Mutagenetix > Incidental Mutation

Incidental Mutation 'R1388:Vmn2r12'

162487

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

039450-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R1388 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109233715-109245730 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 109240840 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Serine at position 91 (Y91S)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

possibly damaging
Transcript: ENSMUST00000095922
AA Change: Y91S

PolyPhen 2 Score 0.564 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: Y91S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 94.9%
20x: 88.1%

Validation Efficiency

98% (47/48)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot3	T	G	12: 84,105,761 (GRCm39)	H409Q	possibly damaging	Het
Adgra1	T	C	7: 139,453,919 (GRCm39)	V152A	probably damaging	Het
Arhgef6	T	C	X: 56,383,922 (GRCm39)	M5V	probably benign	Het
Atxn7l3	C	G	11: 102,183,261 (GRCm39)		probably benign	Het
Ccdc174	C	A	6: 91,858,225 (GRCm39)		probably null	Het
Ccdc38	A	T	10: 93,417,702 (GRCm39)		probably benign	Het
Clca4b	A	T	3: 144,622,415 (GRCm39)	V550D	probably benign	Het
Dab2ip	C	T	2: 35,611,268 (GRCm39)		probably benign	Het
Gm17661	GA	GAA	2: 90,917,709 (GRCm38)		noncoding transcript	Het
Gm2959	A	T	14: 42,235,660 (GRCm39)		noncoding transcript	Het
Gmnc	A	G	16: 26,782,662 (GRCm39)	L80P	probably damaging	Het
Gtf2ird1	T	C	5: 134,424,564 (GRCm39)	D394G	probably damaging	Het
Heatr1	T	C	13: 12,432,328 (GRCm39)		probably benign	Het
Il1a	T	C	2: 129,148,501 (GRCm39)	S70G	possibly damaging	Het
Kctd19	T	C	8: 106,118,683 (GRCm39)	S293G	probably null	Het
Klra4	T	C	6: 130,039,198 (GRCm39)		probably benign	Het
Kplce	G	T	3: 92,776,356 (GRCm39)	T109K	probably damaging	Het
Mr1	T	C	1: 155,008,249 (GRCm39)	E242G	probably damaging	Het
Mrnip	C	A	11: 50,087,772 (GRCm39)	A98E	probably benign	Het
Mybpc3	C	T	2: 90,953,219 (GRCm39)	P155S	probably benign	Het
Myh14	A	T	7: 44,314,546 (GRCm39)	Y126N	probably damaging	Het
Nrbp1	T	A	5: 31,403,157 (GRCm39)	I210N	probably damaging	Het
Nup58	A	T	14: 60,482,119 (GRCm39)		probably benign	Het
Or10g9	A	G	9: 39,911,948 (GRCm39)	S192P	probably damaging	Het
Or10j3	T	C	1: 173,031,445 (GRCm39)	V174A	probably benign	Het
Pnisr	T	C	4: 21,862,041 (GRCm39)	M243T	possibly damaging	Het
Ptprr	A	G	10: 116,109,657 (GRCm39)	S633G	probably benign	Het
Rasip1	T	A	7: 45,279,656 (GRCm39)	S300T	probably damaging	Het
Sbsn	A	T	7: 30,451,576 (GRCm39)	H197L	probably benign	Het
Shf	G	A	2: 122,199,163 (GRCm39)	P51S	probably damaging	Het
Sim1	T	A	10: 50,772,090 (GRCm39)	I33N	probably damaging	Het
Speg	A	G	1: 75,407,104 (GRCm39)	D2878G	probably damaging	Het
Taf2	T	A	15: 54,900,021 (GRCm39)	N864I	probably benign	Het
Tmem43	G	T	6: 91,455,785 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,542,135 (GRCm39)	E25290G	probably damaging	Het
Ush2a	A	G	1: 188,255,515 (GRCm39)		probably benign	Het
Usp53	T	C	3: 122,751,277 (GRCm39)	E260G	probably damaging	Het
Vmn2r59	A	T	7: 41,695,133 (GRCm39)	N426K	probably benign	Het
Whamm	C	A	7: 81,236,038 (GRCm39)	L414I	probably damaging	Het
Zfhx4	A	G	3: 5,466,447 (GRCm39)	T2227A	probably damaging	Het
Zfp866	C	T	8: 70,218,834 (GRCm39)	R262Q	probably benign	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109,245,541 (GRCm39)	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109,234,125 (GRCm39)	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109,239,716 (GRCm39)	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109,240,893 (GRCm39)	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109,240,025 (GRCm39)	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109,234,343 (GRCm39)	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109,233,858 (GRCm39)	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109,238,351 (GRCm39)	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109,239,936 (GRCm39)	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109,240,765 (GRCm39)	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109,239,755 (GRCm39)	nonsense	probably null
R0529:Vmn2r12	UTSW	5	109,240,714 (GRCm39)	missense	probably benign
R0715:Vmn2r12	UTSW	5	109,238,373 (GRCm39)	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109,234,281 (GRCm39)	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109,235,716 (GRCm39)	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109,239,763 (GRCm39)	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R1549:Vmn2r12	UTSW	5	109,240,696 (GRCm39)	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109,239,910 (GRCm39)	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109,239,594 (GRCm39)	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109,239,942 (GRCm39)	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109,239,340 (GRCm39)	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109,238,370 (GRCm39)	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109,239,412 (GRCm39)	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109,234,301 (GRCm39)	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109,234,379 (GRCm39)	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109,240,852 (GRCm39)	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109,239,544 (GRCm39)	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109,239,372 (GRCm39)	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109,238,261 (GRCm39)	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109,239,684 (GRCm39)	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109,234,483 (GRCm39)	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109,240,666 (GRCm39)	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109,239,670 (GRCm39)	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109,233,736 (GRCm39)	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109,240,763 (GRCm39)	missense	probably benign
R6162:Vmn2r12	UTSW	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109,233,866 (GRCm39)	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109,240,771 (GRCm39)	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109,245,655 (GRCm39)	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109,239,811 (GRCm39)	missense	possibly damaging	0.95
R7341:Vmn2r12	UTSW	5	109,234,113 (GRCm39)	missense	possibly damaging	0.74
R7383:Vmn2r12	UTSW	5	109,240,684 (GRCm39)	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109,239,615 (GRCm39)	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109,233,920 (GRCm39)	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109,235,829 (GRCm39)	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109,234,307 (GRCm39)	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109,239,747 (GRCm39)	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109,238,349 (GRCm39)	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109,234,074 (GRCm39)	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109,239,952 (GRCm39)	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109,234,522 (GRCm39)	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109,234,033 (GRCm39)	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109,233,885 (GRCm39)	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109,240,910 (GRCm39)	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109,234,203 (GRCm39)	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109,233,986 (GRCm39)	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109,239,823 (GRCm39)	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109,239,322 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109,240,646 (GRCm39)	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109,239,303 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACCCTTGGTGTCCCATGATGAATTG -3'
(R):5'- GCTACGCAGACTGCTCTTATGACC -3'

Sequencing Primer
(F):5'- TACAGATGTTTTCCATGATGGTCC -3'
(R):5'- TCATGGCAGCACTCTGAC -3'

Posted On

2014-03-17