Mutagenetix > Incidental Mutation

Incidental Mutation 'R1574:Vmn1r42'

202717

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r42

Ensembl Gene

ENSMUSG00000068232

Gene Name

vomeronasal 1 receptor 42

Synonyms

V1ra6

MMRRC Submission

045014-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R1574 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89821500-89822597 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 89822059 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 170 (I170T)

Ref Sequence

ENSEMBL: ENSMUSP00000154442 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089419] [ENSMUST00000226436] [ENSMUST00000227279]

AlphaFold

Q8VBS7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000089419
AA Change: I170T

PolyPhen 2 Score 0.622 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000086840
Gene: ENSMUSG00000068232
AA Change: I170T

Domain	Start	End	E-Value	Type
Pfam:V1R	54	318	3.6e-132	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226436
AA Change: I170T

PolyPhen 2 Score 0.622 (Sensitivity: 0.87; Specificity: 0.91)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000227279
AA Change: I170T

PolyPhen 2 Score 0.622 (Sensitivity: 0.87; Specificity: 0.91)

Coding Region Coverage

1x: 97.5%
3x: 97.0%
10x: 95.5%
20x: 93.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl3	T	A	5: 81,935,296 (GRCm39)	N1276K	probably damaging	Het
Als2cl	A	G	9: 110,713,128 (GRCm39)	E6G	probably damaging	Het
Ankrd12	A	T	17: 66,293,269 (GRCm39)	D721E	probably benign	Het
Anpep	A	G	7: 79,488,155 (GRCm39)		probably null	Het
Apeh	A	T	9: 107,969,925 (GRCm39)		probably null	Het
Apob	A	T	12: 8,040,839 (GRCm39)	I655L	possibly damaging	Het
Atp2b1	T	A	10: 98,832,810 (GRCm39)	L437Q	probably damaging	Het
Cacna2d3	T	A	14: 29,073,779 (GRCm39)	R222S	probably damaging	Het
Cdc25b	T	A	2: 131,033,057 (GRCm39)		probably benign	Het
Cdon	A	G	9: 35,364,233 (GRCm39)		probably benign	Het
Cenpf	C	T	1: 189,384,910 (GRCm39)	D2457N	probably damaging	Het
Cenpo	A	T	12: 4,265,433 (GRCm39)		probably null	Het
Ces2b	G	T	8: 105,562,521 (GRCm39)	A284S	probably benign	Het
Clock	T	C	5: 76,390,679 (GRCm39)	D311G	probably damaging	Het
Csmd3	T	C	15: 47,559,257 (GRCm39)		probably null	Het
Dbil5	A	G	11: 76,109,308 (GRCm39)	M71V	probably benign	Het
Ddhd1	A	C	14: 45,833,004 (GRCm39)	L864R	probably damaging	Het
Ddx19a	T	C	8: 111,719,743 (GRCm39)		probably benign	Het
Dnah11	A	G	12: 118,024,052 (GRCm39)	C1900R	probably damaging	Het
Dnah2	A	G	11: 69,405,514 (GRCm39)	V666A	probably benign	Het
Dnah5	T	A	15: 28,252,569 (GRCm39)	M754K	probably benign	Het
Dnajc15	A	T	14: 78,063,854 (GRCm39)	S145T	probably benign	Het
Drap1	A	G	19: 5,474,285 (GRCm39)	F25S	probably damaging	Het
Drgx	A	G	14: 32,327,281 (GRCm39)		probably benign	Het
Fam83e	G	A	7: 45,376,135 (GRCm39)	E283K	probably damaging	Het
Fbxo48	G	T	11: 16,903,368 (GRCm39)		probably benign	Het
Fndc3a	A	T	14: 72,793,997 (GRCm39)	I892N	probably damaging	Het
Gcn1	A	G	5: 115,753,611 (GRCm39)	T2321A	probably benign	Het
Gm1110	G	T	9: 26,792,422 (GRCm39)		probably benign	Het
Gm5499	T	C	17: 87,386,423 (GRCm39)		noncoding transcript	Het
Gmnc	G	T	16: 26,782,729 (GRCm39)		probably benign	Het
Greb1l	A	G	18: 10,554,997 (GRCm39)	D1681G	possibly damaging	Het
Hc	G	A	2: 34,890,777 (GRCm39)		probably benign	Het
Hmcn2	A	C	2: 31,294,899 (GRCm39)	T2563P	probably damaging	Het
Iqcd	A	T	5: 120,738,300 (GRCm39)	K39N	probably damaging	Het
Irag2	T	G	6: 145,104,356 (GRCm39)		probably benign	Het
Kank2	A	G	9: 21,685,871 (GRCm39)	S668P	probably damaging	Het
Kcng1	T	A	2: 168,110,961 (GRCm39)	N68Y	probably damaging	Het
Kmt5b	T	A	19: 3,836,633 (GRCm39)		probably null	Het
Lama2	T	A	10: 27,200,750 (GRCm39)	I533F	possibly damaging	Het
Lcmt1	T	A	7: 123,002,131 (GRCm39)	I132N	probably damaging	Het
Mad2l2	G	T	4: 148,227,429 (GRCm39)		probably benign	Het
Mcph1	T	C	8: 18,851,428 (GRCm39)	I807T	probably damaging	Het
Mdn1	A	G	4: 32,722,315 (GRCm39)	I2366V	probably benign	Het
Moxd1	T	C	10: 24,176,217 (GRCm39)	W558R	probably damaging	Het
Myh13	T	C	11: 67,253,407 (GRCm39)		probably benign	Het
Myrf	T	C	19: 10,202,851 (GRCm39)	D141G	probably damaging	Het
Ncoa7	T	C	10: 30,570,097 (GRCm39)	I249M	probably damaging	Het
Obox5	T	C	7: 15,492,558 (GRCm39)	V171A	probably damaging	Het
Or13e8	A	T	4: 43,697,134 (GRCm39)	V13D	possibly damaging	Het
Or2c1	T	C	16: 3,657,521 (GRCm39)	I228T	probably damaging	Het
Or5ak20	A	T	2: 85,184,243 (GRCm39)	V9E	probably damaging	Het
Or6c219	A	G	10: 129,781,379 (GRCm39)	L69P	probably damaging	Het
Or7a36	C	A	10: 78,819,820 (GRCm39)	N32K	probably damaging	Het
Parp4	A	G	14: 56,839,752 (GRCm39)	T487A	probably damaging	Het
Pclo	A	G	5: 14,729,845 (GRCm39)		probably benign	Het
Pcnx2	G	A	8: 126,500,669 (GRCm39)	R1474C	probably damaging	Het
Qtrt2	A	G	16: 43,692,195 (GRCm39)		probably benign	Het
Sart1	G	A	19: 5,430,287 (GRCm39)	P788L	probably damaging	Het
Sdk1	A	G	5: 141,984,634 (GRCm39)	T740A	probably benign	Het
Serpinb1c	T	C	13: 33,072,979 (GRCm39)	D61G	possibly damaging	Het
Slc24a5	G	A	2: 124,922,782 (GRCm39)	G152S	probably damaging	Het
Slc6a4	A	T	11: 76,910,022 (GRCm39)	I426F	possibly damaging	Het
Stk33	C	T	7: 108,879,027 (GRCm39)	V441I	probably benign	Het
Sult1c2	A	G	17: 54,143,927 (GRCm39)		probably null	Het
Tdpoz4	T	A	3: 93,703,835 (GRCm39)	V44E	probably benign	Het
Tmprss13	C	A	9: 45,254,529 (GRCm39)	T432K	probably damaging	Het
Traf7	A	G	17: 24,729,527 (GRCm39)	L428P	probably damaging	Het
Tubb1	T	C	2: 174,299,215 (GRCm39)	I299T	probably benign	Het
Vmn1r158	A	T	7: 22,489,772 (GRCm39)	W146R	probably damaging	Het
Vmn2r116	A	T	17: 23,606,063 (GRCm39)	H325L	probably damaging	Het
Zfp516	T	A	18: 83,011,300 (GRCm39)	L1111H	possibly damaging	Het
Zfp61	C	G	7: 23,990,635 (GRCm39)	K505N	probably damaging	Het
Zfp653	C	A	9: 21,969,274 (GRCm39)	E331*	probably null	Het
Zfp949	A	T	9: 88,451,830 (GRCm39)	K467*	probably null	Het

Other mutations in Vmn1r42

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02055:Vmn1r42	APN	6	89,822,571 (GRCm39)	utr 5 prime	probably benign
IGL02151:Vmn1r42	APN	6	89,822,023 (GRCm39)	missense	possibly damaging	0.90
IGL02158:Vmn1r42	APN	6	89,822,296 (GRCm39)	missense	probably damaging	0.96
IGL02731:Vmn1r42	APN	6	89,822,407 (GRCm39)	missense	probably benign	0.41
IGL02738:Vmn1r42	APN	6	89,821,630 (GRCm39)	missense	possibly damaging	0.69
IGL02817:Vmn1r42	APN	6	89,822,518 (GRCm39)	missense	probably damaging	1.00
volkan	UTSW	6	89,821,949 (GRCm39)	missense	probably benign	0.00
R1131:Vmn1r42	UTSW	6	89,822,551 (GRCm39)	missense	possibly damaging	0.88
R1500:Vmn1r42	UTSW	6	89,822,483 (GRCm39)	missense	probably benign	0.01
R1557:Vmn1r42	UTSW	6	89,821,733 (GRCm39)	missense	possibly damaging	0.66
R1561:Vmn1r42	UTSW	6	89,822,363 (GRCm39)	missense	probably damaging	0.99
R1574:Vmn1r42	UTSW	6	89,822,363 (GRCm39)	missense	probably damaging	0.99
R1574:Vmn1r42	UTSW	6	89,822,059 (GRCm39)	missense	possibly damaging	0.62
R1857:Vmn1r42	UTSW	6	89,821,597 (GRCm39)	missense	probably benign	0.28
R1858:Vmn1r42	UTSW	6	89,821,597 (GRCm39)	missense	probably benign	0.28
R1916:Vmn1r42	UTSW	6	89,821,949 (GRCm39)	missense	probably benign	0.00
R2284:Vmn1r42	UTSW	6	89,821,681 (GRCm39)	missense	probably benign	0.26
R2912:Vmn1r42	UTSW	6	89,821,688 (GRCm39)	missense	probably benign
R4541:Vmn1r42	UTSW	6	89,822,533 (GRCm39)	missense	probably benign
R5085:Vmn1r42	UTSW	6	89,821,598 (GRCm39)	missense	probably benign	0.00
R5384:Vmn1r42	UTSW	6	89,822,366 (GRCm39)	missense	probably damaging	1.00
R5616:Vmn1r42	UTSW	6	89,822,084 (GRCm39)	missense	possibly damaging	0.66
R5647:Vmn1r42	UTSW	6	89,822,314 (GRCm39)	missense	probably benign	0.43
R5867:Vmn1r42	UTSW	6	89,821,761 (GRCm39)	nonsense	probably null
R6569:Vmn1r42	UTSW	6	89,822,425 (GRCm39)	missense	probably damaging	1.00
R6852:Vmn1r42	UTSW	6	89,821,769 (GRCm39)	missense	possibly damaging	0.71
R7054:Vmn1r42	UTSW	6	89,822,051 (GRCm39)	missense	possibly damaging	0.90
R7399:Vmn1r42	UTSW	6	89,822,495 (GRCm39)	missense	probably benign	0.27
R7958:Vmn1r42	UTSW	6	89,822,059 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTTCCATGAAGATGCTGTGTC -3'
(R):5'- ACCTGTACAGAGTTTTGAGGGG -3'

Sequencing Primer
(F):5'- GTGCATGCTTAAGAGGGCTACC -3'
(R):5'- ACAGAGTTTTGAGGGGTTTCTCC -3'

Posted On

2014-06-23