Mutagenetix > Incidental Mutation

Incidental Mutation 'Y4340:Zfp28'

200615

Institutional Source

Beutler Lab

Gene Symbol

Zfp28

Ensembl Gene

ENSMUSG00000062861

Gene Name

zinc finger protein 28

Synonyms

2810438M17Rik, mkr-5, Zfp-28

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

Y4340 ()

Quality Score

182

Status

Not validated

Chromosome

Chromosomal Location

6386317-6399636 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 6397656 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Asparagine at position 697 (T697N)

Ref Sequence

ENSEMBL: ENSMUSP00000079812 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081022] [ENSMUST00000161855]

AlphaFold

P10078

Predicted Effect

probably damaging
Transcript: ENSMUST00000081022
AA Change: T697N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000079812
Gene: ENSMUSG00000062861
AA Change: T697N

Domain	Start	End	E-Value	Type
low complexity region	27	50	N/A	INTRINSIC
KRAB	103	163	3.53e-33	SMART
ZnF_C2H2	377	399	3.95e-4	SMART
ZnF_C2H2	405	427	6.88e-4	SMART
ZnF_C2H2	433	456	1.2e-3	SMART
ZnF_C2H2	462	484	9.58e-3	SMART
ZnF_C2H2	490	512	2.57e-3	SMART
ZnF_C2H2	518	540	1.82e-3	SMART
ZnF_C2H2	546	568	2.4e-3	SMART
ZnF_C2H2	574	596	6.32e-3	SMART
ZnF_C2H2	602	624	1.38e-3	SMART
ZnF_C2H2	630	652	4.87e-4	SMART
ZnF_C2H2	658	680	2.91e-2	SMART
ZnF_C2H2	686	708	2.36e-2	SMART
ZnF_C2H2	714	736	6.42e-4	SMART
ZnF_C2H2	742	764	2.4e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159318

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160568

Predicted Effect

probably benign
Transcript: ENSMUST00000161855

SMART Domains

Protein: ENSMUSP00000130202
Gene: ENSMUSG00000093536

Domain	Start	End	E-Value	Type
low complexity region	57	72	N/A	INTRINSIC
transmembrane domain	85	107	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000207086

Predicted Effect

probably benign
Transcript: ENSMUST00000207465

Predicted Effect

probably benign
Transcript: ENSMUST00000207809

Predicted Effect

probably benign
Transcript: ENSMUST00000208949

Predicted Effect

probably benign
Transcript: ENSMUST00000208338

Coding Region Coverage

1x: 97.4%
3x: 96.9%
10x: 95.5%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 2 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Dennd11	T	A	6: 40,412,992 (GRCm39)	Q23L	probably benign	Het
Ush2a	C	T	1: 188,475,826 (GRCm39)	T2921M	possibly damaging	Het

Other mutations in Zfp28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00737:Zfp28	APN	7	6,396,429 (GRCm39)	makesense	probably null
IGL02300:Zfp28	APN	7	6,392,495 (GRCm39)	missense	probably benign	0.00
IGL02541:Zfp28	APN	7	6,396,479 (GRCm39)	nonsense	probably null
FR4340:Zfp28	UTSW	7	6,397,862 (GRCm39)	missense	probably damaging	1.00
FR4342:Zfp28	UTSW	7	6,397,862 (GRCm39)	missense	probably damaging	1.00
R0442:Zfp28	UTSW	7	6,397,998 (GRCm39)	missense	probably damaging	1.00
R0462:Zfp28	UTSW	7	6,395,239 (GRCm39)	missense	possibly damaging	0.71
R0799:Zfp28	UTSW	7	6,387,182 (GRCm39)	missense	possibly damaging	0.49
R1081:Zfp28	UTSW	7	6,392,779 (GRCm39)	missense	possibly damaging	0.93
R1674:Zfp28	UTSW	7	6,397,942 (GRCm39)	missense	possibly damaging	0.90
R1783:Zfp28	UTSW	7	6,397,791 (GRCm39)	missense	probably damaging	1.00
R2119:Zfp28	UTSW	7	6,397,875 (GRCm39)	missense	probably benign	0.00
R2186:Zfp28	UTSW	7	6,397,497 (GRCm39)	missense	probably damaging	1.00
R4280:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4281:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4283:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4331:Zfp28	UTSW	7	6,396,700 (GRCm39)	missense	probably benign	0.07
R4379:Zfp28	UTSW	7	6,396,441 (GRCm39)	missense	probably benign	0.11
R4380:Zfp28	UTSW	7	6,396,441 (GRCm39)	missense	probably benign	0.11
R4505:Zfp28	UTSW	7	6,397,160 (GRCm39)	missense	probably damaging	1.00
R4659:Zfp28	UTSW	7	6,396,506 (GRCm39)	missense	probably benign	0.05
R4706:Zfp28	UTSW	7	6,392,793 (GRCm39)	missense	probably damaging	0.99
R5524:Zfp28	UTSW	7	6,397,850 (GRCm39)	splice site	probably null
R6269:Zfp28	UTSW	7	6,396,612 (GRCm39)	missense	probably benign	0.00
R6981:Zfp28	UTSW	7	6,397,692 (GRCm39)	missense	probably damaging	1.00
R7117:Zfp28	UTSW	7	6,397,461 (GRCm39)	missense	probably damaging	1.00
R7176:Zfp28	UTSW	7	6,386,456 (GRCm39)	missense	possibly damaging	0.49
R7312:Zfp28	UTSW	7	6,386,593 (GRCm39)	unclassified	probably benign
R7422:Zfp28	UTSW	7	6,397,748 (GRCm39)	missense	probably damaging	1.00
R7423:Zfp28	UTSW	7	6,396,956 (GRCm39)	missense	probably damaging	1.00
R7937:Zfp28	UTSW	7	6,396,785 (GRCm39)	missense	probably damaging	1.00
R8110:Zfp28	UTSW	7	6,392,828 (GRCm39)	missense	probably benign	0.02
R8704:Zfp28	UTSW	7	6,397,637 (GRCm39)	missense	probably damaging	1.00
R8804:Zfp28	UTSW	7	6,393,399 (GRCm39)	missense	probably damaging	0.98
R8854:Zfp28	UTSW	7	6,397,938 (GRCm39)	missense	probably benign	0.01
R9071:Zfp28	UTSW	7	6,397,544 (GRCm39)	missense	probably damaging	1.00
R9235:Zfp28	UTSW	7	6,397,605 (GRCm39)	missense	probably damaging	1.00
R9276:Zfp28	UTSW	7	6,397,440 (GRCm39)	missense	probably damaging	1.00
R9589:Zfp28	UTSW	7	6,392,816 (GRCm39)	missense	probably benign	0.09
R9600:Zfp28	UTSW	7	6,397,917 (GRCm39)	missense	probably benign	0.00
R9651:Zfp28	UTSW	7	6,395,623 (GRCm39)	missense
R9653:Zfp28	UTSW	7	6,395,623 (GRCm39)	missense
R9712:Zfp28	UTSW	7	6,396,878 (GRCm39)	missense	probably damaging	1.00
Z1177:Zfp28	UTSW	7	6,387,186 (GRCm39)	missense	probably benign	0.28

Predicted Primers

Posted On

2014-05-23