Mutagenetix > Incidental Mutations

Incidental Mutation 'R1479:Dclk3'

164206

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Click-I, -II related, Dcamkl3

MMRRC Submission

039532-MU

Accession Numbers

Genbank: NM_172928; MGI: 3039580

Is this an essential gene?

Possibly non essential (E-score: 0.366) question?

Stock #

R1479 (G1)

Quality Score

189

Status

Validated

Chromosome

Chromosomal Location

111439081-111489118 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 111468546 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Asparagine at position 386 (S386N)

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

Predicted Effect

probably benign
Transcript: ENSMUST00000111879
AA Change: S386N

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: S386N

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Meta Mutation Damage Score

0.1136

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.3%

Validation Efficiency

96% (81/84)

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	T	A	12: 55,379,387	D138E	probably damaging	Het
1700022I11Rik	A	C	4: 42,972,543	K625N	possibly damaging	Het
2310030G06Rik	T	A	9: 50,741,301	T58S	possibly damaging	Het
4930432K21Rik	C	A	8: 84,162,397	T123K	possibly damaging	Het
Alox12e	A	G	11: 70,320,782	V252A	probably benign	Het
Anks6	T	C	4: 47,044,874	D344G	probably damaging	Het
Atg14	A	T	14: 47,547,239		probably null	Het
BC052040	T	A	2: 115,639,013	N74K	probably benign	Het
Bcr	G	T	10: 75,061,125	E34*	probably null	Het
Birc6	C	T	17: 74,634,853	T2728M	probably damaging	Het
Bmp2k	T	A	5: 97,053,200	N326K	probably benign	Het
Ccdc188	A	C	16: 18,219,290	T242P	possibly damaging	Het
Chsy3	A	T	18: 59,408,913	E374D	probably benign	Het
Clca4b	A	T	3: 144,915,468	V615E	probably damaging	Het
Clcnka	C	A	4: 141,389,447	A498S	possibly damaging	Het
Csmd3	A	G	15: 47,857,886	C1450R	probably damaging	Het
Cul7	C	A	17: 46,651,747	D101E	probably damaging	Het
Cyp27b1	G	A	10: 127,051,711		probably null	Het
Cyp2d22	A	G	15: 82,371,936	S404P	probably damaging	Het
Dnah10	G	A	5: 124,777,889	D1953N	possibly damaging	Het
Dst	T	A	1: 34,264,515		probably null	Het
Egfem1	A	G	3: 29,657,165	N241D	probably damaging	Het
Entpd7	T	C	19: 43,721,840	F312S	probably damaging	Het
Esp34	T	A	17: 38,554,328		probably benign	Het
Fam126b	T	A	1: 58,552,268	R91*	probably null	Het
Foxd2	T	A	4: 114,907,918	T302S	unknown	Het
Fzd6	A	T	15: 39,030,999	N187Y	probably damaging	Het
Gbp9	C	T	5: 105,094,064		probably benign	Het
Gm11492	G	T	11: 87,567,418	R206L	probably damaging	Het
Gna14	T	C	19: 16,533,769	S61P	possibly damaging	Het
Grap	A	T	11: 61,660,298	Y52F	probably benign	Het
H2-T3	T	C	17: 36,189,428	Y125C	probably damaging	Het
Hax1	C	A	3: 89,995,857	E212D	probably damaging	Het
Hecw1	A	C	13: 14,316,492	S638R	probably benign	Het
Hira	A	T	16: 18,896,469	K39M	probably damaging	Het
Hoxa2	T	A	6: 52,163,340	D222V	probably damaging	Het
Jph2	C	T	2: 163,339,271	V658M	possibly damaging	Het
Kansl1	A	T	11: 104,342,416	S762T	probably damaging	Het
Kat6b	T	A	14: 21,618,956	C267S	probably benign	Het
Klk6	A	G	7: 43,831,634	N250S	probably benign	Het
Lbp	T	C	2: 158,319,714	L232S	probably damaging	Het
Lcn9	A	T	2: 25,823,703		probably benign	Het
Lcp2	A	G	11: 34,075,068	H213R	probably benign	Het
Lrrc9	A	T	12: 72,460,825	K367*	probably null	Het
Lyst	A	G	13: 13,634,482	I246V	probably benign	Het
Megf6	G	T	4: 154,177,121	V68L	probably benign	Het
Mst1r	T	C	9: 107,913,345		probably benign	Het
Myo18a	A	G	11: 77,842,194	E909G	probably benign	Het
Nipbl	A	T	15: 8,350,289	D1006E	probably benign	Het
Olfr1164	A	G	2: 88,093,286	F217L	probably benign	Het
Olfr729	C	A	14: 50,148,788	V29F	probably benign	Het
Olfr933	A	T	9: 38,975,762	I29F	probably benign	Het
Otog	A	G	7: 46,295,978	I2220V	possibly damaging	Het
Pcx	T	A	19: 4,602,024	I99N	probably damaging	Het
Pi4ka	C	T	16: 17,373,400	G211D	probably benign	Het
Pp2d1	T	C	17: 53,507,855	S614G	probably benign	Het
Prdx6	G	A	1: 161,244,263	A111V	probably damaging	Het
Prss51	G	A	14: 64,096,170		probably null	Het
Psmd6	C	T	14: 14,116,819		probably benign	Het
Pten	T	A	19: 32,819,850	L345Q	probably damaging	Het
Qrich2	T	G	11: 116,441,485	H2295P	probably benign	Het
Rgs11	T	C	17: 26,208,283		probably null	Het
Rgs6	A	G	12: 83,116,244	E408G	probably damaging	Het
Slc38a7	T	C	8: 95,848,494	T53A	probably benign	Het
Sptbn1	A	G	11: 30,113,909	C1957R	probably damaging	Het
Sumf1	T	C	6: 108,176,058	Y123C	probably damaging	Het
Tnrc6b	T	A	15: 80,887,032		probably null	Het
Ttc21a	C	A	9: 119,956,947	D670E	probably benign	Het
Ttn	A	G	2: 76,744,511	V25346A	probably damaging	Het
Ubr4	A	G	4: 139,425,840	T2070A	possibly damaging	Het
Vmn2r57	C	A	7: 41,427,830	W304L	possibly damaging	Het
Vps13a	T	C	19: 16,750,114		probably benign	Het
Wisp3	G	A	10: 39,153,243	R230W	probably damaging	Het
Zfp647	A	G	15: 76,911,203	V419A	possibly damaging	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111467843	nonsense	probably null
IGL02125:Dclk3	APN	9	111469107	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111469023	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111488673	utr 3 prime	probably benign
IGL02984:Dclk3	UTSW	9	111488575	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111484935	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111469163	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111482721	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111469070	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111469020	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111469236	missense	probably benign	0.43
R1482:Dclk3	UTSW	9	111467820	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111468054	missense	probably benign	0.04
R1552:Dclk3	UTSW	9	111488579	missense	probably damaging	1.00
R1559:Dclk3	UTSW	9	111469208	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111468354	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111488542	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111469080	missense	probably damaging	0.99
R4510:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4511:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111467895	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111469185	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111468648	missense	probably benign
R4932:Dclk3	UTSW	9	111468042	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111467788	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111468681	missense	probably benign
R5338:Dclk3	UTSW	9	111469059	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111469260	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
X0020:Dclk3	UTSW	9	111485075	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTCAGGAGGAGACTCATGCAAGTG -3'
(R):5'- TCATCTTCATGGCGTAAGCCTGC -3'

Sequencing Primer
(F):5'- CTCATGCAAGTGGAGAGAAACATC -3'
(R):5'- ATTCCTTCACGGTGGCAAAG -3'

Posted On

2014-03-28