

Incidental Mutation 'R1400:Or7g29'

160332

Institutional Source

Beutler Lab

Gene Symbol

Or7g29

Ensembl Gene

ENSMUSG00000059821

Gene Name

olfactory receptor family 7 subfamily G member 29

Synonyms

Olfr847, GA_x6K02T2PVTD-13113073-13112135, MOR149-2

MMRRC Submission

039462-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.132) question?

Stock #

R1400 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

19286237-19287175 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19286358 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 273 (V273A)

Ref Sequence

ENSEMBL: ENSMUSP00000151112 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079620] [ENSMUST00000216839]

AlphaFold

Q8VFF4

Predicted Effect

probably damaging
Transcript: ENSMUST00000079620
AA Change: V273A

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000078568
Gene: ENSMUSG00000059821
AA Change: V273A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.9e-56	PFAM
Pfam:7tm_1	41	290	2.1e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212306
AA Change: V273A

Predicted Effect

probably damaging
Transcript: ENSMUST00000216839
AA Change: V273A

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.3%
20x: 89.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130008F23Rik	T	C	17: 41,191,195 (GRCm39)	E78G	probably damaging	Het
Acsf2	T	C	11: 94,461,142 (GRCm39)	I345V	probably benign	Het
Akap11	T	A	14: 78,751,402 (GRCm39)	K328N	probably damaging	Het
Aoc1	T	A	6: 48,883,217 (GRCm39)	Y364*	probably null	Het
Aoc1	A	T	6: 48,883,645 (GRCm39)	Q507L	probably benign	Het
Atp6v1c2	T	C	12: 17,339,131 (GRCm39)	T207A	probably benign	Het
Atr	C	A	9: 95,744,901 (GRCm39)	Q73K	probably benign	Het
Cage1	A	G	13: 38,216,400 (GRCm39)	S17P	possibly damaging	Het
Cfap44	A	T	16: 44,241,575 (GRCm39)	I649F	probably benign	Het
Cops4	A	G	5: 100,681,412 (GRCm39)	K200R	probably damaging	Het
Crygd	A	G	1: 65,102,367 (GRCm39)	S32P	probably damaging	Het
Cyp3a11	A	G	5: 145,799,299 (GRCm39)	I296T	probably damaging	Het
Fads3	A	G	19: 10,033,664 (GRCm39)		probably null	Het
Fbn2	T	C	18: 58,213,265 (GRCm39)	E974G	possibly damaging	Het
Gcgr	A	G	11: 120,425,812 (GRCm39)	H45R	probably benign	Het
Gcn1	T	C	5: 115,752,220 (GRCm39)	I2112T	probably damaging	Het
Gm43302	A	T	5: 105,422,622 (GRCm39)	I470N	probably damaging	Het
Hoxa13	C	G	6: 52,260,647 (GRCm38)		probably benign	Het
Hoxa13	G	C	6: 52,260,648 (GRCm38)		probably benign	Het
Hsh2d	G	A	8: 72,954,304 (GRCm39)	D229N	probably benign	Het
Krt13	C	A	11: 100,012,110 (GRCm39)	G71V	probably damaging	Het
Las1l	T	C	X: 94,990,506 (GRCm39)	T390A	possibly damaging	Het
Lifr	T	A	15: 7,220,346 (GRCm39)	V992E	probably benign	Het
Mbd5	T	C	2: 49,164,788 (GRCm39)		probably null	Het
Mzf1	C	A	7: 12,786,698 (GRCm39)	R124L	possibly damaging	Het
Ndst3	A	G	3: 123,350,477 (GRCm39)	F636S	probably damaging	Het
Necab1	T	C	4: 14,975,185 (GRCm39)	D232G	possibly damaging	Het
Nlrp4e	A	T	7: 23,021,085 (GRCm39)	E524V	possibly damaging	Het
Nxf3	T	A	X: 134,976,794 (GRCm39)	T349S	probably benign	Het
Ofcc1	C	T	13: 40,362,305 (GRCm39)	G206R	probably benign	Het
Or4c12	T	A	2: 89,773,886 (GRCm39)	H191L	possibly damaging	Het
Or4n5	A	T	14: 50,133,148 (GRCm39)	I37K	possibly damaging	Het
Or9g19	T	C	2: 85,600,477 (GRCm39)	C111R	possibly damaging	Het
Plscr1l1	G	T	9: 92,233,180 (GRCm39)	C25F	probably benign	Het
Ppm1e	T	A	11: 87,122,592 (GRCm39)	N455I	probably damaging	Het
Prkag2	G	A	5: 25,078,916 (GRCm39)	T158I	probably damaging	Het
Ptpn18	T	C	1: 34,502,587 (GRCm39)		probably null	Het
Rai14	T	G	15: 10,571,634 (GRCm39)	K936N	probably damaging	Het
Rasgrf2	A	G	13: 92,035,808 (GRCm39)	L1077P	probably damaging	Het
Rgn	C	A	X: 20,416,696 (GRCm39)	Q27K	probably benign	Het
Ryr2	C	T	13: 11,609,962 (GRCm39)	S723N	probably benign	Het
Scamp1	A	G	13: 94,361,455 (GRCm39)	F142L	possibly damaging	Het
Selenon	A	G	4: 134,278,829 (GRCm39)	V67A	probably benign	Het
Slc5a5	T	C	8: 71,342,079 (GRCm39)	I292V	possibly damaging	Het
Smarca2	C	A	19: 26,654,140 (GRCm39)	T775K	probably damaging	Het
Stab1	C	T	14: 30,861,787 (GRCm39)	V2437I	possibly damaging	Het
Tas2r143	C	T	6: 42,377,317 (GRCm39)	A49V	probably benign	Het
Tlr7	T	A	X: 166,090,845 (GRCm39)	N214Y	probably damaging	Het
Unc13a	C	A	8: 72,103,865 (GRCm39)	D856Y	probably damaging	Het
Upp2	T	C	2: 58,680,118 (GRCm39)	Y263H	probably damaging	Het
Vill	T	C	9: 118,892,415 (GRCm39)	S349P	probably benign	Het
Zfp644	T	C	5: 106,785,336 (GRCm39)		probably null	Het
Zfp664	T	C	5: 124,963,217 (GRCm39)	C204R	unknown	Het
Zfp729b	A	G	13: 67,740,913 (GRCm39)	Y451H	possibly damaging	Het

Other mutations in Or7g29

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01150:Or7g29	APN	9	19,286,535 (GRCm39)	missense	probably damaging	1.00
IGL01293:Or7g29	APN	9	19,286,632 (GRCm39)	missense	probably benign	0.23
IGL01879:Or7g29	APN	9	19,286,703 (GRCm39)	nonsense	probably null
IGL03298:Or7g29	APN	9	19,286,358 (GRCm39)	missense	probably damaging	1.00
R1350:Or7g29	UTSW	9	19,286,710 (GRCm39)	missense	possibly damaging	0.94
R2894:Or7g29	UTSW	9	19,286,588 (GRCm39)	nonsense	probably null
R4468:Or7g29	UTSW	9	19,286,944 (GRCm39)	missense	probably benign	0.00
R4694:Or7g29	UTSW	9	19,286,694 (GRCm39)	missense	probably damaging	1.00
R4791:Or7g29	UTSW	9	19,287,105 (GRCm39)	missense	probably benign	0.28
R4794:Or7g29	UTSW	9	19,286,841 (GRCm39)	missense	probably benign	0.00
R5517:Or7g29	UTSW	9	19,287,063 (GRCm39)	missense	probably damaging	1.00
R5599:Or7g29	UTSW	9	19,286,925 (GRCm39)	missense	possibly damaging	0.77
R5777:Or7g29	UTSW	9	19,287,014 (GRCm39)	missense	probably benign	0.29
R6505:Or7g29	UTSW	9	19,286,237 (GRCm39)	makesense	probably null
R6509:Or7g29	UTSW	9	19,286,439 (GRCm39)	missense	probably benign
R7246:Or7g29	UTSW	9	19,286,761 (GRCm39)	nonsense	probably null
R7659:Or7g29	UTSW	9	19,286,854 (GRCm39)	missense	probably benign	0.03
R7789:Or7g29	UTSW	9	19,286,361 (GRCm39)	missense	probably benign	0.33
R7886:Or7g29	UTSW	9	19,287,202 (GRCm39)	splice site	probably null
R8948:Or7g29	UTSW	9	19,286,262 (GRCm39)	missense	probably benign	0.03
R9326:Or7g29	UTSW	9	19,286,346 (GRCm39)	missense	probably damaging	1.00
R9612:Or7g29	UTSW	9	19,286,677 (GRCm39)	missense	possibly damaging	0.69
R9784:Or7g29	UTSW	9	19,287,116 (GRCm39)	missense	probably damaging	0.99
R9798:Or7g29	UTSW	9	19,286,577 (GRCm39)	missense	probably benign	0.30
Z1088:Or7g29	UTSW	9	19,286,980 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGAGAACCTGACTTTGAACCAACACAT -3'
(R):5'- GCTTGCCTGTTCTGATACTCTCATCAAT -3'

Sequencing Primer
(F):5'- GGAAAACTCAAACATGCTTACTCATC -3'
(R):5'- GAATTCCAATCTCTGGAATCATTTTC -3'

Posted On

2014-03-14