Mutagenetix > Incidental Mutation

Incidental Mutation 'R1738:Wdr91'

199860

Institutional Source

Beutler Lab

Gene Symbol

Wdr91

Ensembl Gene

ENSMUSG00000058486

Gene Name

WD repeat domain 91

Synonyms

9530020G05Rik

MMRRC Submission

039770-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.173) question?

Stock #

R1738 (G1)

Quality Score

199

Status

Not validated

Chromosome

Chromosomal Location

34857361-34887766 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 34861243 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 653 (P653L)

Ref Sequence

ENSEMBL: ENSMUSP00000079974 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081214] [ENSMUST00000146968]

AlphaFold

Q7TMQ7

Predicted Effect

probably damaging
Transcript: ENSMUST00000081214
AA Change: P653L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000079974
Gene: ENSMUSG00000058486
AA Change: P653L

Domain	Start	End	E-Value	Type
Blast:LisH	5	36	3e-6	BLAST
coiled coil region	179	228	N/A	INTRINSIC
low complexity region	263	279	N/A	INTRINSIC
WD40	393	437	6.34e-2	SMART
WD40	442	480	3.42e1	SMART
Blast:WD40	483	525	1e-19	BLAST
WD40	552	591	1.88e-4	SMART
WD40	594	633	3.96e-3	SMART
WD40	701	742	8.59e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000146968

SMART Domains

Protein: ENSMUSP00000138406
Gene: ENSMUSG00000058486

Domain	Start	End	E-Value	Type
Blast:LisH	5	36	2e-6	BLAST
coiled coil region	179	228	N/A	INTRINSIC
low complexity region	263	279	N/A	INTRINSIC
WD40	393	437	6.34e-2	SMART
WD40	442	480	3.42e1	SMART
Blast:WD40	483	525	1e-19	BLAST
WD40	552	591	1.48e-2	SMART

Coding Region Coverage

1x: 97.5%
3x: 96.8%
10x: 94.7%
20x: 89.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930562C15Rik	A	G	16: 4,682,475 (GRCm39)	Y202C	probably damaging	Het
Adamts16	G	A	13: 70,927,637 (GRCm39)		probably benign	Het
Adgrl3	T	G	5: 81,535,826 (GRCm39)	M155R	probably damaging	Het
Ak9	A	T	10: 41,211,917 (GRCm39)	Q218L	possibly damaging	Het
Akap13	G	A	7: 75,326,942 (GRCm39)	G647D	probably damaging	Het
Angptl3	A	G	4: 98,921,499 (GRCm39)	T206A	probably benign	Het
Arhgap19	A	G	19: 41,772,820 (GRCm39)	I291T	probably benign	Het
BC049715	C	T	6: 136,817,306 (GRCm39)	P182L	probably damaging	Het
Bsn	T	C	9: 107,984,133 (GRCm39)	D3307G	unknown	Het
Cby2	C	T	14: 75,830,497 (GRCm39)	M1I	probably null	Het
Ces2h	T	A	8: 105,745,697 (GRCm39)		probably null	Het
Ces4a	G	A	8: 105,864,729 (GRCm39)	G69S	probably damaging	Het
Col4a2	T	A	8: 11,496,238 (GRCm39)	F1620I	probably damaging	Het
Col6a3	T	C	1: 90,744,083 (GRCm39)	E88G	probably damaging	Het
Coro6	A	G	11: 77,360,251 (GRCm39)	D407G	probably benign	Het
Cpe	A	T	8: 65,064,475 (GRCm39)	F241L	probably damaging	Het
Csde1	A	G	3: 102,936,493 (GRCm39)		probably benign	Het
Cspg4b	T	G	13: 113,504,034 (GRCm39)	L179R	possibly damaging	Het
Dgki	A	G	6: 37,034,367 (GRCm39)	I361T	possibly damaging	Het
Dnah3	A	G	7: 119,634,582 (GRCm39)	V1298A	probably damaging	Het
Duox2	T	A	2: 122,123,895 (GRCm39)	I430F	probably damaging	Het
Gak	T	C	5: 108,764,842 (GRCm39)	Y153C	probably damaging	Het
Gal3st2	T	A	1: 93,802,318 (GRCm39)		probably null	Het
Gbp11	T	C	5: 105,474,510 (GRCm39)	N389D	probably benign	Het
Grm1	A	G	10: 10,812,163 (GRCm39)	V287A	probably damaging	Het
Hsp90ab1	T	C	17: 45,882,732 (GRCm39)	K36E	probably damaging	Het
Il24	T	A	1: 130,815,099 (GRCm39)		probably null	Het
Ino80d	T	C	1: 63,132,624 (GRCm39)	D13G	probably damaging	Het
Ipp	G	A	4: 116,387,618 (GRCm39)	V399I	probably benign	Het
Kank3	A	T	17: 34,036,168 (GRCm39)	D12V	probably damaging	Het
Lcn12	A	T	2: 25,383,263 (GRCm39)	S74R	probably damaging	Het
Mast3	T	C	8: 71,237,200 (GRCm39)	T637A	probably benign	Het
Nes	C	A	3: 87,883,728 (GRCm39)	Y662*	probably null	Het
Nudt13	A	T	14: 20,359,762 (GRCm39)	H163L	probably damaging	Het
Or1j17	T	C	2: 36,578,797 (GRCm39)	V261A	probably benign	Het
Or4a2	T	C	2: 89,248,362 (GRCm39)	T132A	probably benign	Het
Or4p7	G	A	2: 88,221,671 (GRCm39)	V27I	probably benign	Het
Or8k1	A	T	2: 86,048,060 (GRCm39)		probably null	Het
Pde6b	C	T	5: 108,578,425 (GRCm39)	R788*	probably null	Het
Pex13	A	G	11: 23,599,458 (GRCm39)	L351P	probably benign	Het
Phc1	A	G	6: 122,295,525 (GRCm39)	M942T	probably damaging	Het
Plch1	A	T	3: 63,626,659 (GRCm39)	D571E	probably benign	Het
Plec	C	T	15: 76,070,418 (GRCm39)	V931M	probably damaging	Het
Plekhh2	A	G	17: 84,874,125 (GRCm39)	N470S	possibly damaging	Het
Primpol	A	G	8: 47,060,873 (GRCm39)	S82P	probably damaging	Het
Prkg1	T	C	19: 30,764,322 (GRCm39)	D256G	possibly damaging	Het
Prune2	A	G	19: 17,102,374 (GRCm39)	E2511G	probably benign	Het
Rassf8	T	C	6: 145,761,034 (GRCm39)	I120T	probably benign	Het
Rfx4	G	A	10: 84,716,839 (GRCm39)		probably null	Het
Rtp2	A	T	16: 23,746,423 (GRCm39)	N69K	probably benign	Het
Sh3d19	A	T	3: 86,027,913 (GRCm39)	I597F	probably damaging	Het
Sorl1	T	A	9: 42,001,261 (GRCm39)	E246D	probably benign	Het
Tet2	T	A	3: 133,187,148 (GRCm39)	I1094L	probably benign	Het
Tlr4	A	T	4: 66,759,313 (GRCm39)	H702L	probably benign	Het
Tnfrsf14	T	C	4: 155,009,788 (GRCm39)	D47G	probably damaging	Het
Ttn	T	C	2: 76,777,157 (GRCm39)	Y1415C	probably damaging	Het
Vmn2r17	T	A	5: 109,576,377 (GRCm39)	F416Y	probably benign	Het
Vmn2r54	A	G	7: 12,369,815 (GRCm39)	S83P	probably benign	Het
Vtn	A	G	11: 78,390,422 (GRCm39)	D53G	possibly damaging	Het
Vwde	A	T	6: 13,190,723 (GRCm39)	V456D	probably damaging	Het
Ythdf1	G	A	2: 180,553,285 (GRCm39)	A283V	probably benign	Het
Zfp975	C	A	7: 42,312,373 (GRCm39)	W80L	probably benign	Het

Other mutations in Wdr91

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01336:Wdr91	APN	6	34,886,478 (GRCm39)	splice site	probably benign
IGL01340:Wdr91	APN	6	34,881,514 (GRCm39)	missense	probably benign	0.00
IGL01402:Wdr91	APN	6	34,865,998 (GRCm39)	missense	probably benign
IGL02632:Wdr91	APN	6	34,865,977 (GRCm39)	missense	probably damaging	1.00
IGL03104:Wdr91	APN	6	34,882,491 (GRCm39)	missense	probably benign	0.00
IGL03169:Wdr91	APN	6	34,882,426 (GRCm39)	missense	possibly damaging	0.93
R0080:Wdr91	UTSW	6	34,883,620 (GRCm39)	missense	possibly damaging	0.81
R0082:Wdr91	UTSW	6	34,883,620 (GRCm39)	missense	possibly damaging	0.81
R0422:Wdr91	UTSW	6	34,857,781 (GRCm39)	missense	probably damaging	1.00
R1993:Wdr91	UTSW	6	34,869,297 (GRCm39)	missense	probably damaging	1.00
R1994:Wdr91	UTSW	6	34,869,297 (GRCm39)	missense	probably damaging	1.00
R3115:Wdr91	UTSW	6	34,882,522 (GRCm39)	missense	probably damaging	0.98
R3116:Wdr91	UTSW	6	34,882,522 (GRCm39)	missense	probably damaging	0.98
R4261:Wdr91	UTSW	6	34,881,457 (GRCm39)	missense	possibly damaging	0.84
R4760:Wdr91	UTSW	6	34,885,234 (GRCm39)	missense	probably damaging	0.96
R4977:Wdr91	UTSW	6	34,887,726 (GRCm39)	missense	probably damaging	1.00
R5093:Wdr91	UTSW	6	34,869,288 (GRCm39)	missense	probably damaging	1.00
R5910:Wdr91	UTSW	6	34,868,422 (GRCm39)	missense	possibly damaging	0.93
R6788:Wdr91	UTSW	6	34,863,754 (GRCm39)	missense	probably damaging	0.99
R7139:Wdr91	UTSW	6	34,885,198 (GRCm39)	missense	possibly damaging	0.82
R7195:Wdr91	UTSW	6	34,866,209 (GRCm39)	missense	possibly damaging	0.70
R7268:Wdr91	UTSW	6	34,869,375 (GRCm39)	missense	probably benign
R7303:Wdr91	UTSW	6	34,861,258 (GRCm39)	missense	probably benign	0.01
R7326:Wdr91	UTSW	6	34,881,561 (GRCm39)	missense	probably damaging	0.99
R7341:Wdr91	UTSW	6	34,868,395 (GRCm39)	missense	possibly damaging	0.49
R7362:Wdr91	UTSW	6	34,866,050 (GRCm39)	missense	possibly damaging	0.50
R8098:Wdr91	UTSW	6	34,863,817 (GRCm39)	missense	possibly damaging	0.67
R9424:Wdr91	UTSW	6	34,861,302 (GRCm39)	missense	possibly damaging	0.64
R9664:Wdr91	UTSW	6	34,865,961 (GRCm39)	missense	probably benign	0.01
X0026:Wdr91	UTSW	6	34,886,307 (GRCm39)	missense	probably benign	0.03
Z1177:Wdr91	UTSW	6	34,868,435 (GRCm39)	missense	probably benign	0.30

Predicted Primers

PCR Primer
(F):5'- TCAACTCAGGTGAGCCAAGCTGAC -3'
(R):5'- AGGCTTTTCTGAAGGCTGTGGAAC -3'

Sequencing Primer
(F):5'- CAAGCTGACCTCCAGAGAGAG -3'
(R):5'- AGTCACTGCCCGAGGTTG -3'

Posted On

2014-05-23