Mutagenetix > Incidental Mutations

Incidental Mutation 'R1580:Vmn2r10'

171342

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r10

Ensembl Gene

ENSMUSG00000067010

Gene Name

vomeronasal 2, receptor 10

Synonyms

V2r16, VR16

MMRRC Submission

039617-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.178) question?

Stock #

R1580 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

108993412-109006471 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109006251 bp

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 62 (N62K)

Ref Sequence

ENSEMBL: ENSMUSP00000135274 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079163] [ENSMUST00000176594]

Predicted Effect

probably benign
Transcript: ENSMUST00000079163
AA Change: N62K

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000078162
Gene: ENSMUSG00000067010
AA Change: N62K

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	75	464	8.5e-29	PFAM
Pfam:NCD3G	506	560	3.8e-17	PFAM
Pfam:7tm_3	593	828	4e-55	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176594
AA Change: N62K

PolyPhen 2 Score 0.816 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000135274
Gene: ENSMUSG00000067010
AA Change: N62K

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC

Meta Mutation Damage Score

0.1228

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.4%
20x: 89.8%

Validation Efficiency

96% (43/45)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	C	T	1: 71,265,965	V2044I	possibly damaging	Het
Adgrv1	A	G	13: 81,466,160		probably null	Het
Arhgef38	T	C	3: 133,133,704	Q526R	probably benign	Het
Atp2c2	A	G	8: 119,752,987	N752D	probably benign	Het
Atp6v0a1	T	C	11: 101,029,204	I221T	probably damaging	Het
Atp8b5	T	C	4: 43,355,673	V551A	possibly damaging	Het
B3galnt1	A	G	3: 69,575,707	S74P	possibly damaging	Het
Bcl2l13	A	G	6: 120,865,714	I123V	probably benign	Het
Brms1l	A	T	12: 55,868,222	K305N	probably damaging	Het
Ccdc82	C	T	9: 13,252,760	R226C	probably damaging	Het
Chst9	T	G	18: 15,453,065	K147T	probably benign	Het
Clec16a	A	G	16: 10,595,898	R390G	probably damaging	Het
Clec5a	G	T	6: 40,585,219	H4N	probably benign	Het
Csmd1	T	A	8: 15,925,299	Q2970L	probably damaging	Het
Cyp2a4	T	C	7: 26,307,651	I61T	possibly damaging	Het
Cyp3a16	T	A	5: 145,442,074	K379I	possibly damaging	Het
Cyp3a16	T	C	5: 145,442,075	K379E	probably damaging	Het
Dok2	A	G	14: 70,776,957	D195G	probably benign	Het
Emilin1	A	G	5: 30,917,420	E335G	probably damaging	Het
Fam205c	T	C	4: 42,874,020		probably null	Het
Gm7361	G	T	5: 26,257,770	L3F	probably damaging	Het
Haus1	T	C	18: 77,766,920	D50G	probably damaging	Het
Igf1r	T	C	7: 68,207,869	V1099A	probably benign	Het
Kif15	T	C	9: 122,959,956	V71A	probably benign	Het
Klk10	C	T	7: 43,782,862	A73V	probably damaging	Het
Lins1	C	A	7: 66,714,491	D711E	probably benign	Het
Mbtps1	C	T	8: 119,538,900	V303I	possibly damaging	Het
Mest	G	A	6: 30,745,823		probably benign	Het
Nup214	C	T	2: 32,034,466	S1669F	probably damaging	Het
Olfr299	A	G	7: 86,465,450	E13G	probably benign	Het
Olfr800	G	T	10: 129,660,315	V170F	probably benign	Het
Rfwd3	C	T	8: 111,288,242	R326Q	probably damaging	Het
Rtf2	A	G	2: 172,445,365	D68G	probably damaging	Het
Sbspon	C	A	1: 15,892,468	C62F	probably damaging	Het
Spg7	T	A	8: 123,090,238		probably benign	Het
Trabd2b	T	C	4: 114,580,334	V236A	possibly damaging	Het
Vmn2r45	T	G	7: 8,471,747	S761R	possibly damaging	Het
Zfp580	C	T	7: 5,053,285	R215C	probably damaging	Het
Zfpm2	A	G	15: 41,103,209	D898G	possibly damaging	Het

Other mutations in Vmn2r10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00816:Vmn2r10	APN	5	109002585	missense	possibly damaging	0.75
IGL01618:Vmn2r10	APN	5	109002479	missense	probably damaging	1.00
IGL01624:Vmn2r10	APN	5	109006246	missense	possibly damaging	0.83
IGL01941:Vmn2r10	APN	5	108995954	missense	probably damaging	1.00
IGL01959:Vmn2r10	APN	5	108997705	missense	probably benign	0.00
IGL01973:Vmn2r10	APN	5	108995677	missense	probably damaging	1.00
IGL01985:Vmn2r10	APN	5	109006259	missense	probably benign	0.21
IGL02451:Vmn2r10	APN	5	108995922	nonsense	probably null
IGL02503:Vmn2r10	APN	5	109003475	missense	probably damaging	1.00
IGL03275:Vmn2r10	APN	5	109003377	missense	probably benign	0.00
R0035:Vmn2r10	UTSW	5	108997601	splice site	probably benign
R0395:Vmn2r10	UTSW	5	109001993	missense	probably damaging	1.00
R0454:Vmn2r10	UTSW	5	109003461	missense	probably benign	0.25
R0648:Vmn2r10	UTSW	5	108995916	missense	probably benign	0.00
R1251:Vmn2r10	UTSW	5	108996024	missense	probably benign	0.01
R1845:Vmn2r10	UTSW	5	109001995	nonsense	probably null
R1986:Vmn2r10	UTSW	5	109006254	nonsense	probably null
R2137:Vmn2r10	UTSW	5	109003544	missense	possibly damaging	0.87
R2495:Vmn2r10	UTSW	5	108996095	missense	probably damaging	1.00
R3932:Vmn2r10	UTSW	5	109002222	missense	possibly damaging	0.47
R3933:Vmn2r10	UTSW	5	109002222	missense	possibly damaging	0.47
R4899:Vmn2r10	UTSW	5	109003458	missense	probably damaging	1.00
R4992:Vmn2r10	UTSW	5	108997726	missense	possibly damaging	0.89
R5124:Vmn2r10	UTSW	5	109006420	missense	probably benign	0.01
R5145:Vmn2r10	UTSW	5	108995895	missense	possibly damaging	0.64
R5156:Vmn2r10	UTSW	5	108995600	missense	probably benign	0.11
R5265:Vmn2r10	UTSW	5	108995720	missense	probably damaging	1.00
R5311:Vmn2r10	UTSW	5	109006255	missense	probably damaging	1.00
R5321:Vmn2r10	UTSW	5	108995639	missense	probably damaging	1.00
R5666:Vmn2r10	UTSW	5	108999044	nonsense	probably null
R5670:Vmn2r10	UTSW	5	108999044	nonsense	probably null
R5872:Vmn2r10	UTSW	5	109003511	missense	possibly damaging	0.84
R6004:Vmn2r10	UTSW	5	108999078	missense	probably benign	0.30
R6108:Vmn2r10	UTSW	5	108995801	missense	probably damaging	1.00
R6332:Vmn2r10	UTSW	5	109003462	missense	probably damaging	1.00
R6599:Vmn2r10	UTSW	5	108996078	missense	probably benign	0.09
R6651:Vmn2r10	UTSW	5	108995622	missense	probably null	0.22
R6891:Vmn2r10	UTSW	5	109001979	missense	probably damaging	1.00
Z1088:Vmn2r10	UTSW	5	108996113	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAGGTGGTGATTGTGAATTTAAGGCA -3'
(R):5'- GTGGATCTTCAGAGAGCAGTAGCCATA -3'

Sequencing Primer
(F):5'- GGCAGATGATGAGACATCTTTC -3'
(R):5'- CCTTAGCAGATATGAAAAACCTGTG -3'

Posted On

2014-04-13