Mutagenetix > Incidental Mutation

Incidental Mutation 'R1711:Osbpl9'

190535

Institutional Source

Beutler Lab

Gene Symbol

Osbpl9

Ensembl Gene

ENSMUSG00000028559

Gene Name

oxysterol binding protein-like 9

Synonyms

ORP-9, 2600011I06Rik

MMRRC Submission

039744-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1711 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

108918342-109059469 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 108923415 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 495 (C495S)

Ref Sequence

ENSEMBL: ENSMUSP00000124742 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030288] [ENSMUST00000065977] [ENSMUST00000084366] [ENSMUST00000102736] [ENSMUST00000162787] [ENSMUST00000194478] [ENSMUST00000160774] [ENSMUST00000161363] [ENSMUST00000160271] [ENSMUST00000159545]

AlphaFold

A2A8Z1

Predicted Effect

probably benign
Transcript: ENSMUST00000030288
AA Change: C512S

PolyPhen 2 Score 0.334 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000030288
Gene: ENSMUSG00000028559
AA Change: C512S

Domain	Start	End	E-Value	Type
PH	3	101	8.5e-17	SMART
low complexity region	253	274	N/A	INTRINSIC
low complexity region	285	301	N/A	INTRINSIC
low complexity region	349	362	N/A	INTRINSIC
Pfam:Oxysterol_BP	377	729	7.3e-79	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000065977

SMART Domains

Protein: ENSMUSP00000068328
Gene: ENSMUSG00000053510

Domain	Start	End	E-Value	Type
low complexity region	139	165	N/A	INTRINSIC
low complexity region	172	209	N/A	INTRINSIC
Pfam:Peptidase_M16	210	348	1.1e-43	PFAM
Pfam:Peptidase_M16_C	373	559	2.3e-22	PFAM
Pfam:Peptidase_M16_C	849	1032	1.5e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000084366
AA Change: C415S

PolyPhen 2 Score 0.406 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000081396
Gene: ENSMUSG00000028559
AA Change: C415S

Domain	Start	End	E-Value	Type
low complexity region	156	177	N/A	INTRINSIC
low complexity region	188	204	N/A	INTRINSIC
low complexity region	252	265	N/A	INTRINSIC
Pfam:Oxysterol_BP	277	634	7.2e-82	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000102736

SMART Domains

Protein: ENSMUSP00000099797
Gene: ENSMUSG00000053510

Domain	Start	End	E-Value	Type
low complexity region	139	165	N/A	INTRINSIC
low complexity region	172	209	N/A	INTRINSIC
Pfam:Peptidase_M16	210	348	1.4e-43	PFAM
Pfam:Peptidase_M16_C	400	515	1.1e-9	PFAM
Pfam:Peptidase_M16_C	805	988	2e-14	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126288

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139412

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150784

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162787
AA Change: C499S

PolyPhen 2 Score 0.947 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000124370
Gene: ENSMUSG00000028559
AA Change: C499S

Domain	Start	End	E-Value	Type
PH	3	101	8.5e-17	SMART
low complexity region	240	261	N/A	INTRINSIC
low complexity region	272	288	N/A	INTRINSIC
low complexity region	336	349	N/A	INTRINSIC
Pfam:Oxysterol_BP	361	718	2.8e-82	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000194478
AA Change: C522S

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000141991
Gene: ENSMUSG00000028559
AA Change: C522S

Domain	Start	End	E-Value	Type
PH	3	101	3.7e-19	SMART
low complexity region	263	284	N/A	INTRINSIC
low complexity region	295	311	N/A	INTRINSIC
low complexity region	359	372	N/A	INTRINSIC
Pfam:Oxysterol_BP	384	741	2e-79	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000160774
AA Change: C495S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124742
Gene: ENSMUSG00000028559
AA Change: C495S

Domain	Start	End	E-Value	Type
PH	3	84	6.46e-8	SMART
low complexity region	236	257	N/A	INTRINSIC
low complexity region	268	284	N/A	INTRINSIC
low complexity region	332	345	N/A	INTRINSIC
Pfam:Oxysterol_BP	357	714	2.8e-82	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000161363
AA Change: C432S

PolyPhen 2 Score 0.334 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000125714
Gene: ENSMUSG00000028559
AA Change: C432S

Domain	Start	End	E-Value	Type
Blast:PH	13	34	3e-6	BLAST
low complexity region	173	194	N/A	INTRINSIC
low complexity region	205	221	N/A	INTRINSIC
low complexity region	269	282	N/A	INTRINSIC
Pfam:Oxysterol_BP	294	651	2.2e-82	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000160271
AA Change: C402S

PolyPhen 2 Score 0.334 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000124112
Gene: ENSMUSG00000028559
AA Change: C402S

Domain	Start	End	E-Value	Type
low complexity region	143	164	N/A	INTRINSIC
low complexity region	175	191	N/A	INTRINSIC
low complexity region	239	252	N/A	INTRINSIC
Pfam:Oxysterol_BP	264	621	4.7e-82	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155434

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159198

Predicted Effect

probably benign
Transcript: ENSMUST00000159545

SMART Domains

Protein: ENSMUSP00000123856
Gene: ENSMUSG00000028559

Domain	Start	End	E-Value	Type
Blast:PH	3	54	6e-33	BLAST
SCOP:d1pls__	4	46	9e-8	SMART
PDB:2KCJ\|A	4	55	1e-10	PDB

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.6%
20x: 93.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010]

Allele List at MGI

Other mutations in this stock

Total: 111 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	C	T	13: 77,418,039 (GRCm39)	T741I	possibly damaging	Het
Aadacl4fm2	A	T	4: 144,281,976 (GRCm39)	I272K	probably damaging	Het
Acot3	A	T	12: 84,100,347 (GRCm39)	Q41L	probably damaging	Het
Actl7b	G	A	4: 56,740,165 (GRCm39)	Q398*	probably null	Het
Adgrb2	C	A	4: 129,886,417 (GRCm39)	Q186K	probably damaging	Het
Akr1a1	A	G	4: 116,495,171 (GRCm39)		probably null	Het
Ap3s2	A	T	7: 79,530,238 (GRCm39)	F192L	probably damaging	Het
Apobr	T	G	7: 126,184,151 (GRCm39)		probably null	Het
Arhgap5	A	G	12: 52,566,128 (GRCm39)	N1033S	probably damaging	Het
Arid3c	G	T	4: 41,725,947 (GRCm39)	R219S	probably damaging	Het
Bves	C	T	10: 45,223,961 (GRCm39)	T207M	probably damaging	Het
Cacna1d	T	C	14: 29,788,013 (GRCm39)	K1619R	probably damaging	Het
Caps2	A	T	10: 112,026,883 (GRCm39)	D223V	possibly damaging	Het
Cd3g	A	C	9: 44,885,640 (GRCm39)	L35R	probably damaging	Het
Cdh23	A	T	10: 60,359,315 (GRCm39)	V261E	probably benign	Het
Cfap54	T	G	10: 92,846,882 (GRCm39)	T1028P	probably damaging	Het
Ch25h	C	A	19: 34,451,686 (GRCm39)	V281L	probably benign	Het
Cip2a	A	C	16: 48,837,849 (GRCm39)	I850L	probably benign	Het
Clu	G	T	14: 66,218,354 (GRCm39)	V405L	possibly damaging	Het
Col6a3	A	T	1: 90,757,935 (GRCm39)	H6Q	probably damaging	Het
Cps1	G	A	1: 67,207,533 (GRCm39)		probably null	Het
Ctr9	T	C	7: 110,654,870 (GRCm39)	S1134P	unknown	Het
Cts7	C	T	13: 61,500,624 (GRCm39)	G308S	probably damaging	Het
Cyp2c39	C	A	19: 39,555,335 (GRCm39)	T385K	probably damaging	Het
Dennd5a	A	T	7: 109,517,919 (GRCm39)	D596E	probably benign	Het
Disc1	T	A	8: 125,851,349 (GRCm39)	I413K	probably benign	Het
Dll3	C	T	7: 27,993,922 (GRCm39)	G505D	probably damaging	Het
Dnah3	A	G	7: 119,677,794 (GRCm39)	W377R	probably damaging	Het
Dop1b	A	G	16: 93,596,814 (GRCm39)	D1792G	probably damaging	Het
Dpep3	T	A	8: 106,700,325 (GRCm39)	R460S	probably benign	Het
Ebf4	A	G	2: 130,200,751 (GRCm39)	N302S	probably damaging	Het
Efcab3	A	G	11: 104,611,514 (GRCm39)	K452R	probably benign	Het
Egflam	T	C	15: 7,319,396 (GRCm39)	E194G	possibly damaging	Het
Ep400	A	T	5: 110,841,174 (GRCm39)		probably benign	Het
Ercc6	T	C	14: 32,248,133 (GRCm39)	M228T	probably damaging	Het
Fbxo9	T	C	9: 77,994,529 (GRCm39)	T264A	probably damaging	Het
Fcrl5	C	A	3: 87,364,721 (GRCm39)	P486T	possibly damaging	Het
Fyb1	T	C	15: 6,609,960 (GRCm39)	F178L	probably damaging	Het
Gaa	T	A	11: 119,171,286 (GRCm39)	I646N	probably damaging	Het
Gm5431	A	T	11: 48,785,853 (GRCm39)	V174E	possibly damaging	Het
Gnpat	T	G	8: 125,613,691 (GRCm39)		probably null	Het
Gucy1a2	G	T	9: 3,759,622 (GRCm39)	R476I	probably benign	Het
Hars1	A	G	18: 36,904,156 (GRCm39)	L241P	probably damaging	Het
Haus5	G	A	7: 30,357,328 (GRCm39)	Q399*	probably null	Het
Hephl1	T	C	9: 14,970,542 (GRCm39)	E984G	probably damaging	Het
Hmces	C	T	6: 87,898,574 (GRCm39)	Q132*	probably null	Het
Hsp90b1	G	T	10: 86,530,389 (GRCm39)	T490K	probably damaging	Het
Idh2	T	G	7: 79,748,906 (GRCm39)	E125A	probably damaging	Het
Ipo13	G	T	4: 117,761,719 (GRCm39)	H465Q	probably benign	Het
Isca2	C	A	12: 84,820,393 (GRCm39)	T31K	probably damaging	Het
Itga9	T	C	9: 118,527,529 (GRCm39)	V560A	probably benign	Het
Jhy	G	A	9: 40,822,453 (GRCm39)	Q562*	probably null	Het
Kcnj5	A	G	9: 32,233,865 (GRCm39)	I150T	probably damaging	Het
Kmt2a	A	G	9: 44,752,918 (GRCm39)	I1419T	unknown	Het
Lix1	T	C	17: 17,666,320 (GRCm39)	F160L	possibly damaging	Het
Lonrf2	G	A	1: 38,852,357 (GRCm39)	P165S	probably benign	Het
Map4k1	A	T	7: 28,688,777 (GRCm39)	Q276L	possibly damaging	Het
Mmp9	G	A	2: 164,791,342 (GRCm39)	G171S	probably damaging	Het
Mvp	C	A	7: 126,594,907 (GRCm39)		probably null	Het
Mylk3	T	A	8: 86,091,460 (GRCm39)	E115V	probably damaging	Het
Nebl	T	A	2: 17,393,565 (GRCm39)	T603S	probably damaging	Het
Nudt15	T	C	14: 73,760,776 (GRCm39)	D105G	probably benign	Het
Or12j2	A	C	7: 139,915,912 (GRCm39)	T46P	possibly damaging	Het
Or2w1b	G	A	13: 21,300,476 (GRCm39)	V205I	probably benign	Het
Or51b6	T	A	7: 103,555,906 (GRCm39)	W87R	probably damaging	Het
Or52i2	G	T	7: 102,319,369 (GRCm39)	V81L	probably damaging	Het
Or52l1	A	T	7: 104,829,967 (GRCm39)	H199Q	probably damaging	Het
Or8g2b	A	G	9: 39,751,581 (GRCm39)	M284V	probably benign	Het
Pamr1	A	G	2: 102,471,197 (GRCm39)	T507A	probably benign	Het
Pcdhb9	T	A	18: 37,536,380 (GRCm39)	C791*	probably null	Het
Pcgf6	T	C	19: 47,038,957 (GRCm39)	E101G	probably damaging	Het
Pdgfrl	A	T	8: 41,438,831 (GRCm39)	I256F	probably benign	Het
Pdzd7	T	C	19: 45,033,950 (GRCm39)	R45G	possibly damaging	Het
Pecr	C	T	1: 72,316,568 (GRCm39)	V46I	possibly damaging	Het
Pgr	A	G	9: 8,922,715 (GRCm39)		probably null	Het
Pik3c2a	A	T	7: 116,017,162 (GRCm39)	Y198*	probably null	Het
Pp2d1	T	A	17: 53,822,338 (GRCm39)	M243L	possibly damaging	Het
Ppp1r1a	T	A	15: 103,441,919 (GRCm39)	I50L	possibly damaging	Het
Proc	T	C	18: 32,260,459 (GRCm39)	D222G	probably benign	Het
Ptprq	T	A	10: 107,370,560 (GRCm39)	R2044*	probably null	Het
Ranbp2	T	C	10: 58,296,341 (GRCm39)	V326A	probably benign	Het
Reep6	T	A	10: 80,169,815 (GRCm39)	F122I	possibly damaging	Het
Rubcn	C	A	16: 32,663,471 (GRCm39)	R388S	probably damaging	Het
Rusc1	A	G	3: 88,996,600 (GRCm39)	L62P	probably damaging	Het
Satb2	T	C	1: 56,889,448 (GRCm39)	N423S	probably damaging	Het
Scart1	G	T	7: 139,800,522 (GRCm39)	C101F	probably damaging	Het
Serpinb9d	A	G	13: 33,384,731 (GRCm39)	K236R	probably benign	Het
Slc4a7	A	G	14: 14,765,709 (GRCm38)	R680G	probably benign	Het
Slitrk1	T	A	14: 109,150,528 (GRCm39)	Y61F	probably benign	Het
Son	A	G	16: 91,457,114 (GRCm39)		probably benign	Het
Sparc	T	A	11: 55,286,602 (GRCm39)		probably null	Het
Spon2	A	G	5: 33,373,729 (GRCm39)	F194S	probably damaging	Het
Spta1	A	G	1: 174,068,608 (GRCm39)	E2136G	probably damaging	Het
Stat4	T	C	1: 52,146,084 (GRCm39)	S746P	probably damaging	Het
Stk31	T	A	6: 49,446,238 (GRCm39)	S958R	probably benign	Het
Stom	T	A	2: 35,205,929 (GRCm39)	I267F	probably damaging	Het
Stx12	A	T	4: 132,585,788 (GRCm39)	D197E	probably damaging	Het
Taok3	A	T	5: 117,393,991 (GRCm39)	N588I	possibly damaging	Het
Tgs1	A	G	4: 3,598,658 (GRCm39)	D657G	probably damaging	Het
Trim6	A	G	7: 103,882,044 (GRCm39)	T432A	probably damaging	Het
Trim72	C	G	7: 127,603,757 (GRCm39)	C34W	probably damaging	Het
Trpc4ap	A	T	2: 155,499,664 (GRCm39)	I286N	probably benign	Het
Ttn	A	G	2: 76,693,905 (GRCm39)	V321A	possibly damaging	Het
Utp4	T	A	8: 107,645,352 (GRCm39)	I583N	probably damaging	Het
Wdfy2	G	T	14: 63,181,546 (GRCm39)	M225I	probably benign	Het
Wfdc1	A	G	8: 120,407,776 (GRCm39)	T134A	probably benign	Het
Wnt9b	A	G	11: 103,622,954 (GRCm39)	S150P	probably damaging	Het
Zbtb46	A	T	2: 181,053,477 (GRCm39)	F412I	probably damaging	Het
Zc3h15	G	A	2: 83,491,492 (GRCm39)	R240H	probably benign	Het
Zfp11	A	T	5: 129,733,737 (GRCm39)	Y575N	probably benign	Het
Zfp687	T	C	3: 94,919,200 (GRCm39)	M191V	probably benign	Het

Other mutations in Osbpl9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00722:Osbpl9	APN	4	108,929,207 (GRCm39)	missense	probably damaging	1.00
IGL00793:Osbpl9	APN	4	108,944,628 (GRCm39)	missense	probably damaging	0.99
IGL00809:Osbpl9	APN	4	108,990,960 (GRCm39)	missense	probably damaging	1.00
IGL02071:Osbpl9	APN	4	108,929,176 (GRCm39)	missense	probably damaging	1.00
IGL02547:Osbpl9	APN	4	108,925,680 (GRCm39)	nonsense	probably null
IGL02822:Osbpl9	APN	4	108,930,118 (GRCm39)	missense	probably damaging	1.00
IGL03074:Osbpl9	APN	4	108,929,158 (GRCm39)	missense	probably damaging	1.00
IGL03193:Osbpl9	APN	4	108,924,163 (GRCm39)	missense	possibly damaging	0.90
IGL03196:Osbpl9	APN	4	108,930,061 (GRCm39)	missense	probably damaging	1.00
IGL03306:Osbpl9	APN	4	109,029,529 (GRCm39)	splice site	probably benign
IGL03323:Osbpl9	APN	4	108,919,656 (GRCm39)	splice site	probably benign
Oblong	UTSW	4	108,948,876 (GRCm39)	missense	possibly damaging	0.62
R0211:Osbpl9	UTSW	4	108,930,321 (GRCm39)	missense	probably damaging	1.00
R0368:Osbpl9	UTSW	4	108,924,129 (GRCm39)	missense	probably damaging	1.00
R0620:Osbpl9	UTSW	4	108,940,325 (GRCm39)	missense	probably damaging	1.00
R1439:Osbpl9	UTSW	4	108,958,353 (GRCm39)	missense	probably damaging	1.00
R1757:Osbpl9	UTSW	4	108,921,780 (GRCm39)	missense	probably damaging	1.00
R2237:Osbpl9	UTSW	4	109,013,854 (GRCm39)	missense	probably damaging	1.00
R2295:Osbpl9	UTSW	4	109,059,331 (GRCm39)	missense	probably damaging	0.99
R2418:Osbpl9	UTSW	4	108,923,415 (GRCm39)	missense	probably damaging	1.00
R3111:Osbpl9	UTSW	4	108,940,290 (GRCm39)	missense	probably benign	0.08
R4202:Osbpl9	UTSW	4	109,029,437 (GRCm39)	intron	probably benign
R4672:Osbpl9	UTSW	4	108,921,806 (GRCm39)	missense	possibly damaging	0.82
R4706:Osbpl9	UTSW	4	109,013,884 (GRCm39)	missense	probably damaging	1.00
R4856:Osbpl9	UTSW	4	108,925,564 (GRCm39)	missense	probably benign	0.38
R4886:Osbpl9	UTSW	4	108,925,564 (GRCm39)	missense	probably benign	0.38
R5035:Osbpl9	UTSW	4	108,923,364 (GRCm39)	missense	probably damaging	0.99
R5309:Osbpl9	UTSW	4	108,923,352 (GRCm39)	missense	probably damaging	1.00
R5400:Osbpl9	UTSW	4	108,919,497 (GRCm39)	nonsense	probably null
R5719:Osbpl9	UTSW	4	108,919,763 (GRCm39)	nonsense	probably null
R5810:Osbpl9	UTSW	4	108,943,571 (GRCm39)	missense	probably benign	0.00
R6237:Osbpl9	UTSW	4	109,013,899 (GRCm39)	missense	probably damaging	1.00
R6575:Osbpl9	UTSW	4	108,930,129 (GRCm39)	missense	possibly damaging	0.89
R6648:Osbpl9	UTSW	4	108,948,876 (GRCm39)	missense	possibly damaging	0.62
R6675:Osbpl9	UTSW	4	108,991,025 (GRCm39)	splice site	probably null
R7130:Osbpl9	UTSW	4	108,940,296 (GRCm39)	missense	probably benign
R7356:Osbpl9	UTSW	4	108,925,677 (GRCm39)	nonsense	probably null
R7615:Osbpl9	UTSW	4	108,943,536 (GRCm39)	missense	probably damaging	1.00
R7753:Osbpl9	UTSW	4	108,990,970 (GRCm39)	missense	possibly damaging	0.86
R7772:Osbpl9	UTSW	4	108,923,384 (GRCm39)	missense	probably damaging	0.99
R7788:Osbpl9	UTSW	4	108,919,691 (GRCm39)	missense	probably benign	0.41
R8083:Osbpl9	UTSW	4	108,943,572 (GRCm39)	missense	possibly damaging	0.74
R8143:Osbpl9	UTSW	4	108,922,906 (GRCm39)	missense	probably benign	0.12
R8323:Osbpl9	UTSW	4	108,965,119 (GRCm39)	missense	probably benign	0.01
R8331:Osbpl9	UTSW	4	108,923,378 (GRCm39)	missense	probably damaging	1.00
R8406:Osbpl9	UTSW	4	108,921,770 (GRCm39)	missense	possibly damaging	0.82
R8531:Osbpl9	UTSW	4	109,013,908 (GRCm39)	missense	probably damaging	1.00
R8715:Osbpl9	UTSW	4	108,959,773 (GRCm39)	missense	probably benign	0.21
R8888:Osbpl9	UTSW	4	108,930,333 (GRCm39)	missense	probably benign	0.03
R8895:Osbpl9	UTSW	4	108,930,333 (GRCm39)	missense	probably benign	0.03
R9079:Osbpl9	UTSW	4	108,920,644 (GRCm39)	missense	possibly damaging	0.48
R9379:Osbpl9	UTSW	4	108,940,399 (GRCm39)	missense	probably damaging	0.99
R9775:Osbpl9	UTSW	4	109,013,747 (GRCm39)	intron	probably benign
Z1177:Osbpl9	UTSW	4	108,965,077 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- TGCCTTCCATAGCCATTGGGGAAC -3'
(R):5'- AGAACAGGACACGTCTTAAACCTGC -3'

Sequencing Primer
(F):5'- GGTGATATAGTTTATACCCCACCCAG -3'
(R):5'- TGCGGGAGTTGCCATAGC -3'

Posted On

2014-05-14