Mutagenetix > Incidental Mutation

Incidental Mutation 'R1549:Vmn2r12'

169776

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

039588-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R1549 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109233715-109245730 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 109240696 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Serine at position 139 (Y139S)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

probably benign
Transcript: ENSMUST00000095922
AA Change: Y139S

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: Y139S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.8%
20x: 90.8%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	A	T	3: 124,210,441 (GRCm39)	I157K	probably benign	Het
Ank3	A	T	10: 69,837,812 (GRCm39)	N727Y	probably benign	Het
Atg16l1	A	G	1: 87,701,910 (GRCm39)	T251A	probably benign	Het
Bcl11b	T	A	12: 107,883,422 (GRCm39)	I226F	probably damaging	Het
Birc6	A	G	17: 74,969,737 (GRCm39)	H4260R	probably damaging	Het
Camta1	A	G	4: 151,670,920 (GRCm39)	I85T	probably damaging	Het
Cblb	A	G	16: 51,853,373 (GRCm39)		probably benign	Het
Ccnd1	T	G	7: 144,491,073 (GRCm39)	I178L	probably benign	Het
Clcn4	A	G	7: 7,294,681 (GRCm39)	V329A	probably damaging	Het
Col17a1	C	T	19: 47,637,349 (GRCm39)		probably benign	Het
Col7a1	A	G	9: 108,785,034 (GRCm39)	T254A	unknown	Het
Ctif	C	A	18: 75,698,096 (GRCm39)	R188L	probably damaging	Het
Cyp2c69	A	T	19: 39,831,430 (GRCm39)	L461Q	probably benign	Het
Ddc	A	G	11: 11,796,656 (GRCm39)		probably null	Het
Dpp10	T	A	1: 123,269,109 (GRCm39)		probably null	Het
Eef2	CCC	CCCC	10: 81,014,602 (GRCm39)		probably null	Het
Eif2b4	T	C	5: 31,350,265 (GRCm39)	E19G	possibly damaging	Het
Fmnl2	A	G	2: 52,995,549 (GRCm39)	E424G	probably damaging	Het
Galnt14	A	G	17: 73,832,308 (GRCm39)	L269P	possibly damaging	Het
Gm2381	T	A	7: 42,471,825 (GRCm39)	H18L	probably benign	Het
Gpbp1	G	T	13: 111,573,113 (GRCm39)	D326E	probably benign	Het
Gpr162	C	T	6: 124,837,051 (GRCm39)	R331H	probably damaging	Het
Iigp1	G	A	18: 60,522,948 (GRCm39)	G22D	probably benign	Het
Kcns3	T	C	12: 11,142,084 (GRCm39)	H205R	probably damaging	Het
Klk1b26	C	T	7: 43,665,826 (GRCm39)		probably benign	Het
Lime1	A	G	2: 181,025,169 (GRCm39)	Y242C	probably benign	Het
Manba	A	G	3: 135,250,567 (GRCm39)	D398G	probably damaging	Het
Mapk3	A	T	7: 126,362,684 (GRCm39)	K219*	probably null	Het
Mdfic	G	T	6: 15,799,844 (GRCm39)	G324C	probably damaging	Het
Mdga1	G	A	17: 30,056,972 (GRCm39)	H837Y	probably damaging	Het
Nck1	T	A	9: 100,379,925 (GRCm39)	M109L	probably benign	Het
Or1d2	A	G	11: 74,256,076 (GRCm39)	I194V	probably benign	Het
Or2w1b	T	C	13: 21,300,288 (GRCm39)	V142A	probably benign	Het
Pabpc1l	G	T	2: 163,879,091 (GRCm39)	V313F	possibly damaging	Het
Phf3	C	T	1: 30,843,923 (GRCm39)	V1679I	probably benign	Het
Pou4f1	T	C	14: 104,705,076 (GRCm39)	I32V	probably benign	Het
Pspc1	T	G	14: 56,986,398 (GRCm39)	H351P	probably damaging	Het
Ptk7	T	C	17: 46,883,578 (GRCm39)	E829G	probably damaging	Het
Rab23	A	G	1: 33,777,378 (GRCm39)	Y164C	possibly damaging	Het
Slc36a3	A	G	11: 55,033,596 (GRCm39)	W141R	probably damaging	Het
Slc7a14	T	C	3: 31,278,267 (GRCm39)	E446G	possibly damaging	Het
Stim2	G	A	5: 54,262,667 (GRCm39)	R303Q	probably damaging	Het
Tbcd	A	G	11: 121,451,579 (GRCm39)	I550V	probably benign	Het
Tmed3	A	C	9: 89,581,998 (GRCm39)	L155R	probably damaging	Het
Trav16d-dv11	A	G	14: 53,284,799 (GRCm39)		probably benign	Het
Ubap2	C	T	4: 41,199,872 (GRCm39)	A752T	probably benign	Het
Usp16	G	T	16: 87,261,722 (GRCm39)	V113F	probably damaging	Het
Vmn2r5	G	T	3: 64,411,421 (GRCm39)	D382E	probably damaging	Het
Zfp1010	T	C	2: 176,957,378 (GRCm39)	E40G	probably damaging	Het
Zfp217	A	C	2: 169,956,390 (GRCm39)	N869K	probably benign	Het
Zswim2	G	T	2: 83,754,092 (GRCm39)	D189E	probably benign	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109,245,541 (GRCm39)	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109,234,125 (GRCm39)	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109,239,716 (GRCm39)	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109,240,893 (GRCm39)	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109,240,025 (GRCm39)	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109,234,343 (GRCm39)	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109,233,858 (GRCm39)	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109,238,351 (GRCm39)	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109,239,936 (GRCm39)	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109,240,765 (GRCm39)	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109,239,755 (GRCm39)	nonsense	probably null
R0529:Vmn2r12	UTSW	5	109,240,714 (GRCm39)	missense	probably benign
R0715:Vmn2r12	UTSW	5	109,238,373 (GRCm39)	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109,234,281 (GRCm39)	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109,235,716 (GRCm39)	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109,239,763 (GRCm39)	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109,240,840 (GRCm39)	missense	possibly damaging	0.56
R1766:Vmn2r12	UTSW	5	109,239,910 (GRCm39)	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109,239,594 (GRCm39)	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109,239,942 (GRCm39)	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109,239,340 (GRCm39)	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109,238,370 (GRCm39)	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109,239,412 (GRCm39)	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109,234,301 (GRCm39)	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109,234,379 (GRCm39)	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109,240,852 (GRCm39)	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109,239,544 (GRCm39)	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109,239,372 (GRCm39)	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109,238,261 (GRCm39)	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109,239,684 (GRCm39)	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109,234,483 (GRCm39)	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109,240,666 (GRCm39)	missense	probably benign	0.06
R5753:Vmn2r12	UTSW	5	109,239,670 (GRCm39)	missense	probably damaging	1.00
R5797:Vmn2r12	UTSW	5	109,233,736 (GRCm39)	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109,240,763 (GRCm39)	missense	probably benign
R6162:Vmn2r12	UTSW	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109,233,866 (GRCm39)	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109,240,771 (GRCm39)	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109,245,655 (GRCm39)	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109,239,811 (GRCm39)	missense	possibly damaging	0.95
R7341:Vmn2r12	UTSW	5	109,234,113 (GRCm39)	missense	possibly damaging	0.74
R7383:Vmn2r12	UTSW	5	109,240,684 (GRCm39)	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109,239,615 (GRCm39)	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109,233,920 (GRCm39)	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109,235,829 (GRCm39)	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109,234,307 (GRCm39)	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109,239,747 (GRCm39)	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109,238,349 (GRCm39)	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109,234,074 (GRCm39)	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109,239,952 (GRCm39)	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109,234,522 (GRCm39)	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109,234,033 (GRCm39)	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109,233,885 (GRCm39)	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109,240,910 (GRCm39)	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109,234,203 (GRCm39)	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109,233,986 (GRCm39)	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109,239,823 (GRCm39)	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109,239,322 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109,240,646 (GRCm39)	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109,239,303 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTGTTAACTTgggctatcgagctgg -3'
(R):5'- TGGCAGCACTCTGACTCTCCAC -3'

Sequencing Primer
(F):5'- tcctaaattcaattcccaataaccac -3'
(R):5'- CTAGTTCAAGGTTAGCATTCAGAG -3'

Posted On

2014-04-13