Mutagenetix > Incidental Mutation

Incidental Mutation 'R1438:Vmn2r81'

160784

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r81

Ensembl Gene

ENSMUSG00000055515

Gene Name

vomeronasal 2, receptor 81

Synonyms

pheromone recepter, V2rf2, EC1-VR2

MMRRC Submission

039493-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R1438 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

79083611-79130369 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 79129691 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 861 (T861A)

Ref Sequence

ENSEMBL: ENSMUSP00000020547 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020547]

AlphaFold

Q80Z09

Predicted Effect

probably benign
Transcript: ENSMUST00000020547
AA Change: T861A

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000020547
Gene: ENSMUSG00000055515
AA Change: T861A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	7.7e-37	PFAM
Pfam:NCD3G	517	570	8.9e-21	PFAM
Pfam:7tm_3	603	838	6.1e-50	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 94.7%
20x: 87.3%

Validation Efficiency

99% (80/81)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit vomeronasal sensory neuron axons that spread out diffusely over the posterior accessory olfactory bulb and extend abnormally into the anterior olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actl6b	A	T	5: 137,552,871 (GRCm39)	I67F	probably damaging	Het
Adam24	A	T	8: 41,134,431 (GRCm39)	N633I	probably benign	Het
Adgrg6	T	C	10: 14,344,585 (GRCm39)	S123G	possibly damaging	Het
Afdn	T	A	17: 14,075,652 (GRCm39)	F940L	probably damaging	Het
Ahrr	A	T	13: 74,372,987 (GRCm39)	Y26*	probably null	Het
Akap1	C	A	11: 88,735,577 (GRCm39)	G362*	probably null	Het
Aox3	A	T	1: 58,192,337 (GRCm39)	T536S	probably benign	Het
Boc	A	T	16: 44,309,109 (GRCm39)		probably null	Het
Cchcr1	T	C	17: 35,841,457 (GRCm39)		probably null	Het
Cct2	A	T	10: 116,890,897 (GRCm39)		probably benign	Het
Cd84	A	T	1: 171,679,685 (GRCm39)	Y121F	probably damaging	Het
Cecr2	T	A	6: 120,738,433 (GRCm39)	C275*	probably null	Het
Chchd3	C	A	6: 32,985,503 (GRCm39)		probably benign	Het
Ckmt2	A	T	13: 92,007,971 (GRCm39)		probably benign	Het
Col5a3	T	C	9: 20,691,253 (GRCm39)	K1131E	probably damaging	Het
Dek	A	T	13: 47,241,647 (GRCm39)	S306T	probably benign	Het
Dhx32	T	C	7: 133,339,069 (GRCm39)	E322G	possibly damaging	Het
Dlg5	T	A	14: 24,204,673 (GRCm39)	D941V	possibly damaging	Het
Dnah10	A	G	5: 124,876,009 (GRCm39)	N2559S	probably benign	Het
Dnajc3	A	G	14: 119,205,518 (GRCm39)	T171A	probably benign	Het
Eftud2	G	T	11: 102,750,868 (GRCm39)	F308L	probably damaging	Het
Elp6	T	C	9: 110,143,123 (GRCm39)	F95S	probably damaging	Het
Emsy	C	T	7: 98,270,613 (GRCm39)	V450I	possibly damaging	Het
Exoc3	A	T	13: 74,338,298 (GRCm39)	M362K	probably damaging	Het
Fat2	A	T	11: 55,178,637 (GRCm39)	D1474E	probably damaging	Het
Fcgbp	T	A	7: 27,803,158 (GRCm39)	C1587*	probably null	Het
Fosl2	T	A	5: 32,304,329 (GRCm39)	L88Q	probably damaging	Het
Fsd2	T	C	7: 81,198,621 (GRCm39)	D381G	probably benign	Het
Golim4	A	C	3: 75,863,440 (GRCm39)	S56A	probably damaging	Het
Gpr39	T	C	1: 125,800,093 (GRCm39)		probably benign	Het
Gucy1b2	T	C	14: 62,651,770 (GRCm39)	I409V	probably damaging	Het
Hivep1	A	G	13: 42,311,596 (GRCm39)	T1279A	probably benign	Het
Ifit1bl2	T	A	19: 34,596,569 (GRCm39)	Q349L	possibly damaging	Het
Kcnc1	A	T	7: 46,077,691 (GRCm39)	I498F	possibly damaging	Het
Kctd21	T	A	7: 96,996,704 (GRCm39)	I59N	probably damaging	Het
Lama5	T	A	2: 179,824,593 (GRCm39)	T2577S	probably benign	Het
Mief2	G	T	11: 60,621,140 (GRCm39)	R9M	possibly damaging	Het
Mmp3	T	C	9: 7,453,705 (GRCm39)	V442A	probably benign	Het
Nkapd1	C	A	9: 50,518,972 (GRCm39)	K213N	possibly damaging	Het
Nrxn3	C	T	12: 90,298,909 (GRCm39)	R477W	probably damaging	Het
Or12e7	A	G	2: 87,288,336 (GRCm39)	T276A	probably benign	Het
Or7a41	T	A	10: 78,871,122 (GRCm39)	V164E	possibly damaging	Het
Parp1	A	G	1: 180,418,807 (GRCm39)	T656A	probably benign	Het
Pcdhb19	A	G	18: 37,631,015 (GRCm39)	D270G	probably damaging	Het
Phlpp1	A	G	1: 106,101,142 (GRCm39)	D470G	possibly damaging	Het
Prdm1	T	C	10: 44,318,124 (GRCm39)	E248G	probably benign	Het
Prtg	C	T	9: 72,818,032 (GRCm39)		probably benign	Het
Ptpn1	T	C	2: 167,818,529 (GRCm39)	Y424H	probably damaging	Het
Ptprr	T	C	10: 116,092,109 (GRCm39)	V369A	probably damaging	Het
Rai1	T	G	11: 60,076,221 (GRCm39)	V95G	probably benign	Het
Rasal3	T	A	17: 32,612,509 (GRCm39)		probably null	Het
Rbm19	A	G	5: 120,260,961 (GRCm39)	E195G	probably benign	Het
Rhbdd3	T	A	11: 5,053,332 (GRCm39)	L44Q	probably damaging	Het
Ripply2	T	C	9: 86,901,713 (GRCm39)	W80R	probably damaging	Het
Rnf183	A	G	4: 62,346,760 (GRCm39)	C13R	probably damaging	Het
Rorb	A	G	19: 18,932,417 (GRCm39)	L367P	probably damaging	Het
Rpl7a-ps5	C	T	17: 58,146,135 (GRCm39)		probably benign	Het
Rreb1	A	G	13: 38,114,581 (GRCm39)	N647D	probably benign	Het
Rtn1	A	T	12: 72,351,187 (GRCm39)	S341T	probably damaging	Het
Ryr3	T	C	2: 112,588,046 (GRCm39)	S2632G	probably benign	Het
Scube1	A	G	15: 83,499,227 (GRCm39)	C633R	possibly damaging	Het
Sdk1	A	T	5: 142,024,078 (GRCm39)	I723F	probably damaging	Het
Sec23a	A	T	12: 59,048,796 (GRCm39)	C109S	probably damaging	Het
Septin11	T	C	5: 93,296,287 (GRCm39)	F60L	probably damaging	Het
Sgf29	G	A	7: 126,271,063 (GRCm39)		probably null	Het
Skint5	A	G	4: 113,413,308 (GRCm39)		probably benign	Het
Smo	G	A	6: 29,755,482 (GRCm39)	V385I	possibly damaging	Het
Tada2a	G	A	11: 84,000,837 (GRCm39)	T76I	probably damaging	Het
Tas2r118	T	A	6: 23,969,422 (GRCm39)	H213L	possibly damaging	Het
Thoc5	A	T	11: 4,861,427 (GRCm39)		probably benign	Het
Tmem33	T	A	5: 67,424,634 (GRCm39)		probably null	Het
Top1mt	A	G	15: 75,546,247 (GRCm39)	L78P	probably damaging	Het
Uvssa	T	A	5: 33,571,228 (GRCm39)		probably benign	Het
Vmn2r50	A	T	7: 9,784,062 (GRCm39)	C137*	probably null	Het
Wnt3	A	T	11: 103,699,077 (GRCm39)	N61I	probably damaging	Het
Zswim9	A	G	7: 13,011,144 (GRCm39)	I68T	possibly damaging	Het
Zzef1	T	C	11: 72,803,771 (GRCm39)	I2535T	probably damaging	Het

Other mutations in Vmn2r81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01111:Vmn2r81	APN	10	79,083,831 (GRCm39)	missense	probably benign	0.20
IGL02255:Vmn2r81	APN	10	79,083,806 (GRCm39)	nonsense	probably null
IGL02901:Vmn2r81	APN	10	79,106,564 (GRCm39)	missense	probably damaging	0.98
R0383:Vmn2r81	UTSW	10	79,129,281 (GRCm39)	missense	possibly damaging	0.95
R0555:Vmn2r81	UTSW	10	79,129,283 (GRCm39)	missense	probably damaging	1.00
R0715:Vmn2r81	UTSW	10	79,104,434 (GRCm39)	missense	probably damaging	1.00
R0967:Vmn2r81	UTSW	10	79,083,857 (GRCm39)	splice site	probably benign
R1468:Vmn2r81	UTSW	10	79,129,496 (GRCm39)	missense	probably damaging	1.00
R1468:Vmn2r81	UTSW	10	79,129,496 (GRCm39)	missense	probably damaging	1.00
R1589:Vmn2r81	UTSW	10	79,128,858 (GRCm39)	missense	probably damaging	0.99
R1671:Vmn2r81	UTSW	10	79,103,265 (GRCm39)	missense	probably benign	0.04
R1672:Vmn2r81	UTSW	10	79,104,112 (GRCm39)	missense	probably damaging	0.99
R1728:Vmn2r81	UTSW	10	79,106,489 (GRCm39)	missense	probably benign	0.08
R1784:Vmn2r81	UTSW	10	79,106,489 (GRCm39)	missense	probably benign	0.08
R1931:Vmn2r81	UTSW	10	79,129,328 (GRCm39)	missense	probably damaging	0.98
R1934:Vmn2r81	UTSW	10	79,083,628 (GRCm39)	start codon destroyed	probably null	0.71
R1944:Vmn2r81	UTSW	10	79,129,571 (GRCm39)	missense	probably damaging	1.00
R2102:Vmn2r81	UTSW	10	79,129,334 (GRCm39)	missense	probably damaging	0.97
R2190:Vmn2r81	UTSW	10	79,104,085 (GRCm39)	missense	possibly damaging	0.67
R2392:Vmn2r81	UTSW	10	79,110,516 (GRCm39)	missense	probably damaging	1.00
R3770:Vmn2r81	UTSW	10	79,106,434 (GRCm39)	missense	probably damaging	1.00
R4551:Vmn2r81	UTSW	10	79,104,241 (GRCm39)	missense	possibly damaging	0.95
R4629:Vmn2r81	UTSW	10	79,103,276 (GRCm39)	missense	probably damaging	0.99
R4691:Vmn2r81	UTSW	10	79,129,211 (GRCm39)	nonsense	probably null
R4829:Vmn2r81	UTSW	10	79,083,635 (GRCm39)	missense	possibly damaging	0.48
R4914:Vmn2r81	UTSW	10	79,106,357 (GRCm39)	missense	probably null	1.00
R4996:Vmn2r81	UTSW	10	79,129,247 (GRCm39)	missense	probably benign	0.04
R5242:Vmn2r81	UTSW	10	79,129,309 (GRCm39)	nonsense	probably null
R5253:Vmn2r81	UTSW	10	79,083,820 (GRCm39)	missense	probably benign	0.00
R5925:Vmn2r81	UTSW	10	79,083,637 (GRCm39)	missense	probably damaging	1.00
R6169:Vmn2r81	UTSW	10	79,104,382 (GRCm39)	missense	probably benign
R6270:Vmn2r81	UTSW	10	79,129,649 (GRCm39)	missense	probably benign
R6525:Vmn2r81	UTSW	10	79,129,560 (GRCm39)	missense	probably benign	0.11
R6813:Vmn2r81	UTSW	10	79,104,439 (GRCm39)	missense	probably benign	0.00
R6886:Vmn2r81	UTSW	10	79,103,988 (GRCm39)	missense	possibly damaging	0.90
R7200:Vmn2r81	UTSW	10	79,106,570 (GRCm39)	critical splice donor site	probably null
R7350:Vmn2r81	UTSW	10	79,104,219 (GRCm39)	missense	probably benign	0.03
R7504:Vmn2r81	UTSW	10	79,104,166 (GRCm39)	missense	probably benign
R7832:Vmn2r81	UTSW	10	79,129,664 (GRCm39)	missense	probably damaging	1.00
R8130:Vmn2r81	UTSW	10	79,110,538 (GRCm39)	missense	possibly damaging	0.95
R8362:Vmn2r81	UTSW	10	79,103,283 (GRCm39)	missense	probably benign	0.17
R8407:Vmn2r81	UTSW	10	79,104,028 (GRCm39)	missense	possibly damaging	0.92
R8540:Vmn2r81	UTSW	10	79,129,065 (GRCm39)	missense	probably damaging	0.99
R8558:Vmn2r81	UTSW	10	79,106,467 (GRCm39)	missense	possibly damaging	0.94
R8700:Vmn2r81	UTSW	10	79,129,517 (GRCm39)	missense	probably damaging	1.00
R8779:Vmn2r81	UTSW	10	79,103,218 (GRCm39)	missense	possibly damaging	0.78
R8987:Vmn2r81	UTSW	10	79,129,704 (GRCm39)	missense	probably damaging	1.00
R9001:Vmn2r81	UTSW	10	79,104,104 (GRCm39)	missense	possibly damaging	0.73
R9055:Vmn2r81	UTSW	10	79,110,441 (GRCm39)	missense	probably benign
R9145:Vmn2r81	UTSW	10	79,104,028 (GRCm39)	missense	possibly damaging	0.92
R9151:Vmn2r81	UTSW	10	79,103,905 (GRCm39)	missense
R9253:Vmn2r81	UTSW	10	79,129,582 (GRCm39)	missense	probably damaging	1.00
R9255:Vmn2r81	UTSW	10	79,103,166 (GRCm39)	missense	possibly damaging	0.77
R9370:Vmn2r81	UTSW	10	79,104,424 (GRCm39)	missense	probably damaging	1.00
R9588:Vmn2r81	UTSW	10	79,129,205 (GRCm39)	missense	possibly damaging	0.46
X0067:Vmn2r81	UTSW	10	79,128,877 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TTCTTATCCAGGAGACTGCCCGAC -3'
(R):5'- AGCATGGCATTCTAGGGAATCAAGC -3'

Sequencing Primer
(F):5'- GACTGCCCGACAGATTTAATG -3'
(R):5'- GCATTCTAGGGAATCAAGCTACAG -3'

Posted On

2014-03-14