Mutagenetix > Incidental Mutation

Incidental Mutation 'R1741:Gpr158'

200317

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

039773-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1741 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21372378-21835355 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 21832359 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 1153 (N1153S)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably benign
Transcript: ENSMUST00000055946
AA Change: N1153S

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: N1153S

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.5%
20x: 92.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034E13Rik	A	G	18: 52,793,578 (GRCm39)	N37S	probably damaging	Het
Acad10	T	C	5: 121,785,899 (GRCm39)	K230R	probably damaging	Het
Actl7a	A	G	4: 56,744,252 (GRCm39)	N260D	probably benign	Het
Adam24	T	C	8: 41,132,642 (GRCm39)	Y37H	probably benign	Het
Ahcy	G	A	2: 154,906,154 (GRCm39)	A229V	probably benign	Het
Ap3b2	A	G	7: 81,117,347 (GRCm39)	V563A	possibly damaging	Het
Bcl9l	T	A	9: 44,420,986 (GRCm39)	M1427K	probably damaging	Het
Btf3	C	T	13: 98,452,804 (GRCm39)	M1I	probably null	Het
Btg4	A	T	9: 51,027,910 (GRCm39)	I27L	probably benign	Het
Ccdc171	A	G	4: 83,539,076 (GRCm39)	Y366C	probably damaging	Het
Chd3	A	G	11: 69,246,480 (GRCm39)	Y1085H	probably damaging	Het
Cnot10	T	C	9: 114,426,892 (GRCm39)	D616G	possibly damaging	Het
Crlf1	C	A	8: 70,953,556 (GRCm39)	D243E	probably damaging	Het
Cyp2b23	A	G	7: 26,372,502 (GRCm39)	V371A	possibly damaging	Het
Dennd2a	A	G	6: 39,470,091 (GRCm39)	S534P	probably damaging	Het
Eln	A	G	5: 134,758,038 (GRCm39)	V185A	unknown	Het
Epor	C	T	9: 21,871,067 (GRCm39)	G301D	probably damaging	Het
Fam83f	T	G	15: 80,576,468 (GRCm39)	V373G	possibly damaging	Het
Fbxl21	A	T	13: 56,684,915 (GRCm39)	T340S	probably benign	Het
Fcgbpl1	G	T	7: 27,857,279 (GRCm39)	C2209F	probably damaging	Het
Fez1	T	A	9: 36,755,029 (GRCm39)	D9E	probably damaging	Het
Fsip2	T	C	2: 82,820,256 (GRCm39)	F5330L	probably benign	Het
Glis1	G	A	4: 107,425,544 (GRCm39)	R197Q	probably damaging	Het
Gm10277	TC	T	11: 77,676,828 (GRCm39)		probably null	Het
Gramd4	T	C	15: 85,975,730 (GRCm39)		probably null	Het
Hhatl	T	A	9: 121,618,125 (GRCm39)	Y210F	possibly damaging	Het
Hltf	T	C	3: 20,140,352 (GRCm39)	W422R	probably damaging	Het
Hspa5	T	C	2: 34,662,704 (GRCm39)	S87P	possibly damaging	Het
Il21	T	C	3: 37,281,811 (GRCm39)	H111R	probably benign	Het
Ip6k1	G	A	9: 107,918,183 (GRCm39)	G73S	probably benign	Het
Kdm5b	A	G	1: 134,545,755 (GRCm39)	D972G	possibly damaging	Het
Kif21b	C	T	1: 136,083,880 (GRCm39)	A709V	probably damaging	Het
Kmt2d	T	C	15: 98,743,115 (GRCm39)		probably benign	Het
Lrrc14b	A	G	13: 74,511,705 (GRCm39)	L125P	probably damaging	Het
Mapk8ip3	A	G	17: 25,118,828 (GRCm39)	S1169P	probably damaging	Het
Me3	A	T	7: 89,501,041 (GRCm39)	Y584F	probably damaging	Het
Mxra7	T	G	11: 116,707,070 (GRCm39)		probably null	Het
Nf1	T	C	11: 79,334,757 (GRCm39)	S870P	probably benign	Het
Npr2	G	A	4: 43,643,350 (GRCm39)	G525S	probably damaging	Het
Nyap1	A	T	5: 137,731,387 (GRCm39)	S726T	probably damaging	Het
Padi4	A	G	4: 140,473,481 (GRCm39)	V652A	probably damaging	Het
Pclo	A	G	5: 14,726,524 (GRCm39)		probably benign	Het
Pgm1	G	A	4: 99,822,062 (GRCm39)		probably null	Het
Piezo2	A	G	18: 63,154,244 (GRCm39)	S2512P	probably damaging	Het
Ptbp3	A	T	4: 59,482,624 (GRCm39)	D386E	probably damaging	Het
Ptk2	A	G	15: 73,114,255 (GRCm39)	V701A	possibly damaging	Het
Ptpn3	A	G	4: 57,254,922 (GRCm39)	V154A	probably damaging	Het
Rassf4	T	C	6: 116,616,450 (GRCm39)	E287G	probably damaging	Het
Rnh1	C	T	7: 140,743,936 (GRCm39)	R174H	probably benign	Het
Scn9a	T	A	2: 66,317,938 (GRCm39)	I1517F	probably damaging	Het
Sftpb	C	A	6: 72,282,797 (GRCm39)	A90E	probably benign	Het
Slc39a14	T	C	14: 70,556,193 (GRCm39)	K61R	probably damaging	Het
Tmem132d	A	C	5: 127,861,922 (GRCm39)	M733R	probably benign	Het
Tmem248	A	G	5: 130,265,664 (GRCm39)	I156V	probably benign	Het
Traf2	T	C	2: 25,414,495 (GRCm39)	D339G	probably damaging	Het
Trappc11	A	G	8: 47,982,362 (GRCm39)		probably null	Het
Tuba8	T	A	6: 121,199,727 (GRCm39)	I137N	possibly damaging	Het
Txlnb	A	G	10: 17,714,695 (GRCm39)	T376A	probably damaging	Het
Usp34	A	G	11: 23,314,103 (GRCm39)	T663A	probably benign	Het
Vmn2r26	T	A	6: 124,038,431 (GRCm39)	F669I	probably damaging	Het
Wdr95	G	A	5: 149,518,861 (GRCm39)		probably null	Het
Wfdc8	T	G	2: 164,450,789 (GRCm39)		probably benign	Het
Zfp64	A	C	2: 168,768,238 (GRCm39)	V458G	probably benign	Het
Zfp868	C	T	8: 70,064,519 (GRCm39)	G272D	probably damaging	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,373,494 (GRCm39)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,751,606 (GRCm39)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,751,584 (GRCm39)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,831,629 (GRCm39)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,653,832 (GRCm39)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,373,842 (GRCm39)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,831,909 (GRCm39)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,788,101 (GRCm39)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,373,511 (GRCm39)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,831,407 (GRCm39)	missense	probably benign
IGL02681:Gpr158	APN	2	21,820,441 (GRCm39)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,831,638 (GRCm39)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,831,890 (GRCm39)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,787,972 (GRCm39)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,831,057 (GRCm39)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,831,682 (GRCm39)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,815,479 (GRCm39)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,831,528 (GRCm39)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,830,019 (GRCm39)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,820,480 (GRCm39)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,831,125 (GRCm39)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,820,505 (GRCm39)	splice site	probably null
R1609:Gpr158	UTSW	2	21,788,104 (GRCm39)	missense	possibly damaging	0.61
R1827:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R1854:Gpr158	UTSW	2	21,373,935 (GRCm39)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,820,426 (GRCm39)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,832,325 (GRCm39)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R2275:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R3004:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,581,771 (GRCm39)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,373,370 (GRCm39)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,373,362 (GRCm39)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,830,025 (GRCm39)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,832,403 (GRCm39)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4506:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,373,811 (GRCm39)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,831,864 (GRCm39)	missense	probably benign
R4798:Gpr158	UTSW	2	21,787,993 (GRCm39)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,830,059 (GRCm39)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,831,968 (GRCm39)	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21,832,316 (GRCm39)	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21,831,101 (GRCm39)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,832,046 (GRCm39)	missense	probably benign
R5637:Gpr158	UTSW	2	21,788,083 (GRCm39)	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21,751,520 (GRCm39)	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21,373,331 (GRCm39)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,373,932 (GRCm39)	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21,373,319 (GRCm39)	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21,404,227 (GRCm39)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,820,422 (GRCm39)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,815,365 (GRCm39)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,831,099 (GRCm39)	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21,831,921 (GRCm39)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,653,802 (GRCm39)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,831,386 (GRCm39)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,373,113 (GRCm39)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,815,412 (GRCm39)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,581,750 (GRCm39)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R7515:Gpr158	UTSW	2	21,373,092 (GRCm39)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,831,158 (GRCm39)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R8311:Gpr158	UTSW	2	21,373,701 (GRCm39)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,581,693 (GRCm39)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,404,149 (GRCm39)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,558,137 (GRCm39)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,581,751 (GRCm39)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,581,760 (GRCm39)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,830,078 (GRCm39)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,831,251 (GRCm39)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,832,037 (GRCm39)	missense	probably benign
R9379:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,787,972 (GRCm39)	missense	probably benign
R9497:Gpr158	UTSW	2	21,831,825 (GRCm39)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,830,054 (GRCm39)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,831,315 (GRCm39)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,831,180 (GRCm39)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,815,501 (GRCm39)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,832,083 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GGAGAATGGAAGCCAGCTCTACAC -3'
(R):5'- GGCAGAGTCAGCCTTCCTTCTTATG -3'

Sequencing Primer
(F):5'- CAACCAATATGTGTGCTGGGC -3'
(R):5'- CTAGGCTAACAAAGATGCCTTG -3'

Posted On

2014-05-23