Incidental Mutation 'R1402:Zfp663'
| ID |
188566 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp663
|
| Ensembl Gene |
ENSMUSG00000056824 |
| Gene Name |
zinc finger protein 663 |
| Synonyms |
LOC381405, Gm1008 |
| MMRRC Submission |
039464-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.068)
|
| Stock # |
R1402 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
165193217-165210649 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 165195890 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Lysine to Glutamic Acid
at position 110
(K110E)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000099374
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000073062]
[ENSMUST00000103085]
[ENSMUST00000141140]
|
| AlphaFold |
Q6NXM6 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000073062
AA Change: K110E
PolyPhen 2
Score 0.145 (Sensitivity: 0.92; Specificity: 0.87)
|
| SMART Domains |
Protein: ENSMUSP00000072813
Gene: ENSMUSG00000056824
AA Change: K110E
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
68 |
1.97e-31 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
3.47e1 |
SMART |
|
ZnF_C2H2
|
472 |
494 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
500 |
522 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
528 |
550 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
556 |
578 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
584 |
606 |
3.95e-4 |
SMART |
|
ZnF_C2H2
|
612 |
635 |
8.6e-5 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000103085
AA Change: K110E
PolyPhen 2
Score 0.145 (Sensitivity: 0.92; Specificity: 0.87)
|
| SMART Domains |
Protein: ENSMUSP00000099374
Gene: ENSMUSG00000056824
AA Change: K110E
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
68 |
1.97e-31 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
3.47e1 |
SMART |
|
ZnF_C2H2
|
472 |
494 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
500 |
522 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
528 |
550 |
2.43e-4 |
SMART |
|
ZnF_C2H2
|
556 |
578 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
584 |
606 |
3.95e-4 |
SMART |
|
ZnF_C2H2
|
612 |
635 |
8.6e-5 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000136482
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000141140
|
| SMART Domains |
Protein: ENSMUSP00000115254
Gene: ENSMUSG00000056824
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
8 |
68 |
1.97e-31 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.2%
- 10x: 95.9%
- 20x: 91.2%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 21 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aqp8 |
T |
A |
7: 123,065,862 (GRCm39) |
V219E |
probably damaging |
Het |
| Birc6 |
T |
A |
17: 75,004,528 (GRCm39) |
|
probably benign |
Het |
| Bves |
C |
T |
10: 45,223,961 (GRCm39) |
T207M |
probably damaging |
Het |
| Dlg5 |
T |
C |
14: 24,226,676 (GRCm39) |
S409G |
probably benign |
Het |
| Ehmt2 |
C |
T |
17: 35,125,757 (GRCm39) |
T607I |
probably benign |
Het |
| H2-T22 |
A |
T |
17: 36,351,161 (GRCm39) |
I307N |
possibly damaging |
Het |
| Itih3 |
T |
C |
14: 30,630,665 (GRCm39) |
D882G |
probably damaging |
Het |
| Kmt5c |
G |
T |
7: 4,745,252 (GRCm39) |
R81L |
possibly damaging |
Het |
| Mcm3ap |
C |
T |
10: 76,313,748 (GRCm39) |
|
probably benign |
Het |
| Nckap1 |
C |
T |
2: 80,348,286 (GRCm39) |
S889N |
probably benign |
Het |
| Nr1i2 |
A |
G |
16: 38,073,245 (GRCm39) |
S244P |
probably damaging |
Het |
| Pcx |
A |
G |
19: 4,652,058 (GRCm39) |
D101G |
possibly damaging |
Het |
| Prkch |
T |
C |
12: 73,632,163 (GRCm39) |
V76A |
probably damaging |
Het |
| Skic3 |
A |
G |
13: 76,279,533 (GRCm39) |
Y655C |
probably damaging |
Het |
| Thsd1 |
A |
G |
8: 22,749,384 (GRCm39) |
K691E |
possibly damaging |
Het |
| Tmprss11e |
G |
A |
5: 86,863,477 (GRCm39) |
T196I |
probably damaging |
Het |
| Trappc13 |
A |
G |
13: 104,286,624 (GRCm39) |
V211A |
probably damaging |
Het |
| Vav1 |
C |
A |
17: 57,610,849 (GRCm39) |
L472I |
probably benign |
Het |
| Wdr25 |
G |
A |
12: 108,992,465 (GRCm39) |
E459K |
probably damaging |
Het |
| Zdbf2 |
A |
T |
1: 63,342,786 (GRCm39) |
E388D |
possibly damaging |
Het |
| Zfp78 |
C |
A |
7: 6,381,618 (GRCm39) |
H223N |
probably damaging |
Het |
|
| Other mutations in Zfp663 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00720:Zfp663
|
APN |
2 |
165,194,525 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01382:Zfp663
|
APN |
2 |
165,200,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02007:Zfp663
|
APN |
2 |
165,200,993 (GRCm39) |
missense |
probably benign |
0.12 |
|
IGL02164:Zfp663
|
APN |
2 |
165,200,968 (GRCm39) |
nonsense |
probably null |
|
|
IGL02506:Zfp663
|
APN |
2 |
165,195,871 (GRCm39) |
missense |
probably benign |
0.35 |
|
IGL03173:Zfp663
|
APN |
2 |
165,194,419 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0735:Zfp663
|
UTSW |
2 |
165,200,995 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R1395:Zfp663
|
UTSW |
2 |
165,194,492 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1402:Zfp663
|
UTSW |
2 |
165,195,890 (GRCm39) |
missense |
probably benign |
0.14 |
|
R1503:Zfp663
|
UTSW |
2 |
165,194,573 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1587:Zfp663
|
UTSW |
2 |
165,195,437 (GRCm39) |
missense |
probably benign |
|
|
R1854:Zfp663
|
UTSW |
2 |
165,195,211 (GRCm39) |
missense |
probably benign |
0.18 |
|
R1867:Zfp663
|
UTSW |
2 |
165,194,651 (GRCm39) |
missense |
possibly damaging |
0.74 |
|
R3031:Zfp663
|
UTSW |
2 |
165,195,616 (GRCm39) |
nonsense |
probably null |
|
|
R4643:Zfp663
|
UTSW |
2 |
165,194,925 (GRCm39) |
missense |
probably benign |
0.24 |
|
R4691:Zfp663
|
UTSW |
2 |
165,201,050 (GRCm39) |
intron |
probably benign |
|
|
R4977:Zfp663
|
UTSW |
2 |
165,195,731 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5135:Zfp663
|
UTSW |
2 |
165,195,590 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5151:Zfp663
|
UTSW |
2 |
165,195,113 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5639:Zfp663
|
UTSW |
2 |
165,194,929 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5763:Zfp663
|
UTSW |
2 |
165,200,355 (GRCm39) |
nonsense |
probably null |
|
|
R6776:Zfp663
|
UTSW |
2 |
165,200,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6929:Zfp663
|
UTSW |
2 |
165,195,178 (GRCm39) |
missense |
probably benign |
|
|
R6998:Zfp663
|
UTSW |
2 |
165,195,922 (GRCm39) |
missense |
possibly damaging |
0.74 |
|
R7035:Zfp663
|
UTSW |
2 |
165,195,023 (GRCm39) |
missense |
probably benign |
0.36 |
|
R7169:Zfp663
|
UTSW |
2 |
165,194,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7529:Zfp663
|
UTSW |
2 |
165,194,728 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7790:Zfp663
|
UTSW |
2 |
165,194,453 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8087:Zfp663
|
UTSW |
2 |
165,195,679 (GRCm39) |
missense |
probably benign |
0.20 |
|
R8715:Zfp663
|
UTSW |
2 |
165,194,644 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8934:Zfp663
|
UTSW |
2 |
165,194,714 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8966:Zfp663
|
UTSW |
2 |
165,194,958 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9257:Zfp663
|
UTSW |
2 |
165,195,974 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9278:Zfp663
|
UTSW |
2 |
165,202,010 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
R9524:Zfp663
|
UTSW |
2 |
165,195,607 (GRCm39) |
missense |
probably damaging |
0.99 |
|
RF004:Zfp663
|
UTSW |
2 |
165,200,363 (GRCm39) |
missense |
probably benign |
0.00 |
|
Z1177:Zfp663
|
UTSW |
2 |
165,195,033 (GRCm39) |
missense |
probably damaging |
0.99 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGCTCCAAACGCGATGTCTTCTGC -3'
(R):5'- AGTGCGTCAGAACCCCAGAATCAAG -3'
Sequencing Primer
(F):5'- AGATCTTTCATCAGGCGACC -3'
(R):5'- tgggaggcagaggcagg -3'
|
| Posted On |
2014-05-09 |
Incidental Mutation