Incidental Mutation 'F6893:Olfr161'
Institutional Source Beutler Lab
Gene Symbol Olfr161
Ensembl Gene ENSMUSG00000051003
Gene Nameolfactory receptor 161
SynonymsMOR131-1, GA_x54KRFPKG5P-112942-113883
Accession Numbers

Genbank: NM_146860; MGI: 3032605

Is this an essential gene? Possibly non essential (E-score: 0.366) question?
Stock #F6893 (G3) of strain busy
Quality Score
Status Validated
Chromosomal Location3591042-3599323 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 3593163 bp
Amino Acid Change Isoleucine to Valine at position 256 (I256V)
Ref Sequence ENSEMBL: ENSMUSP00000150825 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000061541] [ENSMUST00000216259]
Predicted Effect possibly damaging
Transcript: ENSMUST00000061541
AA Change: I256V

PolyPhen 2 Score 0.546 (Sensitivity: 0.88; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000058956
Gene: ENSMUSG00000051003
AA Change: I256V

Pfam:7tm_4 32 308 6.4e-60 PFAM
Pfam:7TM_GPCR_Srsx 36 220 7.2e-6 PFAM
Pfam:7tm_1 42 291 2.9e-25 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000216259
AA Change: I256V

PolyPhen 2 Score 0.546 (Sensitivity: 0.88; Specificity: 0.91)
Meta Mutation Damage Score 0.192 question?
Coding Region Coverage
  • 1x: 88.7%
  • 3x: 74.0%
Validation Efficiency 88% (165/188)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 35 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca14 G A 7: 120,325,038 V1638M probably damaging Het
Agrn C T 4: 156,174,179 R972Q probably benign Het
Anxa3 T C 5: 96,824,994 probably benign Het
Bpifa6 G T 2: 153,987,158 D202Y probably damaging Het
Ccdc15 G A 9: 37,315,640 T346I probably damaging Homo
Celsr3 G A 9: 108,835,067 R1731H probably benign Het
Ces4a A G 8: 105,147,227 R443G possibly damaging Het
Chd2 T C 7: 73,507,872 Q175R possibly damaging Het
Dpyd T A 3: 118,804,134 probably null Het
Dscam G T 16: 97,056,460 H117N possibly damaging Het
F13a1 A G 13: 36,972,025 Y205H probably damaging Het
Fat3 A C 9: 16,006,789 L1446R probably damaging Homo
Golga4 T C 9: 118,553,457 L515S probably damaging Het
Hoxb1 A T 11: 96,365,902 T26S probably benign Het
Igsf10 T G 3: 59,331,060 T567P probably damaging Het
Lamb2 T C 9: 108,482,556 V365A probably benign Het
Mepe A G 5: 104,337,376 I127M possibly damaging Het
Mpi A T 9: 57,546,549 M230K probably benign Homo
Myh4 A G 11: 67,255,457 D1447G probably null Homo
Olfr350 A G 2: 36,850,807 T254A probably benign Het
Panx2 T C 15: 89,068,010 Y227H probably damaging Homo
Pdzd7 A G 19: 45,036,734 W441R probably damaging Het
Poldip2 A G 11: 78,519,194 I267M probably damaging Homo
Pros1 T A 16: 62,924,639 V539E probably damaging Het
Sacs T C 14: 61,212,976 M4157T probably benign Het
Slc45a3 A G 1: 131,981,337 E424G probably benign Homo
Slc9a1 A G 4: 133,422,146 E761G probably benign Homo
Stab2 G A 10: 86,855,171 P2178L probably damaging Het
Syt4 C T 18: 31,444,221 V27I possibly damaging Homo
Thumpd1 T A 7: 119,720,576 K56* probably null Het
Tpr A G 1: 150,393,562 K19E possibly damaging Homo
Ttll10 A G 4: 156,048,318 I74T probably benign Het
Txnrd1 C T 10: 82,866,989 Q95* probably null Homo
Zc3h7b A G 15: 81,778,671 E421G possibly damaging Homo
Zc3hc1 G T 6: 30,387,526 D51E probably benign Homo
Other mutations in Olfr161
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01447:Olfr161 APN 16 3592984 missense possibly damaging 0.55
IGL01519:Olfr161 APN 16 3592534 missense probably damaging 1.00
IGL02727:Olfr161 APN 16 3593326 missense probably benign 0.04
IGL03278:Olfr161 APN 16 3593107 missense possibly damaging 0.55
R1634:Olfr161 UTSW 16 3593209 missense probably benign 0.19
R2345:Olfr161 UTSW 16 3593139 missense probably damaging 0.99
R4858:Olfr161 UTSW 16 3592842 missense probably damaging 1.00
R4930:Olfr161 UTSW 16 3592435 missense probably damaging 1.00
R6774:Olfr161 UTSW 16 3592516 missense probably damaging 1.00
Nature of Mutation
DNA sequencing using the SOLiD technique identified an A to G transition at position 766 of the Olfr161 transcript.  The mutated nucleotide causes an isoleucine to valine substitution at amino acid 256 of the encoded protein. The mutation has been confirmed by DNA sequencing using the Sanger method (Figure 1).
Protein Function and Prediction
The Olfr161 gene encodes the 313 amino acid olfactory receptor 161. Olfactory receptors are G-protein coupled receptors (GPCRs). Like other GPCRs, OLFR161 contains seven transmembrane domains, an extracellular N-terminus, and a cytoplasmic C-terminus (Uniprot Q8VGB9). 
The I256V change is predicted to be benign by the PolyPhen program.
Posted On2010-05-03