Mutagenetix > Incidental Mutation

Incidental Mutation 'R1575:Tg'

171127

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

039613-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R1575 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

66542606-66722570 bp(+) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to C at 66601534 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000126454 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916] [ENSMUST00000065916] [ENSMUST00000163495] [ENSMUST00000163495] [ENSMUST00000171045] [ENSMUST00000171045] [ENSMUST00000171045] [ENSMUST00000171045]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000065916

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000065916

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163495

SMART Domains

Protein: ENSMUSP00000129868
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
TY	14	63	1.21e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000163495

SMART Domains

Protein: ENSMUSP00000129868
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
TY	14	63	1.21e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000166906

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167344

Predicted Effect

probably null
Transcript: ENSMUST00000171045

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000171045

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000171045

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000171045

SMART Domains

Protein: ENSMUSP00000126454
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
internal_repeat_1	93	331	1.53e-6	PROSPERO
Pfam:COesterase	562	1098	2.1e-137	PFAM

Meta Mutation Damage Score

0.9348

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.0%
20x: 91.5%

Validation Efficiency

100% (72/72)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl2	T	A	3: 148,558,398 (GRCm39)	T437S	probably benign	Het
Akna	A	G	4: 63,297,570 (GRCm39)	F828S	probably benign	Het
Alg9	G	T	9: 50,686,802 (GRCm39)	A40S	possibly damaging	Het
Alox8	T	A	11: 69,076,067 (GRCm39)	H628L	possibly damaging	Het
Aox3	T	C	1: 58,191,713 (GRCm39)	W422R	probably benign	Het
Atp13a4	T	C	16: 29,228,528 (GRCm39)	D984G	probably benign	Het
Bcam	T	C	7: 19,494,307 (GRCm39)	E363G	possibly damaging	Het
Cadps2	C	A	6: 23,429,217 (GRCm39)	V519F	probably damaging	Het
Calca	A	G	7: 114,234,396 (GRCm39)	Y18H	probably damaging	Het
Cd70	A	G	17: 57,453,364 (GRCm39)	I100T	probably damaging	Het
Cdk4	T	A	10: 126,900,520 (GRCm39)	H95Q	probably damaging	Het
Chchd1	T	A	14: 20,753,410 (GRCm39)	N11K	probably damaging	Het
Cma2	T	A	14: 56,210,272 (GRCm39)	N52K	probably damaging	Het
Cyp3a16	A	G	5: 145,373,267 (GRCm39)	V500A	probably benign	Het
Dicer1	A	G	12: 104,688,228 (GRCm39)		probably null	Het
Dnajc13	A	G	9: 104,034,037 (GRCm39)	S2206P	probably benign	Het
Dtl	T	C	1: 191,293,658 (GRCm39)		probably null	Het
Fam186b	T	C	15: 99,184,852 (GRCm39)	T24A	probably benign	Het
Fbxw21	A	G	9: 108,990,984 (GRCm39)	V25A	probably benign	Het
Gins1	T	C	2: 150,754,758 (GRCm39)	S45P	probably benign	Het
Gtpbp2	T	A	17: 46,476,869 (GRCm39)	V349D	probably damaging	Het
Hyal5	G	T	6: 24,876,792 (GRCm39)	D222Y	probably damaging	Het
Itgal	A	G	7: 126,900,060 (GRCm39)		probably null	Het
Klk14	A	G	7: 43,343,377 (GRCm39)		probably null	Het
Lama1	T	A	17: 68,117,404 (GRCm39)	L2518Q	possibly damaging	Het
Lrrc2	G	A	9: 110,808,555 (GRCm39)	G264D	probably benign	Het
Ltbp4	A	G	7: 27,022,245 (GRCm39)	S893P	probably damaging	Het
Mast4	G	A	13: 102,875,771 (GRCm39)	P1107L	probably damaging	Het
Mbd1	T	A	18: 74,408,490 (GRCm39)		probably null	Het
Naip2	A	T	13: 100,291,529 (GRCm39)	D1136E	probably benign	Het
Naip2	G	A	13: 100,291,537 (GRCm39)		probably benign	Het
Ncan	G	A	8: 70,562,848 (GRCm39)	T470I	probably benign	Het
Npy1r	A	T	8: 67,156,813 (GRCm39)	I78F	probably damaging	Het
Or10q1b	A	T	19: 13,682,889 (GRCm39)	M233L	probably benign	Het
Or8b52	G	A	9: 38,576,573 (GRCm39)	T189M	probably damaging	Het
Palb2	A	T	7: 121,710,061 (GRCm39)		probably null	Het
Pax3	T	C	1: 78,080,121 (GRCm39)	T422A	probably benign	Het
Pebp1	A	T	5: 117,424,229 (GRCm39)	D72E	possibly damaging	Het
Pnliprp1	A	T	19: 58,728,901 (GRCm39)	T363S	probably benign	Het
Rbm44	G	A	1: 91,084,565 (GRCm39)		probably null	Het
Rbm47	T	A	5: 66,182,358 (GRCm39)	Y425F	probably benign	Het
Robo3	G	T	9: 37,340,957 (GRCm39)	A83E	probably damaging	Het
Rrm1	A	G	7: 102,105,721 (GRCm39)	Y279C	probably damaging	Het
Rslcan18	A	T	13: 67,256,121 (GRCm39)		probably benign	Het
Scara5	C	A	14: 65,968,314 (GRCm39)	Q196K	probably benign	Het
Setd1b	C	T	5: 123,301,210 (GRCm39)		probably benign	Het
Siah1a	A	G	8: 87,451,869 (GRCm39)	F205S	probably damaging	Het
Smr2	AT	ATT	5: 88,256,683 (GRCm39)		probably null	Het
Ssu72	A	G	4: 155,815,814 (GRCm39)	D86G	probably benign	Het
St7	G	T	6: 17,886,110 (GRCm39)	K357N	probably damaging	Het
Sv2b	G	A	7: 74,797,425 (GRCm39)	T323I	probably damaging	Het
Syt1	T	C	10: 108,340,361 (GRCm39)	N319S	probably benign	Het
Tanc1	T	A	2: 59,621,995 (GRCm39)	F371L	probably damaging	Het
Tcf20	C	A	15: 82,739,693 (GRCm39)	G586V	probably benign	Het
Tyk2	A	T	9: 21,026,758 (GRCm39)	N620K	probably benign	Het
Ube2j1	T	A	4: 33,045,116 (GRCm39)	S130T	probably benign	Het
Ubr2	G	T	17: 47,243,418 (GRCm39)	P1696H	probably damaging	Het
Ubr5	A	T	15: 38,041,085 (GRCm39)	D266E	probably damaging	Het
Vipr2	A	G	12: 116,107,892 (GRCm39)	T426A	probably benign	Het
Vmn2r104	A	T	17: 20,262,477 (GRCm39)	W218R	probably damaging	Het
Vmn2r83	T	A	10: 79,314,956 (GRCm39)	N401K	probably damaging	Het
Vwf	A	G	6: 125,632,214 (GRCm39)	E82G	unknown	Het
Vwf	T	A	6: 125,640,534 (GRCm39)	Y2323*	probably null	Het
Wdr76	T	G	2: 121,359,402 (GRCm39)	V329G	probably damaging	Het
Zan	A	G	5: 137,460,214 (GRCm39)	C1226R	unknown	Het
Zbtb16	G	T	9: 48,743,572 (GRCm39)	Q247K	probably damaging	Het
Zfp541	T	C	7: 15,812,640 (GRCm39)	V431A	possibly damaging	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,719,015 (GRCm39)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,699,139 (GRCm39)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,565,273 (GRCm39)	missense	probably benign
IGL00428:Tg	APN	15	66,645,273 (GRCm39)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,568,338 (GRCm39)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,555,662 (GRCm39)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,560,650 (GRCm39)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,545,922 (GRCm39)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,636,302 (GRCm39)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,553,731 (GRCm39)	missense	probably benign
IGL01288:Tg	APN	15	66,608,125 (GRCm39)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,567,941 (GRCm39)	splice site	probably benign
IGL01634:Tg	APN	15	66,601,415 (GRCm39)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,549,936 (GRCm39)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,543,200 (GRCm39)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,631,335 (GRCm39)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,564,223 (GRCm39)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,577,179 (GRCm39)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,589,082 (GRCm39)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,577,197 (GRCm39)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,629,792 (GRCm39)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,636,140 (GRCm39)	missense	probably benign
IGL02434:Tg	APN	15	66,636,191 (GRCm39)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,613,443 (GRCm39)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,577,123 (GRCm39)	missense	probably benign
IGL02549:Tg	APN	15	66,711,210 (GRCm39)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,620,575 (GRCm39)	splice site	probably benign
IGL02756:Tg	APN	15	66,606,435 (GRCm39)	missense	probably benign
IGL02800:Tg	APN	15	66,629,735 (GRCm39)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,554,243 (GRCm39)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,543,254 (GRCm39)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,586,955 (GRCm39)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,711,152 (GRCm39)	nonsense	probably null
IGL03242:Tg	APN	15	66,555,647 (GRCm39)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
foster	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
hognose	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
ito	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
ito2	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
ito3	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
ito4	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
Papua	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
pluribus	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
samarai	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
sariba	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
ticker	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
Vampire	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,612,567 (GRCm39)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,612,630 (GRCm39)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,570,295 (GRCm39)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,636,291 (GRCm39)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,700,382 (GRCm39)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,554,253 (GRCm39)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,601,446 (GRCm39)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,601,475 (GRCm39)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,609,370 (GRCm39)	missense	probably benign
R0673:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,711,253 (GRCm39)	splice site	probably benign
R0704:Tg	UTSW	15	66,629,729 (GRCm39)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,550,638 (GRCm39)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,596,993 (GRCm39)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,579,859 (GRCm39)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,544,258 (GRCm39)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,570,408 (GRCm39)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,555,911 (GRCm39)	missense	probably benign
R1103:Tg	UTSW	15	66,591,504 (GRCm39)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,700,397 (GRCm39)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,568,338 (GRCm39)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,722,351 (GRCm39)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,577,081 (GRCm39)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,565,279 (GRCm39)	missense	possibly damaging	0.52
R1638:Tg	UTSW	15	66,568,015 (GRCm39)	nonsense	probably null
R1655:Tg	UTSW	15	66,700,417 (GRCm39)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,564,236 (GRCm39)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,609,397 (GRCm39)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,543,158 (GRCm39)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,554,691 (GRCm39)	missense	probably benign
R2063:Tg	UTSW	15	66,700,402 (GRCm39)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,721,456 (GRCm39)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,601,443 (GRCm39)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,553,788 (GRCm39)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,553,782 (GRCm39)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,555,747 (GRCm39)	missense	probably benign
R2994:Tg	UTSW	15	66,553,802 (GRCm39)	missense	probably benign
R3904:Tg	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
R3946:Tg	UTSW	15	66,545,872 (GRCm39)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,556,039 (GRCm39)	missense	probably benign
R4245:Tg	UTSW	15	66,568,318 (GRCm39)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,637,996 (GRCm39)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,579,791 (GRCm39)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,607,120 (GRCm39)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,545,769 (GRCm39)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,565,168 (GRCm39)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,636,186 (GRCm39)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,568,435 (GRCm39)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,579,779 (GRCm39)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,553,662 (GRCm39)	splice site	probably null
R5049:Tg	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
R5073:Tg	UTSW	15	66,607,101 (GRCm39)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,550,629 (GRCm39)	nonsense	probably null
R5185:Tg	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,631,416 (GRCm39)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,550,704 (GRCm39)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,549,904 (GRCm39)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,611,017 (GRCm39)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,699,142 (GRCm39)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,557,149 (GRCm39)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,565,284 (GRCm39)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,709,906 (GRCm39)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,560,738 (GRCm39)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,555,842 (GRCm39)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,700,306 (GRCm39)	missense	probably damaging	1.00
R6146:Tg	UTSW	15	66,545,216 (GRCm39)	splice site	probably null
R6159:Tg	UTSW	15	66,607,096 (GRCm39)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,579,771 (GRCm39)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,543,160 (GRCm39)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,631,407 (GRCm39)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,607,108 (GRCm39)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,560,740 (GRCm39)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,568,095 (GRCm39)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,565,207 (GRCm39)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,545,392 (GRCm39)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,566,633 (GRCm39)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,597,121 (GRCm39)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,568,432 (GRCm39)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,568,437 (GRCm39)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,568,010 (GRCm39)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,566,617 (GRCm39)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,561,776 (GRCm39)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,636,267 (GRCm39)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,601,432 (GRCm39)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,721,453 (GRCm39)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,565,112 (GRCm39)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,555,663 (GRCm39)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,577,128 (GRCm39)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,555,923 (GRCm39)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,555,642 (GRCm39)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,645,247 (GRCm39)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
R8688:Tg	UTSW	15	66,566,802 (GRCm39)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,553,786 (GRCm39)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,555,891 (GRCm39)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,557,184 (GRCm39)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,645,332 (GRCm39)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,555,522 (GRCm39)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,570,310 (GRCm39)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,699,118 (GRCm39)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,557,246 (GRCm39)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,561,173 (GRCm39)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,718,923 (GRCm39)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,545,913 (GRCm39)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,607,109 (GRCm39)	nonsense	probably null
R9629:Tg	UTSW	15	66,555,587 (GRCm39)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,637,991 (GRCm39)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,561,839 (GRCm39)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,719,008 (GRCm39)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,554,303 (GRCm39)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,620,592 (GRCm39)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,721,396 (GRCm39)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,557,159 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- ACACTGGCCTATTCCCTTTGGCAG -3'
(R):5'- GCAGCTTCATGGGTGCCATTTC -3'

Sequencing Primer
(F):5'- TCAGGCCAATGCTACATGTG -3'
(R):5'- CTATACTATAAATGGAACCTGCGGAG -3'

Posted On

2014-04-13