Mutagenetix > Incidental Mutation

Incidental Mutation 'R1537:Taar5'

169608

Institutional Source

Beutler Lab

Gene Symbol

Taar5

Ensembl Gene

ENSMUSG00000069706

Gene Name

trace amine-associated receptor 5

Synonyms

LOC215854

MMRRC Submission

039576-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R1537 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23846604-23847617 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23846620 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Histidine at position 6 (L6H)

Ref Sequence

ENSEMBL: ENSMUSP00000090329 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092659]

AlphaFold

Q5QD14

Predicted Effect

probably benign
Transcript: ENSMUST00000092659
AA Change: L6H

PolyPhen 2 Score 0.255 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000090329
Gene: ENSMUSG00000069706
AA Change: L6H

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	45	320	8.3e-9	PFAM
Pfam:7tm_1	51	305	1.3e-54	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.0%
20x: 91.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal olfactory system physiology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003H04Rik	T	A	3: 124,372,124 (GRCm39)	H86L	possibly damaging	Het
4930519G04Rik	A	G	5: 115,008,278 (GRCm39)	T31A	probably benign	Het
Ahcyl1	A	G	3: 107,603,505 (GRCm39)	F30S	probably benign	Het
Alox5ap	G	A	5: 149,201,993 (GRCm39)		probably null	Het
Amtn	A	G	5: 88,526,729 (GRCm39)	S53G	probably null	Het
Arap3	A	T	18: 38,122,737 (GRCm39)		probably null	Het
Ash1l	T	A	3: 88,979,783 (GRCm39)	V2769E	probably damaging	Het
Atp8b1	C	T	18: 64,678,335 (GRCm39)	V854M	probably damaging	Het
Bhlha9	C	T	11: 76,563,457 (GRCm39)	S28L	probably benign	Het
Bltp2	T	A	11: 78,180,169 (GRCm39)	Y2150N	probably damaging	Het
Bmpr2	A	G	1: 59,907,285 (GRCm39)	T793A	probably benign	Het
Ccdc80	G	A	16: 44,916,299 (GRCm39)	A352T	probably benign	Het
Chst2	A	T	9: 95,288,194 (GRCm39)	F51I	probably benign	Het
Col14a1	A	G	15: 55,244,163 (GRCm39)	N412S	unknown	Het
Dclk2	T	C	3: 86,713,491 (GRCm39)	I451V	probably damaging	Het
Ddb2	A	G	2: 91,065,234 (GRCm39)	S64P	probably benign	Het
Diaph1	A	G	18: 38,029,146 (GRCm39)		probably null	Het
Dnai3	T	C	3: 145,748,504 (GRCm39)	E870G	probably damaging	Het
Dusp4	G	T	8: 35,285,570 (GRCm39)	R277L	probably benign	Het
Fmnl2	A	G	2: 52,995,549 (GRCm39)	E424G	probably damaging	Het
Garem1	A	T	18: 21,301,931 (GRCm39)		probably null	Het
Gnpat	A	G	8: 125,597,555 (GRCm39)	E39G	probably damaging	Het
Golgb1	A	T	16: 36,719,150 (GRCm39)	Q352L	possibly damaging	Het
Hdlbp	T	C	1: 93,345,096 (GRCm39)	D803G	probably benign	Het
Hps1	A	G	19: 42,748,143 (GRCm39)		probably null	Het
Ilvbl	G	A	10: 78,415,565 (GRCm39)	R327H	probably benign	Het
Itpr3	A	G	17: 27,333,121 (GRCm39)	D1911G	possibly damaging	Het
Lmo7	T	A	14: 102,166,700 (GRCm39)		probably benign	Het
Mcm10	G	A	2: 5,003,591 (GRCm39)	T542I	possibly damaging	Het
Med1	A	G	11: 98,051,772 (GRCm39)	V497A	probably damaging	Het
Mn1	G	A	5: 111,602,646 (GRCm39)	A1295T	probably damaging	Het
Myh7b	A	C	2: 155,473,707 (GRCm39)	D1580A	probably damaging	Het
Naa20	A	G	2: 145,754,438 (GRCm39)	I101V	probably benign	Het
Nav3	T	C	10: 109,702,846 (GRCm39)	Y229C	probably damaging	Het
Obscn	T	C	11: 58,891,575 (GRCm39)	R6986G	unknown	Het
Or51h7	G	A	7: 102,591,547 (GRCm39)	T79I	probably damaging	Het
Or8b4	A	G	9: 37,830,570 (GRCm39)	I211V	probably benign	Het
P2rx3	A	G	2: 84,853,825 (GRCm39)		probably null	Het
Pcdhac2	G	A	18: 37,279,539 (GRCm39)	G840R	possibly damaging	Het
Pclo	A	G	5: 14,762,489 (GRCm39)	N3654S	unknown	Het
Pcnx2	T	A	8: 126,604,188 (GRCm39)	E689D	possibly damaging	Het
Pds5a	A	G	5: 65,804,464 (GRCm39)	S532P	probably benign	Het
Phf1	G	A	17: 27,154,372 (GRCm39)		probably null	Het
Pkp4	G	A	2: 59,045,147 (GRCm39)	V41M	probably damaging	Het
Prlr	T	G	15: 10,328,364 (GRCm39)		probably null	Het
Prr12	G	T	7: 44,678,366 (GRCm39)	A1954D	unknown	Het
Prtg	A	G	9: 72,717,039 (GRCm39)	T127A	probably benign	Het
Ptprh	A	G	7: 4,552,698 (GRCm39)	L884P	probably damaging	Het
Rnf170	T	A	8: 26,629,076 (GRCm39)	D183E	probably benign	Het
Rrp12	A	T	19: 41,875,242 (GRCm39)	H339Q	probably damaging	Het
Rubcnl	T	G	14: 75,278,267 (GRCm39)	S350R	possibly damaging	Het
Sgo2b	T	A	8: 64,379,536 (GRCm39)	T1099S	possibly damaging	Het
Ska2	T	C	11: 87,006,945 (GRCm39)	S17P	probably damaging	Het
Slc38a2	A	G	15: 96,591,034 (GRCm39)	I243T	possibly damaging	Het
Sptan1	T	A	2: 29,916,034 (GRCm39)	D2007E	possibly damaging	Het
Tbata	G	T	10: 61,019,270 (GRCm39)		probably null	Het
Tent2	C	T	13: 93,312,076 (GRCm39)	G208D	probably damaging	Het
Tmem107	T	C	11: 68,963,284 (GRCm39)	S98P	probably damaging	Het
Tpst2	A	G	5: 112,456,286 (GRCm39)	D275G	possibly damaging	Het
Ttc28	G	T	5: 111,433,184 (GRCm39)	G2073W	probably damaging	Het
Ttc7	T	C	17: 87,629,891 (GRCm39)	V291A	possibly damaging	Het
Vps13b	T	A	15: 35,792,327 (GRCm39)	N2198K	possibly damaging	Het
Wdr37	A	T	13: 8,887,039 (GRCm39)	D249E	probably benign	Het
Xirp2	G	T	2: 67,340,357 (GRCm39)	C866F	probably damaging	Het
Zfp990	A	G	4: 145,263,566 (GRCm39)	E188G	possibly damaging	Het
Zkscan2	A	G	7: 123,099,064 (GRCm39)	S43P	possibly damaging	Het
Zscan5b	A	G	7: 6,236,850 (GRCm39)	R200G	probably benign	Het

Other mutations in Taar5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01602:Taar5	APN	10	23,846,962 (GRCm39)	missense	probably benign	0.06
IGL01605:Taar5	APN	10	23,846,962 (GRCm39)	missense	probably benign	0.06
IGL02935:Taar5	APN	10	23,847,415 (GRCm39)	missense	probably damaging	1.00
IGL02962:Taar5	APN	10	23,846,883 (GRCm39)	missense	possibly damaging	0.91
R0136:Taar5	UTSW	10	23,847,607 (GRCm39)	missense	probably damaging	1.00
R1216:Taar5	UTSW	10	23,847,605 (GRCm39)	missense	probably damaging	1.00
R2033:Taar5	UTSW	10	23,846,992 (GRCm39)	missense	possibly damaging	0.84
R2158:Taar5	UTSW	10	23,846,986 (GRCm39)	missense	probably damaging	1.00
R4914:Taar5	UTSW	10	23,847,468 (GRCm39)	missense	possibly damaging	0.90
R4999:Taar5	UTSW	10	23,847,445 (GRCm39)	missense	possibly damaging	0.88
R5084:Taar5	UTSW	10	23,846,836 (GRCm39)	missense	probably damaging	1.00
R6277:Taar5	UTSW	10	23,847,169 (GRCm39)	missense	probably damaging	1.00
R6516:Taar5	UTSW	10	23,847,564 (GRCm39)	missense	possibly damaging	0.89
R7974:Taar5	UTSW	10	23,847,120 (GRCm39)	missense	possibly damaging	0.95
R8147:Taar5	UTSW	10	23,846,749 (GRCm39)	missense	probably damaging	1.00
R8156:Taar5	UTSW	10	23,847,393 (GRCm39)	missense	probably damaging	1.00
R9306:Taar5	UTSW	10	23,846,781 (GRCm39)	missense	possibly damaging	0.95
Z1177:Taar5	UTSW	10	23,847,339 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCAGGGACCAGTCAGTTCCATCAC -3'
(R):5'- TCCAGATAGGTATGCAGACGGCAC -3'

Sequencing Primer
(F):5'- AGTCAGTTCCATCACCCTTTAC -3'
(R):5'- TAGCAACATGTCTGCCAGGG -3'

Posted On

2014-04-13