Mutagenetix > Incidental Mutation

Incidental Mutation 'R1515:Gpr35'

168198

Institutional Source

Beutler Lab

Gene Symbol

Gpr35

Ensembl Gene

ENSMUSG00000026271

Gene Name

G protein-coupled receptor 35

Synonyms

MMRRC Submission

039562-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1515 (G1)

Quality Score

166

Status

Not validated

Chromosome

Chromosomal Location

92878587-92914113 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 92910770 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Valine at position 161 (F161V)

Ref Sequence

ENSEMBL: ENSMUSP00000139648 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027489] [ENSMUST00000064480] [ENSMUST00000169198] [ENSMUST00000185421] [ENSMUST00000186298] [ENSMUST00000189697]

AlphaFold

Q9ES90

Predicted Effect

probably benign
Transcript: ENSMUST00000027489

SMART Domains

Protein: ENSMUSP00000027489
Gene: ENSMUSG00000026271

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	1.8e-34	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000064480
AA Change: F161V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000070832
Gene: ENSMUSG00000026271
AA Change: F161V

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000169198
AA Change: F161V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126914
Gene: ENSMUSG00000026271
AA Change: F161V

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000185421

Predicted Effect

probably damaging
Transcript: ENSMUST00000186298
AA Change: F161V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139648
Gene: ENSMUSG00000026271
AA Change: F161V

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000189697

SMART Domains

Protein: ENSMUSP00000139787
Gene: ENSMUSG00000026271

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	147	5.7e-6	PFAM
Pfam:7tm_1	34	147	1.4e-24	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.5%
10x: 96.8%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akt2	A	G	7: 27,336,583 (GRCm39)	T401A	probably damaging	Het
Arhgap32	G	T	9: 32,027,498 (GRCm39)	V23L	probably benign	Het
Atmin	T	A	8: 117,681,579 (GRCm39)	C193S	possibly damaging	Het
Atp13a5	C	T	16: 29,152,792 (GRCm39)	V225I	probably benign	Het
Auh	G	A	13: 52,989,532 (GRCm39)	P308L	probably benign	Het
BC024139	A	G	15: 76,008,526 (GRCm39)	V350A	possibly damaging	Het
Birc6	G	T	17: 74,835,631 (GRCm39)	E29*	probably null	Het
Bnc2	T	C	4: 84,332,563 (GRCm39)	N104S	probably null	Het
C030048H21Rik	T	C	2: 26,147,515 (GRCm39)		probably null	Het
Cd320	G	T	17: 34,066,613 (GRCm39)	C117F	probably damaging	Het
Cdkal1	A	G	13: 29,510,133 (GRCm39)	S542P	probably damaging	Het
Crocc	T	C	4: 140,747,048 (GRCm39)	T1587A	probably benign	Het
Defb41	T	C	1: 18,330,817 (GRCm39)		probably null	Het
Dmtf1	A	G	5: 9,190,384 (GRCm39)		probably null	Het
Dnhd1	A	G	7: 105,353,355 (GRCm39)	N2836S	probably benign	Het
Dpp8	G	A	9: 64,986,030 (GRCm39)	S840N	probably benign	Het
Dsc2	A	G	18: 20,167,758 (GRCm39)	F111L	probably damaging	Het
Dsc2	T	A	18: 20,178,622 (GRCm39)	I261F	probably benign	Het
Ece1	T	C	4: 137,678,819 (GRCm39)	V509A	probably benign	Het
Ecm2	T	C	13: 49,671,808 (GRCm39)	M103T	possibly damaging	Het
Emsy	T	C	7: 98,240,063 (GRCm39)	H1064R	probably damaging	Het
Engase	T	C	11: 118,377,966 (GRCm39)	V252A	possibly damaging	Het
F13b	A	G	1: 139,438,703 (GRCm39)	Y369C	probably damaging	Het
Flii	T	C	11: 60,612,432 (GRCm39)		probably null	Het
Fzd10	T	A	5: 128,679,623 (GRCm39)	F448I	probably damaging	Het
Gprin2	T	C	14: 33,917,230 (GRCm39)	D180G	possibly damaging	Het
Grik3	A	G	4: 125,564,521 (GRCm39)	N501S	probably benign	Het
Hells	A	G	19: 38,956,209 (GRCm39)	K802E	probably damaging	Het
Il1r1	T	A	1: 40,332,509 (GRCm39)	C96*	probably null	Het
Kcnk16	T	A	14: 20,315,345 (GRCm39)	I73F	probably damaging	Het
Kcnq5	A	G	1: 21,472,905 (GRCm39)	S652P	probably benign	Het
Lamc3	A	G	2: 31,830,763 (GRCm39)	D1500G	probably damaging	Het
Macf1	A	G	4: 123,272,273 (GRCm39)	F6468L	probably damaging	Het
Mgrn1	T	A	16: 4,733,644 (GRCm39)	F198I	probably benign	Het
Mmp3	A	G	9: 7,451,232 (GRCm39)	T323A	probably benign	Het
N4bp2	A	G	5: 65,947,841 (GRCm39)	Y157C	probably benign	Het
Nfkbid	C	A	7: 30,124,781 (GRCm39)	H190Q	probably benign	Het
Or10a3m	T	C	7: 108,313,148 (GRCm39)	V184A	possibly damaging	Het
Or5b118	T	C	19: 13,449,044 (GRCm39)	S237P	probably damaging	Het
Or6c75	T	C	10: 129,337,460 (GRCm39)	S236P	probably damaging	Het
Osgin2	C	T	4: 15,998,380 (GRCm39)	G414D	probably benign	Het
Pkd1	G	A	17: 24,813,827 (GRCm39)	R4097H	probably benign	Het
Pnkd	T	A	1: 74,388,968 (GRCm39)	L213Q	probably null	Het
Ppfibp1	A	G	6: 146,928,930 (GRCm39)	H850R	probably benign	Het
Ppp6r1	T	C	7: 4,646,257 (GRCm39)	D148G	probably damaging	Het
Ptprt	A	T	2: 162,079,954 (GRCm39)	S282T	probably damaging	Het
Sgsm3	T	C	15: 80,894,457 (GRCm39)	V536A	probably benign	Het
Slc22a23	T	A	13: 34,387,947 (GRCm39)	Q383L	probably benign	Het
Snx29	T	C	16: 11,217,701 (GRCm39)		probably null	Het
Tmem229b-ps	T	A	10: 53,351,542 (GRCm39)		noncoding transcript	Het
Tmod4	A	T	3: 95,035,990 (GRCm39)	Y317F	possibly damaging	Het
Trim13	T	C	14: 61,843,108 (GRCm39)	M375T	probably benign	Het
Txndc11	A	G	16: 10,892,926 (GRCm39)	S935P	probably damaging	Het
Umod	T	C	7: 119,064,720 (GRCm39)	N592D	probably benign	Het
Vmn2r118	A	G	17: 55,917,643 (GRCm39)	Y290H	probably benign	Het
Vps26b	A	G	9: 26,924,041 (GRCm39)	M234T	probably damaging	Het
Zbtb48	A	G	4: 152,104,658 (GRCm39)		probably null	Het
Zfc3h1	T	A	10: 115,252,647 (GRCm39)	F1320Y	probably benign	Het
Zfp784	A	T	7: 5,039,039 (GRCm39)		probably benign	Het

Other mutations in Gpr35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00509:Gpr35	APN	1	92,910,594 (GRCm39)	missense	probably damaging	0.99
IGL03102:Gpr35	APN	1	92,910,299 (GRCm39)	missense	probably benign	0.00
PIT4280001:Gpr35	UTSW	1	92,910,356 (GRCm39)	missense	probably damaging	1.00
R0015:Gpr35	UTSW	1	92,910,954 (GRCm39)	missense	probably damaging	1.00
R0412:Gpr35	UTSW	1	92,910,506 (GRCm39)	missense	probably benign	0.01
R1203:Gpr35	UTSW	1	92,910,870 (GRCm39)	missense	probably damaging	0.98
R2872:Gpr35	UTSW	1	92,910,848 (GRCm39)	missense	probably benign	0.34
R2872:Gpr35	UTSW	1	92,910,848 (GRCm39)	missense	probably benign	0.34
R3498:Gpr35	UTSW	1	92,911,113 (GRCm39)	missense	probably damaging	1.00
R3499:Gpr35	UTSW	1	92,911,113 (GRCm39)	missense	probably damaging	1.00
R4732:Gpr35	UTSW	1	92,911,107 (GRCm39)	missense	probably damaging	1.00
R4733:Gpr35	UTSW	1	92,911,107 (GRCm39)	missense	probably damaging	1.00
R5270:Gpr35	UTSW	1	92,910,299 (GRCm39)	missense	probably benign	0.00
R5969:Gpr35	UTSW	1	92,910,942 (GRCm39)	missense	probably damaging	1.00
R6769:Gpr35	UTSW	1	92,910,426 (GRCm39)	missense	probably damaging	0.97
R6771:Gpr35	UTSW	1	92,910,426 (GRCm39)	missense	probably damaging	0.97
R7144:Gpr35	UTSW	1	92,910,353 (GRCm39)	missense	probably benign	0.11
R7395:Gpr35	UTSW	1	92,910,929 (GRCm39)	missense	probably damaging	1.00
R8156:Gpr35	UTSW	1	92,910,437 (GRCm39)	missense	probably damaging	1.00
Z1177:Gpr35	UTSW	1	92,910,738 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCATAAGCCTGGTCACTGCCATTG -3'
(R):5'- ATTGAGGTTCAGGGAGACCTGCAC -3'

Sequencing Primer
(F):5'- ACTGCCATTGCTGTGGAC -3'
(R):5'- TCAGGACCACATGCAAGG -3'

Posted On

2014-04-13