|Institutional Source||Beutler Lab|
|Gene Name||CD86 antigen|
|Synonyms||B70, B7.2, Ly58, Cd28l2, Ly-58, MB7-2, B7-2|
|Is this an essential gene?||Probably non essential (E-score: 0.036)|
|Stock #||R1616 (G1)|
|Chromosomal Location||36603869-36666081 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 36628976 bp|
|Amino Acid Change||Isoleucine to Valine at position 20 (I20V)|
|Ref Sequence||ENSEMBL: ENSMUSP00000087047 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000089620] [ENSMUST00000135280]|
|Predicted Effect||probably benign
AA Change: I20V
PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)
AA Change: I20V
|Predicted Effect||noncoding transcript
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
PHENOTYPE: Homozygous null mice on an NOD background display a phenotype similar to human Guillain-Barre Syndrome, exhibiting severe peripheral nervous system inflammation, sciatic nerve demyelination, elevated auto-antibodies to myelin protein zero, hindlimb paralysis, and weak forelimb grip. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cd86||
(F):5'- AGCTATTTGCTATGGAGTCTTCGGC -3'
(R):5'- GGCTAATGTCACAGTCTCGATCAGC -3'
(F):5'- TATGGAGTCTTCGGCAACTCAAG -3'
(R):5'- ggggagggagaagaggg -3'