Mutagenetix > Incidental Mutation

Incidental Mutation 'R1669:Itgb4'

187470

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

MMRRC Submission

039705-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1669 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

115865556-115899238 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 115882156 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 825 (R825H)

Ref Sequence

ENSEMBL: ENSMUSP00000102069 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

AlphaFold

A2A863

Predicted Effect

probably benign
Transcript: ENSMUST00000021107
AA Change: R824H

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: R824H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000068981
AA Change: R825H

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: R825H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106458
AA Change: R825H

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: R825H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106460
AA Change: R825H

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: R825H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106461
AA Change: R825H

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: R825H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127523

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130286

Predicted Effect

probably benign
Transcript: ENSMUST00000169928
AA Change: R824H

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: R824H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151691

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150129

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180782

Meta Mutation Damage Score

0.1672

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.3%

Validation Efficiency

99% (85/86)

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano10	A	C	9: 122,086,249 (GRCm39)	L258R	possibly damaging	Het
Ap3d1	A	G	10: 80,546,670 (GRCm39)		probably benign	Het
Aplp2	G	T	9: 31,079,029 (GRCm39)		probably benign	Het
Arhgap11a	T	C	2: 113,672,257 (GRCm39)	D237G	possibly damaging	Het
Arhgef4	A	T	1: 34,771,239 (GRCm39)	H1182L	possibly damaging	Het
Ash1l	G	A	3: 88,974,549 (GRCm39)		probably null	Het
Bicdl1	C	A	5: 115,794,075 (GRCm39)	V224L	possibly damaging	Het
Cacna1h	C	A	17: 25,602,445 (GRCm39)	V1422L	probably damaging	Het
Card10	T	C	15: 78,678,153 (GRCm39)	E365G	probably benign	Het
Cd180	C	T	13: 102,841,998 (GRCm39)	T348I	probably damaging	Het
Cds2	T	A	2: 132,137,439 (GRCm39)		probably null	Het
Chaf1a	C	A	17: 56,370,339 (GRCm39)	D601E	probably benign	Het
Coprs	C	T	8: 13,935,704 (GRCm39)	W12*	probably null	Het
Cpsf3	T	A	12: 21,355,332 (GRCm39)	M424K	probably damaging	Het
Cxcl13	T	A	5: 96,106,600 (GRCm39)	N57K	probably damaging	Het
Dact1	C	T	12: 71,365,547 (GRCm39)	T776M	probably damaging	Het
Depdc1a	A	G	3: 159,228,561 (GRCm39)	K438E	probably benign	Het
Dop1b	T	A	16: 93,566,548 (GRCm39)	S992T	probably damaging	Het
Dusp11	A	T	6: 85,927,008 (GRCm39)	H202Q	probably benign	Het
Ezr	T	C	17: 7,006,712 (GRCm39)	E584G	probably damaging	Het
F830104G03Rik	A	G	3: 56,797,998 (GRCm39)	V6A	unknown	Het
Fam227a	G	A	15: 79,504,878 (GRCm39)		probably null	Het
Gapvd1	T	G	2: 34,620,694 (GRCm39)		probably null	Het
Gm10267	T	C	18: 44,290,367 (GRCm39)	R47G	probably damaging	Het
Hectd3	T	A	4: 116,856,840 (GRCm39)	D462E	probably damaging	Het
Hfe	A	T	13: 23,890,110 (GRCm39)	L133*	probably null	Het
Hpcal4	T	C	4: 123,082,869 (GRCm39)	F72L	probably damaging	Het
Kcnh8	T	A	17: 53,200,996 (GRCm39)	Y477N	probably damaging	Het
Kctd1	T	C	18: 15,195,517 (GRCm39)	N369D	possibly damaging	Het
Kdm1b	C	T	13: 47,222,024 (GRCm39)	R488C	probably damaging	Het
Kif1c	C	T	11: 70,619,223 (GRCm39)	L953F	probably damaging	Het
Klra8	A	T	6: 130,092,592 (GRCm39)	C236*	probably null	Het
Lca5l	T	C	16: 95,961,008 (GRCm39)	R485G	possibly damaging	Het
Lrrc2	A	G	9: 110,810,718 (GRCm39)	T363A	probably damaging	Het
Lsmem1	GTACATACATACATACATACATACATACA	GTACATACATACATACATACATACATACATACA	12: 40,235,260 (GRCm39)		probably null	Het
Lyst	G	A	13: 13,818,672 (GRCm39)	G1314D	possibly damaging	Het
Mmp10	A	T	9: 7,505,526 (GRCm39)		probably null	Het
Mmp13	A	G	9: 7,277,926 (GRCm39)	D273G	probably benign	Het
Morc2a	G	A	11: 3,625,885 (GRCm39)	V162M	probably benign	Het
Msh4	C	T	3: 153,582,357 (GRCm39)	R417Q	possibly damaging	Het
Nedd9	A	T	13: 41,465,270 (GRCm39)	V790E	probably damaging	Het
Nlgn1	C	A	3: 25,490,298 (GRCm39)	L476F	probably damaging	Het
Nox4	C	A	7: 86,945,097 (GRCm39)	Q118K	probably benign	Het
Nxf1	A	G	19: 8,749,495 (GRCm39)	T131A	possibly damaging	Het
Oog3	C	A	4: 143,885,008 (GRCm39)	R309S	probably benign	Het
Oosp3	C	T	19: 11,678,378 (GRCm39)		probably benign	Het
Or10a3	T	A	7: 108,479,920 (GRCm39)	T298S	probably benign	Het
Or2a7	T	A	6: 43,151,755 (GRCm39)	S278R	probably damaging	Het
Or2w6	A	T	13: 21,843,456 (GRCm39)	S12R	possibly damaging	Het
Or4c126	T	A	2: 89,824,144 (GRCm39)		probably null	Het
Or51a42	A	T	7: 103,708,516 (GRCm39)	S98T	probably benign	Het
Or5b97	A	T	19: 12,878,652 (GRCm39)	I164K	possibly damaging	Het
P2ry6	A	G	7: 100,587,630 (GRCm39)	V243A	probably damaging	Het
Pgc	C	A	17: 48,044,715 (GRCm39)	P321T	probably damaging	Het
Pramel22	T	G	4: 143,380,916 (GRCm39)	E369A	possibly damaging	Het
Prkd1	G	A	12: 50,441,709 (GRCm39)	H277Y	probably damaging	Het
Prkdc	T	A	16: 15,551,922 (GRCm39)	S2043T	probably damaging	Het
Rasal3	T	C	17: 32,622,072 (GRCm39)	N96D	possibly damaging	Het
Rnf4	T	C	5: 34,508,624 (GRCm39)	F162S	probably damaging	Het
Ros1	A	T	10: 52,037,907 (GRCm39)	N421K	probably damaging	Het
Rtn4rl1	A	G	11: 75,156,753 (GRCm39)	D395G	probably benign	Het
Scn8a	T	G	15: 100,909,001 (GRCm39)	V823G	probably damaging	Het
Sema3d	T	A	5: 12,558,051 (GRCm39)		probably benign	Het
Slc12a2	T	C	18: 58,037,307 (GRCm39)	I501T	probably damaging	Het
Slc12a7	C	T	13: 73,943,232 (GRCm39)	T382I	probably benign	Het
Slc16a12	T	A	19: 34,657,781 (GRCm39)	I41L	probably benign	Het
Slc1a1	C	A	19: 28,889,194 (GRCm39)	T489K	probably benign	Het
Slc24a3	C	T	2: 145,455,512 (GRCm39)	P467L	probably damaging	Het
Slco1a8	T	A	6: 141,933,415 (GRCm39)	I457F	probably benign	Het
Snai2	A	T	16: 14,524,908 (GRCm39)	Y138F	possibly damaging	Het
Sorcs1	A	C	19: 50,463,860 (GRCm39)	Y197D	probably damaging	Het
Sppl2a	T	A	2: 126,759,714 (GRCm39)		probably benign	Het
Srgap3	G	A	6: 112,699,865 (GRCm39)	P1038S	probably benign	Het
Ttn	T	C	2: 76,554,807 (GRCm39)	E30699G	probably damaging	Het
Vmn2r124	T	A	17: 18,283,206 (GRCm39)	M300K	possibly damaging	Het
Vwf	T	C	6: 125,624,869 (GRCm39)	S1873P	possibly damaging	Het
Wrap73	A	G	4: 154,240,588 (GRCm39)	D360G	probably damaging	Het
Zfp366	T	C	13: 99,366,069 (GRCm39)	M410T	probably damaging	Het
Zfp608	C	T	18: 55,120,811 (GRCm39)	V259I	probably benign	Het
Zfp69	C	T	4: 120,804,695 (GRCm39)		probably benign	Het
Zfp995	T	C	17: 22,098,945 (GRCm39)	T430A	probably benign	Het

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115,881,766 (GRCm39)	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115,881,746 (GRCm39)	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	115,897,283 (GRCm39)	splice site	probably benign
IGL01750:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	115,894,213 (GRCm39)	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	115,898,795 (GRCm39)	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	115,896,792 (GRCm39)	missense	probably benign
IGL02879:Itgb4	APN	11	115,885,178 (GRCm39)	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115,879,731 (GRCm39)	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115,879,550 (GRCm39)	missense	probably damaging	1.00
IGL03054:Itgb4	UTSW	11	115,891,166 (GRCm39)	nonsense	probably null
R0021:Itgb4	UTSW	11	115,870,453 (GRCm39)	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115,869,950 (GRCm39)	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115,870,238 (GRCm39)	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	115,898,428 (GRCm39)	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115,870,582 (GRCm39)	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115,870,521 (GRCm39)	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115,881,835 (GRCm39)	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115,870,594 (GRCm39)	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115,884,168 (GRCm39)	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115,888,988 (GRCm39)	intron	probably benign
R1381:Itgb4	UTSW	11	115,885,163 (GRCm39)	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115,881,710 (GRCm39)	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115,869,937 (GRCm39)	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115,874,990 (GRCm39)	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115,874,873 (GRCm39)	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115,890,625 (GRCm39)	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115,871,817 (GRCm39)	missense	probably damaging	1.00
R1623:Itgb4	UTSW	11	115,882,142 (GRCm39)	nonsense	probably null
R1633:Itgb4	UTSW	11	115,898,586 (GRCm39)	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	115,898,183 (GRCm39)	missense	probably damaging	1.00
R1713:Itgb4	UTSW	11	115,894,315 (GRCm39)	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115,879,744 (GRCm39)	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115,879,346 (GRCm39)	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115,874,590 (GRCm39)	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115,871,564 (GRCm39)	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115,884,279 (GRCm39)	nonsense	probably null
R2102:Itgb4	UTSW	11	115,896,561 (GRCm39)	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115,870,450 (GRCm39)	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115,884,261 (GRCm39)	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	115,897,389 (GRCm39)	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	115,894,496 (GRCm39)	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	115,896,752 (GRCm39)	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	115,897,296 (GRCm39)	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115,881,761 (GRCm39)	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115,879,555 (GRCm39)	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115,890,640 (GRCm39)	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115,874,087 (GRCm39)	splice site	silent
R4585:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4601:Itgb4	UTSW	11	115,896,548 (GRCm39)	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	115,897,431 (GRCm39)	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115,879,417 (GRCm39)	intron	probably benign
R5084:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	115,897,365 (GRCm39)	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115,880,575 (GRCm39)	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115,875,894 (GRCm39)	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115,879,258 (GRCm39)	intron	probably benign
R5812:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115,870,212 (GRCm39)	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115,885,102 (GRCm39)	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	115,891,237 (GRCm39)	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115,888,963 (GRCm39)	missense	possibly damaging	0.83
R6258:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6259:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6612:Itgb4	UTSW	11	115,874,897 (GRCm39)	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	115,896,391 (GRCm39)	nonsense	probably null
R7371:Itgb4	UTSW	11	115,888,906 (GRCm39)	missense	probably benign	0.29
R7605:Itgb4	UTSW	11	115,897,302 (GRCm39)	missense	probably benign	0.01
R7659:Itgb4	UTSW	11	115,870,557 (GRCm39)	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	115,894,536 (GRCm39)	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	115,894,510 (GRCm39)	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	115,891,087 (GRCm39)	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.18
R7923:Itgb4	UTSW	11	115,873,525 (GRCm39)	critical splice acceptor site	probably null
R8004:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.00
R8143:Itgb4	UTSW	11	115,884,255 (GRCm39)	missense	probably damaging	1.00
R8427:Itgb4	UTSW	11	115,882,544 (GRCm39)	critical splice donor site	probably null
R8857:Itgb4	UTSW	11	115,871,853 (GRCm39)	missense	probably benign	0.04
R8863:Itgb4	UTSW	11	115,875,898 (GRCm39)	nonsense	probably null
R8932:Itgb4	UTSW	11	115,879,295 (GRCm39)	missense	probably benign	0.01
R9153:Itgb4	UTSW	11	115,874,879 (GRCm39)	missense	probably benign	0.00
R9207:Itgb4	UTSW	11	115,897,923 (GRCm39)	missense	probably damaging	1.00
R9239:Itgb4	UTSW	11	115,898,130 (GRCm39)	missense	probably damaging	1.00
R9267:Itgb4	UTSW	11	115,870,465 (GRCm39)	missense	probably benign
R9289:Itgb4	UTSW	11	115,885,187 (GRCm39)	missense	probably benign	0.01
R9328:Itgb4	UTSW	11	115,880,625 (GRCm39)	missense	probably benign	0.00
R9435:Itgb4	UTSW	11	115,895,855 (GRCm39)	missense	probably benign	0.01
R9450:Itgb4	UTSW	11	115,874,097 (GRCm39)	missense	probably damaging	1.00
R9649:Itgb4	UTSW	11	115,885,171 (GRCm39)	missense	possibly damaging	0.78
R9779:Itgb4	UTSW	11	115,882,485 (GRCm39)	missense	probably damaging	1.00
X0062:Itgb4	UTSW	11	115,884,278 (GRCm39)	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	115,897,346 (GRCm39)	missense	probably damaging	1.00
Z1177:Itgb4	UTSW	11	115,888,884 (GRCm39)	missense	probably benign	0.00
Z1177:Itgb4	UTSW	11	115,877,637 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTCCCATCTTGATGGCACCAGAAC -3'
(R):5'- TGCCTGAGGGATGAACCCTGAAAC -3'

Sequencing Primer
(F):5'- TCTCTGTAGTGCCTTCTGCA -3'
(R):5'- TTCGAAGCACATGTCCAAAGAG -3'

Posted On

2014-05-09