Mutagenetix > Incidental Mutation

Incidental Mutation 'R1487:Fat4'

163507

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1487 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38941089-39066134 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 39050066 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 3976 (E3976G)

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061260
AA Change: E3976G

PolyPhen 2 Score 0.770 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743
AA Change: E3976G

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 96.4%
20x: 92.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034J05Rik	A	C	6: 146,854,877 (GRCm39)	V55G	probably benign	Het
Abhd13	A	G	8: 10,037,402 (GRCm39)		probably benign	Het
Arid5b	T	A	10: 67,933,044 (GRCm39)	K953*	probably null	Het
Armh3	A	C	19: 45,928,882 (GRCm39)		probably null	Het
B4galt6	A	G	18: 20,839,571 (GRCm39)	V121A	possibly damaging	Het
C1qtnf12	G	A	4: 156,050,331 (GRCm39)	E223K	probably damaging	Het
Calu	A	G	6: 29,366,955 (GRCm39)	I208V	probably benign	Het
Cd14	T	C	18: 36,858,537 (GRCm39)	N306S	probably benign	Het
Cdc16	T	A	8: 13,821,445 (GRCm39)	N415K	probably benign	Het
Cfap57	C	A	4: 118,471,978 (GRCm39)	V134F	probably benign	Het
Chrna2	C	T	14: 66,380,812 (GRCm39)	A27V	probably benign	Het
Chtf18	T	C	17: 25,939,583 (GRCm39)	K67R	probably benign	Het
Clock	T	C	5: 76,414,201 (GRCm39)		probably null	Het
Cplane1	C	T	15: 8,215,715 (GRCm39)	R424W	probably damaging	Het
Eif4g1	G	A	16: 20,497,623 (GRCm39)		probably benign	Het
Eps8l2	A	T	7: 140,941,531 (GRCm39)	M601L	probably benign	Het
Flrt3	T	A	2: 140,502,854 (GRCm39)	H258L	probably damaging	Het
Flt4	C	A	11: 49,523,971 (GRCm39)	T517K	possibly damaging	Het
Galnt7	T	C	8: 57,993,073 (GRCm39)	N416S	probably damaging	Het
Gipc1	C	T	8: 84,387,808 (GRCm39)	Q63*	probably null	Het
Gm17079	A	T	14: 51,930,542 (GRCm39)		probably null	Het
Gucy2c	T	A	6: 136,725,824 (GRCm39)	I375F	possibly damaging	Het
Hps4	C	T	5: 112,525,865 (GRCm39)	Q629*	probably null	Het
Hunk	A	G	16: 90,183,525 (GRCm39)	Y61C	probably damaging	Het
Itga6	T	C	2: 71,673,584 (GRCm39)	S873P	possibly damaging	Het
Kcnc1	A	G	7: 46,047,298 (GRCm39)	H66R	possibly damaging	Het
Kcnc1	T	C	7: 46,084,772 (GRCm39)		probably null	Het
Khdc3	A	G	9: 73,009,846 (GRCm39)	T19A	probably benign	Het
Kmt2a	T	C	9: 44,745,287 (GRCm39)		probably benign	Het
Lipe	C	T	7: 25,084,240 (GRCm39)	A615T	possibly damaging	Het
Lrguk	A	T	6: 34,039,295 (GRCm39)	M269L	probably benign	Het
Lrrc18	A	G	14: 32,730,640 (GRCm39)	N60D	probably damaging	Het
Magi1	T	C	6: 93,685,060 (GRCm39)	T773A	probably benign	Het
Map3k14	T	A	11: 103,116,163 (GRCm39)	D755V	possibly damaging	Het
Mecom	T	C	3: 30,034,213 (GRCm39)	T488A	probably damaging	Het
Mmp10	T	G	9: 7,509,978 (GRCm39)	W473G	probably damaging	Het
Mrgpra2a	C	T	7: 47,076,434 (GRCm39)	V275I	probably benign	Het
Myo7a	A	C	7: 97,703,017 (GRCm39)		probably null	Het
Nadsyn1	C	T	7: 143,360,662 (GRCm39)	V369I	probably benign	Het
Nptxr	C	A	15: 79,674,104 (GRCm39)	G424V	probably damaging	Het
Nrg2	C	T	18: 36,185,965 (GRCm39)	G258E	possibly damaging	Het
Nup210	G	A	6: 91,019,558 (GRCm39)	P221S	probably damaging	Het
Odad2	A	T	18: 7,273,245 (GRCm39)	Y282N	probably damaging	Het
Or52a33	T	A	7: 103,288,801 (GRCm39)	H182L	probably damaging	Het
Or6c208	T	C	10: 129,224,209 (GRCm39)	F236L	probably benign	Het
Oxct1	A	G	15: 4,177,057 (GRCm39)	D477G	possibly damaging	Het
Pcdh8	G	T	14: 80,006,987 (GRCm39)	D525E	probably damaging	Het
Phldb2	A	G	16: 45,609,387 (GRCm39)	S740P	probably damaging	Het
Prss54	A	G	8: 96,286,276 (GRCm39)	S266P	probably benign	Het
Ptprh	T	A	7: 4,555,737 (GRCm39)	I741F	probably damaging	Het
Rab11fip1	G	T	8: 27,644,240 (GRCm39)	S515Y	probably damaging	Het
Recql4	T	A	15: 76,593,183 (GRCm39)	N309I	probably benign	Het
Sec24a	T	C	11: 51,622,713 (GRCm39)	T388A	possibly damaging	Het
Setd6	T	A	8: 96,444,556 (GRCm39)	L83H	probably damaging	Het
Slc6a12	G	A	6: 121,340,716 (GRCm39)	W534*	probably null	Het
St14	C	T	9: 31,008,476 (GRCm39)	C488Y	probably damaging	Het
Supt16	A	G	14: 52,414,065 (GRCm39)		probably null	Het
Tanc2	A	G	11: 105,814,460 (GRCm39)	Y1968C	probably damaging	Het
Tcaim	T	A	9: 122,647,897 (GRCm39)	Y137*	probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Tpm3	A	T	3: 89,997,389 (GRCm39)		probably null	Het
Trip12	A	T	1: 84,746,352 (GRCm39)	N475K	probably damaging	Het
Twnk	G	T	19: 44,996,815 (GRCm39)		probably null	Het
Zfp287	T	C	11: 62,616,115 (GRCm39)	K192R	probably damaging	Het
Zfp799	G	T	17: 33,039,651 (GRCm39)	T204N	possibly damaging	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	39,036,398 (GRCm39)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,943,188 (GRCm39)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	39,035,294 (GRCm39)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,944,822 (GRCm39)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,944,728 (GRCm39)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	39,011,418 (GRCm39)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,061,350 (GRCm39)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	39,034,807 (GRCm39)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,944,140 (GRCm39)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,941,647 (GRCm39)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,945,330 (GRCm39)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,942,219 (GRCm39)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	39,003,683 (GRCm39)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,063,406 (GRCm39)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,941,907 (GRCm39)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,943,096 (GRCm39)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,064,524 (GRCm39)	nonsense	probably null
IGL01626:Fat4	APN	3	39,005,181 (GRCm39)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	39,045,880 (GRCm39)	nonsense	probably null
IGL01800:Fat4	APN	3	39,035,878 (GRCm39)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,942,922 (GRCm39)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	39,024,768 (GRCm39)	splice site	probably benign
IGL01917:Fat4	APN	3	38,943,879 (GRCm39)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	39,033,923 (GRCm39)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,064,420 (GRCm39)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,943,348 (GRCm39)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	39,037,088 (GRCm39)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,942,553 (GRCm39)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	39,050,354 (GRCm39)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,944,695 (GRCm39)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	39,005,412 (GRCm39)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,946,002 (GRCm39)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,055,288 (GRCm39)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	39,034,068 (GRCm39)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,064,137 (GRCm39)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,941,779 (GRCm39)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	39,005,280 (GRCm39)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	39,037,195 (GRCm39)	missense	probably benign
IGL02478:Fat4	APN	3	38,942,364 (GRCm39)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,064,579 (GRCm39)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,941,394 (GRCm39)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,056,913 (GRCm39)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,056,985 (GRCm39)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	39,037,486 (GRCm39)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	39,005,235 (GRCm39)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,944,744 (GRCm39)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,943,444 (GRCm39)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	39,010,893 (GRCm39)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	39,005,422 (GRCm39)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,944,992 (GRCm39)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	39,011,304 (GRCm39)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	39,012,674 (GRCm39)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	39,036,740 (GRCm39)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	39,035,701 (GRCm39)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	39,011,008 (GRCm39)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	39,045,834 (GRCm39)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	39,011,547 (GRCm39)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	39,035,390 (GRCm39)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,063,852 (GRCm39)	missense	probably benign
IGL03371:Fat4	APN	3	39,037,336 (GRCm39)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,943,283 (GRCm39)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	39,011,376 (GRCm39)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,946,168 (GRCm39)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,063,513 (GRCm39)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	39,012,599 (GRCm39)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,945,325 (GRCm39)	missense	probably damaging	1.00
Asahi	UTSW	3	39,035,968 (GRCm39)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,943,798 (GRCm39)	missense	probably benign	0.00
heineken	UTSW	3	39,034,529 (GRCm39)	missense	probably damaging	1.00
schlitz	UTSW	3	39,034,808 (GRCm39)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	39,036,506 (GRCm39)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,943,153 (GRCm39)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	39,036,652 (GRCm39)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	39,036,652 (GRCm39)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,943,080 (GRCm39)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	39,034,397 (GRCm39)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	39,034,397 (GRCm39)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,945,745 (GRCm39)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,944,965 (GRCm39)	missense	probably benign
R0357:Fat4	UTSW	3	38,945,376 (GRCm39)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	39,031,562 (GRCm39)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	39,034,786 (GRCm39)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,057,073 (GRCm39)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,942,463 (GRCm39)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	39,035,870 (GRCm39)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,997,019 (GRCm39)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,054,321 (GRCm39)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,943,843 (GRCm39)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,055,327 (GRCm39)	missense	probably benign
R0729:Fat4	UTSW	3	39,054,444 (GRCm39)	splice site	probably benign
R0748:Fat4	UTSW	3	38,941,977 (GRCm39)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	39,011,623 (GRCm39)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	39,011,623 (GRCm39)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	39,053,258 (GRCm39)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	39,050,147 (GRCm39)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	39,037,007 (GRCm39)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,945,541 (GRCm39)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	39,011,376 (GRCm39)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	39,037,235 (GRCm39)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	39,033,974 (GRCm39)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	39,037,091 (GRCm39)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,944,520 (GRCm39)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,944,962 (GRCm39)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,942,472 (GRCm39)	missense	probably damaging	1.00
R1511:Fat4	UTSW	3	39,037,225 (GRCm39)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,944,238 (GRCm39)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,943,135 (GRCm39)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,943,009 (GRCm39)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,061,326 (GRCm39)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	39,011,467 (GRCm39)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	39,034,928 (GRCm39)	missense	probably damaging	1.00
R1710:Fat4	UTSW	3	39,005,304 (GRCm39)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,945,459 (GRCm39)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,941,638 (GRCm39)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,064,417 (GRCm39)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	39,037,607 (GRCm39)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	39,037,720 (GRCm39)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	39,036,532 (GRCm39)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,064,633 (GRCm39)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	39,035,221 (GRCm39)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	39,045,813 (GRCm39)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	39,050,239 (GRCm39)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,941,264 (GRCm39)	missense	probably benign
R2056:Fat4	UTSW	3	38,945,319 (GRCm39)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,945,319 (GRCm39)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,945,319 (GRCm39)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,064,804 (GRCm39)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,943,822 (GRCm39)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	39,035,633 (GRCm39)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	39,034,882 (GRCm39)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	39,037,544 (GRCm39)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	39,037,544 (GRCm39)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,941,688 (GRCm39)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	39,035,566 (GRCm39)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,945,676 (GRCm39)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,064,364 (GRCm39)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,946,198 (GRCm39)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,943,138 (GRCm39)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,944,571 (GRCm39)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	39,050,048 (GRCm39)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	39,034,160 (GRCm39)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	39,011,254 (GRCm39)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	39,034,568 (GRCm39)	missense	probably benign
R2412:Fat4	UTSW	3	39,011,221 (GRCm39)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	39,034,953 (GRCm39)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	39,036,485 (GRCm39)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,061,302 (GRCm39)	missense	probably benign
R3103:Fat4	UTSW	3	38,946,089 (GRCm39)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,944,940 (GRCm39)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	39,035,423 (GRCm39)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	39,036,587 (GRCm39)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,061,410 (GRCm39)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,061,410 (GRCm39)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	39,036,495 (GRCm39)	missense	probably benign
R4065:Fat4	UTSW	3	39,063,346 (GRCm39)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	39,034,169 (GRCm39)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,942,024 (GRCm39)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,996,958 (GRCm39)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,945,776 (GRCm39)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,943,320 (GRCm39)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,945,912 (GRCm39)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	39,012,689 (GRCm39)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,945,443 (GRCm39)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	39,005,432 (GRCm39)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,941,491 (GRCm39)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	39,037,106 (GRCm39)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,942,466 (GRCm39)	missense	probably benign
R4871:Fat4	UTSW	3	38,945,754 (GRCm39)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	39,034,781 (GRCm39)	missense	probably damaging	1.00
R4928:Fat4	UTSW	3	39,064,614 (GRCm39)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,061,352 (GRCm39)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	39,011,601 (GRCm39)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	39,034,322 (GRCm39)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	39,037,195 (GRCm39)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	39,037,195 (GRCm39)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,942,438 (GRCm39)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	39,034,946 (GRCm39)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	39,034,340 (GRCm39)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	39,011,017 (GRCm39)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,064,527 (GRCm39)	missense	probably benign	0.44
R5337:Fat4	UTSW	3	38,945,776 (GRCm39)	missense	probably damaging	1.00
R5363:Fat4	UTSW	3	38,942,154 (GRCm39)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,943,013 (GRCm39)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	39,050,095 (GRCm39)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,941,394 (GRCm39)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,945,495 (GRCm39)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,064,519 (GRCm39)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,941,364 (GRCm39)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,064,423 (GRCm39)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,943,083 (GRCm39)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,057,117 (GRCm39)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,943,774 (GRCm39)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	39,003,725 (GRCm39)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	39,037,283 (GRCm39)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,943,617 (GRCm39)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	39,035,104 (GRCm39)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,945,936 (GRCm39)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,061,454 (GRCm39)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,941,485 (GRCm39)	missense	probably benign
R5933:Fat4	UTSW	3	39,005,524 (GRCm39)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	39,005,388 (GRCm39)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,943,798 (GRCm39)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	39,037,355 (GRCm39)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,064,696 (GRCm39)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,056,951 (GRCm39)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	39,037,411 (GRCm39)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,945,870 (GRCm39)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	39,005,505 (GRCm39)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,061,395 (GRCm39)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,061,229 (GRCm39)	splice site	probably null
R6387:Fat4	UTSW	3	39,037,934 (GRCm39)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	39,034,529 (GRCm39)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	39,038,128 (GRCm39)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,945,036 (GRCm39)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	39,035,418 (GRCm39)	nonsense	probably null
R6503:Fat4	UTSW	3	39,036,406 (GRCm39)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,057,020 (GRCm39)	missense	probably benign
R6566:Fat4	UTSW	3	39,011,275 (GRCm39)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	39,033,839 (GRCm39)	missense	probably benign
R6590:Fat4	UTSW	3	39,037,688 (GRCm39)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,997,077 (GRCm39)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	39,010,970 (GRCm39)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	39,037,688 (GRCm39)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	39,036,589 (GRCm39)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	39,038,088 (GRCm39)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	39,011,674 (GRCm39)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	39,035,966 (GRCm39)	missense	probably benign	0.00
R6925:Fat4	UTSW	3	39,050,353 (GRCm39)	critical splice donor site	probably null
R6948:Fat4	UTSW	3	39,063,595 (GRCm39)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	39,050,120 (GRCm39)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	39,035,924 (GRCm39)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,945,692 (GRCm39)	missense	probably benign
R7030:Fat4	UTSW	3	39,036,107 (GRCm39)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,064,960 (GRCm39)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,064,959 (GRCm39)	missense	probably benign
R7045:Fat4	UTSW	3	38,942,750 (GRCm39)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,944,023 (GRCm39)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,064,682 (GRCm39)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	39,034,936 (GRCm39)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	39,034,808 (GRCm39)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	39,034,613 (GRCm39)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	39,038,044 (GRCm39)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,943,033 (GRCm39)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	39,031,511 (GRCm39)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	39,053,236 (GRCm39)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,945,192 (GRCm39)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	39,034,325 (GRCm39)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,944,562 (GRCm39)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	39,037,989 (GRCm39)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,943,842 (GRCm39)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,943,294 (GRCm39)	nonsense	probably null
R7372:Fat4	UTSW	3	38,944,358 (GRCm39)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,942,073 (GRCm39)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,054,385 (GRCm39)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,063,793 (GRCm39)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,941,599 (GRCm39)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,063,306 (GRCm39)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	39,011,576 (GRCm39)	nonsense	probably null
R7501:Fat4	UTSW	3	39,012,597 (GRCm39)	nonsense	probably null
R7533:Fat4	UTSW	3	39,061,406 (GRCm39)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	39,035,770 (GRCm39)	missense	possibly damaging	0.64
R7542:Fat4	UTSW	3	39,035,504 (GRCm39)	missense	possibly damaging	0.56
R7548:Fat4	UTSW	3	39,035,263 (GRCm39)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,943,485 (GRCm39)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,064,390 (GRCm39)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	39,035,309 (GRCm39)	missense	probably benign
R7665:Fat4	UTSW	3	38,943,327 (GRCm39)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,945,846 (GRCm39)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,055,353 (GRCm39)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,942,000 (GRCm39)	missense	probably benign
R7883:Fat4	UTSW	3	39,035,968 (GRCm39)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	39,003,588 (GRCm39)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,941,690 (GRCm39)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,945,870 (GRCm39)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	39,036,065 (GRCm39)	missense	possibly damaging	0.79
R8040:Fat4	UTSW	3	39,035,815 (GRCm39)	missense	probably damaging	1.00
R8142:Fat4	UTSW	3	38,945,352 (GRCm39)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,946,203 (GRCm39)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	39,033,881 (GRCm39)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	39,012,659 (GRCm39)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,063,128 (GRCm39)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	39,033,824 (GRCm39)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	39,035,702 (GRCm39)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	39,036,052 (GRCm39)	missense	probably benign
R8543:Fat4	UTSW	3	39,031,643 (GRCm39)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,064,842 (GRCm39)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,064,647 (GRCm39)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,942,592 (GRCm39)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,946,002 (GRCm39)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	39,033,898 (GRCm39)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	39,053,278 (GRCm39)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,946,269 (GRCm39)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	39,037,778 (GRCm39)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,941,324 (GRCm39)	nonsense	probably null
R9071:Fat4	UTSW	3	39,037,598 (GRCm39)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,061,448 (GRCm39)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,064,803 (GRCm39)	missense
R9180:Fat4	UTSW	3	38,942,556 (GRCm39)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	39,036,592 (GRCm39)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,945,079 (GRCm39)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,063,390 (GRCm39)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,942,396 (GRCm39)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,945,171 (GRCm39)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,945,781 (GRCm39)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	39,036,047 (GRCm39)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,945,417 (GRCm39)	missense	probably benign	0.00
R9455:Fat4	UTSW	3	38,945,412 (GRCm39)	missense
R9456:Fat4	UTSW	3	38,942,571 (GRCm39)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	39,037,886 (GRCm39)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	39,037,886 (GRCm39)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	39,034,802 (GRCm39)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,063,346 (GRCm39)	missense	probably benign
R9568:Fat4	UTSW	3	38,946,156 (GRCm39)	missense	probably damaging	1.00
R9646:Fat4	UTSW	3	39,035,813 (GRCm39)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,943,332 (GRCm39)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,055,374 (GRCm39)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,063,255 (GRCm39)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	39,035,189 (GRCm39)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,054,300 (GRCm39)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,997,051 (GRCm39)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	39,024,901 (GRCm39)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	39,012,641 (GRCm39)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,941,199 (GRCm39)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	39,037,964 (GRCm39)	missense	probably damaging	1.00
Z1176:Fat4	UTSW	3	39,037,508 (GRCm39)	missense	probably benign	0.00
Z1177:Fat4	UTSW	3	38,944,496 (GRCm39)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,942,733 (GRCm39)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	39,035,987 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGACCAGCTTGCCAATGATGAC -3'
(R):5'- GTCCCACTGCCCAGATTGTATGAG -3'

Sequencing Primer
(F):5'- GAGTTTCATCTGGTTACAAGAATGGC -3'
(R):5'- TGCCCAGATTGTATGAGAATCTGAG -3'

Posted On

2014-03-28