Mutagenetix > Incidental Mutation

Incidental Mutation 'R1484:Hmcn2'

163281

Institutional Source

Beutler Lab

Gene Symbol

Hmcn2

Ensembl Gene

ENSMUSG00000055632

Gene Name

hemicentin 2

Synonyms

MMRRC Submission

039537-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1484 (G1)

Quality Score

214

Status

Validated

Chromosome

Chromosomal Location

31204427-31350750 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 31236507 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 350 (G350D)

Ref Sequence

ENSEMBL: ENSMUSP00000154649 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000113532] [ENSMUST00000226996]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000113532
AA Change: G350D

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000109160
Gene: ENSMUSG00000055632
AA Change: G350D

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
VWA	37	211	1.21e-1	SMART
Blast:IG_like	263	340	2e-38	BLAST
IG	434	515	7.36e-2	SMART
IGc2	530	595	1.91e-9	SMART
IGc2	621	685	4.81e-15	SMART
IGc2	711	773	1.09e-13	SMART
IGc2	799	866	2.72e-14	SMART
IGc2	894	959	1.95e-15	SMART
IGc2	985	1049	5e-13	SMART
IGc2	1082	1147	1.09e-13	SMART
low complexity region	1151	1169	N/A	INTRINSIC
IGc2	1173	1232	7.07e-13	SMART
IGc2	1260	1326	4.31e-17	SMART
IGc2	1354	1428	3e-16	SMART
IGc2	1456	1522	1.82e-15	SMART
IGc2	1550	1615	2.7e-18	SMART
IGc2	1644	1708	1.3e-11	SMART
IGc2	1736	1801	6.69e-14	SMART
IG	1826	1917	2.31e0	SMART
IGc2	1932	1997	4.62e-17	SMART
IGc2	2024	2091	3.25e-12	SMART
IGc2	2117	2182	1.28e-10	SMART
IGc2	2209	2276	3.76e-8	SMART
IGc2	2305	2370	2.6e-11	SMART
IGc2	2399	2464	1.32e-12	SMART
IGc2	2492	2557	2.06e-14	SMART
IGc2	2588	2653	3.9e-15	SMART
IGc2	2686	2751	2.64e-12	SMART
IGc2	2797	2862	9.05e-11	SMART
IGc2	2892	2957	4.7e-9	SMART
IGc2	2984	3049	1.44e-13	SMART
IGc2	3079	3144	9.33e-13	SMART
IGc2	3171	3236	3.79e-13	SMART
IGc2	3264	3331	1.85e-16	SMART
IGc2	3360	3425	9.61e-15	SMART
low complexity region	3433	3445	N/A	INTRINSIC
IGc2	3453	3514	5.83e-14	SMART
IGc2	3542	3600	1.76e-8	SMART
low complexity region	3613	3627	N/A	INTRINSIC
IGc2	3628	3693	5.2e-11	SMART
IGc2	3719	3784	2.64e-12	SMART
IGc2	3810	3877	3.35e-5	SMART
IGc2	3903	3968	3.73e-12	SMART
IGc2	3994	4058	4.39e-9	SMART
IGc2	4084	4149	1.79e-14	SMART
low complexity region	4157	4169	N/A	INTRINSIC
IGc2	4175	4238	9.33e-13	SMART
IGc2	4265	4329	7.22e-19	SMART
IGc2	4355	4419	1.59e-15	SMART
Pfam:G2F	4431	4613	1.7e-56	PFAM
EGF_CA	4668	4708	5.78e-11	SMART
EGF_CA	4709	4753	9.39e-11	SMART
EGF_CA	4754	4796	7.69e-7	SMART
EGF_CA	4797	4837	2.19e-11	SMART
EGF_CA	4904	4943	6.74e-12	SMART
EGF_like	4944	4989	1.87e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000226996
AA Change: G350D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.1219

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.4%

Validation Efficiency

97% (85/88)

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930584F24Rik	A	T	5: 26,684,776 (GRCm39)		noncoding transcript	Het
Acvr1	A	T	2: 58,369,901 (GRCm39)	V36E	probably damaging	Het
Aldh4a1	A	G	4: 139,370,758 (GRCm39)	I414V	probably benign	Het
Alox5	C	T	6: 116,431,128 (GRCm39)	C100Y	probably damaging	Het
Ano5	T	A	7: 51,216,068 (GRCm39)	D348E	probably damaging	Het
Arhgap30	G	A	1: 171,230,839 (GRCm39)	V199M	probably damaging	Het
Arl13b	T	A	16: 62,626,999 (GRCm39)	Q234L	probably benign	Het
Atxn1	C	A	13: 45,711,052 (GRCm39)	E627*	probably null	Het
Bend3	T	C	10: 43,386,197 (GRCm39)	F197L	probably benign	Het
Brca1	A	T	11: 101,420,638 (GRCm39)	V190E	possibly damaging	Het
Brpf1	T	C	6: 113,292,096 (GRCm39)	W381R	probably damaging	Het
Brwd1	A	C	16: 95,829,491 (GRCm39)		probably null	Het
C1s2	T	C	6: 124,602,604 (GRCm39)	I530V	possibly damaging	Het
C2cd3	C	T	7: 100,089,397 (GRCm39)	R1638W	probably damaging	Het
Capns1	T	A	7: 29,893,511 (GRCm39)		probably benign	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Cep126	G	A	9: 8,100,554 (GRCm39)	T660I	possibly damaging	Het
Cep295	A	C	9: 15,246,080 (GRCm39)	I744R	probably damaging	Het
Chd3	T	C	11: 69,250,725 (GRCm39)	E668G	probably benign	Het
Chek2	A	G	5: 110,996,553 (GRCm39)	T172A	probably damaging	Het
Col6a4	A	G	9: 105,890,501 (GRCm39)		probably null	Het
Coq3	G	A	4: 21,900,291 (GRCm39)	V173I	probably benign	Het
Cyp4x1	A	T	4: 114,970,098 (GRCm39)	I343N	probably damaging	Het
Dnah7b	T	A	1: 46,176,703 (GRCm39)	D774E	probably benign	Het
Dnai3	T	C	3: 145,802,996 (GRCm39)	D65G	probably benign	Het
Ecm1	G	A	3: 95,643,275 (GRCm39)	R342C	probably damaging	Het
Esrra	T	C	19: 6,890,197 (GRCm39)	Y209C	probably damaging	Het
Gpr149	A	T	3: 62,502,592 (GRCm39)	D421E	probably benign	Het
Gpr15	T	C	16: 58,538,937 (GRCm39)	N51D	probably damaging	Het
Gpr156	T	C	16: 37,812,558 (GRCm39)	V298A	probably damaging	Het
Ifih1	A	T	2: 62,440,902 (GRCm39)	N421K	probably benign	Het
Ilvbl	C	A	10: 78,412,564 (GRCm39)	T95K	probably damaging	Het
Itgb4	T	A	11: 115,890,625 (GRCm39)	D1104E	probably benign	Het
Katnip	C	A	7: 125,415,743 (GRCm39)		probably benign	Het
Lipf	A	T	19: 33,942,180 (GRCm39)	M37L	probably benign	Het
Lyst	T	A	13: 13,852,775 (GRCm39)	N2258K	probably benign	Het
Moxd1	A	G	10: 24,099,758 (GRCm39)	Y86C	probably damaging	Het
Muc5ac	T	C	7: 141,367,629 (GRCm39)		probably null	Het
Myo16	G	A	8: 10,610,145 (GRCm39)	R1162H	probably damaging	Het
Myo5c	A	T	9: 75,208,092 (GRCm39)	N1609Y	probably damaging	Het
Nbeal1	T	C	1: 60,240,098 (GRCm39)	F155L	probably damaging	Het
Nek4	A	T	14: 30,704,290 (GRCm39)	M602L	possibly damaging	Het
Nek9	A	G	12: 85,348,622 (GRCm39)	S971P	probably damaging	Het
Nfya	A	T	17: 48,700,570 (GRCm39)		probably benign	Het
Nrxn3	A	T	12: 89,221,547 (GRCm39)	N442I	probably damaging	Het
Nup42	A	C	5: 24,383,075 (GRCm39)	K200N	probably benign	Het
Or1e26	T	A	11: 73,480,187 (GRCm39)	I126L	possibly damaging	Het
Or4c111	G	A	2: 88,843,713 (GRCm39)	R232*	probably null	Het
Or7g32	G	A	9: 19,389,423 (GRCm39)	T38I	probably damaging	Het
Pcdh15	T	A	10: 74,126,833 (GRCm39)	I304N	probably damaging	Het
Pigo	G	C	4: 43,024,779 (GRCm39)	P107A	probably damaging	Het
Plce1	C	T	19: 38,693,783 (GRCm39)	Q769*	probably null	Het
Plin2	C	T	4: 86,575,481 (GRCm39)	R356H	probably benign	Het
Ppp1r9a	G	T	6: 5,113,712 (GRCm39)	E739*	probably null	Het
Ppp3cc	G	T	14: 70,478,397 (GRCm39)	N268K	probably damaging	Het
Prkag3	T	A	1: 74,779,919 (GRCm39)	D472V	probably damaging	Het
Ptch2	A	T	4: 116,968,046 (GRCm39)	D846V	probably damaging	Het
Rhob	A	T	12: 8,549,388 (GRCm39)	M82K	probably damaging	Het
Rps6kc1	T	A	1: 190,531,672 (GRCm39)	R777W	possibly damaging	Het
Sap130	T	A	18: 31,844,380 (GRCm39)	V850E	probably damaging	Het
Sema3a	T	G	5: 13,523,407 (GRCm39)	N125K	probably damaging	Het
Sema5a	A	G	15: 32,460,431 (GRCm39)	D64G	probably damaging	Het
Sgo2b	T	A	8: 64,384,507 (GRCm39)	D163V	possibly damaging	Het
Slc15a1	A	T	14: 121,728,651 (GRCm39)	Y31*	probably null	Het
Smchd1	A	T	17: 71,685,252 (GRCm39)	M1392K	probably benign	Het
Sobp	T	A	10: 43,036,827 (GRCm39)	N37I	probably damaging	Het
Spock3	G	T	8: 63,673,739 (GRCm39)	C142F	probably damaging	Het
Stx6	A	C	1: 155,053,650 (GRCm39)	S86R	probably benign	Het
Sult2a4	C	A	7: 13,643,726 (GRCm39)	M280I	probably benign	Het
Synm	A	T	7: 67,386,080 (GRCm39)	D527E	probably damaging	Het
Tax1bp1	C	T	6: 52,710,305 (GRCm39)	R195W	probably damaging	Het
Themis2	A	T	4: 132,519,796 (GRCm39)	N76K	possibly damaging	Het
Tmem8b	A	G	4: 43,690,234 (GRCm39)	T890A	probably benign	Het
Traf7	T	A	17: 24,730,785 (GRCm39)	H366L	possibly damaging	Het
Trim30c	A	T	7: 104,032,459 (GRCm39)	V289D	probably benign	Het
Tsr1	T	A	11: 74,792,914 (GRCm39)	D407E	probably damaging	Het
Ubap2	G	T	4: 41,235,593 (GRCm39)	A33E	probably damaging	Het
Unc13d	C	A	11: 115,964,701 (GRCm39)	R255L	possibly damaging	Het
Ush2a	G	T	1: 188,542,534 (GRCm39)	G3367*	probably null	Het
Vmn1r229	T	C	17: 21,034,791 (GRCm39)	L12P	probably damaging	Het
Vmn2r27	C	A	6: 124,177,474 (GRCm39)	G510V	probably damaging	Het
Vps4b	T	C	1: 106,707,712 (GRCm39)	E257G	probably damaging	Het
Vps72	T	C	3: 95,026,462 (GRCm39)	S136P	probably damaging	Het
Wdr36	T	C	18: 32,976,938 (GRCm39)	I181T	possibly damaging	Het
Wfikkn1	C	T	17: 26,096,765 (GRCm39)	A520T	probably benign	Het

Other mutations in Hmcn2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Hmcn2	APN	2	31,233,108 (GRCm39)	missense	probably damaging	1.00
IGL00966:Hmcn2	APN	2	31,319,006 (GRCm39)	missense	probably damaging	0.97
IGL00973:Hmcn2	APN	2	31,273,833 (GRCm39)	intron	probably benign
IGL01364:Hmcn2	APN	2	31,251,826 (GRCm39)	nonsense	probably null
IGL01486:Hmcn2	APN	2	31,226,633 (GRCm39)	missense	probably damaging	1.00
IGL01530:Hmcn2	APN	2	31,244,276 (GRCm39)	missense	possibly damaging	0.85
IGL01550:Hmcn2	APN	2	31,314,264 (GRCm39)	missense	possibly damaging	0.84
IGL01710:Hmcn2	APN	2	31,233,114 (GRCm39)	missense	probably damaging	1.00
IGL01764:Hmcn2	APN	2	31,295,642 (GRCm39)	missense	possibly damaging	0.93
IGL01924:Hmcn2	APN	2	31,288,929 (GRCm39)	missense	probably benign	0.00
IGL02003:Hmcn2	APN	2	31,318,994 (GRCm39)	missense	possibly damaging	0.90
IGL02117:Hmcn2	APN	2	31,347,185 (GRCm39)	missense	possibly damaging	0.75
IGL02205:Hmcn2	APN	2	31,290,139 (GRCm39)	missense	probably damaging	1.00
IGL02273:Hmcn2	APN	2	31,314,389 (GRCm39)	missense	probably benign	0.06
IGL02313:Hmcn2	APN	2	31,343,617 (GRCm39)	missense	possibly damaging	0.68
IGL02326:Hmcn2	APN	2	31,340,964 (GRCm39)	missense	probably damaging	0.97
IGL02486:Hmcn2	APN	2	31,310,107 (GRCm39)	missense	probably damaging	0.98
IGL02551:Hmcn2	APN	2	31,344,823 (GRCm39)	missense	possibly damaging	0.83
IGL02695:Hmcn2	APN	2	31,298,985 (GRCm39)	missense	possibly damaging	0.87
IGL02725:Hmcn2	APN	2	31,295,540 (GRCm39)	missense	probably damaging	1.00
IGL02792:Hmcn2	APN	2	31,236,602 (GRCm39)	missense	probably damaging	1.00
IGL02882:Hmcn2	APN	2	31,303,379 (GRCm39)	nonsense	probably null
IGL03003:Hmcn2	APN	2	31,323,498 (GRCm39)	missense	probably damaging	0.98
IGL03067:Hmcn2	APN	2	31,236,642 (GRCm39)	missense	probably damaging	1.00
IGL03137:Hmcn2	APN	2	31,252,242 (GRCm39)	missense	probably damaging	0.98
IGL03220:Hmcn2	APN	2	31,236,633 (GRCm39)	missense	possibly damaging	0.94
IGL03411:Hmcn2	APN	2	31,236,649 (GRCm39)	missense	possibly damaging	0.83
PIT4544001:Hmcn2	UTSW	2	31,318,262 (GRCm39)	missense	probably damaging	0.98
R0044:Hmcn2	UTSW	2	31,302,520 (GRCm39)	missense	probably damaging	0.98
R0044:Hmcn2	UTSW	2	31,302,520 (GRCm39)	missense	probably damaging	0.98
R0048:Hmcn2	UTSW	2	31,318,249 (GRCm39)	missense	possibly damaging	0.92
R0048:Hmcn2	UTSW	2	31,318,249 (GRCm39)	missense	possibly damaging	0.92
R0078:Hmcn2	UTSW	2	31,278,356 (GRCm39)	missense	probably damaging	1.00
R0090:Hmcn2	UTSW	2	31,316,210 (GRCm39)	missense	probably damaging	1.00
R0173:Hmcn2	UTSW	2	31,328,343 (GRCm39)	critical splice donor site	probably null
R0257:Hmcn2	UTSW	2	31,259,176 (GRCm39)	splice site	probably benign
R0266:Hmcn2	UTSW	2	31,335,365 (GRCm39)	splice site	probably benign
R0266:Hmcn2	UTSW	2	31,284,839 (GRCm39)	missense	probably benign	0.03
R0326:Hmcn2	UTSW	2	31,313,237 (GRCm39)	nonsense	probably null
R0366:Hmcn2	UTSW	2	31,314,218 (GRCm39)	missense	possibly damaging	0.88
R0400:Hmcn2	UTSW	2	31,290,141 (GRCm39)	missense	probably damaging	0.98
R0412:Hmcn2	UTSW	2	31,278,259 (GRCm39)	missense	probably damaging	0.98
R0436:Hmcn2	UTSW	2	31,295,624 (GRCm39)	missense	probably damaging	1.00
R0457:Hmcn2	UTSW	2	31,305,296 (GRCm39)	critical splice donor site	probably null
R0487:Hmcn2	UTSW	2	31,276,689 (GRCm39)	missense	possibly damaging	0.60
R0568:Hmcn2	UTSW	2	31,305,248 (GRCm39)	missense	probably benign	0.02
R0755:Hmcn2	UTSW	2	31,343,172 (GRCm39)	missense	probably damaging	0.99
R0811:Hmcn2	UTSW	2	31,310,383 (GRCm39)	missense	probably damaging	0.99
R0812:Hmcn2	UTSW	2	31,310,383 (GRCm39)	missense	probably damaging	0.99
R0964:Hmcn2	UTSW	2	31,281,523 (GRCm39)	missense	probably benign	0.23
R0988:Hmcn2	UTSW	2	31,225,463 (GRCm39)	missense	probably damaging	1.00
R1509:Hmcn2	UTSW	2	31,204,491 (GRCm39)	missense	possibly damaging	0.86
R1535:Hmcn2	UTSW	2	31,310,419 (GRCm39)	missense	possibly damaging	0.91
R1574:Hmcn2	UTSW	2	31,294,899 (GRCm39)	missense	probably damaging	0.97
R1574:Hmcn2	UTSW	2	31,294,899 (GRCm39)	missense	probably damaging	0.97
R1600:Hmcn2	UTSW	2	31,320,799 (GRCm39)	missense	probably damaging	0.98
R1623:Hmcn2	UTSW	2	31,348,051 (GRCm39)	missense	possibly damaging	0.84
R1692:Hmcn2	UTSW	2	31,340,856 (GRCm39)	missense	possibly damaging	0.47
R1719:Hmcn2	UTSW	2	31,244,733 (GRCm39)	missense	probably damaging	1.00
R1747:Hmcn2	UTSW	2	31,347,997 (GRCm39)	missense	probably benign	0.00
R1756:Hmcn2	UTSW	2	31,286,132 (GRCm39)	missense	probably damaging	0.99
R1763:Hmcn2	UTSW	2	31,204,602 (GRCm39)	missense	probably damaging	1.00
R1815:Hmcn2	UTSW	2	31,283,055 (GRCm39)	missense	probably damaging	0.97
R1822:Hmcn2	UTSW	2	31,273,704 (GRCm39)	missense	probably damaging	0.99
R1858:Hmcn2	UTSW	2	31,305,295 (GRCm39)	critical splice donor site	probably null
R1895:Hmcn2	UTSW	2	31,295,647 (GRCm39)	missense	probably damaging	0.99
R1908:Hmcn2	UTSW	2	31,301,922 (GRCm39)	critical splice donor site	probably null
R1946:Hmcn2	UTSW	2	31,295,647 (GRCm39)	missense	probably damaging	0.99
R1966:Hmcn2	UTSW	2	31,279,341 (GRCm39)	missense	probably damaging	0.99
R2007:Hmcn2	UTSW	2	31,328,267 (GRCm39)	missense	possibly damaging	0.91
R2050:Hmcn2	UTSW	2	31,225,448 (GRCm39)	missense	probably damaging	1.00
R2055:Hmcn2	UTSW	2	31,268,294 (GRCm39)	missense	probably benign	0.33
R2097:Hmcn2	UTSW	2	31,270,431 (GRCm39)	missense	probably damaging	1.00
R2145:Hmcn2	UTSW	2	31,223,943 (GRCm39)	splice site	probably benign
R2155:Hmcn2	UTSW	2	31,350,361 (GRCm39)	missense	possibly damaging	0.68
R2170:Hmcn2	UTSW	2	31,270,293 (GRCm39)	missense	probably benign	0.08
R2188:Hmcn2	UTSW	2	31,309,947 (GRCm39)	missense	probably benign	0.14
R2208:Hmcn2	UTSW	2	31,270,309 (GRCm39)	missense	probably damaging	1.00
R2217:Hmcn2	UTSW	2	31,240,586 (GRCm39)	missense	probably benign	0.02
R2407:Hmcn2	UTSW	2	31,225,424 (GRCm39)	critical splice acceptor site	probably null
R2764:Hmcn2	UTSW	2	31,278,310 (GRCm39)	missense	probably damaging	0.98
R2913:Hmcn2	UTSW	2	31,350,222 (GRCm39)	missense	possibly damaging	0.68
R2986:Hmcn2	UTSW	2	31,251,010 (GRCm39)	missense	probably damaging	1.00
R3157:Hmcn2	UTSW	2	31,290,267 (GRCm39)	missense	probably damaging	0.99
R3406:Hmcn2	UTSW	2	31,323,284 (GRCm39)	splice site	probably benign
R3429:Hmcn2	UTSW	2	31,299,156 (GRCm39)	missense	possibly damaging	0.87
R3737:Hmcn2	UTSW	2	31,226,624 (GRCm39)	nonsense	probably null
R3739:Hmcn2	UTSW	2	31,226,624 (GRCm39)	nonsense	probably null
R3771:Hmcn2	UTSW	2	31,250,908 (GRCm39)	missense	probably damaging	0.99
R3772:Hmcn2	UTSW	2	31,250,908 (GRCm39)	missense	probably damaging	0.99
R3773:Hmcn2	UTSW	2	31,250,908 (GRCm39)	missense	probably damaging	0.99
R3804:Hmcn2	UTSW	2	31,242,897 (GRCm39)	splice site	probably null
R3837:Hmcn2	UTSW	2	31,303,419 (GRCm39)	missense	probably damaging	0.99
R3838:Hmcn2	UTSW	2	31,303,419 (GRCm39)	missense	probably damaging	0.99
R3846:Hmcn2	UTSW	2	31,320,362 (GRCm39)	missense	possibly damaging	0.51
R3925:Hmcn2	UTSW	2	31,343,169 (GRCm39)	missense	probably benign	0.00
R3934:Hmcn2	UTSW	2	31,270,496 (GRCm39)	critical splice donor site	probably null
R3946:Hmcn2	UTSW	2	31,272,406 (GRCm39)	missense	possibly damaging	0.91
R4035:Hmcn2	UTSW	2	31,226,624 (GRCm39)	nonsense	probably null
R4057:Hmcn2	UTSW	2	31,290,250 (GRCm39)	missense	probably damaging	1.00
R4583:Hmcn2	UTSW	2	31,303,277 (GRCm39)	missense	possibly damaging	0.84
R4623:Hmcn2	UTSW	2	31,286,722 (GRCm39)	missense	probably damaging	1.00
R4647:Hmcn2	UTSW	2	31,289,031 (GRCm39)	missense	possibly damaging	0.82
R4668:Hmcn2	UTSW	2	31,325,804 (GRCm39)	missense	probably benign	0.40
R4669:Hmcn2	UTSW	2	31,325,804 (GRCm39)	missense	probably benign	0.40
R4687:Hmcn2	UTSW	2	31,328,297 (GRCm39)	missense	probably benign	0.14
R4735:Hmcn2	UTSW	2	31,273,787 (GRCm39)	missense	probably benign	0.06
R4772:Hmcn2	UTSW	2	31,335,326 (GRCm39)	missense	probably benign	0.02
R4866:Hmcn2	UTSW	2	31,279,403 (GRCm39)	missense	possibly damaging	0.88
R4916:Hmcn2	UTSW	2	31,250,992 (GRCm39)	missense	probably damaging	0.98
R4943:Hmcn2	UTSW	2	31,225,504 (GRCm39)	missense	probably damaging	1.00
R4967:Hmcn2	UTSW	2	31,244,176 (GRCm39)	critical splice acceptor site	probably null
R4973:Hmcn2	UTSW	2	31,234,108 (GRCm39)	missense	probably benign	0.15
R4975:Hmcn2	UTSW	2	31,283,037 (GRCm39)	missense	possibly damaging	0.88
R4994:Hmcn2	UTSW	2	31,348,067 (GRCm39)	critical splice donor site	probably null
R4997:Hmcn2	UTSW	2	31,291,720 (GRCm39)	missense	probably damaging	1.00
R5045:Hmcn2	UTSW	2	31,299,093 (GRCm39)	missense	probably damaging	1.00
R5117:Hmcn2	UTSW	2	31,348,061 (GRCm39)	missense	possibly damaging	0.95
R5151:Hmcn2	UTSW	2	31,279,455 (GRCm39)	missense	probably null
R5232:Hmcn2	UTSW	2	31,347,760 (GRCm39)	missense	probably damaging	0.99
R5237:Hmcn2	UTSW	2	31,304,728 (GRCm39)	missense	probably benign	0.01
R5288:Hmcn2	UTSW	2	31,350,333 (GRCm39)	missense	probably benign	0.11
R5375:Hmcn2	UTSW	2	31,320,453 (GRCm39)	missense	possibly damaging	0.92
R5379:Hmcn2	UTSW	2	31,299,023 (GRCm39)	missense	probably damaging	0.99
R5385:Hmcn2	UTSW	2	31,350,333 (GRCm39)	missense	probably benign	0.11
R5412:Hmcn2	UTSW	2	31,236,629 (GRCm39)	missense	possibly damaging	0.77
R5426:Hmcn2	UTSW	2	31,226,556 (GRCm39)	missense	possibly damaging	0.95
R5434:Hmcn2	UTSW	2	31,310,375 (GRCm39)	missense	probably damaging	1.00
R5441:Hmcn2	UTSW	2	31,296,428 (GRCm39)	missense	possibly damaging	0.82
R5484:Hmcn2	UTSW	2	31,283,066 (GRCm39)	nonsense	probably null
R5492:Hmcn2	UTSW	2	31,310,318 (GRCm39)	missense	probably benign	0.03
R5572:Hmcn2	UTSW	2	31,304,538 (GRCm39)	critical splice acceptor site	probably null
R5572:Hmcn2	UTSW	2	31,304,537 (GRCm39)	critical splice acceptor site	probably null
R5591:Hmcn2	UTSW	2	31,234,059 (GRCm39)	missense	probably damaging	1.00
R5614:Hmcn2	UTSW	2	31,318,315 (GRCm39)	missense	probably damaging	0.99
R5634:Hmcn2	UTSW	2	31,223,893 (GRCm39)	missense	probably damaging	1.00
R5645:Hmcn2	UTSW	2	31,310,824 (GRCm39)	missense	possibly damaging	0.92
R5716:Hmcn2	UTSW	2	31,348,750 (GRCm39)	missense	possibly damaging	0.68
R5716:Hmcn2	UTSW	2	31,226,579 (GRCm39)	missense	probably damaging	1.00
R5725:Hmcn2	UTSW	2	31,273,827 (GRCm39)	critical splice donor site	probably null
R5760:Hmcn2	UTSW	2	31,304,580 (GRCm39)	missense	possibly damaging	0.91
R5774:Hmcn2	UTSW	2	31,299,147 (GRCm39)	missense	possibly damaging	0.94
R5838:Hmcn2	UTSW	2	31,347,819 (GRCm39)	missense	probably damaging	0.99
R5899:Hmcn2	UTSW	2	31,244,685 (GRCm39)	missense	possibly damaging	0.93
R5916:Hmcn2	UTSW	2	31,286,151 (GRCm39)	missense	probably damaging	1.00
R5973:Hmcn2	UTSW	2	31,310,335 (GRCm39)	missense	probably damaging	0.99
R6002:Hmcn2	UTSW	2	31,310,321 (GRCm39)	missense	probably damaging	0.99
R6018:Hmcn2	UTSW	2	31,260,804 (GRCm39)	missense	probably benign	0.13
R6063:Hmcn2	UTSW	2	31,324,725 (GRCm39)	missense	probably benign	0.06
R6161:Hmcn2	UTSW	2	31,246,266 (GRCm39)	missense	probably benign
R6166:Hmcn2	UTSW	2	31,259,274 (GRCm39)	missense	probably damaging	1.00
R6177:Hmcn2	UTSW	2	31,310,118 (GRCm39)	nonsense	probably null
R6191:Hmcn2	UTSW	2	31,348,758 (GRCm39)	missense	probably damaging	0.99
R6195:Hmcn2	UTSW	2	31,274,127 (GRCm39)	missense	probably damaging	0.96
R6273:Hmcn2	UTSW	2	31,301,846 (GRCm39)	missense	probably damaging	0.99
R6293:Hmcn2	UTSW	2	31,225,463 (GRCm39)	missense	probably damaging	1.00
R6349:Hmcn2	UTSW	2	31,278,385 (GRCm39)	missense	probably damaging	1.00
R6395:Hmcn2	UTSW	2	31,259,269 (GRCm39)	missense	probably damaging	1.00
R6448:Hmcn2	UTSW	2	31,310,832 (GRCm39)	missense	probably benign	0.02
R6450:Hmcn2	UTSW	2	31,251,812 (GRCm39)	missense	probably benign	0.11
R6479:Hmcn2	UTSW	2	31,315,480 (GRCm39)	missense	probably damaging	0.99
R6502:Hmcn2	UTSW	2	31,272,490 (GRCm39)	missense	probably damaging	0.99
R6511:Hmcn2	UTSW	2	31,246,354 (GRCm39)	missense	possibly damaging	0.79
R6537:Hmcn2	UTSW	2	31,305,280 (GRCm39)	missense	probably benign	0.00
R6880:Hmcn2	UTSW	2	31,233,068 (GRCm39)	missense	probably damaging	1.00
R6924:Hmcn2	UTSW	2	31,240,517 (GRCm39)	splice site	probably null
R6971:Hmcn2	UTSW	2	31,322,333 (GRCm39)	missense	probably benign	0.02
R7057:Hmcn2	UTSW	2	31,312,661 (GRCm39)	missense	probably damaging	0.99
R7141:Hmcn2	UTSW	2	31,250,908 (GRCm39)	missense	probably benign	0.17
R7268:Hmcn2	UTSW	2	31,347,978 (GRCm39)	missense	possibly damaging	0.48
R7307:Hmcn2	UTSW	2	31,233,093 (GRCm39)	missense	probably damaging	0.96
R7322:Hmcn2	UTSW	2	31,349,093 (GRCm39)	missense	probably damaging	0.99
R7334:Hmcn2	UTSW	2	31,343,147 (GRCm39)	missense	possibly damaging	0.82
R7334:Hmcn2	UTSW	2	31,325,806 (GRCm39)	missense	probably damaging	0.98
R7335:Hmcn2	UTSW	2	31,282,169 (GRCm39)	missense	possibly damaging	0.88
R7358:Hmcn2	UTSW	2	31,306,824 (GRCm39)	missense	probably damaging	1.00
R7359:Hmcn2	UTSW	2	31,278,395 (GRCm39)	missense	probably benign	0.13
R7488:Hmcn2	UTSW	2	31,310,842 (GRCm39)	missense	probably damaging	1.00
R7498:Hmcn2	UTSW	2	31,273,487 (GRCm39)	splice site	probably null
R7560:Hmcn2	UTSW	2	31,347,185 (GRCm39)	missense	probably benign
R7566:Hmcn2	UTSW	2	31,344,869 (GRCm39)	missense	probably damaging	0.96
R7570:Hmcn2	UTSW	2	31,313,923 (GRCm39)	missense	probably benign
R7574:Hmcn2	UTSW	2	31,345,531 (GRCm39)	missense	possibly damaging	0.68
R7599:Hmcn2	UTSW	2	31,246,298 (GRCm39)	missense	possibly damaging	0.93
R7654:Hmcn2	UTSW	2	31,236,581 (GRCm39)	missense	probably benign	0.00
R7662:Hmcn2	UTSW	2	31,272,357 (GRCm39)	missense	probably benign	0.01
R7666:Hmcn2	UTSW	2	31,270,245 (GRCm39)	missense	probably damaging	1.00
R7698:Hmcn2	UTSW	2	31,313,165 (GRCm39)	missense	probably damaging	0.98
R7722:Hmcn2	UTSW	2	31,272,512 (GRCm39)	nonsense	probably null
R7739:Hmcn2	UTSW	2	31,348,038 (GRCm39)	missense	possibly damaging	0.48
R7749:Hmcn2	UTSW	2	31,343,045 (GRCm39)	splice site	probably null
R7828:Hmcn2	UTSW	2	31,295,887 (GRCm39)	missense	possibly damaging	0.95
R7912:Hmcn2	UTSW	2	31,310,311 (GRCm39)	missense	probably benign	0.00
R7978:Hmcn2	UTSW	2	31,279,359 (GRCm39)	missense	probably benign	0.40
R8075:Hmcn2	UTSW	2	31,279,403 (GRCm39)	missense	possibly damaging	0.88
R8088:Hmcn2	UTSW	2	31,316,915 (GRCm39)	nonsense	probably null
R8101:Hmcn2	UTSW	2	31,240,082 (GRCm39)	missense	probably benign	0.08
R8124:Hmcn2	UTSW	2	31,290,136 (GRCm39)	missense	probably benign	0.01
R8145:Hmcn2	UTSW	2	31,313,117 (GRCm39)	missense	probably damaging	1.00
R8230:Hmcn2	UTSW	2	31,234,485 (GRCm39)	missense	possibly damaging	0.91
R8267:Hmcn2	UTSW	2	31,349,191 (GRCm39)	missense	probably benign
R8277:Hmcn2	UTSW	2	31,259,189 (GRCm39)	missense	probably benign	0.16
R8307:Hmcn2	UTSW	2	31,286,127 (GRCm39)	missense	probably damaging	0.99
R8353:Hmcn2	UTSW	2	31,275,353 (GRCm39)	splice site	probably null
R8415:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8416:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8437:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8438:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8440:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8442:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8497:Hmcn2	UTSW	2	31,313,357 (GRCm39)	missense	possibly damaging	0.92
R8520:Hmcn2	UTSW	2	31,244,726 (GRCm39)	missense	probably damaging	1.00
R8530:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8537:Hmcn2	UTSW	2	31,281,088 (GRCm39)	missense	probably benign	0.15
R8550:Hmcn2	UTSW	2	31,240,654 (GRCm39)	critical splice donor site	probably null
R8721:Hmcn2	UTSW	2	31,315,189 (GRCm39)	missense	probably damaging	1.00
R8795:Hmcn2	UTSW	2	31,315,393 (GRCm39)	missense	probably benign	0.01
R8802:Hmcn2	UTSW	2	31,301,288 (GRCm39)	missense	probably damaging	0.97
R8804:Hmcn2	UTSW	2	31,315,393 (GRCm39)	missense	probably benign	0.01
R8805:Hmcn2	UTSW	2	31,315,393 (GRCm39)	missense	probably benign	0.01
R8904:Hmcn2	UTSW	2	31,323,404 (GRCm39)	missense	possibly damaging	0.92
R8937:Hmcn2	UTSW	2	31,204,427 (GRCm39)	start codon destroyed	probably benign	0.01
R8947:Hmcn2	UTSW	2	31,278,220 (GRCm39)	missense	probably damaging	0.99
R8948:Hmcn2	UTSW	2	31,244,741 (GRCm39)	missense	probably damaging	1.00
R8950:Hmcn2	UTSW	2	31,244,741 (GRCm39)	missense	probably damaging	1.00
R8959:Hmcn2	UTSW	2	31,282,159 (GRCm39)	missense	probably damaging	1.00
R9025:Hmcn2	UTSW	2	31,347,967 (GRCm39)	missense	possibly damaging	0.56
R9039:Hmcn2	UTSW	2	31,244,646 (GRCm39)	missense	probably damaging	0.97
R9068:Hmcn2	UTSW	2	31,303,685 (GRCm39)	missense	probably benign	0.01
R9161:Hmcn2	UTSW	2	31,242,758 (GRCm39)	missense	probably benign	0.02
R9178:Hmcn2	UTSW	2	31,281,521 (GRCm39)	missense	possibly damaging	0.77
R9204:Hmcn2	UTSW	2	31,278,377 (GRCm39)	missense	probably damaging	0.98
R9317:Hmcn2	UTSW	2	31,350,328 (GRCm39)	missense	possibly damaging	0.91
R9341:Hmcn2	UTSW	2	31,279,359 (GRCm39)	missense	probably benign	0.40
R9343:Hmcn2	UTSW	2	31,279,359 (GRCm39)	missense	probably benign	0.40
R9355:Hmcn2	UTSW	2	31,328,302 (GRCm39)	missense	probably benign	0.18
R9371:Hmcn2	UTSW	2	31,301,917 (GRCm39)	missense	probably damaging	1.00
R9450:Hmcn2	UTSW	2	31,316,845 (GRCm39)	missense	probably damaging	1.00
R9477:Hmcn2	UTSW	2	31,286,031 (GRCm39)	critical splice acceptor site	probably null
R9483:Hmcn2	UTSW	2	31,320,375 (GRCm39)	missense
R9536:Hmcn2	UTSW	2	31,335,130 (GRCm39)	missense	possibly damaging	0.86
R9580:Hmcn2	UTSW	2	31,294,875 (GRCm39)	missense	probably benign	0.16
R9593:Hmcn2	UTSW	2	31,244,742 (GRCm39)	missense	probably damaging	0.99
R9649:Hmcn2	UTSW	2	31,292,450 (GRCm39)	missense	possibly damaging	0.95
R9706:Hmcn2	UTSW	2	31,305,279 (GRCm39)	missense	probably benign	0.00
X0066:Hmcn2	UTSW	2	31,344,823 (GRCm39)	missense	possibly damaging	0.83
X0067:Hmcn2	UTSW	2	31,295,879 (GRCm39)	missense	possibly damaging	0.82
Z1088:Hmcn2	UTSW	2	31,349,076 (GRCm39)	splice site	probably null
Z1088:Hmcn2	UTSW	2	31,271,079 (GRCm39)	missense	probably benign	0.01
Z1176:Hmcn2	UTSW	2	31,319,103 (GRCm39)	missense	probably damaging	0.97
Z1176:Hmcn2	UTSW	2	31,315,428 (GRCm39)	missense	probably damaging	1.00
Z1176:Hmcn2	UTSW	2	31,234,041 (GRCm39)	missense	possibly damaging	0.95
Z1177:Hmcn2	UTSW	2	31,316,836 (GRCm39)	missense	probably damaging	0.99
Z1177:Hmcn2	UTSW	2	31,234,518 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCGGAGCAGATCACAGCCAAC -3'
(R):5'- TGAGATAAAAGCGTTCCTTCGGCAC -3'

Sequencing Primer
(F):5'- GGGACGACATTAGATTTTTCCCAG -3'
(R):5'- GACCCATTGGAGAGGAGTTG -3'

Posted On

2014-03-28