Mutagenetix > Incidental Mutation

Incidental Mutation 'R1679:Cdc25c'

188374

Institutional Source

Beutler Lab

Gene Symbol

Cdc25c

Ensembl Gene

ENSMUSG00000044201

Gene Name

cell division cycle 25C

Synonyms

Cdc25

MMRRC Submission

039715-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1679 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

34866046-34884586 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 34880348 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 129 (T129A)

Ref Sequence

ENSEMBL: ENSMUSP00000055427 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060710]

AlphaFold

P48967

Predicted Effect

probably damaging
Transcript: ENSMUST00000060710
AA Change: T129A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000055427
Gene: ENSMUSG00000044201
AA Change: T129A

Domain	Start	End	E-Value	Type
low complexity region	246	257	N/A	INTRINSIC
RHOD	284	398	3.71e-21	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181453

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181641

Meta Mutation Damage Score

0.7675

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.0%

Validation Efficiency

98% (51/52)

MGI Phenotype

FUNCTION: This gene encodes a dual specificity phosphatase that dephosphorylates cyclin B-bound Cdk1 to trigger entry into mitosis. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation exhibit no discernable phenotype; mice are viable and fertile with normal T and B lymphocyte development and proliferative responses. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921517D22Rik	GCC	GC	13: 59,839,412 (GRCm39)		probably null	Het
Adam6a	A	G	12: 113,508,376 (GRCm39)	M250V	probably benign	Het
Adgre5	C	A	8: 84,456,034 (GRCm39)	R254L	probably benign	Het
Adgrv1	A	T	13: 81,707,671 (GRCm39)	L525Q	probably damaging	Het
Ano9	T	A	7: 140,688,210 (GRCm39)	I205F	probably benign	Het
Bmp5	T	C	9: 75,746,877 (GRCm39)	V245A	probably benign	Het
Capn8	T	C	1: 182,441,032 (GRCm39)	S489P	probably damaging	Het
Ccdc85a	A	G	11: 28,533,316 (GRCm39)	L76P	probably damaging	Het
Cd164l2	A	G	4: 132,948,810 (GRCm39)	T49A	probably benign	Het
Cfap43	T	C	19: 47,761,553 (GRCm39)	D847G	probably benign	Het
Crim1	G	A	17: 78,508,228 (GRCm39)	A11T	probably benign	Het
Cul9	A	T	17: 46,832,082 (GRCm39)	L1449H	possibly damaging	Het
Cyp2a22	T	A	7: 26,635,736 (GRCm39)	K276*	probably null	Het
Cyp2c50	C	A	19: 40,099,859 (GRCm39)	T430K	possibly damaging	Het
Ddb2	C	T	2: 91,064,595 (GRCm39)	R105Q	probably benign	Het
Emilin1	T	C	5: 31,077,543 (GRCm39)	Y900H	probably benign	Het
Eml3	T	C	19: 8,914,001 (GRCm39)	F100L	probably damaging	Het
Eps8l2	G	A	7: 140,940,970 (GRCm39)	G542D	probably damaging	Het
Fbf1	A	G	11: 116,041,843 (GRCm39)		probably null	Het
Gm21886	T	C	18: 80,132,954 (GRCm39)	Y68C	probably damaging	Het
H2-Q10	A	G	17: 35,784,492 (GRCm39)		probably benign	Het
Hebp2	G	T	10: 18,420,163 (GRCm39)	T90K	possibly damaging	Het
Il1rl2	A	G	1: 40,382,320 (GRCm39)	T211A	probably benign	Het
Incenp	T	C	19: 9,872,778 (GRCm39)	D16G	unknown	Het
Isg20l2	T	A	3: 87,839,392 (GRCm39)	M201K	probably damaging	Het
Kansl1	A	T	11: 104,314,822 (GRCm39)	S405R	probably damaging	Het
Kdsr	T	A	1: 106,680,956 (GRCm39)	I81F	probably benign	Het
Leprotl1	T	G	8: 34,607,986 (GRCm39)	L7F	probably damaging	Het
Lonrf2	G	A	1: 38,852,357 (GRCm39)	P165S	probably benign	Het
Mtmr2	T	A	9: 13,700,373 (GRCm39)	I60K	probably damaging	Het
Mto1	A	G	9: 78,372,245 (GRCm39)	T572A	probably benign	Het
Nipbl	G	A	15: 8,332,396 (GRCm39)	T2287I	probably benign	Het
Nutm2	A	G	13: 50,623,422 (GRCm39)	T40A	probably benign	Het
Nxf1	T	C	19: 8,746,438 (GRCm39)	S550P	probably benign	Het
Or5p4	A	T	7: 107,680,859 (GRCm39)	N286I	probably damaging	Het
Phactr1	G	A	13: 43,210,756 (GRCm39)	V193I	possibly damaging	Het
Phactr1	A	T	13: 43,248,257 (GRCm39)	Y317F	possibly damaging	Het
Pih1d1	T	C	7: 44,809,250 (GRCm39)		probably null	Het
Rictor	T	C	15: 6,797,571 (GRCm39)	I309T	possibly damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Ruvbl2	T	C	7: 45,074,391 (GRCm39)	D216G	probably damaging	Het
Slc20a2	T	G	8: 23,028,846 (GRCm39)	S106A	possibly damaging	Het
Srprb	A	G	9: 103,069,406 (GRCm39)		probably benign	Het
Stx11	A	T	10: 12,817,580 (GRCm39)	I48N	probably damaging	Het
Vmn1r29	A	T	6: 58,285,003 (GRCm39)	Y241F	probably damaging	Het
Wdfy1	A	T	1: 79,685,192 (GRCm39)	C347*	probably null	Het
Zfp683	T	C	4: 133,785,956 (GRCm39)	V361A	possibly damaging	Het

Other mutations in Cdc25c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00773:Cdc25c	APN	18	34,880,294 (GRCm39)	missense	probably benign	0.45
IGL01357:Cdc25c	APN	18	34,867,910 (GRCm39)	splice site	probably null
IGL02122:Cdc25c	APN	18	34,877,038 (GRCm39)	missense	probably benign	0.03
R0053:Cdc25c	UTSW	18	34,868,488 (GRCm39)	missense	probably benign	0.16
R0053:Cdc25c	UTSW	18	34,868,488 (GRCm39)	missense	probably benign	0.16
R1077:Cdc25c	UTSW	18	34,882,026 (GRCm39)	splice site	probably benign
R2036:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R2051:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R2077:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R2511:Cdc25c	UTSW	18	34,871,292 (GRCm39)	missense	probably damaging	1.00
R5304:Cdc25c	UTSW	18	34,883,864 (GRCm39)	missense	possibly damaging	0.71
R5604:Cdc25c	UTSW	18	34,866,701 (GRCm39)	missense	probably damaging	1.00
R7833:Cdc25c	UTSW	18	34,880,296 (GRCm39)	missense	probably benign	0.17
R7919:Cdc25c	UTSW	18	34,868,429 (GRCm39)	missense	probably damaging	0.99
R8193:Cdc25c	UTSW	18	34,882,675 (GRCm39)	splice site	probably null
R8710:Cdc25c	UTSW	18	34,882,666 (GRCm39)	critical splice acceptor site	probably benign
R8960:Cdc25c	UTSW	18	34,866,329 (GRCm39)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- CAAGGCAGGGGTATAGCTCAATGAC -3'
(R):5'- TGCAGCCGTTGAACACTGAGAC -3'

Sequencing Primer
(F):5'- ccattacaggaacctacactcac -3'
(R):5'- AGACGGAAGCCATGTGCC -3'

Posted On

2014-05-09