Incidental Mutation 'R1817:Mical3'
ID 204490
Institutional Source Beutler Lab
Gene Symbol Mical3
Ensembl Gene ENSMUSG00000051586
Gene Name microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms C130040D16Rik, MICAL-3
MMRRC Submission 039845-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.154) question?
Stock # R1817 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 120908668-121107959 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to G at 121019196 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Proline at position 9 (T9P)
Ref Sequence ENSEMBL: ENSMUSP00000146780 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000077159] [ENSMUST00000203254] [ENSMUST00000204248] [ENSMUST00000207889] [ENSMUST00000207968] [ENSMUST00000207993]
AlphaFold Q8CJ19
Predicted Effect probably benign
Transcript: ENSMUST00000077159
AA Change: T9P

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000076402
Gene: ENSMUSG00000051586
AA Change: T9P

DomainStartEndE-ValueType
low complexity region 60 69 N/A INTRINSIC
Pfam:FAD_binding_3 86 142 1.4e-7 PFAM
low complexity region 162 175 N/A INTRINSIC
CH 520 619 4.44e-17 SMART
low complexity region 625 635 N/A INTRINSIC
LIM 763 815 2.78e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000203254
AA Change: T9P

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)
SMART Domains Protein: ENSMUSP00000145254
Gene: ENSMUSG00000051586
AA Change: T9P

DomainStartEndE-ValueType
low complexity region 60 69 N/A INTRINSIC
Pfam:FAD_binding_3 86 142 8.6e-6 PFAM
low complexity region 162 175 N/A INTRINSIC
CH 520 619 2.2e-19 SMART
low complexity region 640 655 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000204248
AA Change: T9P

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)
SMART Domains Protein: ENSMUSP00000145439
Gene: ENSMUSG00000051586
AA Change: T9P

DomainStartEndE-ValueType
low complexity region 60 69 N/A INTRINSIC
Pfam:FAD_binding_3 86 142 9.1e-6 PFAM
low complexity region 162 175 N/A INTRINSIC
CH 520 619 2.2e-19 SMART
low complexity region 625 635 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204994
Predicted Effect probably benign
Transcript: ENSMUST00000207889
AA Change: T9P

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)
Predicted Effect probably benign
Transcript: ENSMUST00000207968
AA Change: T9P

PolyPhen 2 Score 0.059 (Sensitivity: 0.94; Specificity: 0.84)
Predicted Effect probably benign
Transcript: ENSMUST00000207993
AA Change: T9P

PolyPhen 2 Score 0.059 (Sensitivity: 0.94; Specificity: 0.84)
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 97.4%
  • 3x: 96.9%
  • 10x: 95.2%
  • 20x: 91.9%
Validation Efficiency 95% (90/95)
Allele List at MGI
Other mutations in this stock
Total: 88 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca6 A T 11: 110,110,144 (GRCm39) S600T probably benign Het
Acly T A 11: 100,386,717 (GRCm39) Q615L probably benign Het
Adgrf1 C A 17: 43,620,924 (GRCm39) T387K probably benign Het
Afg3l1 G T 8: 124,228,670 (GRCm39) K745N probably damaging Het
Armc8 T C 9: 99,418,312 (GRCm39) T39A possibly damaging Het
Atm C A 9: 53,403,533 (GRCm39) probably benign Het
Babam2 A G 5: 32,214,890 (GRCm39) T324A probably damaging Het
Btd C A 14: 31,384,246 (GRCm39) D77E possibly damaging Het
Cadm1 T A 9: 47,740,668 (GRCm39) probably benign Het
Card11 A T 5: 140,871,315 (GRCm39) D729E probably benign Het
Ccni G T 5: 93,335,967 (GRCm39) T144K possibly damaging Het
Ceacam23 C T 7: 17,607,255 (GRCm39) noncoding transcript Het
Cecr2 A G 6: 120,708,228 (GRCm39) T77A probably damaging Het
Cgas A G 9: 78,341,593 (GRCm39) probably null Het
Cpsf7 T C 19: 10,512,803 (GRCm39) F296L possibly damaging Het
Cyfip1 C A 7: 55,523,196 (GRCm39) N70K possibly damaging Het
Cyp4a12b A G 4: 115,271,259 (GRCm39) probably benign Het
Ddx20 A T 3: 105,585,896 (GRCm39) Y816* probably null Het
Ddx59 A G 1: 136,360,245 (GRCm39) I420V probably damaging Het
Dgat1 T A 15: 76,386,703 (GRCm39) M445L probably damaging Het
Dnah5 T A 15: 28,246,546 (GRCm39) L628* probably null Het
Dnah7a A C 1: 53,598,307 (GRCm39) D1409E probably benign Het
Dnmt1 T C 9: 20,838,422 (GRCm39) T215A probably benign Het
Dsg4 C T 18: 20,604,302 (GRCm39) T923M probably damaging Het
Enox1 A G 14: 77,852,915 (GRCm39) I394V possibly damaging Het
Esrp2 A T 8: 106,861,250 (GRCm39) M183K probably damaging Het
Fam171a1 C T 2: 3,179,410 (GRCm39) P79S probably benign Het
Fga A G 3: 82,939,082 (GRCm39) T486A probably benign Het
Fkbp10 G T 11: 100,306,715 (GRCm39) A36S probably benign Het
Fnip1 A G 11: 54,393,279 (GRCm39) T572A probably benign Het
Fxn A C 19: 24,257,765 (GRCm39) probably null Het
Gaa C T 11: 119,175,324 (GRCm39) Q901* probably null Het
Gabrg1 A G 5: 70,911,594 (GRCm39) M344T probably benign Het
Galnt7 C T 8: 57,991,212 (GRCm39) V433M probably damaging Het
Gin1 A G 1: 97,712,951 (GRCm39) probably null Het
Hydin A C 8: 111,259,459 (GRCm39) D2477A probably benign Het
Igsf6 T C 7: 120,670,031 (GRCm39) Y37C probably damaging Het
Il18rap A G 1: 40,570,687 (GRCm39) I210V probably benign Het
Kif3a T C 11: 53,489,561 (GRCm39) Y138H probably damaging Het
Klra17 A G 6: 129,845,681 (GRCm39) probably null Het
Lcorl A T 5: 45,952,688 (GRCm39) I55N probably damaging Het
Lrrc49 A G 9: 60,510,059 (GRCm39) S398P possibly damaging Het
Ltv1 A G 10: 13,055,018 (GRCm39) L384S probably damaging Het
Mageb3 A T 2: 121,784,918 (GRCm39) Y261* probably null Het
Myrip C T 9: 120,217,228 (GRCm39) S49L probably damaging Het
Nrap T C 19: 56,372,487 (GRCm39) probably benign Het
Or2m12 T C 16: 19,104,627 (GRCm39) N289D probably damaging Het
Or4k5 A G 14: 50,385,728 (GRCm39) V201A probably benign Het
Otoa A T 7: 120,759,753 (GRCm39) probably benign Het
Pals2 T G 6: 50,140,411 (GRCm39) F144V probably benign Het
Parp11 T A 6: 127,467,008 (GRCm39) I133N probably damaging Het
Pcnx1 A G 12: 81,965,416 (GRCm39) T528A probably benign Het
Pde4c A G 8: 71,179,638 (GRCm39) H63R probably benign Het
Pdpk1 T C 17: 24,329,878 (GRCm39) K53E probably damaging Het
Pdzd7 A T 19: 45,024,615 (GRCm39) M468K probably damaging Het
Perm1 C A 4: 156,303,061 (GRCm39) P535Q possibly damaging Het
Pgap1 C A 1: 54,575,128 (GRCm39) A265S probably benign Het
Pik3c2a A T 7: 115,975,747 (GRCm39) probably null Het
Plxnd1 T C 6: 115,957,562 (GRCm39) T491A possibly damaging Het
Pms1 A T 1: 53,246,128 (GRCm39) D470E probably benign Het
Prf1 C A 10: 61,138,762 (GRCm39) T240N probably damaging Het
Prune2 A G 19: 17,099,445 (GRCm39) T1650A probably benign Het
Ptprf A G 4: 118,080,462 (GRCm39) L1264P probably benign Het
Ptprs A G 17: 56,726,527 (GRCm39) S948P probably damaging Het
Rapgef1 T C 2: 29,576,268 (GRCm39) V117A probably damaging Het
Rnf123 A G 9: 107,940,125 (GRCm39) V756A probably benign Het
Sez6l2 A G 7: 126,566,291 (GRCm39) E741G probably damaging Het
Shc3 A T 13: 51,626,888 (GRCm39) I125K possibly damaging Het
Smr3a A T 5: 88,155,917 (GRCm39) probably benign Het
Spef2 C T 15: 9,584,194 (GRCm39) E1624K probably damaging Het
St6gal1 A T 16: 23,140,083 (GRCm39) K85* probably null Het
Taf1b T G 12: 24,597,121 (GRCm39) D353E possibly damaging Het
Tcp10b C T 17: 13,286,590 (GRCm39) P180S possibly damaging Het
Tlr9 A T 9: 106,102,142 (GRCm39) M478L probably benign Het
Tpr A T 1: 150,295,654 (GRCm39) E892D probably damaging Het
Trio C T 15: 27,742,581 (GRCm39) W22* probably null Het
Usp14 A G 18: 10,024,673 (GRCm39) V8A probably damaging Het
Vmn2r19 A T 6: 123,307,011 (GRCm39) K506N possibly damaging Het
Vmn2r45 T A 7: 8,475,372 (GRCm39) N552I probably damaging Het
Vmn2r56 A T 7: 12,449,542 (GRCm39) M232K probably benign Het
Vps13b T A 15: 35,910,788 (GRCm39) F3517L possibly damaging Het
Yif1a C T 19: 5,142,333 (GRCm39) R247* probably null Het
Zbtb5 A G 4: 44,993,767 (GRCm39) V539A probably benign Het
Zfp180 G A 7: 23,804,652 (GRCm39) R357Q probably damaging Het
Zfp536 A G 7: 37,268,042 (GRCm39) L458P probably damaging Het
Zfp646 G A 7: 127,482,292 (GRCm39) G1490S probably benign Het
Zfp970 C T 2: 177,167,976 (GRCm39) H517Y probably damaging Het
Zranb3 A T 1: 127,945,293 (GRCm39) probably null Het
Other mutations in Mical3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00569:Mical3 APN 6 120,938,585 (GRCm39) missense possibly damaging 0.73
IGL00718:Mical3 APN 6 121,017,410 (GRCm39) missense probably damaging 0.98
IGL00940:Mical3 APN 6 120,999,371 (GRCm39) missense possibly damaging 0.55
IGL00973:Mical3 APN 6 120,911,885 (GRCm39) splice site probably benign
IGL01503:Mical3 APN 6 120,935,537 (GRCm39) missense probably benign 0.09
IGL01991:Mical3 APN 6 120,912,172 (GRCm39) missense probably damaging 0.98
IGL02794:Mical3 APN 6 120,984,270 (GRCm39) missense probably damaging 0.99
IGL02996:Mical3 APN 6 120,935,519 (GRCm39) missense probably damaging 1.00
IGL03105:Mical3 APN 6 121,019,199 (GRCm39) missense probably benign 0.01
IGL03109:Mical3 APN 6 120,986,085 (GRCm39) missense probably damaging 1.00
IGL03236:Mical3 APN 6 120,946,345 (GRCm39) missense probably benign 0.00
P0028:Mical3 UTSW 6 121,001,650 (GRCm39) missense probably benign 0.33
R0244:Mical3 UTSW 6 120,934,683 (GRCm39) missense probably benign 0.00
R0494:Mical3 UTSW 6 120,936,162 (GRCm39) missense possibly damaging 0.94
R0586:Mical3 UTSW 6 121,006,602 (GRCm39) unclassified probably benign
R1029:Mical3 UTSW 6 120,911,639 (GRCm39) missense probably benign 0.02
R1263:Mical3 UTSW 6 120,929,430 (GRCm39) missense probably damaging 0.99
R1507:Mical3 UTSW 6 121,019,199 (GRCm39) missense probably benign 0.36
R1527:Mical3 UTSW 6 121,001,740 (GRCm39) missense probably damaging 0.99
R1623:Mical3 UTSW 6 121,001,768 (GRCm39) missense probably damaging 0.99
R1680:Mical3 UTSW 6 120,936,604 (GRCm39) missense probably benign 0.09
R1697:Mical3 UTSW 6 120,984,369 (GRCm39) missense possibly damaging 0.84
R1875:Mical3 UTSW 6 121,019,025 (GRCm39) missense probably damaging 1.00
R1961:Mical3 UTSW 6 120,959,568 (GRCm39) missense possibly damaging 0.94
R2004:Mical3 UTSW 6 120,928,283 (GRCm39) missense probably damaging 1.00
R2093:Mical3 UTSW 6 121,017,347 (GRCm39) missense probably damaging 1.00
R2141:Mical3 UTSW 6 121,008,095 (GRCm39) splice site probably null
R2142:Mical3 UTSW 6 121,008,095 (GRCm39) splice site probably null
R2257:Mical3 UTSW 6 121,010,696 (GRCm39) missense possibly damaging 0.94
R2404:Mical3 UTSW 6 120,936,789 (GRCm39) missense probably benign 0.01
R2419:Mical3 UTSW 6 120,936,884 (GRCm39) missense probably benign
R2509:Mical3 UTSW 6 121,011,118 (GRCm39) missense probably damaging 1.00
R3784:Mical3 UTSW 6 120,998,298 (GRCm39) missense probably benign 0.00
R4342:Mical3 UTSW 6 120,911,799 (GRCm39) nonsense probably null
R4343:Mical3 UTSW 6 120,911,799 (GRCm39) nonsense probably null
R4579:Mical3 UTSW 6 120,935,660 (GRCm39) missense probably benign
R4603:Mical3 UTSW 6 120,911,799 (GRCm39) nonsense probably null
R4605:Mical3 UTSW 6 121,011,041 (GRCm39) nonsense probably null
R4610:Mical3 UTSW 6 120,911,799 (GRCm39) nonsense probably null
R4611:Mical3 UTSW 6 120,911,799 (GRCm39) nonsense probably null
R4623:Mical3 UTSW 6 120,938,586 (GRCm39) nonsense probably null
R4669:Mical3 UTSW 6 120,934,664 (GRCm39) missense probably damaging 0.98
R4704:Mical3 UTSW 6 120,935,649 (GRCm39) missense probably benign 0.00
R4722:Mical3 UTSW 6 121,015,486 (GRCm39) missense probably benign 0.00
R4863:Mical3 UTSW 6 121,010,748 (GRCm39) missense probably damaging 0.99
R4878:Mical3 UTSW 6 120,946,348 (GRCm39) missense possibly damaging 0.51
R4885:Mical3 UTSW 6 120,912,214 (GRCm39) missense probably damaging 1.00
R4907:Mical3 UTSW 6 120,984,259 (GRCm39) missense probably benign 0.00
R5007:Mical3 UTSW 6 121,015,030 (GRCm39) missense probably damaging 0.98
R5299:Mical3 UTSW 6 120,936,473 (GRCm39) missense possibly damaging 0.71
R5303:Mical3 UTSW 6 120,936,941 (GRCm39) missense probably benign
R5368:Mical3 UTSW 6 120,936,434 (GRCm39) missense probably damaging 1.00
R5955:Mical3 UTSW 6 121,010,711 (GRCm39) missense probably damaging 0.99
R5970:Mical3 UTSW 6 120,935,232 (GRCm39) nonsense probably null
R6000:Mical3 UTSW 6 120,998,281 (GRCm39) missense probably benign 0.06
R6101:Mical3 UTSW 6 121,010,671 (GRCm39) missense probably damaging 1.00
R6195:Mical3 UTSW 6 120,993,796 (GRCm39) intron probably benign
R6210:Mical3 UTSW 6 121,017,478 (GRCm39) splice site probably null
R6225:Mical3 UTSW 6 120,935,684 (GRCm39) missense probably damaging 0.98
R6258:Mical3 UTSW 6 120,985,991 (GRCm39) missense probably damaging 1.00
R6260:Mical3 UTSW 6 120,985,991 (GRCm39) missense probably damaging 1.00
R6349:Mical3 UTSW 6 120,936,486 (GRCm39) missense probably benign
R6352:Mical3 UTSW 6 120,929,434 (GRCm39) missense probably damaging 0.97
R6480:Mical3 UTSW 6 121,011,236 (GRCm39) missense possibly damaging 0.76
R6704:Mical3 UTSW 6 120,986,761 (GRCm39) intron probably benign
R6783:Mical3 UTSW 6 120,935,786 (GRCm39) missense possibly damaging 0.85
R6925:Mical3 UTSW 6 120,936,351 (GRCm39) missense probably benign 0.05
R6960:Mical3 UTSW 6 120,935,504 (GRCm39) missense probably damaging 1.00
R7170:Mical3 UTSW 6 120,950,694 (GRCm39) splice site probably null
R7344:Mical3 UTSW 6 121,013,505 (GRCm39) nonsense probably null
R7414:Mical3 UTSW 6 121,011,074 (GRCm39) missense probably damaging 1.00
R7455:Mical3 UTSW 6 120,935,705 (GRCm39) missense probably damaging 1.00
R7649:Mical3 UTSW 6 120,911,909 (GRCm39) missense probably damaging 1.00
R8236:Mical3 UTSW 6 120,989,504 (GRCm39) missense
R8286:Mical3 UTSW 6 120,998,149 (GRCm39) missense possibly damaging 0.68
R8316:Mical3 UTSW 6 120,911,944 (GRCm39) missense probably damaging 1.00
R8328:Mical3 UTSW 6 120,912,138 (GRCm39) missense probably damaging 0.98
R8354:Mical3 UTSW 6 120,950,381 (GRCm39) missense probably damaging 0.99
R8511:Mical3 UTSW 6 121,015,513 (GRCm39) missense possibly damaging 0.78
R8687:Mical3 UTSW 6 120,936,438 (GRCm39) missense probably benign 0.19
R8728:Mical3 UTSW 6 120,950,514 (GRCm39) missense probably damaging 0.99
R8925:Mical3 UTSW 6 120,984,325 (GRCm39) missense probably benign 0.00
R8927:Mical3 UTSW 6 120,984,325 (GRCm39) missense probably benign 0.00
R8986:Mical3 UTSW 6 120,991,822 (GRCm39) missense
R9026:Mical3 UTSW 6 120,986,848 (GRCm39) splice site probably benign
R9415:Mical3 UTSW 6 120,934,712 (GRCm39) missense probably damaging 1.00
R9515:Mical3 UTSW 6 121,001,758 (GRCm39) missense probably damaging 1.00
R9720:Mical3 UTSW 6 120,935,238 (GRCm39) missense probably damaging 0.99
R9777:Mical3 UTSW 6 120,959,529 (GRCm39) missense possibly damaging 0.91
U24488:Mical3 UTSW 6 120,978,457 (GRCm39) missense possibly damaging 0.90
Z1177:Mical3 UTSW 6 120,936,689 (GRCm39) missense possibly damaging 0.71
Z1190:Mical3 UTSW 6 120,998,319 (GRCm39) critical splice acceptor site probably null
Predicted Primers PCR Primer
(F):5'- TTGTCCAATTTGGCCCAGAG -3'
(R):5'- TTGAGTGCACACCTGTCAGG -3'

Sequencing Primer
(F):5'- GTCACAGAGCTCTTGGAA -3'
(R):5'- ACACCTGTCAGGGATGTGACTG -3'
Posted On 2014-06-23