Incidental Mutation 'R1742:Fcrl2'
ID 200405
Institutional Source Beutler Lab
Gene Symbol Fcrl2
Ensembl Gene ENSMUSG00000015852
Gene Name Fc receptor like 2
Synonyms Fcrls, IFGP2, 2810439C17Rik, Msr2, Fcrh2, moFcRH2sc
MMRRC Submission 039774-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.053) question?
Stock # R1742 (G1)
Quality Score 225
Status Not validated
Chromosome 3
Chromosomal Location 87158318-87171046 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 87166350 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 142 (T142A)
Ref Sequence ENSEMBL: ENSMUSP00000088508 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000090986] [ENSMUST00000146512]
AlphaFold Q9EQY5
Predicted Effect possibly damaging
Transcript: ENSMUST00000090986
AA Change: T142A

PolyPhen 2 Score 0.760 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000088508
Gene: ENSMUSG00000015852
AA Change: T142A

DomainStartEndE-ValueType
IG 23 106 1.32e-3 SMART
IGc2 122 186 2.77e-6 SMART
IGc2 226 291 1.09e-4 SMART
IG 315 396 1e-3 SMART
SR 402 503 7.29e-36 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000146512
AA Change: T142A

PolyPhen 2 Score 0.708 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000115780
Gene: ENSMUSG00000015852
AA Change: T142A

DomainStartEndE-ValueType
IG 23 106 1.32e-3 SMART
Pfam:Ig_2 111 176 6.1e-6 PFAM
Pfam:Ig_3 111 176 1.4e-4 PFAM
Coding Region Coverage
  • 1x: 97.5%
  • 3x: 96.9%
  • 10x: 95.4%
  • 20x: 92.7%
Validation Efficiency
MGI Phenotype PHENOTYPE: Female homozygous mutant mice are larger than controls and show increased mean body weight, total tissue mass, lean body mass and total body fat. Homozygous mutant mice eshow a decreased mean percentage of CD8 cells in the peripheral blood. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ankhd1 C A 18: 36,758,318 (GRCm39) A1004E probably damaging Het
Arfgef2 A G 2: 166,708,900 (GRCm39) S1071G probably damaging Het
Arhgef5 T A 6: 43,257,133 (GRCm39) I1228N probably damaging Het
Auh G A 13: 52,989,532 (GRCm39) P308L probably benign Het
Bptf A G 11: 107,001,777 (GRCm39) V445A probably damaging Het
Btf3 C T 13: 98,452,804 (GRCm39) M1I probably null Het
Bves C T 10: 45,223,961 (GRCm39) T207M probably damaging Het
Ccdc171 T A 4: 83,599,521 (GRCm39) S779T probably damaging Het
Ccdc54 T C 16: 50,410,601 (GRCm39) K222E possibly damaging Het
Cebpe A G 14: 54,949,057 (GRCm39) V120A probably benign Het
Clhc1 T A 11: 29,507,647 (GRCm39) probably null Het
Col22a1 C T 15: 71,673,762 (GRCm39) G985S unknown Het
Col6a3 T A 1: 90,741,516 (GRCm39) I639F probably damaging Het
Cryga C A 1: 65,142,280 (GRCm39) V38L probably benign Het
Dll3 T C 7: 27,993,848 (GRCm39) T530A probably benign Het
Dnaaf9 A T 2: 130,582,315 (GRCm39) probably null Het
Dnah7a G A 1: 53,495,843 (GRCm39) P3205S probably benign Het
Dpp10 T A 1: 123,372,935 (GRCm39) Y224F probably damaging Het
Eif1ad10 C T 12: 88,216,453 (GRCm39) D140N unknown Het
Fyttd1 C T 16: 32,725,923 (GRCm39) R175* probably null Het
Gm10277 TC T 11: 77,676,828 (GRCm39) probably null Het
Gpr33 T C 12: 52,071,045 (GRCm39) probably null Het
Gse1 T A 8: 121,293,689 (GRCm39) V205E probably damaging Het
Herc4 C A 10: 63,123,728 (GRCm39) N461K probably benign Het
Ifi206 G A 1: 173,309,537 (GRCm39) T153I probably benign Het
Iqca1 T C 1: 90,025,773 (GRCm39) I341V probably benign Het
Itsn1 T G 16: 91,613,847 (GRCm39) probably null Het
Kcnk5 T A 14: 20,191,925 (GRCm39) Y412F probably benign Het
Lemd1 T A 1: 132,156,036 (GRCm39) I26K probably damaging Het
Lipc A T 9: 70,727,811 (GRCm39) L12Q probably damaging Het
Lrrtm1 C A 6: 77,221,074 (GRCm39) P177Q probably damaging Het
Mcph1 G A 8: 18,657,379 (GRCm39) G73R probably benign Het
Msantd5f6 T C 4: 73,319,447 (GRCm39) D99G probably damaging Het
Myh11 A T 16: 14,037,908 (GRCm39) L899Q probably damaging Het
Myo18a G T 11: 77,732,293 (GRCm39) R822L probably damaging Het
Nav3 T C 10: 109,605,074 (GRCm39) T1000A probably benign Het
Nox4 T C 7: 86,945,026 (GRCm39) V94A possibly damaging Het
Or10ag53 T A 2: 87,083,122 (GRCm39) N280K probably benign Het
Or4c123 G T 2: 89,126,768 (GRCm39) P282H probably damaging Het
Or7g32 A G 9: 19,389,337 (GRCm39) S67P probably damaging Het
Oxr1 T C 15: 41,713,955 (GRCm39) L679P probably damaging Het
Pcdhb17 T C 18: 37,619,629 (GRCm39) I473T probably damaging Het
Pgbd5 T A 8: 125,107,046 (GRCm39) E165D probably damaging Het
Pgpep1l A T 7: 67,886,802 (GRCm39) V169D probably damaging Het
Phf12 C T 11: 77,900,312 (GRCm39) T136I probably benign Het
Pif1 T A 9: 65,495,132 (GRCm39) M14K probably benign Het
Pigr A G 1: 130,772,823 (GRCm39) E347G probably damaging Het
Plekha3 G A 2: 76,513,223 (GRCm39) E103K possibly damaging Het
Ptgs2 A G 1: 149,980,150 (GRCm39) I363V probably damaging Het
Rasl11a T A 5: 146,783,805 (GRCm39) probably null Het
Recql T C 6: 142,310,298 (GRCm39) T511A probably damaging Het
Rgl2 T A 17: 34,156,197 (GRCm39) probably null Het
Rpp25l T C 4: 41,712,763 (GRCm39) Y4C probably damaging Het
Sass6 T G 3: 116,401,126 (GRCm39) C156G probably damaging Het
Sgta T G 10: 80,882,111 (GRCm39) N288T probably damaging Het
Slco1a4 T A 6: 141,770,771 (GRCm39) T282S probably benign Het
Smad4 T C 18: 73,808,968 (GRCm39) R100G probably damaging Het
Sox8 C T 17: 25,786,915 (GRCm39) V263M probably damaging Het
Sp8 A G 12: 118,813,552 (GRCm39) H469R probably benign Het
Spata1 A T 3: 146,175,378 (GRCm39) probably null Het
Taar7a G A 10: 23,869,117 (GRCm39) S88F probably damaging Het
Tnks2 T C 19: 36,853,661 (GRCm39) L749S probably damaging Het
Tollip C A 7: 141,446,592 (GRCm39) R19L probably damaging Het
Tox2 G A 2: 163,067,446 (GRCm39) R55H probably benign Het
Vmn2r27 T C 6: 124,177,636 (GRCm39) E456G possibly damaging Het
Vmn2r77 T A 7: 86,444,543 (GRCm39) N65K probably benign Het
Vwf T C 6: 125,644,513 (GRCm39) M2456T probably benign Het
Zfp526 A G 7: 24,923,939 (GRCm39) N66S possibly damaging Het
Zic1 T C 9: 91,243,629 (GRCm39) Y446C probably damaging Het
Other mutations in Fcrl2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01568:Fcrl2 APN 3 87,163,986 (GRCm39) missense probably damaging 0.99
IGL01959:Fcrl2 APN 3 87,166,939 (GRCm39) missense probably damaging 0.97
IGL02409:Fcrl2 APN 3 87,160,030 (GRCm39) missense probably benign 0.00
IGL02677:Fcrl2 APN 3 87,166,694 (GRCm39) missense probably benign 0.01
IGL02957:Fcrl2 APN 3 87,169,501 (GRCm39) missense possibly damaging 0.59
IGL02974:Fcrl2 APN 3 87,164,704 (GRCm39) missense possibly damaging 0.89
IGL02992:Fcrl2 APN 3 87,166,773 (GRCm39) missense probably damaging 0.99
BB001:Fcrl2 UTSW 3 87,166,840 (GRCm39) missense probably damaging 0.99
BB011:Fcrl2 UTSW 3 87,166,840 (GRCm39) missense probably damaging 0.99
R0052:Fcrl2 UTSW 3 87,164,085 (GRCm39) missense possibly damaging 0.94
R0052:Fcrl2 UTSW 3 87,164,085 (GRCm39) missense possibly damaging 0.94
R0131:Fcrl2 UTSW 3 87,166,266 (GRCm39) missense possibly damaging 0.90
R1171:Fcrl2 UTSW 3 87,164,167 (GRCm39) missense probably benign 0.24
R1319:Fcrl2 UTSW 3 87,169,484 (GRCm39) critical splice donor site probably null
R1522:Fcrl2 UTSW 3 87,164,014 (GRCm39) missense possibly damaging 0.64
R1696:Fcrl2 UTSW 3 87,166,825 (GRCm39) missense possibly damaging 0.95
R2156:Fcrl2 UTSW 3 87,164,648 (GRCm39) missense probably benign 0.43
R2255:Fcrl2 UTSW 3 87,164,655 (GRCm39) nonsense probably null
R2257:Fcrl2 UTSW 3 87,166,928 (GRCm39) missense probably damaging 0.99
R2434:Fcrl2 UTSW 3 87,164,005 (GRCm39) missense probably damaging 1.00
R2680:Fcrl2 UTSW 3 87,164,656 (GRCm39) missense probably damaging 0.99
R3552:Fcrl2 UTSW 3 87,166,717 (GRCm39) missense possibly damaging 0.73
R4866:Fcrl2 UTSW 3 87,170,773 (GRCm39) missense possibly damaging 0.65
R4883:Fcrl2 UTSW 3 87,166,922 (GRCm39) missense possibly damaging 0.48
R5654:Fcrl2 UTSW 3 87,164,851 (GRCm39) missense probably benign
R5771:Fcrl2 UTSW 3 87,170,775 (GRCm39) missense probably damaging 0.98
R5917:Fcrl2 UTSW 3 87,164,094 (GRCm39) missense probably damaging 0.99
R6349:Fcrl2 UTSW 3 87,159,803 (GRCm39) missense probably damaging 0.99
R6562:Fcrl2 UTSW 3 87,164,635 (GRCm39) missense probably benign
R6954:Fcrl2 UTSW 3 87,170,983 (GRCm39) critical splice donor site probably benign
R7059:Fcrl2 UTSW 3 87,164,647 (GRCm39) missense possibly damaging 0.82
R7188:Fcrl2 UTSW 3 87,166,830 (GRCm39) missense probably benign 0.13
R7201:Fcrl2 UTSW 3 87,159,934 (GRCm39) missense probably damaging 0.99
R7369:Fcrl2 UTSW 3 87,164,008 (GRCm39) missense possibly damaging 0.59
R7431:Fcrl2 UTSW 3 87,166,233 (GRCm39) missense probably damaging 0.99
R7610:Fcrl2 UTSW 3 87,160,004 (GRCm39) missense probably damaging 1.00
R7924:Fcrl2 UTSW 3 87,166,840 (GRCm39) missense probably damaging 0.99
R8018:Fcrl2 UTSW 3 87,166,933 (GRCm39) nonsense probably null
R8280:Fcrl2 UTSW 3 87,166,364 (GRCm39) nonsense probably null
R8981:Fcrl2 UTSW 3 87,164,677 (GRCm39) missense probably damaging 1.00
R9368:Fcrl2 UTSW 3 87,164,906 (GRCm39) missense possibly damaging 0.59
R9477:Fcrl2 UTSW 3 87,159,803 (GRCm39) missense probably damaging 0.98
R9522:Fcrl2 UTSW 3 87,164,101 (GRCm39) missense possibly damaging 0.95
Predicted Primers PCR Primer
(F):5'- AGCATTCAAGAGTCCCGATGAGCC -3'
(R):5'- GCTGTGTCTAGGAAAGAGAGTGCC -3'

Sequencing Primer
(F):5'- GATGAGCCATGATGTCTCCCAC -3'
(R):5'- ATAAATGGTAGTAGGCTGTCTGTCC -3'
Posted On 2014-05-23