Mutagenetix > Incidental Mutation

Incidental Mutation 'R1573:Mki67'

170968

Institutional Source

Beutler Lab

Gene Symbol

Mki67

Ensembl Gene

ENSMUSG00000031004

Gene Name

antigen identified by monoclonal antibody Ki 67

Synonyms

D630048A14Rik, Ki-67, Ki67

MMRRC Submission

039612-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.832) question?

Stock #

R1573 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

135291513-135318090 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 135296845 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 2730 (P2730S)

Ref Sequence

ENSEMBL: ENSMUSP00000033310 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033310]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000033310
AA Change: P2730S

PolyPhen 2 Score 0.932 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000033310
Gene: ENSMUSG00000031004
AA Change: P2730S

Domain	Start	End	E-Value	Type
FHA	26	76	1.03e-11	SMART
Pfam:PP1_bind	462	519	2.8e-20	PFAM
low complexity region	535	545	N/A	INTRINSIC
low complexity region	869	882	N/A	INTRINSIC
Pfam:K167R	889	982	1.5e-9	PFAM
K167R	993	1102	2.01e-39	SMART
K167R	1107	1217	1.87e-57	SMART
K167R	1228	1337	1.33e-53	SMART
K167R	1348	1451	6.57e-44	SMART
K167R	1462	1570	9.09e-38	SMART
K167R	1580	1686	5.02e-40	SMART
K167R	1697	1807	9.6e-37	SMART
K167R	1818	1926	5.94e-51	SMART
K167R	1937	2047	1.6e-56	SMART
K167R	2058	2164	4.04e-53	SMART
K167R	2175	2285	1.52e-57	SMART
K167R	2296	2407	1.78e-40	SMART
K167R	2418	2527	1.71e-42	SMART
K167R	2538	2640	7.41e-20	SMART
K167R	2642	2750	1.06e-38	SMART
K167R	2761	2872	2.1e-42	SMART

Meta Mutation Damage Score

0.2198

Coding Region Coverage

1x: 98.9%
3x: 97.8%
10x: 94.6%
20x: 87.1%

Validation Efficiency

98% (90/92)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
PHENOTYPE: Mice carrying a reporter allele show expression in actively dividing cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 82 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam19	T	C	11: 46,004,445 (GRCm39)		probably benign	Het
Add1	C	G	5: 34,758,740 (GRCm39)	A18G	possibly damaging	Het
Alk	T	C	17: 72,910,113 (GRCm39)	K198E	possibly damaging	Het
Angptl1	T	A	1: 156,684,740 (GRCm39)	L303Q	possibly damaging	Het
Aox1	A	T	1: 58,348,186 (GRCm39)	I635L	probably benign	Het
Atp5mk	T	A	19: 47,074,634 (GRCm39)	Q9L	possibly damaging	Het
Atp8a2	G	T	14: 60,097,655 (GRCm39)	T791K	probably benign	Het
Auts2	T	C	5: 131,469,325 (GRCm39)	K664R	probably damaging	Het
Birc6	T	A	17: 74,967,685 (GRCm39)		probably benign	Het
Cacna1i	C	A	15: 80,277,869 (GRCm39)		probably null	Het
Cacna2d1	T	C	5: 16,575,625 (GRCm39)	F1077L	probably damaging	Het
Camkk1	C	T	11: 72,918,307 (GRCm39)	R52C	probably damaging	Het
Camkmt	T	A	17: 85,403,958 (GRCm39)	V60E	probably damaging	Het
Car9	G	T	4: 43,512,439 (GRCm39)		probably null	Het
Cbr2	A	T	11: 120,622,791 (GRCm39)	L3Q	possibly damaging	Het
Ccar1	A	T	10: 62,586,434 (GRCm39)	D920E	unknown	Het
Cdc20b	A	G	13: 113,192,478 (GRCm39)	N57S	probably benign	Het
Cep83	G	A	10: 94,624,525 (GRCm39)	E601K	probably damaging	Het
Cldn10	A	T	14: 119,111,080 (GRCm39)	I176L	probably benign	Het
Cpeb2	T	C	5: 43,441,273 (GRCm39)		probably benign	Het
Crb1	A	G	1: 139,265,344 (GRCm39)	S25P	probably damaging	Het
Cyp3a59	A	T	5: 146,039,684 (GRCm39)	Y319F	probably damaging	Het
Dst	C	T	1: 34,240,312 (GRCm39)	S1561F	probably damaging	Het
Dxo	C	T	17: 35,057,270 (GRCm39)	R221C	probably damaging	Het
Dynlt4	A	T	4: 116,985,191 (GRCm39)	T5S	probably benign	Het
Epc2	A	G	2: 49,439,984 (GRCm39)	T801A	possibly damaging	Het
Fndc3a	C	A	14: 72,806,384 (GRCm39)	C373F	probably damaging	Het
Frmpd1	T	C	4: 45,283,932 (GRCm39)	S918P	probably benign	Het
Fuca2	A	G	10: 13,381,587 (GRCm39)	T84A	possibly damaging	Het
Garin4	G	A	1: 190,896,682 (GRCm39)		probably benign	Het
Gmeb1	A	T	4: 131,979,051 (GRCm39)	N21K	probably benign	Het
Htt	T	C	5: 35,021,718 (GRCm39)		probably benign	Het
Igsf1	T	C	X: 48,880,863 (GRCm39)	R251G	possibly damaging	Het
Itih4	A	T	14: 30,619,504 (GRCm39)	H720L	probably benign	Het
Kank4	A	T	4: 98,663,073 (GRCm39)	L705*	probably null	Het
Krt34	A	T	11: 99,931,854 (GRCm39)	S122T	probably benign	Het
L1td1	A	G	4: 98,625,517 (GRCm39)	T571A	probably benign	Het
Lag3	T	C	6: 124,886,210 (GRCm39)	T248A	possibly damaging	Het
Lgi1	T	A	19: 38,272,629 (GRCm39)	H133Q	probably benign	Het
Map1a	A	T	2: 121,134,607 (GRCm39)	T1808S	probably benign	Het
Mcm9	T	C	10: 53,424,752 (GRCm39)	T613A	probably damaging	Het
Meaf6	A	G	4: 124,983,931 (GRCm39)	I111V	probably benign	Het
Mlc1	A	C	15: 88,842,350 (GRCm39)	C337G	probably damaging	Het
Mrc2	T	A	11: 105,227,482 (GRCm39)	Y572N	probably damaging	Het
Mterf3	T	C	13: 67,070,967 (GRCm39)	N172S	possibly damaging	Het
Or3a1c	T	A	11: 74,046,196 (GRCm39)	M72K	probably benign	Het
Or4b1	A	T	2: 89,979,068 (GRCm39)		probably benign	Het
Or4c115	T	A	2: 88,928,064 (GRCm39)	D69V	probably damaging	Het
Or5ak4	A	T	2: 85,161,687 (GRCm39)	L185H	probably damaging	Het
Or6c6c	T	C	10: 129,541,487 (GRCm39)	S247P	probably damaging	Het
Padi4	T	G	4: 140,484,881 (GRCm39)	T327P	possibly damaging	Het
Pga5	T	A	19: 10,651,201 (GRCm39)	I151F	probably benign	Het
Pkdrej	G	T	15: 85,702,275 (GRCm39)	D1220E	probably benign	Het
Prokr2	T	A	2: 132,215,684 (GRCm39)	Q259L	probably damaging	Het
Ptbp2	C	A	3: 119,546,754 (GRCm39)	D43Y	probably damaging	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Ralgps2	C	T	1: 156,660,500 (GRCm39)	R237Q	possibly damaging	Het
Rap1gds1	C	A	3: 138,671,624 (GRCm39)		probably null	Het
Saa2	T	A	7: 46,401,716 (GRCm39)	M1K	probably null	Het
Samd9l	T	C	6: 3,375,426 (GRCm39)	I612V	probably damaging	Het
Scn10a	C	T	9: 119,442,692 (GRCm39)	V1518I	probably benign	Het
Serpina6	T	C	12: 103,618,012 (GRCm39)	D267G	probably damaging	Het
Sh2d4b	A	G	14: 40,564,329 (GRCm39)		probably null	Het
Sh3bp2	T	C	5: 34,718,034 (GRCm39)	V505A	probably benign	Het
Smad2	A	G	18: 76,395,657 (GRCm39)	E32G	possibly damaging	Het
Smn1	T	G	13: 100,263,118 (GRCm39)	D32E	probably damaging	Het
Spata31d1c	T	C	13: 65,182,883 (GRCm39)	S142P	possibly damaging	Het
Stk39	A	T	2: 68,221,293 (GRCm39)	I210N	probably damaging	Het
Tcte2	A	G	17: 13,937,899 (GRCm39)		probably benign	Het
Tctn3	C	A	19: 40,597,361 (GRCm39)	E230*	probably null	Het
Tenm2	A	T	11: 35,937,896 (GRCm39)	H1592Q	probably damaging	Het
Tescl	A	G	7: 24,032,668 (GRCm39)	V219A	probably damaging	Het
Tgm6	C	T	2: 129,993,660 (GRCm39)	S633L	probably benign	Het
Tmcc1	C	T	6: 116,110,924 (GRCm39)	S123N	probably damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Ulk2	G	A	11: 61,670,581 (GRCm39)	R992C	probably damaging	Het
Vwa5b1	G	A	4: 138,332,184 (GRCm39)	H278Y	probably damaging	Het
Wwp2	C	T	8: 108,275,121 (GRCm39)	R373W	probably damaging	Het
Zfp24	A	T	18: 24,150,399 (GRCm39)	D170E	possibly damaging	Het
Zfp808	T	C	13: 62,319,311 (GRCm39)	I180T	possibly damaging	Het
Zfp820	T	A	17: 22,037,737 (GRCm39)	Q530H	probably benign	Het
Zfp975	A	T	7: 42,311,507 (GRCm39)	Y369N	probably benign	Het

Other mutations in Mki67

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00225:Mki67	APN	7	135,291,849 (GRCm39)	missense	probably benign	0.32
IGL00264:Mki67	APN	7	135,309,549 (GRCm39)	nonsense	probably null
IGL00328:Mki67	APN	7	135,298,424 (GRCm39)	missense	probably benign	0.03
IGL00570:Mki67	APN	7	135,309,830 (GRCm39)	missense	possibly damaging	0.88
IGL00584:Mki67	APN	7	135,297,424 (GRCm39)	missense	probably damaging	1.00
IGL00756:Mki67	APN	7	135,300,460 (GRCm39)	missense	possibly damaging	0.76
IGL01063:Mki67	APN	7	135,296,651 (GRCm39)	missense	possibly damaging	0.93
IGL01112:Mki67	APN	7	135,315,745 (GRCm39)	missense	probably damaging	1.00
IGL01360:Mki67	APN	7	135,307,505 (GRCm39)	missense	probably damaging	1.00
IGL01457:Mki67	APN	7	135,301,275 (GRCm39)	missense	probably benign	0.00
IGL01686:Mki67	APN	7	135,309,542 (GRCm39)	missense	probably benign	0.00
IGL01731:Mki67	APN	7	135,298,278 (GRCm39)	missense	probably benign	0.03
IGL01775:Mki67	APN	7	135,300,005 (GRCm39)	missense	possibly damaging	0.71
IGL01806:Mki67	APN	7	135,300,686 (GRCm39)	missense	probably damaging	0.98
IGL01860:Mki67	APN	7	135,300,686 (GRCm39)	missense	probably damaging	0.98
IGL01938:Mki67	APN	7	135,296,059 (GRCm39)	missense	probably benign	0.04
IGL02249:Mki67	APN	7	135,302,251 (GRCm39)	missense	possibly damaging	0.47
IGL02260:Mki67	APN	7	135,303,697 (GRCm39)	missense	probably benign	0.00
IGL02270:Mki67	APN	7	135,300,361 (GRCm39)	missense	probably damaging	1.00
IGL02406:Mki67	APN	7	135,300,522 (GRCm39)	missense	probably benign	0.00
IGL02499:Mki67	APN	7	135,296,056 (GRCm39)	missense	possibly damaging	0.94
IGL02655:Mki67	APN	7	135,315,748 (GRCm39)	missense	probably damaging	0.98
IGL02700:Mki67	APN	7	135,309,931 (GRCm39)	missense	probably benign	0.02
IGL03370:Mki67	APN	7	135,297,219 (GRCm39)	missense	probably benign	0.00
Advisement	UTSW	7	135,299,923 (GRCm39)	missense	probably damaging	1.00
chocotoff	UTSW	7	135,300,628 (GRCm39)	missense	possibly damaging	0.92
Godiva	UTSW	7	135,303,691 (GRCm39)	missense	probably benign	0.10
sees	UTSW	7	135,302,644 (GRCm39)	missense	possibly damaging	0.68
Whitman	UTSW	7	135,315,594 (GRCm39)	missense	probably damaging	1.00
BB003:Mki67	UTSW	7	135,298,869 (GRCm39)	missense	possibly damaging	0.91
BB013:Mki67	UTSW	7	135,298,869 (GRCm39)	missense	possibly damaging	0.91
PIT4468001:Mki67	UTSW	7	135,300,876 (GRCm39)	missense	probably benign	0.00
R0001:Mki67	UTSW	7	135,302,748 (GRCm39)	missense	probably damaging	0.99
R0001:Mki67	UTSW	7	135,300,901 (GRCm39)	missense	probably damaging	1.00
R0043:Mki67	UTSW	7	135,302,310 (GRCm39)	missense	probably benign	0.16
R0043:Mki67	UTSW	7	135,302,310 (GRCm39)	missense	probably benign	0.16
R0102:Mki67	UTSW	7	135,315,532 (GRCm39)	missense	probably benign	0.16
R0130:Mki67	UTSW	7	135,298,188 (GRCm39)	missense	probably damaging	1.00
R0149:Mki67	UTSW	7	135,300,153 (GRCm39)	missense	probably benign	0.00
R0356:Mki67	UTSW	7	135,306,135 (GRCm39)	missense	probably benign	0.34
R0482:Mki67	UTSW	7	135,301,158 (GRCm39)	missense	possibly damaging	0.60
R0508:Mki67	UTSW	7	135,302,075 (GRCm39)	missense	probably benign
R0532:Mki67	UTSW	7	135,299,893 (GRCm39)	nonsense	probably null
R0548:Mki67	UTSW	7	135,298,637 (GRCm39)	missense	possibly damaging	0.82
R0548:Mki67	UTSW	7	135,296,985 (GRCm39)	missense	probably damaging	1.00
R0557:Mki67	UTSW	7	135,300,990 (GRCm39)	missense	possibly damaging	0.48
R0627:Mki67	UTSW	7	135,309,987 (GRCm39)	missense	probably benign	0.31
R0631:Mki67	UTSW	7	135,306,117 (GRCm39)	missense	probably damaging	0.98
R0848:Mki67	UTSW	7	135,302,772 (GRCm39)	missense	probably benign	0.21
R1075:Mki67	UTSW	7	135,299,040 (GRCm39)	missense	probably benign	0.03
R1105:Mki67	UTSW	7	135,302,779 (GRCm39)	missense	probably benign	0.09
R1272:Mki67	UTSW	7	135,302,143 (GRCm39)	nonsense	probably null
R1331:Mki67	UTSW	7	135,300,005 (GRCm39)	missense	possibly damaging	0.71
R1486:Mki67	UTSW	7	135,301,449 (GRCm39)	missense	probably benign	0.00
R1510:Mki67	UTSW	7	135,297,900 (GRCm39)	missense	probably benign	0.26
R1586:Mki67	UTSW	7	135,315,701 (GRCm39)	nonsense	probably null
R1599:Mki67	UTSW	7	135,301,663 (GRCm39)	missense	probably benign	0.34
R1623:Mki67	UTSW	7	135,310,547 (GRCm39)	splice site	probably null
R1706:Mki67	UTSW	7	135,302,295 (GRCm39)	missense	probably benign	0.37
R1718:Mki67	UTSW	7	135,297,223 (GRCm39)	missense	probably damaging	1.00
R1785:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R1816:Mki67	UTSW	7	135,309,116 (GRCm39)	missense	possibly damaging	0.68
R1862:Mki67	UTSW	7	135,301,090 (GRCm39)	missense	probably benign	0.09
R1929:Mki67	UTSW	7	135,299,794 (GRCm39)	missense	possibly damaging	0.46
R1957:Mki67	UTSW	7	135,300,128 (GRCm39)	missense	probably benign	0.01
R1971:Mki67	UTSW	7	135,315,688 (GRCm39)	critical splice donor site	probably null
R1998:Mki67	UTSW	7	135,307,499 (GRCm39)	missense	probably benign	0.00
R2004:Mki67	UTSW	7	135,300,238 (GRCm39)	nonsense	probably null
R2005:Mki67	UTSW	7	135,300,238 (GRCm39)	nonsense	probably null
R2006:Mki67	UTSW	7	135,300,238 (GRCm39)	nonsense	probably null
R2109:Mki67	UTSW	7	135,299,592 (GRCm39)	missense	probably damaging	1.00
R2130:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R2131:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R2133:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R2140:Mki67	UTSW	7	135,297,321 (GRCm39)	missense	possibly damaging	0.94
R2141:Mki67	UTSW	7	135,297,321 (GRCm39)	missense	possibly damaging	0.94
R2142:Mki67	UTSW	7	135,297,321 (GRCm39)	missense	possibly damaging	0.94
R2284:Mki67	UTSW	7	135,301,674 (GRCm39)	missense	probably damaging	0.99
R2869:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2869:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2871:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2871:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2913:Mki67	UTSW	7	135,302,415 (GRCm39)	missense	possibly damaging	0.71
R3404:Mki67	UTSW	7	135,309,204 (GRCm39)	missense	probably benign	0.01
R3405:Mki67	UTSW	7	135,309,204 (GRCm39)	missense	probably benign	0.01
R3406:Mki67	UTSW	7	135,309,204 (GRCm39)	missense	probably benign	0.01
R3777:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3778:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3787:Mki67	UTSW	7	135,302,012 (GRCm39)	missense	possibly damaging	0.93
R3847:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3848:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3853:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3971:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3972:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R4258:Mki67	UTSW	7	135,297,017 (GRCm39)	missense	possibly damaging	0.86
R4343:Mki67	UTSW	7	135,296,847 (GRCm39)	missense	probably benign	0.10
R4488:Mki67	UTSW	7	135,299,400 (GRCm39)	missense	probably benign	0.01
R4528:Mki67	UTSW	7	135,297,088 (GRCm39)	missense	probably damaging	1.00
R4713:Mki67	UTSW	7	135,297,198 (GRCm39)	missense	probably benign	0.35
R4867:Mki67	UTSW	7	135,301,585 (GRCm39)	missense	probably damaging	0.97
R4874:Mki67	UTSW	7	135,310,500 (GRCm39)	missense	probably damaging	0.97
R4897:Mki67	UTSW	7	135,298,474 (GRCm39)	missense	probably damaging	1.00
R5045:Mki67	UTSW	7	135,309,633 (GRCm39)	missense	possibly damaging	0.84
R5306:Mki67	UTSW	7	135,315,730 (GRCm39)	missense	probably damaging	1.00
R5309:Mki67	UTSW	7	135,302,559 (GRCm39)	missense	probably damaging	1.00
R5312:Mki67	UTSW	7	135,302,559 (GRCm39)	missense	probably damaging	1.00
R5379:Mki67	UTSW	7	135,299,190 (GRCm39)	missense	possibly damaging	0.95
R5506:Mki67	UTSW	7	135,301,710 (GRCm39)	missense	possibly damaging	0.60
R5513:Mki67	UTSW	7	135,309,479 (GRCm39)	missense	probably damaging	0.98
R5742:Mki67	UTSW	7	135,306,102 (GRCm39)	missense	probably benign	0.20
R5806:Mki67	UTSW	7	135,306,334 (GRCm39)	missense	probably damaging	1.00
R6008:Mki67	UTSW	7	135,299,158 (GRCm39)	missense	probably damaging	1.00
R6037:Mki67	UTSW	7	135,298,532 (GRCm39)	missense	possibly damaging	0.69
R6037:Mki67	UTSW	7	135,298,532 (GRCm39)	missense	possibly damaging	0.69
R6221:Mki67	UTSW	7	135,299,643 (GRCm39)	missense	probably benign	0.18
R6294:Mki67	UTSW	7	135,306,319 (GRCm39)	missense	probably benign	0.09
R6377:Mki67	UTSW	7	135,298,050 (GRCm39)	missense	possibly damaging	0.67
R6456:Mki67	UTSW	7	135,301,204 (GRCm39)	missense	possibly damaging	0.59
R6608:Mki67	UTSW	7	135,300,090 (GRCm39)	missense	probably benign	0.01
R6609:Mki67	UTSW	7	135,301,558 (GRCm39)	missense	possibly damaging	0.94
R6648:Mki67	UTSW	7	135,299,169 (GRCm39)	missense	probably damaging	1.00
R6901:Mki67	UTSW	7	135,310,489 (GRCm39)	splice site	probably null
R6978:Mki67	UTSW	7	135,303,691 (GRCm39)	missense	probably benign	0.10
R6985:Mki67	UTSW	7	135,315,594 (GRCm39)	missense	probably damaging	1.00
R7076:Mki67	UTSW	7	135,307,358 (GRCm39)	missense	probably damaging	0.98
R7217:Mki67	UTSW	7	135,305,911 (GRCm39)	missense	probably damaging	1.00
R7239:Mki67	UTSW	7	135,301,905 (GRCm39)	missense	possibly damaging	0.91
R7250:Mki67	UTSW	7	135,301,053 (GRCm39)	missense	possibly damaging	0.90
R7313:Mki67	UTSW	7	135,296,400 (GRCm39)	missense	probably benign	0.29
R7336:Mki67	UTSW	7	135,315,568 (GRCm39)	missense	probably benign	0.03
R7422:Mki67	UTSW	7	135,300,099 (GRCm39)	missense	probably damaging	1.00
R7451:Mki67	UTSW	7	135,301,080 (GRCm39)	missense	probably benign	0.01
R7502:Mki67	UTSW	7	135,302,512 (GRCm39)	missense	possibly damaging	0.53
R7513:Mki67	UTSW	7	135,294,952 (GRCm39)	missense	probably benign
R7578:Mki67	UTSW	7	135,302,644 (GRCm39)	missense	possibly damaging	0.68
R7619:Mki67	UTSW	7	135,301,106 (GRCm39)	missense	probably benign	0.01
R7646:Mki67	UTSW	7	135,298,498 (GRCm39)	missense	possibly damaging	0.63
R7659:Mki67	UTSW	7	135,299,155 (GRCm39)	missense	probably damaging	1.00
R7691:Mki67	UTSW	7	135,303,721 (GRCm39)	missense	not run
R7780:Mki67	UTSW	7	135,315,697 (GRCm39)	missense	probably benign	0.02
R7796:Mki67	UTSW	7	135,299,923 (GRCm39)	missense	probably damaging	1.00
R7904:Mki67	UTSW	7	135,294,816 (GRCm39)	missense	possibly damaging	0.90
R7911:Mki67	UTSW	7	135,306,333 (GRCm39)	missense	probably damaging	1.00
R7921:Mki67	UTSW	7	135,296,933 (GRCm39)	missense	probably benign	0.01
R7926:Mki67	UTSW	7	135,298,869 (GRCm39)	missense	possibly damaging	0.91
R7950:Mki67	UTSW	7	135,301,453 (GRCm39)	nonsense	probably null
R8130:Mki67	UTSW	7	135,299,293 (GRCm39)	missense	probably damaging	1.00
R8145:Mki67	UTSW	7	135,296,065 (GRCm39)	missense	probably benign	0.07
R8196:Mki67	UTSW	7	135,297,237 (GRCm39)	missense	probably damaging	1.00
R8220:Mki67	UTSW	7	135,299,850 (GRCm39)	missense	probably benign	0.03
R8299:Mki67	UTSW	7	135,306,349 (GRCm39)	missense	probably damaging	1.00
R8334:Mki67	UTSW	7	135,298,245 (GRCm39)	missense	probably damaging	0.98
R8350:Mki67	UTSW	7	135,300,200 (GRCm39)	missense	possibly damaging	0.82
R8358:Mki67	UTSW	7	135,301,855 (GRCm39)	missense	possibly damaging	0.46
R8529:Mki67	UTSW	7	135,315,688 (GRCm39)	critical splice donor site	probably null
R8698:Mki67	UTSW	7	135,296,937 (GRCm39)	missense	possibly damaging	0.87
R8700:Mki67	UTSW	7	135,307,436 (GRCm39)	missense
R8737:Mki67	UTSW	7	135,315,504 (GRCm39)	missense	probably damaging	1.00
R8914:Mki67	UTSW	7	135,299,595 (GRCm39)	missense
R8930:Mki67	UTSW	7	135,300,628 (GRCm39)	missense	possibly damaging	0.92
R8932:Mki67	UTSW	7	135,300,628 (GRCm39)	missense	possibly damaging	0.92
R8972:Mki67	UTSW	7	135,297,364 (GRCm39)	missense	possibly damaging	0.54
R8973:Mki67	UTSW	7	135,297,364 (GRCm39)	missense	possibly damaging	0.54
R8975:Mki67	UTSW	7	135,300,129 (GRCm39)	missense	probably benign	0.01
R8975:Mki67	UTSW	7	135,297,364 (GRCm39)	missense	possibly damaging	0.54
R9071:Mki67	UTSW	7	135,301,205 (GRCm39)	missense	probably benign	0.00
R9241:Mki67	UTSW	7	135,297,653 (GRCm39)	missense	possibly damaging	0.93
R9387:Mki67	UTSW	7	135,302,378 (GRCm39)	missense	probably damaging	0.99
R9524:Mki67	UTSW	7	135,305,913 (GRCm39)	missense	probably damaging	1.00
R9565:Mki67	UTSW	7	135,309,233 (GRCm39)	frame shift	probably null
R9782:Mki67	UTSW	7	135,306,066 (GRCm39)	critical splice donor site	probably null
X0020:Mki67	UTSW	7	135,315,730 (GRCm39)	missense	probably damaging	0.96
X0065:Mki67	UTSW	7	135,315,573 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- GCTTTGGAAGCTGTCAACTTGCTC -3'
(R):5'- TTTAACTGGCAGGAGGAACCAACC -3'

Sequencing Primer
(F):5'- ACTTGCTCTGGTTGTAGAGAATTAC -3'
(R):5'- GAACTCACCAGTTTCCAAGAGG -3'

Posted On

2014-04-13