Mutagenetix > Incidental Mutation

Incidental Mutation 'R1686:Adgrg3'

189350

Institutional Source

Beutler Lab

Gene Symbol

Adgrg3

Ensembl Gene

ENSMUSG00000060470

Gene Name

adhesion G protein-coupled receptor G3

Synonyms

Pb99, A030001G24Rik, Gpr97

MMRRC Submission

039719-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1686 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

95744320-95771878 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 95759997 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 72 (N72S)

Ref Sequence

ENSEMBL: ENSMUSP00000051079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051259]

AlphaFold

Q8R0T6

Predicted Effect

probably benign
Transcript: ENSMUST00000051259
AA Change: N72S

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000051079
Gene: ENSMUSG00000060470
AA Change: N72S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
GPS	209	256	3.45e-11	SMART
Pfam:7tm_2	260	509	5.1e-33	PFAM
low complexity region	520	531	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211974

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211994

Predicted Effect

probably benign
Transcript: ENSMUST00000212570

Meta Mutation Damage Score

0.0921

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.9%

Validation Efficiency

100% (85/85)

MGI Phenotype

PHENOTYPE: Homozygous null mice display normal B and T cell development. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017N19Rik	G	A	10: 100,448,722 (GRCm39)	V400I	probably damaging	Het
Abcb1b	A	G	5: 8,848,782 (GRCm39)	N14S	probably damaging	Het
Adamts14	A	G	10: 61,034,439 (GRCm39)	Y1150H	probably benign	Het
Akain1	T	A	17: 69,746,527 (GRCm39)	F3I	possibly damaging	Het
Akr1c21	T	C	13: 4,627,452 (GRCm39)	L182P	probably damaging	Het
Arhgap21	A	T	2: 20,886,659 (GRCm39)	Y12N	probably damaging	Het
Aup1	A	T	6: 83,032,226 (GRCm39)	H131L	probably damaging	Het
Bag6	A	G	17: 35,363,928 (GRCm39)	T812A	possibly damaging	Het
Bmp2k	A	G	5: 97,211,392 (GRCm39)	Y520C	unknown	Het
Calm4	T	G	13: 3,888,302 (GRCm39)	V136G	probably damaging	Het
Catsper2	A	G	2: 121,230,523 (GRCm39)		probably null	Het
Cc2d2a	A	G	5: 43,896,713 (GRCm39)	T1537A	possibly damaging	Het
Cntn2	A	T	1: 132,454,049 (GRCm39)	V319D	possibly damaging	Het
Cux1	G	A	5: 136,304,235 (GRCm39)	R1314*	probably null	Het
Cxcl12	A	G	6: 117,150,508 (GRCm39)	I79V	probably damaging	Het
Cyp2j6	T	C	4: 96,412,014 (GRCm39)	D418G	probably benign	Het
Ddx28	G	C	8: 106,737,190 (GRCm39)	D289E	probably damaging	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fam135a	A	C	1: 24,068,887 (GRCm39)	S448A	probably benign	Het
Fbxo33	G	T	12: 59,251,626 (GRCm39)	N30K	possibly damaging	Het
Fgf12	A	C	16: 28,217,093 (GRCm39)	Y21D	probably damaging	Het
Galntl5	C	T	5: 25,415,432 (GRCm39)	S288L	probably benign	Het
Gart	G	T	16: 91,422,237 (GRCm39)	A760D	probably damaging	Het
Gba2	A	T	4: 43,573,869 (GRCm39)		probably benign	Het
Gm10518	C	A	1: 179,631,357 (GRCm39)	S139*	probably null	Het
Gm4781	A	T	10: 100,232,837 (GRCm39)		noncoding transcript	Het
Gm9790	A	G	3: 85,823,156 (GRCm39)		noncoding transcript	Het
Gmps	G	A	3: 63,893,075 (GRCm39)	G127R	probably damaging	Het
Golim4	A	T	3: 75,802,443 (GRCm39)	V283E	probably benign	Het
Gprc5a	A	T	6: 135,055,918 (GRCm39)	I122F	possibly damaging	Het
Gzmc	T	C	14: 56,471,341 (GRCm39)	K67E	probably benign	Het
Hapln3	C	A	7: 78,771,638 (GRCm39)	V84L	probably benign	Het
Hif3a	T	A	7: 16,778,789 (GRCm39)	N377Y	possibly damaging	Het
Ifi211	G	A	1: 173,726,969 (GRCm39)	H392Y	probably damaging	Het
Iqgap3	T	A	3: 88,015,663 (GRCm39)		probably benign	Het
Itga10	T	A	3: 96,559,141 (GRCm39)	F410Y	probably damaging	Het
Jup	T	C	11: 100,263,260 (GRCm39)	Y705C	probably damaging	Het
Khsrp	C	T	17: 57,332,597 (GRCm39)	A228T	probably benign	Het
Lmntd2	C	T	7: 140,790,998 (GRCm39)	G445D	probably damaging	Het
Lyst	T	C	13: 13,809,290 (GRCm39)	V320A	possibly damaging	Het
Magel2	T	A	7: 62,027,988 (GRCm39)	H297Q	possibly damaging	Het
Mbd6	T	C	10: 127,123,286 (GRCm39)	E33G	probably damaging	Het
Mob3b	A	G	4: 34,985,910 (GRCm39)		probably benign	Het
Mroh2a	T	C	1: 88,162,334 (GRCm39)		probably null	Het
Mroh2a	C	T	1: 88,158,402 (GRCm39)	R150*	probably null	Het
Mymk	A	T	2: 26,952,346 (GRCm39)	W174R	probably damaging	Het
Nckap1	C	T	2: 80,348,286 (GRCm39)	S889N	probably benign	Het
Nipal3	T	C	4: 135,174,599 (GRCm39)	Y384C	possibly damaging	Het
Nt5c3b	A	G	11: 100,330,920 (GRCm39)		probably benign	Het
Obox6	T	A	7: 15,567,750 (GRCm39)	L232F	probably damaging	Het
Obscn	A	C	11: 58,997,113 (GRCm39)		probably benign	Het
Or7g12	T	C	9: 18,899,839 (GRCm39)	L185P	probably damaging	Het
Phkb	A	G	8: 86,748,278 (GRCm39)	I706V	probably benign	Het
Plcb2	A	G	2: 118,546,168 (GRCm39)		probably benign	Het
Plek2	T	C	12: 78,941,184 (GRCm39)	D216G	probably damaging	Het
Plxnb2	T	C	15: 89,046,665 (GRCm39)	Y855C	probably damaging	Het
Prkcz	T	C	4: 155,355,713 (GRCm39)	T227A	probably damaging	Het
Psma7	A	T	2: 179,679,215 (GRCm39)	D184E	probably benign	Het
Rai14	T	A	15: 10,592,282 (GRCm39)	L204F	probably damaging	Het
Ralgapa2	A	G	2: 146,199,920 (GRCm39)	V1208A	probably benign	Het
Rapgef6	A	G	11: 54,582,458 (GRCm39)	R67G	possibly damaging	Het
Ryr2	C	T	13: 11,618,665 (GRCm39)		probably benign	Het
Satb1	T	C	17: 52,047,027 (GRCm39)	S763G	probably benign	Het
Sdk1	A	T	5: 142,020,292 (GRCm39)	H690L	probably benign	Het
Sfrp5	A	C	19: 42,190,143 (GRCm39)	V103G	possibly damaging	Het
Six6	A	G	12: 72,988,451 (GRCm39)	E208G	probably benign	Het
Sspo	A	T	6: 48,437,334 (GRCm39)	H1364L	probably benign	Het
Stard9	A	T	2: 120,529,973 (GRCm39)	T2077S	probably benign	Het
Tacr3	T	C	3: 134,535,254 (GRCm39)	L74P	probably damaging	Het
Tep1	T	C	14: 51,074,245 (GRCm39)	E1880G	probably benign	Het
Tgfb3	A	T	12: 86,116,517 (GRCm39)		probably benign	Het
Thap7	G	A	16: 17,346,576 (GRCm39)	P136S	probably damaging	Het
Thoc2l	T	A	5: 104,667,789 (GRCm39)	Y770*	probably null	Het
Tmc2	T	C	2: 130,098,036 (GRCm39)	V717A	possibly damaging	Het
Usp43	T	A	11: 67,778,593 (GRCm39)	S446C	probably damaging	Het
Vwa3a	C	T	7: 120,379,371 (GRCm39)	S492L	probably damaging	Het
Wdhd1	T	A	14: 47,493,672 (GRCm39)	N16I	probably damaging	Het
Wdr95	A	T	5: 149,516,566 (GRCm39)	D327V	probably damaging	Het
Zfp128	T	G	7: 12,624,563 (GRCm39)	Y310*	probably null	Het

Other mutations in Adgrg3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01096:Adgrg3	APN	8	95,766,221 (GRCm39)	missense	possibly damaging	0.90
IGL01724:Adgrg3	APN	8	95,766,053 (GRCm39)	missense	probably benign	0.04
IGL02111:Adgrg3	APN	8	95,761,627 (GRCm39)	missense	probably damaging	0.96
IGL02142:Adgrg3	APN	8	95,766,483 (GRCm39)	missense	probably damaging	1.00
IGL02577:Adgrg3	APN	8	95,766,555 (GRCm39)	missense	probably damaging	0.99
IGL02940:Adgrg3	APN	8	95,760,084 (GRCm39)	missense	possibly damaging	0.48
IGL03395:Adgrg3	APN	8	95,761,701 (GRCm39)	missense	probably damaging	1.00
R0111:Adgrg3	UTSW	8	95,761,738 (GRCm39)	splice site	probably benign
R0288:Adgrg3	UTSW	8	95,766,568 (GRCm39)	missense	possibly damaging	0.92
R0403:Adgrg3	UTSW	8	95,763,550 (GRCm39)	missense	probably benign	0.07
R1553:Adgrg3	UTSW	8	95,766,896 (GRCm39)	missense	possibly damaging	0.90
R1667:Adgrg3	UTSW	8	95,760,001 (GRCm39)	nonsense	probably null
R1872:Adgrg3	UTSW	8	95,760,070 (GRCm39)	missense	possibly damaging	0.87
R1882:Adgrg3	UTSW	8	95,766,943 (GRCm39)	missense	probably benign	0.03
R1924:Adgrg3	UTSW	8	95,762,562 (GRCm39)	missense	probably benign
R1998:Adgrg3	UTSW	8	95,763,296 (GRCm39)	missense	probably damaging	1.00
R2090:Adgrg3	UTSW	8	95,766,558 (GRCm39)	missense	possibly damaging	0.54
R2696:Adgrg3	UTSW	8	95,747,702 (GRCm39)	missense	probably benign	0.01
R3846:Adgrg3	UTSW	8	95,767,049 (GRCm39)	missense	probably benign	0.07
R4013:Adgrg3	UTSW	8	95,761,727 (GRCm39)	splice site	probably benign
R4405:Adgrg3	UTSW	8	95,763,536 (GRCm39)	missense	probably benign	0.15
R4622:Adgrg3	UTSW	8	95,767,153 (GRCm39)	missense	probably damaging	1.00
R4878:Adgrg3	UTSW	8	95,761,714 (GRCm39)	missense	possibly damaging	0.86
R5101:Adgrg3	UTSW	8	95,763,563 (GRCm39)	missense	probably benign	0.00
R5309:Adgrg3	UTSW	8	95,766,492 (GRCm39)	missense	probably benign	0.00
R5312:Adgrg3	UTSW	8	95,766,492 (GRCm39)	missense	probably benign	0.00
R5353:Adgrg3	UTSW	8	95,762,556 (GRCm39)	missense	probably damaging	0.99
R5820:Adgrg3	UTSW	8	95,766,221 (GRCm39)	missense	possibly damaging	0.90
R6240:Adgrg3	UTSW	8	95,766,544 (GRCm39)	missense	probably benign	0.23
R6272:Adgrg3	UTSW	8	95,762,889 (GRCm39)	missense	noncoding transcript
R7110:Adgrg3	UTSW	8	95,761,591 (GRCm39)	missense	possibly damaging	0.62
R7645:Adgrg3	UTSW	8	95,761,392 (GRCm39)	intron	probably benign
R8178:Adgrg3	UTSW	8	95,761,675 (GRCm39)	missense	probably damaging	0.98
R8397:Adgrg3	UTSW	8	95,767,141 (GRCm39)	missense	probably benign	0.01
R8730:Adgrg3	UTSW	8	95,766,556 (GRCm39)	missense	probably benign	0.09
R8951:Adgrg3	UTSW	8	95,761,362 (GRCm39)	intron	probably benign
R9100:Adgrg3	UTSW	8	95,762,891 (GRCm39)	intron	probably benign
R9523:Adgrg3	UTSW	8	95,766,186 (GRCm39)	missense	probably benign	0.06
R9583:Adgrg3	UTSW	8	95,760,071 (GRCm39)	missense	probably benign	0.00
R9589:Adgrg3	UTSW	8	95,760,093 (GRCm39)	missense	possibly damaging	0.87
X0017:Adgrg3	UTSW	8	95,744,398 (GRCm39)	start codon destroyed	probably null	0.53

Predicted Primers

PCR Primer
(F):5'- GGGTCAGTTCCCAAGTCGCTCTTC -3'
(R):5'- TCCCAGCATCCTGGCTGCTAAC -3'

Sequencing Primer
(F):5'- CTTTACCTTAAGAGCCCAGGGTC -3'
(R):5'- CTGACTGCAATGCTGCATAG -3'

Posted On

2014-05-14