Mutagenetix > Incidental Mutation

Incidental Mutation 'R1778:Nfat5'

197246

Institutional Source

Beutler Lab

Gene Symbol

Nfat5

Ensembl Gene

ENSMUSG00000003847

Gene Name

nuclear factor of activated T cells 5

Synonyms

OREBP, B130038B15Rik, nfatz, TonEBP

MMRRC Submission

039809-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.910) question?

Stock #

R1778 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

108020102-108106149 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 108088421 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 579 (P579L)

Ref Sequence

ENSEMBL: ENSMUSP00000127784 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075922] [ENSMUST00000077440] [ENSMUST00000125721] [ENSMUST00000133026] [ENSMUST00000144100] [ENSMUST00000151114] [ENSMUST00000147588] [ENSMUST00000169453] [ENSMUST00000154474]

AlphaFold

Q9WV30

Predicted Effect

probably damaging
Transcript: ENSMUST00000075922
AA Change: P561L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000075311
Gene: ENSMUSG00000003847
AA Change: P561L

Domain	Start	End	E-Value	Type
low complexity region	52	98	N/A	INTRINSIC
low complexity region	179	192	N/A	INTRINSIC
Pfam:RHD	282	439	7.8e-23	PFAM
IPT	444	542	3.33e-15	SMART
low complexity region	647	653	N/A	INTRINSIC
low complexity region	734	754	N/A	INTRINSIC
low complexity region	793	810	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	915	920	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000077440
AA Change: P485L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000076653
Gene: ENSMUSG00000003847
AA Change: P485L

Domain	Start	End	E-Value	Type
low complexity region	6	22	N/A	INTRINSIC
low complexity region	103	116	N/A	INTRINSIC
Pfam:RHD	206	363	1.5e-22	PFAM
IPT	368	466	3.33e-15	SMART
low complexity region	571	577	N/A	INTRINSIC
low complexity region	658	678	N/A	INTRINSIC
low complexity region	717	734	N/A	INTRINSIC
low complexity region	822	834	N/A	INTRINSIC
low complexity region	839	844	N/A	INTRINSIC
low complexity region	887	901	N/A	INTRINSIC
internal_repeat_2	927	1110	7.13e-8	PROSPERO
internal_repeat_1	935	1128	2.59e-11	PROSPERO
internal_repeat_2	1122	1324	7.13e-8	PROSPERO
internal_repeat_1	1207	1426	2.59e-11	PROSPERO

Predicted Effect

probably damaging
Transcript: ENSMUST00000125721
AA Change: P561L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000116094
Gene: ENSMUSG00000003847
AA Change: P561L

Domain	Start	End	E-Value	Type
low complexity region	52	98	N/A	INTRINSIC
low complexity region	179	192	N/A	INTRINSIC
Pfam:RHD	282	439	1e-22	PFAM
IPT	444	542	3.33e-15	SMART
low complexity region	647	653	N/A	INTRINSIC
low complexity region	734	754	N/A	INTRINSIC
low complexity region	793	810	N/A	INTRINSIC
low complexity region	898	910	N/A	INTRINSIC
low complexity region	915	920	N/A	INTRINSIC
low complexity region	963	977	N/A	INTRINSIC
internal_repeat_2	1003	1186	2.22e-8	PROSPERO
internal_repeat_1	1011	1204	5.31e-12	PROSPERO
internal_repeat_2	1198	1400	2.22e-8	PROSPERO
internal_repeat_1	1283	1502	5.31e-12	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000126333

SMART Domains

Protein: ENSMUSP00000118130
Gene: ENSMUSG00000003847

Domain	Start	End	E-Value	Type
Pfam:RHD_DNA_bind	30	132	6.8e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126397

Predicted Effect

probably benign
Transcript: ENSMUST00000133026

SMART Domains

Protein: ENSMUSP00000116631
Gene: ENSMUSG00000003847

Domain	Start	End	E-Value	Type
low complexity region	70	88	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000144100

Predicted Effect

probably damaging
Transcript: ENSMUST00000151114
AA Change: P579L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000119370
Gene: ENSMUSG00000003847
AA Change: P579L

Domain	Start	End	E-Value	Type
low complexity region	70	116	N/A	INTRINSIC
low complexity region	197	210	N/A	INTRINSIC
Pfam:RHD_DNA_bind	300	457	1.1e-22	PFAM
IPT	462	560	3.33e-15	SMART
low complexity region	665	671	N/A	INTRINSIC
low complexity region	752	772	N/A	INTRINSIC
low complexity region	811	828	N/A	INTRINSIC
low complexity region	916	928	N/A	INTRINSIC
low complexity region	933	938	N/A	INTRINSIC
low complexity region	981	995	N/A	INTRINSIC
internal_repeat_2	1021	1204	2.24e-8	PROSPERO
internal_repeat_1	1029	1222	5.32e-12	PROSPERO
internal_repeat_2	1216	1418	2.24e-8	PROSPERO
internal_repeat_1	1301	1520	5.32e-12	PROSPERO

Predicted Effect

probably damaging
Transcript: ENSMUST00000147588
AA Change: P60L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000122871
Gene: ENSMUSG00000003847
AA Change: P60L

Domain	Start	End	E-Value	Type
Blast:IPT	1	42	3e-20	BLAST
PDB:1IMH\|D	1	44	2e-20	PDB
SCOP:d1bfta_	1	44	3e-14	SMART
low complexity region	146	152	N/A	INTRINSIC
low complexity region	233	253	N/A	INTRINSIC
low complexity region	292	309	N/A	INTRINSIC
low complexity region	397	409	N/A	INTRINSIC
low complexity region	414	419	N/A	INTRINSIC
low complexity region	462	476	N/A	INTRINSIC
internal_repeat_2	502	685	1.17e-6	PROSPERO
internal_repeat_1	510	703	1.39e-9	PROSPERO
internal_repeat_2	697	899	1.17e-6	PROSPERO
internal_repeat_1	782	1001	1.39e-9	PROSPERO

Predicted Effect

probably damaging
Transcript: ENSMUST00000169453
AA Change: P579L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127784
Gene: ENSMUSG00000003847
AA Change: P579L

Domain	Start	End	E-Value	Type
low complexity region	70	116	N/A	INTRINSIC
low complexity region	197	210	N/A	INTRINSIC
Pfam:RHD_DNA_bind	300	457	1.1e-22	PFAM
IPT	462	560	3.33e-15	SMART
low complexity region	665	671	N/A	INTRINSIC
low complexity region	752	772	N/A	INTRINSIC
low complexity region	811	828	N/A	INTRINSIC
low complexity region	916	928	N/A	INTRINSIC
low complexity region	933	938	N/A	INTRINSIC
low complexity region	981	995	N/A	INTRINSIC
internal_repeat_2	1021	1204	2.24e-8	PROSPERO
internal_repeat_1	1029	1222	5.32e-12	PROSPERO
internal_repeat_2	1216	1418	2.24e-8	PROSPERO
internal_repeat_1	1301	1520	5.32e-12	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000154474

SMART Domains

Protein: ENSMUSP00000115036
Gene: ENSMUSG00000003847

Domain	Start	End	E-Value	Type
low complexity region	52	70	N/A	INTRINSIC

Meta Mutation Damage Score

0.1099

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.6%
20x: 93.3%

Validation Efficiency

98% (107/109)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for one of several knock-out allele exhibit lethality between E14.5 and E17.5 as well as around P10 with kidney, cardiac or immune defects depending on the allele. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	T	C	7: 29,260,131 (GRCm39)		noncoding transcript	Het
4930568D16Rik	A	G	2: 35,244,995 (GRCm39)	M119T	probably damaging	Het
Aadacl2	A	G	3: 59,924,871 (GRCm39)		probably null	Het
Abca9	C	T	11: 110,021,542 (GRCm39)	W1056*	probably null	Het
Adam20	A	C	8: 41,249,698 (GRCm39)	T603P	possibly damaging	Het
Adgrl1	T	C	8: 84,656,666 (GRCm39)	L323P	probably damaging	Het
Afap1l2	C	T	19: 56,904,638 (GRCm39)	E628K	possibly damaging	Het
Ahctf1	A	T	1: 179,580,580 (GRCm39)	L1874Q	possibly damaging	Het
Ak8	A	G	2: 28,602,333 (GRCm39)	E89G	probably benign	Het
Aldh16a1	G	A	7: 44,796,732 (GRCm39)	R256C	probably damaging	Het
Anxa5	G	A	3: 36,519,480 (GRCm39)	T3M	probably damaging	Het
Arhgef16	T	C	4: 154,372,443 (GRCm39)	K210E	probably benign	Het
Baz2b	T	C	2: 59,836,480 (GRCm39)	T18A	unknown	Het
Bub1	A	C	2: 127,645,042 (GRCm39)	I960M	possibly damaging	Het
Cbln2	G	T	18: 86,731,272 (GRCm39)	D27Y	probably benign	Het
Ces2h	C	A	8: 105,741,239 (GRCm39)	P77Q	possibly damaging	Het
Cfap410	G	T	10: 77,818,778 (GRCm39)	A150S	probably benign	Het
Chga	T	A	12: 102,527,959 (GRCm39)	M150K	probably benign	Het
Chmp3	A	G	6: 71,554,791 (GRCm39)	E162G	probably benign	Het
Clip3	A	G	7: 29,996,861 (GRCm39)	N161S	probably damaging	Het
Col12a1	T	A	9: 79,511,867 (GRCm39)		probably benign	Het
Cuedc2	C	T	19: 46,320,079 (GRCm39)	G105D	probably benign	Het
Cyp2j7	A	C	4: 96,087,627 (GRCm39)	F428V	probably damaging	Het
Ddx60	A	G	8: 62,427,210 (GRCm39)	I762V	possibly damaging	Het
Def6	G	A	17: 28,439,160 (GRCm39)	R257Q	probably benign	Het
Dkkl1	A	T	7: 44,860,819 (GRCm39)		probably null	Het
Dnah3	A	G	7: 119,677,625 (GRCm39)	L433P	probably damaging	Het
Eif2s3y	C	T	Y: 1,011,287 (GRCm39)	R33C	probably benign	Het
Elavl2	T	A	4: 91,141,715 (GRCm39)	Y269F	probably damaging	Het
Esco2	A	G	14: 66,068,711 (GRCm39)	S200P	possibly damaging	Het
Fcrl1	T	A	3: 87,292,626 (GRCm39)		probably benign	Het
Fhod1	C	A	8: 106,056,309 (GRCm39)	D1135Y	probably damaging	Het
Fnbp1l	T	C	3: 122,383,796 (GRCm39)	N41D	possibly damaging	Het
Folh1	A	T	7: 86,410,907 (GRCm39)		probably null	Het
Fpr-rs7	T	C	17: 20,334,277 (GRCm39)	D71G	probably damaging	Het
Gm10271	A	G	10: 116,797,844 (GRCm39)		probably benign	Het
Gm4841	A	T	18: 60,404,020 (GRCm39)	Y24*	probably null	Het
Greb1	G	A	12: 16,740,895 (GRCm39)	R1396C	probably benign	Het
Hectd1	C	T	12: 51,800,590 (GRCm39)	C2076Y	probably damaging	Het
Hnrnpu	A	G	1: 178,152,806 (GRCm39)		probably benign	Het
Ice2	T	C	9: 69,322,930 (GRCm39)	I475T	probably benign	Het
Ifit1bl1	T	A	19: 34,571,593 (GRCm39)	Q288L	probably damaging	Het
Ift56	T	A	6: 38,386,411 (GRCm39)	D377E	possibly damaging	Het
Ik	T	C	18: 36,889,871 (GRCm39)		probably benign	Het
Kidins220	C	T	12: 25,063,445 (GRCm39)		probably benign	Het
Kmt2c	T	C	5: 25,577,972 (GRCm39)	D768G	probably benign	Het
Kmt2e	A	G	5: 23,697,362 (GRCm39)	T87A	probably damaging	Het
Lama5	G	A	2: 179,837,274 (GRCm39)		probably benign	Het
Lmbr1l	A	G	15: 98,810,357 (GRCm39)	S85P	probably damaging	Het
Lrig2	A	G	3: 104,374,682 (GRCm39)		probably benign	Het
Lrig3	A	G	10: 125,845,944 (GRCm39)	D791G	probably damaging	Het
Lrrc2	G	A	9: 110,809,908 (GRCm39)	V315M	probably benign	Het
Lrrc4b	A	G	7: 44,111,823 (GRCm39)	D565G	probably benign	Het
Mpped1	C	T	15: 83,676,191 (GRCm39)		probably benign	Het
Msrb2	T	A	2: 19,388,114 (GRCm39)	D87E	probably benign	Het
Muc20	T	C	16: 32,614,511 (GRCm39)	T289A	possibly damaging	Het
Myo15a	C	A	11: 60,369,238 (GRCm39)	P666Q	possibly damaging	Het
Nipbl	A	C	15: 8,348,972 (GRCm39)	M1920R	probably damaging	Het
Nuak1	T	C	10: 84,210,738 (GRCm39)		probably null	Het
Nup210l	A	G	3: 90,096,793 (GRCm39)	E1334G	probably damaging	Het
Odad2	A	T	18: 7,127,388 (GRCm39)	C942S	probably damaging	Het
Or52e8b	A	T	7: 104,673,370 (GRCm39)	N272K	probably benign	Het
Or5an1c	T	A	19: 12,218,819 (GRCm39)	I69F	probably benign	Het
Or6c6c	T	C	10: 129,541,574 (GRCm39)	Y276H	probably benign	Het
Or9s13	G	A	1: 92,548,342 (GRCm39)	G238E	possibly damaging	Het
Or9s14	A	T	1: 92,535,831 (GRCm39)	N91Y	possibly damaging	Het
P4ha3	A	T	7: 99,949,898 (GRCm39)		probably null	Het
Pbld2	C	T	10: 62,890,150 (GRCm39)	A186V	probably benign	Het
Pcdh18	A	T	3: 49,710,083 (GRCm39)	Y411N	probably benign	Het
Pgf	A	G	12: 85,218,541 (GRCm39)	S70P	probably benign	Het
Phrf1	T	A	7: 140,812,369 (GRCm39)	D44E	probably benign	Het
Pla2g4a	A	G	1: 149,778,196 (GRCm39)		probably benign	Het
Plce1	A	G	19: 38,769,234 (GRCm39)		probably benign	Het
Plxna2	A	G	1: 194,493,278 (GRCm39)	N1851S	probably benign	Het
Prl7a2	G	A	13: 27,843,254 (GRCm39)	T183I	probably damaging	Het
Prxl2b	A	T	4: 154,981,814 (GRCm39)	V151D	probably damaging	Het
Ptger4	A	T	15: 5,264,576 (GRCm39)	L335H	probably damaging	Het
Ptgr1	A	G	4: 58,984,865 (GRCm39)		probably benign	Het
Rdh7	A	G	10: 127,720,590 (GRCm39)	S261P	probably benign	Het
Reg3a	A	G	6: 78,360,269 (GRCm39)	T150A	probably benign	Het
Rnf111	A	G	9: 70,383,394 (GRCm39)	S180P	probably benign	Het
Rnf17	A	G	14: 56,759,856 (GRCm39)	M1554V	probably damaging	Het
Rnf215	T	A	11: 4,085,873 (GRCm39)	Y117*	probably null	Het
Rnps1-ps	A	T	6: 7,983,124 (GRCm39)		noncoding transcript	Het
Rogdi	T	C	16: 4,828,369 (GRCm39)	T165A	probably benign	Het
Rpe65	A	G	3: 159,328,485 (GRCm39)	Y431C	probably damaging	Het
Scube1	A	G	15: 83,494,405 (GRCm39)	F844S	probably damaging	Het
Sdr16c6	T	A	4: 4,058,814 (GRCm39)	E257D	probably benign	Het
Sim1	C	A	10: 50,857,649 (GRCm39)	C466*	probably null	Het
Spi1	T	A	2: 90,929,867 (GRCm39)	H46Q	probably damaging	Het
Sumf2	C	A	5: 129,873,909 (GRCm39)		probably benign	Het
Tbcb	A	G	7: 29,931,037 (GRCm39)	Y28H	probably benign	Het
Tecta	C	T	9: 42,254,927 (GRCm39)	C1752Y	probably damaging	Het
Tep1	T	C	14: 51,067,079 (GRCm39)		probably benign	Het
Tmem245	A	T	4: 56,903,968 (GRCm39)	W591R	probably damaging	Het
Tnxb	A	G	17: 34,902,548 (GRCm39)	I1134V	probably benign	Het
Trappc14	T	C	5: 138,260,739 (GRCm39)		probably null	Het
Trim13	G	T	14: 61,843,068 (GRCm39)	A362S	probably benign	Het
Trim47	T	A	11: 116,000,646 (GRCm39)	D134V	probably damaging	Het
Utrn	C	A	10: 12,312,108 (GRCm39)	D616Y	probably damaging	Het
Vnn1	G	T	10: 23,775,415 (GRCm39)	A222S	possibly damaging	Het
Wdr17	A	G	8: 55,143,249 (GRCm39)	W110R	probably damaging	Het
Wwp1	G	T	4: 19,627,892 (GRCm39)	Y700*	probably null	Het
Zbtb21	T	C	16: 97,751,785 (GRCm39)	S661G	probably benign	Het
Zfp955b	A	G	17: 33,521,788 (GRCm39)	K419R	probably benign	Het

Other mutations in Nfat5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Nfat5	APN	8	108,094,146 (GRCm39)	missense	probably damaging	1.00
IGL01145:Nfat5	APN	8	108,093,847 (GRCm39)	missense	probably damaging	0.99
IGL01700:Nfat5	APN	8	108,065,762 (GRCm39)	missense	probably damaging	0.99
IGL01721:Nfat5	APN	8	108,071,611 (GRCm39)	critical splice donor site	probably null
IGL01796:Nfat5	APN	8	108,094,273 (GRCm39)	missense	probably damaging	1.00
IGL01976:Nfat5	APN	8	108,094,191 (GRCm39)	missense	probably damaging	1.00
IGL02063:Nfat5	APN	8	108,088,450 (GRCm39)	missense	probably benign	0.03
IGL02150:Nfat5	APN	8	108,094,584 (GRCm39)	nonsense	probably null
IGL02174:Nfat5	APN	8	108,065,683 (GRCm39)	missense	probably damaging	1.00
IGL02224:Nfat5	APN	8	108,071,447 (GRCm39)	missense	probably benign	0.00
IGL02226:Nfat5	APN	8	108,078,154 (GRCm39)	nonsense	probably null
IGL02324:Nfat5	APN	8	108,092,808 (GRCm39)	splice site	probably benign
IGL02724:Nfat5	APN	8	108,085,367 (GRCm39)	missense	probably damaging	0.97
fettfeld	UTSW	8	108,074,359 (GRCm39)	missense	probably damaging	1.00
Grunefeld	UTSW	8	108,082,140 (GRCm39)	splice site	probably null
Kleinfeld	UTSW	8	108,078,070 (GRCm39)	missense	probably damaging	1.00
Lisa	UTSW	8	108,074,321 (GRCm39)	missense	probably damaging	1.00
viola	UTSW	8	108,085,300 (GRCm39)	missense	probably damaging	1.00
H8562:Nfat5	UTSW	8	108,066,014 (GRCm39)	splice site	probably benign
R0003:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0117:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0118:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0119:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0135:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0138:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0141:Nfat5	UTSW	8	108,065,707 (GRCm39)	missense	probably damaging	1.00
R0302:Nfat5	UTSW	8	108,085,333 (GRCm39)	missense	probably damaging	1.00
R0420:Nfat5	UTSW	8	108,094,093 (GRCm39)	missense	probably damaging	1.00
R0613:Nfat5	UTSW	8	108,092,927 (GRCm39)	missense	possibly damaging	0.83
R0691:Nfat5	UTSW	8	108,082,237 (GRCm39)	missense	probably damaging	1.00
R0743:Nfat5	UTSW	8	108,094,698 (GRCm39)	missense	probably damaging	1.00
R1329:Nfat5	UTSW	8	108,095,659 (GRCm39)	missense	probably benign	0.42
R1550:Nfat5	UTSW	8	108,097,205 (GRCm39)	missense	probably damaging	0.99
R1590:Nfat5	UTSW	8	108,020,522 (GRCm39)	missense	probably damaging	1.00
R1827:Nfat5	UTSW	8	108,093,966 (GRCm39)	missense	probably benign	0.00
R1918:Nfat5	UTSW	8	108,092,868 (GRCm39)	missense	probably damaging	0.97
R2679:Nfat5	UTSW	8	108,071,546 (GRCm39)	missense	probably damaging	1.00
R2850:Nfat5	UTSW	8	108,020,492 (GRCm39)	missense	probably damaging	1.00
R3703:Nfat5	UTSW	8	108,078,053 (GRCm39)	splice site	probably benign
R3966:Nfat5	UTSW	8	108,093,921 (GRCm39)	missense	possibly damaging	0.47
R4301:Nfat5	UTSW	8	108,082,327 (GRCm39)	intron	probably benign
R4596:Nfat5	UTSW	8	108,078,132 (GRCm39)	missense	possibly damaging	0.93
R4602:Nfat5	UTSW	8	108,093,855 (GRCm39)	nonsense	probably null
R4627:Nfat5	UTSW	8	108,095,908 (GRCm39)	missense	probably damaging	1.00
R4917:Nfat5	UTSW	8	108,051,284 (GRCm39)	missense	probably damaging	1.00
R4918:Nfat5	UTSW	8	108,051,284 (GRCm39)	missense	probably damaging	1.00
R5089:Nfat5	UTSW	8	108,078,070 (GRCm39)	missense	probably damaging	1.00
R5495:Nfat5	UTSW	8	108,095,079 (GRCm39)	missense	probably benign	0.03
R5566:Nfat5	UTSW	8	108,095,767 (GRCm39)	missense	possibly damaging	0.47
R5851:Nfat5	UTSW	8	108,074,359 (GRCm39)	missense	probably damaging	1.00
R6012:Nfat5	UTSW	8	108,093,765 (GRCm39)	missense	probably benign	0.09
R6018:Nfat5	UTSW	8	108,082,283 (GRCm39)	critical splice donor site	probably null
R6364:Nfat5	UTSW	8	108,094,909 (GRCm39)	missense	probably benign	0.00
R6404:Nfat5	UTSW	8	108,097,220 (GRCm39)	missense	probably benign	0.01
R6466:Nfat5	UTSW	8	108,082,140 (GRCm39)	splice site	probably null
R7056:Nfat5	UTSW	8	108,094,738 (GRCm39)	missense	probably damaging	1.00
R7105:Nfat5	UTSW	8	108,095,823 (GRCm39)	missense	possibly damaging	0.88
R7128:Nfat5	UTSW	8	108,085,323 (GRCm39)	missense	probably benign	0.10
R7214:Nfat5	UTSW	8	108,020,515 (GRCm39)	missense	probably damaging	0.99
R7276:Nfat5	UTSW	8	108,093,731 (GRCm39)	missense	probably benign	0.25
R7560:Nfat5	UTSW	8	108,097,221 (GRCm39)	missense	probably benign	0.15
R7844:Nfat5	UTSW	8	108,085,300 (GRCm39)	missense	probably damaging	1.00
R7993:Nfat5	UTSW	8	108,082,134 (GRCm39)	splice site	probably null
R8407:Nfat5	UTSW	8	108,094,047 (GRCm39)	nonsense	probably null
R8428:Nfat5	UTSW	8	108,095,152 (GRCm39)	missense	probably damaging	0.96
R8798:Nfat5	UTSW	8	108,074,321 (GRCm39)	missense	probably damaging	1.00
R8919:Nfat5	UTSW	8	108,095,228 (GRCm39)	missense	probably damaging	0.99
R9067:Nfat5	UTSW	8	108,094,536 (GRCm39)	missense	probably benign	0.07
R9123:Nfat5	UTSW	8	108,078,141 (GRCm39)	missense	probably damaging	0.97
R9226:Nfat5	UTSW	8	108,095,401 (GRCm39)	missense	probably damaging	1.00
R9351:Nfat5	UTSW	8	108,065,910 (GRCm39)	missense	probably damaging	1.00
X0022:Nfat5	UTSW	8	108,074,388 (GRCm39)	nonsense	probably null
Z1177:Nfat5	UTSW	8	108,065,474 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTTCTAGTAAACAAGCCAATGACATGC -3'
(R):5'- GGGTTAAACTGAAGAAACCACACTCCTC -3'

Sequencing Primer
(F):5'- GCCAATGACATGCTAAGAAATTACTC -3'
(R):5'- catgatccccttgcctcc -3'

Posted On

2014-05-23