Mutagenetix > Incidental Mutation

Incidental Mutation 'R1653:Zfp429'

188877

Institutional Source

Beutler Lab

Gene Symbol

Zfp429

Ensembl Gene

ENSMUSG00000078994

Gene Name

zinc finger protein 429

Synonyms

2810487A22Rik

MMRRC Submission

039689-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R1653 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

67536024-67547938 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 67538043 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 467 (R467H)

Ref Sequence

ENSEMBL: ENSMUSP00000105354 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109732] [ENSMUST00000181071] [ENSMUST00000224684] [ENSMUST00000224825]

AlphaFold

Q7M6Y0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109732
AA Change: R467H

PolyPhen 2 Score 0.872 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000105354
Gene: ENSMUSG00000078994
AA Change: R467H

Domain	Start	End	E-Value	Type
KRAB	15	75	7.16e-34	SMART
ZnF_C2H2	119	141	5.12e1	SMART
ZnF_C2H2	147	169	2.27e-4	SMART
ZnF_C2H2	175	197	1.28e-3	SMART
ZnF_C2H2	203	225	1.56e-2	SMART
ZnF_C2H2	259	281	4.62e1	SMART
ZnF_C2H2	287	309	5.14e-3	SMART
ZnF_C2H2	315	337	6.78e-3	SMART
ZnF_C2H2	343	365	3.11e-2	SMART
ZnF_C2H2	371	393	1.25e-1	SMART
ZnF_C2H2	399	421	6.32e-3	SMART
ZnF_C2H2	427	449	1.47e-3	SMART
ZnF_C2H2	455	477	5.42e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181071

SMART Domains

Protein: ENSMUSP00000137755
Gene: ENSMUSG00000078994

Domain	Start	End	E-Value	Type
KRAB	15	75	7.16e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000224684

Predicted Effect

probably benign
Transcript: ENSMUST00000224825

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225810

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam34	A	T	8: 44,103,682 (GRCm39)	C654*	probably null	Het
Adamts19	T	A	18: 59,023,365 (GRCm39)	N253K	probably benign	Het
Adgrb3	A	G	1: 25,140,584 (GRCm39)	L1162S	probably benign	Het
Ap2b1	T	A	11: 83,237,657 (GRCm39)	Y574N	probably damaging	Het
Atrn	A	G	2: 130,777,544 (GRCm39)	I198V	probably benign	Het
Bcan	T	A	3: 87,901,503 (GRCm39)	I400F	probably damaging	Het
Capn10	A	G	1: 92,874,620 (GRCm39)	Y617C	probably damaging	Het
Capn8	A	G	1: 182,451,516 (GRCm39)	N578D	probably benign	Het
Casd1	T	C	6: 4,624,134 (GRCm39)	L309P	probably benign	Het
Ccser1	T	A	6: 61,288,449 (GRCm39)	I204K	probably benign	Het
Cd276	T	C	9: 58,444,732 (GRCm39)	T80A	probably benign	Het
Cdh3	T	C	8: 107,265,700 (GRCm39)	S248P	probably damaging	Het
Celsr2	T	C	3: 108,320,836 (GRCm39)	T659A	possibly damaging	Het
Col6a4	C	T	9: 105,949,608 (GRCm39)	V676I	probably damaging	Het
Crmp1	T	A	5: 37,443,812 (GRCm39)	V575D	probably damaging	Het
Ep400	G	A	5: 110,841,040 (GRCm39)	Q1795*	probably null	Het
Gcnt3	A	G	9: 69,942,359 (GRCm39)	C70R	probably damaging	Het
Gm12258	T	C	11: 58,749,113 (GRCm39)	I96T	possibly damaging	Het
Gpr183	A	G	14: 122,191,675 (GRCm39)	F282S	probably damaging	Het
Igfals	T	C	17: 25,100,052 (GRCm39)	V381A	probably benign	Het
Irs3	C	A	5: 137,642,783 (GRCm39)	L218F	probably damaging	Het
Kdm5b	T	C	1: 134,530,219 (GRCm39)	F410S	probably damaging	Het
Klc4	T	C	17: 46,942,785 (GRCm39)	Y593C	possibly damaging	Het
Lce1h	T	A	3: 92,670,750 (GRCm39)	Q134L	unknown	Het
Lyst	T	C	13: 13,809,811 (GRCm39)	S494P	probably damaging	Het
Marchf3	A	G	18: 56,944,967 (GRCm39)	M42T	probably benign	Het
Myh7	A	G	14: 55,228,246 (GRCm39)	I250T	probably benign	Het
N4bp1	G	T	8: 87,571,576 (GRCm39)	H807Q	probably benign	Het
Ndn	C	T	7: 61,998,256 (GRCm39)	P34L	probably benign	Het
Nfs1	C	T	2: 155,967,256 (GRCm39)	G44D	probably damaging	Het
Nrg1	C	T	8: 32,308,681 (GRCm39)	R445H	probably damaging	Het
Or14c43	T	C	7: 86,115,420 (GRCm39)	V267A	probably benign	Het
Or4e5	A	T	14: 52,728,229 (GRCm39)	F64Y	probably damaging	Het
Or56a5	T	A	7: 104,793,077 (GRCm39)	D141V	possibly damaging	Het
Pak5	C	T	2: 135,958,807 (GRCm39)	V94M	probably damaging	Het
Pdk1	G	A	2: 71,719,339 (GRCm39)		probably null	Het
Sin3b	G	T	8: 73,468,147 (GRCm39)	V290L	probably benign	Het
Sirt1	T	C	10: 63,157,588 (GRCm39)	T609A	probably benign	Het
Skint5	A	T	4: 113,347,875 (GRCm39)	S1289T	unknown	Het
Slc32a1	A	T	2: 158,456,809 (GRCm39)	H488L	probably benign	Het
Slc35b3	A	G	13: 39,139,774 (GRCm39)	S18P	probably benign	Het
Spaca7	T	A	8: 12,636,501 (GRCm39)	I109K	possibly damaging	Het
Tmem204	A	G	17: 25,299,501 (GRCm39)	L6P	possibly damaging	Het
Tubgcp6	G	A	15: 88,991,645 (GRCm39)	R651C	probably damaging	Het
Vps13b	T	A	15: 35,607,418 (GRCm39)	L1117*	probably null	Het
Wdcp	T	C	12: 4,901,815 (GRCm39)	L557P	probably damaging	Het
Wwp2	T	C	8: 108,210,042 (GRCm39)	F140S	possibly damaging	Het
Zfp747l1	G	T	7: 126,983,652 (GRCm39)	H483Q	possibly damaging	Het
Zfp839	T	A	12: 110,821,684 (GRCm39)	M166K	probably benign	Het
Zfyve9	G	A	4: 108,517,774 (GRCm39)	Q1106*	probably null	Het
Zrsr2-ps1	T	A	11: 22,924,158 (GRCm39)	C311S	probably damaging	Het

Other mutations in Zfp429

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Zfp429	APN	13	67,539,132 (GRCm39)	missense	probably damaging	0.96
IGL01913:Zfp429	APN	13	67,544,793 (GRCm39)	missense	probably damaging	1.00
IGL02343:Zfp429	APN	13	67,538,844 (GRCm39)	missense	probably damaging	0.98
IGL02679:Zfp429	APN	13	67,547,855 (GRCm39)	intron	probably benign
IGL03396:Zfp429	APN	13	67,544,159 (GRCm39)	splice site	probably benign
FR4342:Zfp429	UTSW	13	67,544,769 (GRCm39)	missense	probably benign	0.02
R0012:Zfp429	UTSW	13	67,538,796 (GRCm39)	missense	probably benign	0.01
R1232:Zfp429	UTSW	13	67,538,751 (GRCm39)	missense	possibly damaging	0.47
R1330:Zfp429	UTSW	13	67,544,262 (GRCm39)	splice site	probably null
R1761:Zfp429	UTSW	13	67,544,195 (GRCm39)	missense	probably benign	0.28
R1813:Zfp429	UTSW	13	67,538,505 (GRCm39)	missense	possibly damaging	0.55
R2356:Zfp429	UTSW	13	67,538,746 (GRCm39)	missense	probably benign
R4280:Zfp429	UTSW	13	67,538,914 (GRCm39)	missense	probably damaging	1.00
R4283:Zfp429	UTSW	13	67,538,914 (GRCm39)	missense	probably damaging	1.00
R4464:Zfp429	UTSW	13	67,538,617 (GRCm39)	missense	probably benign	0.13
R4789:Zfp429	UTSW	13	67,538,523 (GRCm39)	missense	probably benign	0.06
R5187:Zfp429	UTSW	13	67,538,959 (GRCm39)	missense	probably damaging	0.99
R5250:Zfp429	UTSW	13	67,538,638 (GRCm39)	missense	probably benign	0.00
R6688:Zfp429	UTSW	13	67,544,249 (GRCm39)	missense	probably damaging	0.98
R6772:Zfp429	UTSW	13	67,538,317 (GRCm39)	missense	probably damaging	1.00
R6989:Zfp429	UTSW	13	67,538,080 (GRCm39)	missense	probably benign	0.00
R7041:Zfp429	UTSW	13	67,538,830 (GRCm39)	missense	probably damaging	1.00
R7101:Zfp429	UTSW	13	67,538,931 (GRCm39)	missense	possibly damaging	0.88
R7593:Zfp429	UTSW	13	67,538,410 (GRCm39)	missense	probably damaging	1.00
R7792:Zfp429	UTSW	13	67,538,558 (GRCm39)	nonsense	probably null
R8500:Zfp429	UTSW	13	67,538,828 (GRCm39)	nonsense	probably null
R8721:Zfp429	UTSW	13	67,538,331 (GRCm39)	missense	probably damaging	0.98
R8891:Zfp429	UTSW	13	67,538,830 (GRCm39)	missense	probably damaging	1.00
R9364:Zfp429	UTSW	13	67,538,531 (GRCm39)	missense	probably benign	0.12
R9554:Zfp429	UTSW	13	67,538,531 (GRCm39)	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- GGATGAACTGTCATGTGTCGATCAGG -3'
(R):5'- ACAAACCCTACAAGTGTGAAGACTGTG -3'

Sequencing Primer
(F):5'- GTCATGTGTCGATCAGGCTATAAC -3'
(R):5'- GGCAGATGTTTCTGCTCATCC -3'

Posted On

2014-05-09