Incidental Mutation 'R1603:Kras'
ID 176250
Institutional Source Beutler Lab
Gene Symbol Kras
Ensembl Gene ENSMUSG00000030265
Gene Name Kirsten rat sarcoma viral oncogene homolog
Synonyms Kras2, Kras-2, K-ras, Ki-ras
MMRRC Submission 039640-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R1603 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 145162425-145195965 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) A to T at 145170871 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Leucine to Stop codon at position 168 (L168*)
Ref Sequence ENSEMBL: ENSMUSP00000107339 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032399] [ENSMUST00000111710] [ENSMUST00000156486] [ENSMUST00000203147]
AlphaFold P32883
Predicted Effect probably benign
Transcript: ENSMUST00000032399
SMART Domains Protein: ENSMUSP00000032399
Gene: ENSMUSG00000030265

DomainStartEndE-ValueType
RAS 1 166 1.14e-123 SMART
Predicted Effect probably null
Transcript: ENSMUST00000111710
AA Change: L168*
SMART Domains Protein: ENSMUSP00000107339
Gene: ENSMUSG00000030265
AA Change: L168*

DomainStartEndE-ValueType
RAS 1 166 3.7e-123 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000123972
Predicted Effect noncoding transcript
Transcript: ENSMUST00000149314
Predicted Effect probably benign
Transcript: ENSMUST00000156486
Predicted Effect probably benign
Transcript: ENSMUST00000203147
SMART Domains Protein: ENSMUSP00000145294
Gene: ENSMUSG00000030265

DomainStartEndE-ValueType
small_GTPase 1 53 3.1e-8 SMART
Meta Mutation Damage Score 0.9754 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.1%
  • 10x: 95.4%
  • 20x: 89.3%
Validation Efficiency 96% (65/68)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality, decreased fetal growth, pericardial edema, anemia, and liver hypoplasia. Mice heterozygous for various knock-in alleles exhibit increased tumorigenesis. [provided by MGI curators]
Allele List at MGI

All alleles(26) : Targeted, knock-out(3) Targeted, other(7) Gene trapped(14) Other(2)
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110032F04Rik G A 3: 68,777,546 (GRCm39) S169N probably benign Het
Ace T A 11: 105,862,925 (GRCm39) S315R probably benign Het
Adamtsl1 A G 4: 86,333,767 (GRCm39) I1491V probably benign Het
Adrb2 A T 18: 62,312,579 (GRCm39) M82K probably damaging Het
Aebp2 A G 6: 140,587,979 (GRCm39) N350D probably damaging Het
Ankrd42 T C 7: 92,268,899 (GRCm39) probably benign Het
Asap1 A T 15: 64,001,106 (GRCm39) C492S probably damaging Het
Atp8b3 C A 10: 80,361,619 (GRCm39) A768S probably benign Het
Chga T A 12: 102,530,866 (GRCm39) probably null Het
Clca4b A G 3: 144,627,780 (GRCm39) V397A probably benign Het
Cntnap5a A C 1: 116,339,831 (GRCm39) T697P possibly damaging Het
Col12a1 T C 9: 79,520,244 (GRCm39) Q2810R probably damaging Het
Dchs1 G T 7: 105,411,977 (GRCm39) R1380S probably benign Het
Dgkg T A 16: 22,388,909 (GRCm39) probably benign Het
Dlgap3 G A 4: 127,089,021 (GRCm39) G206R probably damaging Het
Dnah5 A G 15: 28,295,131 (GRCm39) probably benign Het
Dnah5 A T 15: 28,449,326 (GRCm39) I4243L probably benign Het
Eif1ad16 C A 12: 87,985,134 (GRCm39) E136D probably benign Het
Fbxw22 G T 9: 109,207,915 (GRCm39) P452H probably benign Het
Fgl1 T A 8: 41,650,055 (GRCm39) D242V probably damaging Het
Gba2 C T 4: 43,567,823 (GRCm39) G794R probably damaging Het
Gimap7 A T 6: 48,700,864 (GRCm39) D150V probably damaging Het
Gm5334 T C 7: 68,268,620 (GRCm39) V13A probably benign Het
Grk5 T C 19: 61,057,800 (GRCm39) F167L probably benign Het
Ice1 A G 13: 70,751,472 (GRCm39) L1538P probably benign Het
Idh2 T G 7: 79,748,906 (GRCm39) E125A probably damaging Het
Kmt2a A T 9: 44,752,858 (GRCm39) probably null Het
Lrrc66 G A 5: 73,764,769 (GRCm39) S758L possibly damaging Het
Mak T C 13: 41,195,582 (GRCm39) D377G possibly damaging Het
Matn2 T C 15: 34,388,914 (GRCm39) C335R probably damaging Het
Mcoln2 C T 3: 145,885,977 (GRCm39) S276F probably damaging Het
Morc3 C A 16: 93,663,391 (GRCm39) N531K probably benign Het
Obp2a G A 2: 25,592,757 (GRCm39) S175N probably benign Het
Or2ak6 T C 11: 58,593,286 (GRCm39) V253A probably benign Het
Or4n4b T G 14: 50,536,491 (GRCm39) I92L possibly damaging Het
Or5an1b A T 19: 12,299,348 (GRCm39) V281E probably damaging Het
Osbpl3 C A 6: 50,300,073 (GRCm39) K510N probably damaging Het
Pcnx2 G A 8: 126,566,365 (GRCm39) S1026F probably damaging Het
Pom121l2 A T 13: 22,167,514 (GRCm39) D595V probably damaging Het
Poteg T A 8: 27,938,033 (GRCm39) M1K probably null Het
Rbl1 G A 2: 157,017,579 (GRCm39) L547F possibly damaging Het
Rpap3 T C 15: 97,599,002 (GRCm39) T82A possibly damaging Het
Sema7a G A 9: 57,867,959 (GRCm39) D512N probably benign Het
Sgpl1 C T 10: 60,941,230 (GRCm39) V294M possibly damaging Het
Slc22a28 A G 19: 8,040,674 (GRCm39) S526P probably damaging Het
Spata31e2 T A 1: 26,724,650 (GRCm39) R177W probably damaging Het
Tent2 G A 13: 93,312,073 (GRCm39) A209V probably benign Het
Trim30b T G 7: 104,015,019 (GRCm39) Q123P possibly damaging Het
Trpm5 A G 7: 142,638,946 (GRCm39) L275P probably benign Het
Ttc33 A G 15: 5,219,275 (GRCm39) E71G probably damaging Het
Unc13d T C 11: 115,964,481 (GRCm39) T288A possibly damaging Het
Unc93a T A 17: 13,328,521 (GRCm39) E444V probably benign Het
Usf3 T A 16: 44,038,535 (GRCm39) M1005K probably benign Het
Vmn2r91 T C 17: 18,326,405 (GRCm39) I230T probably benign Het
Wdr49 G A 3: 75,304,177 (GRCm39) Q448* probably null Het
Zfp939 T A 7: 39,122,695 (GRCm39) noncoding transcript Het
Other mutations in Kras
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00808:Kras APN 6 145,192,474 (GRCm39) missense probably damaging 1.00
IGL02929:Kras APN 6 145,177,815 (GRCm39) intron probably benign
N/A - 293:Kras UTSW 6 145,177,940 (GRCm39) missense probably benign 0.01
R1463:Kras UTSW 6 145,170,787 (GRCm39) intron probably benign
R1518:Kras UTSW 6 145,177,977 (GRCm39) missense probably benign 0.00
R1885:Kras UTSW 6 145,177,843 (GRCm39) missense probably damaging 1.00
R5089:Kras UTSW 6 145,170,869 (GRCm39) missense probably benign 0.00
R5133:Kras UTSW 6 145,177,879 (GRCm39) missense probably benign 0.00
R7710:Kras UTSW 6 145,166,354 (GRCm39) missense probably benign
R7876:Kras UTSW 6 145,170,848 (GRCm39) missense probably benign
R8151:Kras UTSW 6 145,166,360 (GRCm39) small deletion probably benign
R8944:Kras UTSW 6 145,170,853 (GRCm39) missense probably benign
R8951:Kras UTSW 6 145,166,338 (GRCm39) missense probably benign
R9345:Kras UTSW 6 145,192,442 (GRCm39) missense probably benign 0.00
Z1177:Kras UTSW 6 145,192,498 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TGCAGATCAGTCTACTGTAGCCATCAC -3'
(R):5'- ACCAGGTTCTAGCTCATGTTTTAACGC -3'

Sequencing Primer
(F):5'- GTCTACTGTAGCCATCACACAGG -3'
(R):5'- AGCTCATGTTTTAACGCTGCATC -3'
Posted On 2014-04-24