Incidental Mutation 'R1552:Dclk3'
| ID |
170022 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Dclk3
|
| Ensembl Gene |
ENSMUSG00000032500 |
| Gene Name |
doublecortin-like kinase 3 |
| Synonyms |
Dcamkl3, Click-I, -II related |
| MMRRC Submission |
039591-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.189)
|
| Stock # |
R1552 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
9 |
| Chromosomal Location |
111268149-111318186 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to A
at 111317647 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Lysine
at position 761
(T761K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000107510
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000111879]
|
| AlphaFold |
Q8BWQ5 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000111879
AA Change: T761K
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: T761K
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
2 |
29 |
N/A |
INTRINSIC |
|
Pfam:DCX
|
115 |
177 |
2.9e-17 |
PFAM |
|
low complexity region
|
200 |
218 |
N/A |
INTRINSIC |
|
low complexity region
|
267 |
277 |
N/A |
INTRINSIC |
|
low complexity region
|
479 |
496 |
N/A |
INTRINSIC |
|
S_TKc
|
514 |
771 |
1.63e-110 |
SMART |
|
| Meta Mutation Damage Score |
0.3703 |
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.3%
- 10x: 96.1%
- 20x: 91.7%
|
| Validation Efficiency |
97% (71/73) |
| MGI Phenotype |
FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]
|
| Allele List at MGI |
All alleles(3) : Targeted, other(2) Gene trapped(1) |
| Other mutations in this stock |
Total: 68 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcc5 |
T |
A |
16: 20,217,617 (GRCm39) |
I365F |
probably damaging |
Het |
| Abhd15 |
T |
C |
11: 77,406,233 (GRCm39) |
L70P |
probably damaging |
Het |
| Adam18 |
A |
C |
8: 25,136,377 (GRCm39) |
H381Q |
probably benign |
Het |
| Ankfy1 |
T |
C |
11: 72,645,321 (GRCm39) |
|
probably null |
Het |
| Arcn1 |
C |
T |
9: 44,670,291 (GRCm39) |
A112T |
probably damaging |
Het |
| Bltp3a |
T |
C |
17: 28,109,045 (GRCm39) |
F1088S |
possibly damaging |
Het |
| Calhm1 |
C |
T |
19: 47,129,640 (GRCm39) |
R294H |
probably benign |
Het |
| Ccdc121rt1 |
A |
G |
1: 181,338,556 (GRCm39) |
L132P |
probably damaging |
Het |
| Cdk5rap1 |
T |
C |
2: 154,212,615 (GRCm39) |
E81G |
probably benign |
Het |
| Cep170 |
T |
C |
1: 176,610,060 (GRCm39) |
|
probably benign |
Het |
| Cep350 |
C |
T |
1: 155,786,484 (GRCm39) |
R1454Q |
possibly damaging |
Het |
| Chrna3 |
C |
A |
9: 54,923,192 (GRCm39) |
E205D |
probably benign |
Het |
| Chst5 |
T |
C |
8: 112,616,912 (GRCm39) |
D236G |
probably damaging |
Het |
| Coro1a |
T |
C |
7: 126,299,124 (GRCm39) |
N367D |
probably benign |
Het |
| Cyp3a16 |
T |
C |
5: 145,373,346 (GRCm39) |
I474V |
probably benign |
Het |
| Cyth3 |
G |
A |
5: 143,683,505 (GRCm39) |
V87I |
probably benign |
Het |
| Dvl2 |
T |
A |
11: 69,897,198 (GRCm39) |
M300K |
possibly damaging |
Het |
| Eefsec |
C |
T |
6: 88,353,182 (GRCm39) |
|
probably benign |
Het |
| Exog |
T |
C |
9: 119,274,176 (GRCm39) |
S54P |
unknown |
Het |
| Fasn |
A |
G |
11: 120,709,384 (GRCm39) |
S519P |
probably damaging |
Het |
| Gas8 |
G |
A |
8: 124,247,385 (GRCm39) |
A16T |
probably benign |
Het |
| Got2 |
A |
G |
8: 96,596,122 (GRCm39) |
S333P |
probably benign |
Het |
| Hadhb |
T |
A |
5: 30,381,931 (GRCm39) |
L287Q |
probably null |
Het |
| Ift57 |
A |
G |
16: 49,579,716 (GRCm39) |
T211A |
probably benign |
Het |
| Il1rap |
A |
G |
16: 26,541,184 (GRCm39) |
E475G |
possibly damaging |
Het |
| Ilkap |
A |
T |
1: 91,312,316 (GRCm39) |
D11E |
probably damaging |
Het |
| Impact |
T |
C |
18: 13,117,337 (GRCm39) |
S137P |
probably benign |
Het |
| Jarid2 |
T |
C |
13: 45,064,675 (GRCm39) |
V920A |
probably damaging |
Het |
| Kcnk18 |
A |
T |
19: 59,223,890 (GRCm39) |
H345L |
probably damaging |
Het |
| Kdm4d |
A |
T |
9: 14,375,325 (GRCm39) |
Y178N |
probably damaging |
Het |
| Klk1b1 |
A |
G |
7: 43,618,767 (GRCm39) |
Y48C |
probably damaging |
Het |
| Klra5 |
G |
T |
6: 129,886,848 (GRCm39) |
T60K |
probably damaging |
Het |
| Kng2 |
A |
T |
16: 22,806,270 (GRCm39) |
L643H |
probably damaging |
Het |
| Lamb3 |
A |
G |
1: 193,013,067 (GRCm39) |
|
probably null |
Het |
| Lingo2 |
A |
G |
4: 35,708,315 (GRCm39) |
V555A |
probably damaging |
Het |
| Mbd5 |
T |
C |
2: 49,162,946 (GRCm39) |
S251P |
probably damaging |
Het |
| Mc4r |
T |
C |
18: 66,992,766 (GRCm39) |
S116G |
probably benign |
Het |
| Mipol1 |
T |
C |
12: 57,352,874 (GRCm39) |
V71A |
possibly damaging |
Het |
| Myo15b |
A |
C |
11: 115,757,461 (GRCm39) |
S1104R |
probably benign |
Het |
| Nek1 |
T |
A |
8: 61,459,771 (GRCm39) |
D26E |
probably damaging |
Het |
| Neu1 |
C |
T |
17: 35,151,089 (GRCm39) |
|
probably benign |
Het |
| Npffr2 |
T |
C |
5: 89,730,975 (GRCm39) |
S302P |
possibly damaging |
Het |
| Or8b48 |
T |
C |
9: 38,492,675 (GRCm39) |
M34T |
probably benign |
Het |
| Palmd |
T |
A |
3: 116,741,689 (GRCm39) |
|
probably benign |
Het |
| Pcdh8 |
A |
T |
14: 80,008,047 (GRCm39) |
V172E |
probably benign |
Het |
| Pnpla6 |
C |
A |
8: 3,572,403 (GRCm39) |
Q291K |
probably damaging |
Het |
| Pou2af2 |
T |
A |
9: 51,202,870 (GRCm39) |
S95C |
probably damaging |
Het |
| Prkch |
T |
C |
12: 73,749,320 (GRCm39) |
F357L |
probably benign |
Het |
| Ptprj |
A |
T |
2: 90,301,497 (GRCm39) |
Y212N |
probably damaging |
Het |
| Reln |
G |
T |
5: 22,165,376 (GRCm39) |
H2061N |
probably benign |
Het |
| Rint1 |
T |
A |
5: 24,005,656 (GRCm39) |
S113T |
probably benign |
Het |
| Rnf38 |
G |
C |
4: 44,142,468 (GRCm39) |
|
probably null |
Het |
| Slc30a4 |
T |
A |
2: 122,527,936 (GRCm39) |
I374L |
probably benign |
Het |
| Slfn10-ps |
T |
C |
11: 82,920,676 (GRCm39) |
|
noncoding transcript |
Het |
| Smcp |
A |
T |
3: 92,491,710 (GRCm39) |
C46S |
unknown |
Het |
| Smu1 |
A |
C |
4: 40,748,570 (GRCm39) |
V240G |
probably damaging |
Het |
| Srsf5 |
A |
G |
12: 80,996,519 (GRCm39) |
|
probably benign |
Het |
| Stn1 |
T |
C |
19: 47,524,812 (GRCm39) |
|
probably null |
Het |
| Stx18 |
A |
G |
5: 38,262,335 (GRCm39) |
E63G |
probably damaging |
Het |
| Tas2r130 |
A |
G |
6: 131,607,130 (GRCm39) |
Y222H |
probably benign |
Het |
| Tescl |
G |
T |
7: 24,032,758 (GRCm39) |
P189Q |
probably benign |
Het |
| Tlr1 |
A |
G |
5: 65,084,203 (GRCm39) |
S125P |
probably damaging |
Het |
| Ugt2a2 |
G |
T |
5: 87,609,880 (GRCm39) |
D566E |
possibly damaging |
Het |
| Unc13b |
A |
T |
4: 43,237,144 (GRCm39) |
T3405S |
probably damaging |
Het |
| Upf1 |
G |
A |
8: 70,785,709 (GRCm39) |
Q1046* |
probably null |
Het |
| Wwox |
T |
A |
8: 115,172,090 (GRCm39) |
Y61* |
probably null |
Het |
| Zfhx4 |
C |
T |
3: 5,468,170 (GRCm39) |
T2776M |
probably damaging |
Het |
| Zranb3 |
G |
A |
1: 127,888,488 (GRCm39) |
|
probably benign |
Het |
|
| Other mutations in Dclk3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02027:Dclk3
|
APN |
9 |
111,296,911 (GRCm39) |
nonsense |
probably null |
|
|
IGL02125:Dclk3
|
APN |
9 |
111,298,175 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02547:Dclk3
|
APN |
9 |
111,298,091 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03393:Dclk3
|
APN |
9 |
111,317,741 (GRCm39) |
utr 3 prime |
probably benign |
|
|
G1citation:Dclk3
|
UTSW |
9 |
111,268,405 (GRCm39) |
missense |
probably benign |
0.03 |
|
IGL02984:Dclk3
|
UTSW |
9 |
111,317,643 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0109:Dclk3
|
UTSW |
9 |
111,296,738 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R0109:Dclk3
|
UTSW |
9 |
111,296,738 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R0238:Dclk3
|
UTSW |
9 |
111,311,696 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0238:Dclk3
|
UTSW |
9 |
111,311,696 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0432:Dclk3
|
UTSW |
9 |
111,314,003 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0440:Dclk3
|
UTSW |
9 |
111,298,231 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0530:Dclk3
|
UTSW |
9 |
111,311,789 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1024:Dclk3
|
UTSW |
9 |
111,298,138 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R1443:Dclk3
|
UTSW |
9 |
111,298,088 (GRCm39) |
missense |
probably benign |
0.01 |
|
R1474:Dclk3
|
UTSW |
9 |
111,298,304 (GRCm39) |
missense |
probably benign |
0.43 |
|
R1479:Dclk3
|
UTSW |
9 |
111,297,614 (GRCm39) |
missense |
probably benign |
|
|
R1482:Dclk3
|
UTSW |
9 |
111,296,888 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R1543:Dclk3
|
UTSW |
9 |
111,297,122 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1559:Dclk3
|
UTSW |
9 |
111,298,276 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2011:Dclk3
|
UTSW |
9 |
111,297,422 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2369:Dclk3
|
UTSW |
9 |
111,317,610 (GRCm39) |
missense |
probably benign |
0.16 |
|
R4111:Dclk3
|
UTSW |
9 |
111,298,148 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4510:Dclk3
|
UTSW |
9 |
111,297,060 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4511:Dclk3
|
UTSW |
9 |
111,297,060 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4592:Dclk3
|
UTSW |
9 |
111,296,963 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4604:Dclk3
|
UTSW |
9 |
111,298,253 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4857:Dclk3
|
UTSW |
9 |
111,297,716 (GRCm39) |
missense |
probably benign |
|
|
R4932:Dclk3
|
UTSW |
9 |
111,297,110 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R5045:Dclk3
|
UTSW |
9 |
111,296,856 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5233:Dclk3
|
UTSW |
9 |
111,297,749 (GRCm39) |
missense |
probably benign |
|
|
R5338:Dclk3
|
UTSW |
9 |
111,298,127 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5463:Dclk3
|
UTSW |
9 |
111,298,328 (GRCm39) |
missense |
probably benign |
0.26 |
|
R6822:Dclk3
|
UTSW |
9 |
111,268,405 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6995:Dclk3
|
UTSW |
9 |
111,296,768 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R7187:Dclk3
|
UTSW |
9 |
111,314,064 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7532:Dclk3
|
UTSW |
9 |
111,296,596 (GRCm39) |
missense |
probably benign |
0.03 |
|
R7534:Dclk3
|
UTSW |
9 |
111,297,286 (GRCm39) |
missense |
probably benign |
|
|
R7734:Dclk3
|
UTSW |
9 |
111,298,163 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8326:Dclk3
|
UTSW |
9 |
111,296,602 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R8372:Dclk3
|
UTSW |
9 |
111,314,081 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8388:Dclk3
|
UTSW |
9 |
111,311,813 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8423:Dclk3
|
UTSW |
9 |
111,297,787 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R8493:Dclk3
|
UTSW |
9 |
111,297,215 (GRCm39) |
missense |
probably benign |
0.06 |
|
R9013:Dclk3
|
UTSW |
9 |
111,297,566 (GRCm39) |
missense |
probably benign |
0.31 |
|
R9114:Dclk3
|
UTSW |
9 |
111,317,683 (GRCm39) |
missense |
probably benign |
0.06 |
|
R9338:Dclk3
|
UTSW |
9 |
111,268,373 (GRCm39) |
missense |
unknown |
|
|
R9412:Dclk3
|
UTSW |
9 |
111,311,819 (GRCm39) |
critical splice donor site |
probably null |
|
|
R9701:Dclk3
|
UTSW |
9 |
111,298,244 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9776:Dclk3
|
UTSW |
9 |
111,298,226 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9802:Dclk3
|
UTSW |
9 |
111,298,244 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0020:Dclk3
|
UTSW |
9 |
111,314,143 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TCAGGTTGCCCTGGACAAGGAAAG -3'
(R):5'- TCTTGCACAGCCTTGCTTGTAGAC -3'
Sequencing Primer
(F):5'- CAAGGAAAGCTTATTAGTCCTTCCC -3'
(R):5'- ACAGCCTTGCTTGTAGACTAGAC -3'
|
| Posted On |
2014-04-13 |
Incidental Mutation