Mutagenetix > Incidental Mutation

Incidental Mutation 'R1531:Or14j10'

166254

Institutional Source

Beutler Lab

Gene Symbol

Or14j10

Ensembl Gene

ENSMUSG00000061336

Gene Name

olfactory receptor family 14 subfamily J member 10

Synonyms

Olfr116, MOR218-2, GA_x6K02T2PSCP-2084102-2083137

MMRRC Submission

039570-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R1531 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

37934559-37935524 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 37935243 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 94 (Y94*)

Ref Sequence

ENSEMBL: ENSMUSP00000150977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072265] [ENSMUST00000216128] [ENSMUST00000223366]

AlphaFold

Q923Q6

Predicted Effect

probably null
Transcript: ENSMUST00000072265
AA Change: Y94*

SMART Domains

Protein: ENSMUSP00000072115
Gene: ENSMUSG00000061336
AA Change: Y94*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.9e-45	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.1e-6	PFAM
Pfam:7tm_1	41	290	6.1e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216128
AA Change: Y94*

Predicted Effect

probably benign
Transcript: ENSMUST00000223366

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agtpbp1	T	A	13: 59,648,448 (GRCm39)		probably null	Het
Aldh3b2	T	G	19: 4,027,543 (GRCm39)	F28C	probably damaging	Het
Apaf1	T	C	10: 90,890,383 (GRCm39)	N540S	probably damaging	Het
Apob	T	C	12: 8,047,880 (GRCm39)	I953T	possibly damaging	Het
Arhgef1	A	G	7: 24,624,423 (GRCm39)	T816A	probably damaging	Het
Arid4a	T	A	12: 71,122,779 (GRCm39)	F1053L	probably benign	Het
Atxn1l	A	C	8: 110,458,691 (GRCm39)	F524V	probably damaging	Het
Cad	T	A	5: 31,233,563 (GRCm39)	M1874K	probably benign	Het
Ccdc40	G	A	11: 119,154,015 (GRCm39)	V1096I	probably benign	Het
Ccdc93	A	G	1: 121,408,551 (GRCm39)	D358G	probably benign	Het
Cd96	T	C	16: 45,938,169 (GRCm39)	T99A	probably benign	Het
Cdc42ep3	A	T	17: 79,642,473 (GRCm39)	M149K	probably benign	Het
Cgas	A	G	9: 78,349,763 (GRCm39)	Y200H	probably damaging	Het
Cog4	A	G	8: 111,606,353 (GRCm39)	E613G	probably benign	Het
Crebbp	A	T	16: 3,902,381 (GRCm39)	I2286N	probably benign	Het
Csf1	T	C	3: 107,655,654 (GRCm39)	E459G	possibly damaging	Het
Csrp2	A	T	10: 110,771,066 (GRCm39)	Y57F	probably benign	Het
Ctdspl	C	T	9: 118,869,650 (GRCm39)	P244L	probably damaging	Het
Cyp2c29	A	G	19: 39,313,412 (GRCm39)	R303G	probably damaging	Het
Cyp4a10	C	A	4: 115,375,632 (GRCm39)	Y38*	probably null	Het
Dmgdh	T	A	13: 93,880,919 (GRCm39)	I783N	probably damaging	Het
Efcc1	G	A	6: 87,708,148 (GRCm39)	E92K	probably benign	Het
Eif2ak4	T	A	2: 118,273,691 (GRCm39)	L872H	probably damaging	Het
Elp2	T	A	18: 24,764,461 (GRCm39)	S603T	probably benign	Het
Eml2	T	A	7: 18,930,179 (GRCm39)	L300H	probably damaging	Het
Esp3	A	G	17: 40,946,827 (GRCm39)	K50R	possibly damaging	Het
Esrp1	A	G	4: 11,379,375 (GRCm39)	F136L	probably damaging	Het
Exoc1	T	A	5: 76,707,011 (GRCm39)	D497E	probably damaging	Het
Fat3	A	G	9: 15,908,761 (GRCm39)	S2414P	probably damaging	Het
Foxj3	C	T	4: 119,477,398 (GRCm39)	P369S	unknown	Het
Gkn2	G	T	6: 87,352,921 (GRCm39)		probably null	Het
Gm17333	AAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	AAGAAGAGAAGAGAAGAGAAGAGAAGAGAA	16: 77,649,766 (GRCm39)		noncoding transcript	Het
Gm21905	A	T	5: 68,103,724 (GRCm39)		probably benign	Het
Grhl1	T	A	12: 24,632,962 (GRCm39)		probably null	Het
Grk6	A	G	13: 55,599,967 (GRCm39)	Y221C	probably damaging	Het
Hcrtr1	T	A	4: 130,024,720 (GRCm39)	K389*	probably null	Het
Hk1	G	A	10: 62,120,563 (GRCm39)	R545C	probably damaging	Het
Hsp90b1	A	T	10: 86,532,659 (GRCm39)	I339N	probably benign	Het
Ikzf3	T	C	11: 98,381,272 (GRCm39)	R103G	probably damaging	Het
Itgad	A	C	7: 127,777,542 (GRCm39)	I141L	probably benign	Het
Jmjd6	T	A	11: 116,733,266 (GRCm39)	Y137F	probably benign	Het
Kcna1	A	G	6: 126,619,030 (GRCm39)	V430A	probably benign	Het
Kel	A	G	6: 41,665,560 (GRCm39)	V520A	probably damaging	Het
Klhl41	G	A	2: 69,501,084 (GRCm39)	V182I	probably benign	Het
Ldhb	C	A	6: 142,447,121 (GRCm39)	M64I	probably benign	Het
Lrrc23	A	G	6: 124,753,077 (GRCm39)	S190P	possibly damaging	Het
Mboat2	T	C	12: 25,009,029 (GRCm39)	V445A	probably benign	Het
Mlycd	G	A	8: 120,128,258 (GRCm39)	W188*	probably null	Het
Mpp3	C	T	11: 101,899,475 (GRCm39)	E349K	probably benign	Het
Mtres1	A	G	10: 43,401,316 (GRCm39)	V211A	probably benign	Het
Myh4	A	G	11: 67,141,366 (GRCm39)	M780V	probably benign	Het
Myh6	G	A	14: 55,193,963 (GRCm39)	R809C	probably damaging	Het
Mylip	G	A	13: 45,560,046 (GRCm39)	V161M	possibly damaging	Het
Nrp1	G	A	8: 129,152,450 (GRCm39)	V220I	probably null	Het
Nup210	A	T	6: 91,011,823 (GRCm39)	N455K	probably benign	Het
Or51f1d	C	A	7: 102,700,795 (GRCm39)	Q97K	probably benign	Het
Pbld2	G	T	10: 62,889,732 (GRCm39)		probably null	Het
Pclo	C	G	5: 14,571,917 (GRCm39)	P434R	probably damaging	Het
Pdlim3	A	G	8: 46,349,800 (GRCm39)	K37E	probably damaging	Het
Prkdc	T	A	16: 15,589,970 (GRCm39)	I2611N	probably benign	Het
Prr12	T	C	7: 44,677,954 (GRCm39)	D2019G	unknown	Het
Rab27a	A	G	9: 73,002,685 (GRCm39)	T205A	probably benign	Het
Rcan3	A	G	4: 135,152,595 (GRCm39)	L42P	probably damaging	Het
Rchy1	T	C	5: 92,103,474 (GRCm39)		probably null	Het
Sema6a	T	C	18: 47,382,066 (GRCm39)	H827R	probably damaging	Het
Sertad4	A	G	1: 192,533,258 (GRCm39)		probably null	Het
Smarcd1	T	A	15: 99,605,264 (GRCm39)	C282S	probably damaging	Het
Speg	A	G	1: 75,377,866 (GRCm39)	T769A	possibly damaging	Het
Syne1	T	A	10: 5,297,875 (GRCm39)	K1141*	probably null	Het
Synpo2	T	C	3: 122,911,315 (GRCm39)	E110G	probably benign	Het
Tg	T	A	15: 66,722,351 (GRCm39)	D333E	probably benign	Het
Tmem132c	A	G	5: 127,436,955 (GRCm39)	Y148C	probably damaging	Het
Tmem215	T	A	4: 40,473,965 (GRCm39)	V14E	probably damaging	Het
Tmem229b	A	G	12: 79,011,685 (GRCm39)	L82P	probably damaging	Het
Togaram1	A	G	12: 65,013,039 (GRCm39)	T97A	probably benign	Het
Trappc9	G	A	15: 72,565,397 (GRCm39)	R965*	probably null	Het
Trio	T	C	15: 27,833,071 (GRCm39)	I104V	probably benign	Het
Trp73	A	G	4: 154,148,352 (GRCm39)	F354S	probably benign	Het
Ttyh2	T	C	11: 114,577,278 (GRCm39)	L63P	probably damaging	Het
Ulk4	G	C	9: 120,873,841 (GRCm39)	P1197A	probably damaging	Het
Vmn1r176	T	C	7: 23,534,536 (GRCm39)	M206V	possibly damaging	Het
Vps8	T	A	16: 21,285,226 (GRCm39)	Y402*	probably null	Het
Zbtb41	A	G	1: 139,350,931 (GRCm39)	I15V	probably benign	Het
Zc3hav1	T	C	6: 38,284,170 (GRCm39)	I982V	possibly damaging	Het
Zmynd19	T	A	2: 24,848,123 (GRCm39)	N106K	probably benign	Het

Other mutations in Or14j10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02434:Or14j10	APN	17	37,935,467 (GRCm39)	missense	possibly damaging	0.86
IGL02639:Or14j10	APN	17	37,934,878 (GRCm39)	missense	probably benign	0.01
IGL02663:Or14j10	APN	17	37,934,935 (GRCm39)	missense	probably benign	0.37
R0049:Or14j10	UTSW	17	37,935,024 (GRCm39)	missense	probably benign	0.02
R1260:Or14j10	UTSW	17	37,934,594 (GRCm39)	missense	probably benign	0.00
R3236:Or14j10	UTSW	17	37,935,127 (GRCm39)	missense	possibly damaging	0.65
R4083:Or14j10	UTSW	17	37,935,316 (GRCm39)	missense	probably damaging	1.00
R4308:Or14j10	UTSW	17	37,934,627 (GRCm39)	missense	possibly damaging	0.46
R4887:Or14j10	UTSW	17	37,934,782 (GRCm39)	missense	probably damaging	0.99
R5167:Or14j10	UTSW	17	37,934,642 (GRCm39)	nonsense	probably null
R5323:Or14j10	UTSW	17	37,935,046 (GRCm39)	missense	probably benign	0.22
R5496:Or14j10	UTSW	17	37,935,469 (GRCm39)	missense	probably benign	0.01
R5530:Or14j10	UTSW	17	37,934,698 (GRCm39)	missense	possibly damaging	0.72
R5643:Or14j10	UTSW	17	37,935,323 (GRCm39)	missense	probably benign	0.00
R5644:Or14j10	UTSW	17	37,935,323 (GRCm39)	missense	probably benign	0.00
R5798:Or14j10	UTSW	17	37,934,881 (GRCm39)	missense	probably benign	0.01
R6020:Or14j10	UTSW	17	37,934,858 (GRCm39)	missense	possibly damaging	0.94
R7058:Or14j10	UTSW	17	37,934,597 (GRCm39)	missense	probably benign	0.00
R7453:Or14j10	UTSW	17	37,935,276 (GRCm39)	missense	probably benign	0.00
R7474:Or14j10	UTSW	17	37,935,277 (GRCm39)	missense	probably benign	0.30
R7565:Or14j10	UTSW	17	37,935,392 (GRCm39)	missense	probably damaging	0.99
R7646:Or14j10	UTSW	17	37,935,295 (GRCm39)	missense	probably damaging	1.00
Z1088:Or14j10	UTSW	17	37,935,320 (GRCm39)	missense	probably damaging	0.98
Z1187:Or14j10	UTSW	17	37,934,735 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCTTAGCCATACACTTGTCACTGCC -3'
(R):5'- TTCTCTGACAACCGTGAGCTGC -3'

Sequencing Primer
(F):5'- CCACGTACACTTTCTGGGAC -3'
(R):5'- TGCTGACATACCTATTGGGC -3'

Posted On

2014-04-13