Mutagenetix > Incidental Mutation

Incidental Mutation 'R1646:Or7a35'

173890

Institutional Source

Beutler Lab

Gene Symbol

Or7a35

Ensembl Gene

ENSMUSG00000063216

Gene Name

olfactory receptor family 7 subfamily A member 35

Synonyms

Olfr1351, GA_x6K02T2QGN0-2794907-2793948, MOR139-4

MMRRC Submission

039682-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.139) question?

Stock #

R1646 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

78853158-78854117 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 78853340 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 61 (Y61*)

Ref Sequence

ENSEMBL: ENSMUSP00000149241 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080730] [ENSMUST00000203906] [ENSMUST00000216030]

AlphaFold

Q8VGU6

Predicted Effect

probably null
Transcript: ENSMUST00000080730
AA Change: Y61*

SMART Domains

Protein: ENSMUSP00000079555
Gene: ENSMUSG00000063216
AA Change: Y61*

Domain	Start	End	E-Value	Type
low complexity region	9	19	N/A	INTRINSIC
Pfam:7tm_4	32	308	2e-49	PFAM
Pfam:7tm_1	42	291	1.9e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203906
AA Change: Y61*

SMART Domains

Protein: ENSMUSP00000144814
Gene: ENSMUSG00000060205
AA Change: Y61*

Domain	Start	End	E-Value	Type
low complexity region	9	19	N/A	INTRINSIC
Pfam:7tm_4	32	308	2e-49	PFAM
Pfam:7tm_1	42	291	1.9e-23	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216030
AA Change: Y61*

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219714

Meta Mutation Damage Score

0.9718

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.1%
20x: 91.8%

Validation Efficiency

96% (69/72)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akna	T	G	4: 63,302,129 (GRCm39)	I581L	probably benign	Het
Cacnb4	A	G	2: 52,364,912 (GRCm39)	I117T	possibly damaging	Het
Capn1	A	T	19: 6,047,760 (GRCm39)	F434L	probably benign	Het
Cbs	T	C	17: 31,832,169 (GRCm39)	T547A	probably benign	Het
Col6a5	A	T	9: 105,739,948 (GRCm39)	L2557*	probably null	Het
Dach1	A	G	14: 98,406,550 (GRCm39)	S66P	unknown	Het
Ddx31	T	C	2: 28,782,532 (GRCm39)	V625A	probably benign	Het
Dmxl1	T	G	18: 50,095,328 (GRCm39)	V2969G	probably damaging	Het
Eapp	T	A	12: 54,732,745 (GRCm39)	K122*	probably null	Het
Elapor1	A	T	3: 108,370,306 (GRCm39)	S751T	probably damaging	Het
Epb41l5	G	T	1: 119,477,752 (GRCm39)		probably benign	Het
Fat1	T	C	8: 45,471,079 (GRCm39)	S1628P	probably damaging	Het
Fgfr2	T	A	7: 129,844,374 (GRCm39)	E37V	probably damaging	Het
Fgfr4	A	T	13: 55,313,777 (GRCm39)	N529Y	probably damaging	Het
Fsip2	A	T	2: 82,808,861 (GRCm39)	T1727S	probably benign	Het
Gak	A	T	5: 108,750,720 (GRCm39)	S397T	probably damaging	Het
Gm6040	T	A	8: 21,407,113 (GRCm39)	I36F	possibly damaging	Het
Grhl1	A	G	12: 24,661,860 (GRCm39)	D513G	possibly damaging	Het
Gstt1	T	A	10: 75,619,940 (GRCm39)	D219V	possibly damaging	Het
Hcfc2	T	G	10: 82,536,861 (GRCm39)	V91G	probably damaging	Het
Hells	A	T	19: 38,956,227 (GRCm39)	I808L	probably benign	Het
Icmt	T	A	4: 152,384,172 (GRCm39)	V110E	possibly damaging	Het
Ilrun	A	G	17: 28,012,934 (GRCm39)	S88P	probably damaging	Het
Iqca1	C	T	1: 90,067,760 (GRCm39)	V164M	probably damaging	Het
Klri1	G	A	6: 129,680,299 (GRCm39)	P119S	probably benign	Het
Krt71	C	A	15: 101,647,199 (GRCm39)		probably null	Het
Lpin1	A	G	12: 16,623,659 (GRCm39)		probably null	Het
Macir	A	T	1: 97,573,531 (GRCm39)	I178N	probably damaging	Het
Metap2	T	C	10: 93,706,059 (GRCm39)	H241R	probably damaging	Het
Myh15	A	G	16: 49,015,931 (GRCm39)	Y1869C	probably damaging	Het
Myo1h	G	A	5: 114,455,693 (GRCm39)	G59E	possibly damaging	Het
Ncam2	T	G	16: 81,262,594 (GRCm39)		probably benign	Het
Npat	T	C	9: 53,466,434 (GRCm39)	V241A	probably benign	Het
Npbwr1	C	A	1: 5,987,473 (GRCm39)	V14L	probably benign	Het
Nup37	T	A	10: 88,014,096 (GRCm39)	V323E	possibly damaging	Het
Or11g27	A	T	14: 50,771,040 (GRCm39)	Q57L	probably benign	Het
Or5m9b	A	G	2: 85,905,960 (GRCm39)	N292S	probably damaging	Het
Or5p59	C	T	7: 107,702,798 (GRCm39)	T94I	probably benign	Het
Pdlim4	A	T	11: 53,947,080 (GRCm39)	L132Q	possibly damaging	Het
Ptcd3	T	C	6: 71,875,379 (GRCm39)	D201G	probably benign	Het
Ptk7	A	T	17: 46,897,223 (GRCm39)	F370I	probably benign	Het
Pus7l	T	C	15: 94,431,517 (GRCm39)	N371D	probably benign	Het
Pzp	G	A	6: 128,480,518 (GRCm39)	A589V	probably benign	Het
Rasef	T	A	4: 73,652,786 (GRCm39)	R572W	probably damaging	Het
Reep5	T	C	18: 34,482,712 (GRCm39)	T166A	probably benign	Het
Rhov	A	G	2: 119,101,501 (GRCm39)	V35A	probably damaging	Het
Ripk4	C	T	16: 97,545,097 (GRCm39)	G517R	probably damaging	Het
Rnasel	A	G	1: 153,630,800 (GRCm39)	T439A	probably damaging	Het
Slamf9	A	G	1: 172,304,907 (GRCm39)	T174A	probably benign	Het
Slc12a9	A	G	5: 137,321,411 (GRCm39)	L414P	probably damaging	Het
Slf1	G	A	13: 77,214,767 (GRCm39)	R640*	probably null	Het
Slfn8	G	T	11: 82,907,712 (GRCm39)	P277Q	probably damaging	Het
Stpg2	T	A	3: 139,125,463 (GRCm39)		probably benign	Het
Tm9sf3	A	C	19: 41,211,618 (GRCm39)	N408K	possibly damaging	Het
Trio	A	G	15: 27,758,433 (GRCm39)	V2049A	possibly damaging	Het
Ttn	A	T	2: 76,645,077 (GRCm39)	I11180N	probably damaging	Het
Ush2a	T	C	1: 188,148,018 (GRCm39)	C982R	probably damaging	Het
Usp36	C	T	11: 118,164,392 (GRCm39)	V207M	probably damaging	Het
Uvrag	T	C	7: 98,767,431 (GRCm39)	T67A	probably damaging	Het
Vasp	G	A	7: 18,994,903 (GRCm39)		probably benign	Het
Vmn2r115	T	C	17: 23,578,513 (GRCm39)	F662S	probably damaging	Het
Vmn2r54	A	T	7: 12,366,434 (GRCm39)	C167S	probably damaging	Het
Vmn2r71	C	A	7: 85,270,476 (GRCm39)	N547K	probably damaging	Het
Wasf2	A	G	4: 132,903,902 (GRCm39)	I37V	probably benign	Het
Wwc2	T	C	8: 48,295,937 (GRCm39)	E1111G	unknown	Het
Zfp317	A	G	9: 19,558,608 (GRCm39)	Y274C	probably damaging	Het
Zhx3	T	C	2: 160,623,195 (GRCm39)	Y324C	probably damaging	Het
Zzef1	T	C	11: 72,754,862 (GRCm39)		probably null	Het

Other mutations in Or7a35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00913:Or7a35	APN	10	78,854,085 (GRCm39)	missense	probably benign	0.00
IGL01833:Or7a35	APN	10	78,853,770 (GRCm39)	missense	probably benign
IGL02141:Or7a35	APN	10	78,853,555 (GRCm39)	missense	probably damaging	1.00
IGL02178:Or7a35	APN	10	78,853,554 (GRCm39)	missense	possibly damaging	0.59
R1070:Or7a35	UTSW	10	78,853,684 (GRCm39)	missense	probably damaging	0.99
R1631:Or7a35	UTSW	10	78,853,239 (GRCm39)	missense	probably benign	0.00
R1781:Or7a35	UTSW	10	78,853,159 (GRCm39)	start codon destroyed	probably null	0.89
R2358:Or7a35	UTSW	10	78,854,022 (GRCm39)	missense	probably damaging	1.00
R3161:Or7a35	UTSW	10	78,853,438 (GRCm39)	missense	probably benign
R3162:Or7a35	UTSW	10	78,853,438 (GRCm39)	missense	probably benign
R3162:Or7a35	UTSW	10	78,853,438 (GRCm39)	missense	probably benign
R5874:Or7a35	UTSW	10	78,853,191 (GRCm39)	missense	possibly damaging	0.95
R6382:Or7a35	UTSW	10	78,853,351 (GRCm39)	missense	probably damaging	1.00
R7108:Or7a35	UTSW	10	78,853,483 (GRCm39)	nonsense	probably null
R7567:Or7a35	UTSW	10	78,853,379 (GRCm39)	missense	probably benign	0.04
R7568:Or7a35	UTSW	10	78,853,341 (GRCm39)	missense	probably benign
R7915:Or7a35	UTSW	10	78,853,264 (GRCm39)	missense	probably damaging	1.00
R7936:Or7a35	UTSW	10	78,853,872 (GRCm39)	missense	probably damaging	1.00
R9687:Or7a35	UTSW	10	78,853,843 (GRCm39)	missense	probably damaging	1.00
Z1176:Or7a35	UTSW	10	78,854,039 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACTGAGCCCTTCAAATTGAGCC -3'
(R):5'- GTCAGCAGAAAGATGTCCAGTACCG -3'

Sequencing Primer
(F):5'- GCGTGTCAAACACATGGAAC -3'
(R):5'- GATGTCCAGTACCGAAAAAAGTAG -3'

Posted On

2014-04-24