Mutagenetix > Incidental Mutation

Incidental Mutation 'R1549:Or2w1b'

169798

Institutional Source

Beutler Lab

Gene Symbol

Or2w1b

Ensembl Gene

ENSMUSG00000060404

Gene Name

olfactory receptor family 2 subfamily W member 1B

Synonyms

Olfr1369, MOR256-31, GA_x6K02T2QHY8-12126170-12125935

MMRRC Submission

039588-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.346) question?

Stock #

R1549 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21299764-21300871 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21300288 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 142 (V142A)

Ref Sequence

ENSEMBL: ENSMUSP00000151041 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079050] [ENSMUST00000213326] [ENSMUST00000213922] [ENSMUST00000215207] [ENSMUST00000215941]

AlphaFold

A0A140T8K7

Predicted Effect

probably benign
Transcript: ENSMUST00000079050
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000078059
Gene: ENSMUSG00000060404
AA Change: V142A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	6.2e-47	PFAM
Pfam:7tm_1	41	290	1.1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213326
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000213922
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000215207
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000215941
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.8%
20x: 90.8%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	A	T	3: 124,210,441 (GRCm39)	I157K	probably benign	Het
Ank3	A	T	10: 69,837,812 (GRCm39)	N727Y	probably benign	Het
Atg16l1	A	G	1: 87,701,910 (GRCm39)	T251A	probably benign	Het
Bcl11b	T	A	12: 107,883,422 (GRCm39)	I226F	probably damaging	Het
Birc6	A	G	17: 74,969,737 (GRCm39)	H4260R	probably damaging	Het
Camta1	A	G	4: 151,670,920 (GRCm39)	I85T	probably damaging	Het
Cblb	A	G	16: 51,853,373 (GRCm39)		probably benign	Het
Ccnd1	T	G	7: 144,491,073 (GRCm39)	I178L	probably benign	Het
Clcn4	A	G	7: 7,294,681 (GRCm39)	V329A	probably damaging	Het
Col17a1	C	T	19: 47,637,349 (GRCm39)		probably benign	Het
Col7a1	A	G	9: 108,785,034 (GRCm39)	T254A	unknown	Het
Ctif	C	A	18: 75,698,096 (GRCm39)	R188L	probably damaging	Het
Cyp2c69	A	T	19: 39,831,430 (GRCm39)	L461Q	probably benign	Het
Ddc	A	G	11: 11,796,656 (GRCm39)		probably null	Het
Dpp10	T	A	1: 123,269,109 (GRCm39)		probably null	Het
Eef2	CCC	CCCC	10: 81,014,602 (GRCm39)		probably null	Het
Eif2b4	T	C	5: 31,350,265 (GRCm39)	E19G	possibly damaging	Het
Fmnl2	A	G	2: 52,995,549 (GRCm39)	E424G	probably damaging	Het
Galnt14	A	G	17: 73,832,308 (GRCm39)	L269P	possibly damaging	Het
Gm2381	T	A	7: 42,471,825 (GRCm39)	H18L	probably benign	Het
Gpbp1	G	T	13: 111,573,113 (GRCm39)	D326E	probably benign	Het
Gpr162	C	T	6: 124,837,051 (GRCm39)	R331H	probably damaging	Het
Iigp1	G	A	18: 60,522,948 (GRCm39)	G22D	probably benign	Het
Kcns3	T	C	12: 11,142,084 (GRCm39)	H205R	probably damaging	Het
Klk1b26	C	T	7: 43,665,826 (GRCm39)		probably benign	Het
Lime1	A	G	2: 181,025,169 (GRCm39)	Y242C	probably benign	Het
Manba	A	G	3: 135,250,567 (GRCm39)	D398G	probably damaging	Het
Mapk3	A	T	7: 126,362,684 (GRCm39)	K219*	probably null	Het
Mdfic	G	T	6: 15,799,844 (GRCm39)	G324C	probably damaging	Het
Mdga1	G	A	17: 30,056,972 (GRCm39)	H837Y	probably damaging	Het
Nck1	T	A	9: 100,379,925 (GRCm39)	M109L	probably benign	Het
Or1d2	A	G	11: 74,256,076 (GRCm39)	I194V	probably benign	Het
Pabpc1l	G	T	2: 163,879,091 (GRCm39)	V313F	possibly damaging	Het
Phf3	C	T	1: 30,843,923 (GRCm39)	V1679I	probably benign	Het
Pou4f1	T	C	14: 104,705,076 (GRCm39)	I32V	probably benign	Het
Pspc1	T	G	14: 56,986,398 (GRCm39)	H351P	probably damaging	Het
Ptk7	T	C	17: 46,883,578 (GRCm39)	E829G	probably damaging	Het
Rab23	A	G	1: 33,777,378 (GRCm39)	Y164C	possibly damaging	Het
Slc36a3	A	G	11: 55,033,596 (GRCm39)	W141R	probably damaging	Het
Slc7a14	T	C	3: 31,278,267 (GRCm39)	E446G	possibly damaging	Het
Stim2	G	A	5: 54,262,667 (GRCm39)	R303Q	probably damaging	Het
Tbcd	A	G	11: 121,451,579 (GRCm39)	I550V	probably benign	Het
Tmed3	A	C	9: 89,581,998 (GRCm39)	L155R	probably damaging	Het
Trav16d-dv11	A	G	14: 53,284,799 (GRCm39)		probably benign	Het
Ubap2	C	T	4: 41,199,872 (GRCm39)	A752T	probably benign	Het
Usp16	G	T	16: 87,261,722 (GRCm39)	V113F	probably damaging	Het
Vmn2r12	T	G	5: 109,240,696 (GRCm39)	Y139S	probably benign	Het
Vmn2r5	G	T	3: 64,411,421 (GRCm39)	D382E	probably damaging	Het
Zfp1010	T	C	2: 176,957,378 (GRCm39)	E40G	probably damaging	Het
Zfp217	A	C	2: 169,956,390 (GRCm39)	N869K	probably benign	Het
Zswim2	G	T	2: 83,754,092 (GRCm39)	D189E	probably benign	Het

Other mutations in Or2w1b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01689:Or2w1b	APN	13	21,300,243 (GRCm39)	missense	probably damaging	0.97
R0631:Or2w1b	UTSW	13	21,300,078 (GRCm39)	missense	probably damaging	1.00
R0960:Or2w1b	UTSW	13	21,300,435 (GRCm39)	missense	possibly damaging	0.94
R1499:Or2w1b	UTSW	13	21,300,303 (GRCm39)	missense	probably benign	0.32
R1698:Or2w1b	UTSW	13	21,300,735 (GRCm39)	missense	probably benign	0.11
R1711:Or2w1b	UTSW	13	21,300,476 (GRCm39)	missense	probably benign	0.01
R2404:Or2w1b	UTSW	13	21,300,012 (GRCm39)	missense	probably damaging	1.00
R2471:Or2w1b	UTSW	13	21,300,599 (GRCm39)	missense	probably damaging	1.00
R3844:Or2w1b	UTSW	13	21,300,233 (GRCm39)	missense	possibly damaging	0.91
R3977:Or2w1b	UTSW	13	21,300,031 (GRCm39)	missense	probably benign	0.03
R3979:Or2w1b	UTSW	13	21,300,031 (GRCm39)	missense	probably benign	0.03
R4804:Or2w1b	UTSW	13	21,300,175 (GRCm39)	nonsense	probably null
R4914:Or2w1b	UTSW	13	21,300,567 (GRCm39)	missense	probably benign	0.12
R5210:Or2w1b	UTSW	13	21,300,222 (GRCm39)	missense	probably damaging	0.99
R5359:Or2w1b	UTSW	13	21,300,437 (GRCm39)	missense	probably damaging	1.00
R5700:Or2w1b	UTSW	13	21,300,171 (GRCm39)	missense	probably damaging	1.00
R6218:Or2w1b	UTSW	13	21,300,401 (GRCm39)	missense	probably damaging	1.00
R6767:Or2w1b	UTSW	13	21,300,227 (GRCm39)	missense	probably benign	0.02
R7396:Or2w1b	UTSW	13	21,300,477 (GRCm39)	missense	probably benign	0.02
R7476:Or2w1b	UTSW	13	21,300,191 (GRCm39)	missense	probably benign	0.04
R7612:Or2w1b	UTSW	13	21,300,217 (GRCm39)	missense	probably damaging	0.99
R8257:Or2w1b	UTSW	13	21,300,543 (GRCm39)	missense	probably benign	0.11
R9388:Or2w1b	UTSW	13	21,300,774 (GRCm39)	missense	probably damaging	0.96
R9697:Or2w1b	UTSW	13	21,299,892 (GRCm39)	missense	probably benign	0.21
V8831:Or2w1b	UTSW	13	21,300,173 (GRCm39)	missense	possibly damaging	0.93
Z1176:Or2w1b	UTSW	13	21,300,771 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGACCCGAAAAGACCATTAGCTCTG -3'
(R):5'- CTGCCTTTGACTTCATGTTGAGCAC -3'

Sequencing Primer
(F):5'- CATTAGCTCTGTGGGCTGTATTG -3'
(R):5'- ACTTCATGTTGAGCACAGTTTTG -3'

Posted On

2014-04-13