Mutagenetix > Incidental Mutation

Incidental Mutation 'R1832:Ralgapa1'

204886

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, Tulip1, 2310003F20Rik

MMRRC Submission

039859-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.814) question?

Stock #

R1832 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

55649681-55867952 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 55804752 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 515 (T515A)

Ref Sequence

ENSEMBL: ENSMUSP00000154749 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably benign
Transcript: ENSMUST00000085385
AA Change: T515A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: T515A

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000110687
AA Change: T515A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: T515A

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000219432
AA Change: T515A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219529

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219542

Predicted Effect

probably benign
Transcript: ENSMUST00000220367
AA Change: T515A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000226244
AA Change: T515A

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)

Meta Mutation Damage Score

0.0697

Coding Region Coverage

1x: 97.4%
3x: 96.7%
10x: 94.6%
20x: 90.7%

Validation Efficiency

100% (82/82)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003H04Rik	T	A	3: 124,350,509 (GRCm39)	D143V	unknown	Het
Abca8a	A	T	11: 109,962,277 (GRCm39)	N525K	probably damaging	Het
Abhd12	T	A	2: 150,690,338 (GRCm39)	D119V	probably damaging	Het
Adamts20	A	T	15: 94,184,225 (GRCm39)	M1526K	probably benign	Het
Ankdd1a	A	T	9: 65,411,771 (GRCm39)		probably null	Het
Ankrd1	C	T	19: 36,092,378 (GRCm39)	C283Y	possibly damaging	Het
Arfgef1	G	C	1: 10,275,115 (GRCm39)	I312M	probably benign	Het
Bhlhe22	G	A	3: 18,109,139 (GRCm39)	C63Y	probably damaging	Het
Bmp8a	A	T	4: 123,218,885 (GRCm39)		probably benign	Het
Ccdc148	A	T	2: 58,891,911 (GRCm39)	S235T	probably damaging	Het
Ccdc88b	G	A	19: 6,830,900 (GRCm39)	Q681*	probably null	Het
Cep104	T	A	4: 154,087,003 (GRCm39)	V842E	probably benign	Het
Chac2	T	C	11: 30,927,568 (GRCm39)	N117S	probably benign	Het
Cimap3	C	T	3: 105,921,912 (GRCm39)	E4K	possibly damaging	Het
Cldn8	T	A	16: 88,359,746 (GRCm39)	I60F	probably benign	Het
Col16a1	G	A	4: 129,970,850 (GRCm39)		probably null	Het
Col4a1	A	G	8: 11,264,644 (GRCm39)		probably benign	Het
Cyp2a4	G	T	7: 26,011,635 (GRCm39)	E285D	probably damaging	Het
Cyp4a31	G	A	4: 115,426,928 (GRCm39)	G176D	probably benign	Het
Dmxl2	A	C	9: 54,368,233 (GRCm39)	Y246D	probably damaging	Het
Dync1h1	A	G	12: 110,580,493 (GRCm39)	K118R	probably damaging	Het
Dync2i1	T	A	12: 116,171,363 (GRCm39)	S958C	probably damaging	Het
Eif3h	T	C	15: 51,728,832 (GRCm39)	T8A	possibly damaging	Het
Fbh1	G	T	2: 11,772,211 (GRCm39)	L157I	probably benign	Het
Fbxo40	C	A	16: 36,789,218 (GRCm39)	G631*	probably null	Het
Gabrb1	T	A	5: 72,279,281 (GRCm39)		probably null	Het
Galc	A	G	12: 98,200,499 (GRCm39)		probably null	Het
Garin2	G	A	12: 78,762,280 (GRCm39)		probably benign	Het
H2-Q7	C	A	17: 35,658,675 (GRCm39)	S104R	probably benign	Het
Igkv13-54-1	A	T	6: 69,594,277 (GRCm39)	M31L	probably benign	Het
Lamc2	C	T	1: 153,041,933 (GRCm39)	R67Q	possibly damaging	Het
Lcn10	A	G	2: 25,575,151 (GRCm39)	D173G	probably damaging	Het
Llgl2	G	T	11: 115,741,926 (GRCm39)	R656L	probably damaging	Het
Lonrf2	G	A	1: 38,852,357 (GRCm39)	P165S	probably benign	Het
Lrrc66	G	A	5: 73,764,769 (GRCm39)	S758L	possibly damaging	Het
Ly6d	T	C	15: 74,634,615 (GRCm39)	K46E	probably damaging	Het
Map3k5	A	G	10: 19,975,306 (GRCm39)	N88D	probably damaging	Het
Mertk	A	T	2: 128,604,132 (GRCm39)	E422V	probably benign	Het
Mixl1	A	G	1: 180,522,296 (GRCm39)	V195A	probably benign	Het
Nmnat3	T	C	9: 98,281,521 (GRCm39)	V41A	probably damaging	Het
Or1r1	A	T	11: 73,875,319 (GRCm39)	N38K	probably damaging	Het
Or2a7	A	G	6: 43,151,834 (GRCm39)	R305G	probably benign	Het
Or7g34	A	G	9: 19,478,492 (GRCm39)	Y63H	possibly damaging	Het
Pappa2	T	A	1: 158,684,886 (GRCm39)	E751V	probably damaging	Het
Pcsk2	A	G	2: 143,635,189 (GRCm39)	S355G	probably damaging	Het
Pdzd2	A	G	15: 12,390,134 (GRCm39)	V821A	probably damaging	Het
Plxna4	A	C	6: 32,174,761 (GRCm39)	D1109E	probably benign	Het
Ppard	A	G	17: 28,516,084 (GRCm39)	M103V	probably benign	Het
Pramel51	T	C	12: 88,145,218 (GRCm39)	E44G	possibly damaging	Het
Rin2	A	G	2: 145,703,091 (GRCm39)	I596V	possibly damaging	Het
Rnls	A	G	19: 33,145,895 (GRCm39)	S75P	possibly damaging	Het
Rsph10b	A	T	5: 143,903,997 (GRCm39)	Y236F	possibly damaging	Het
Runx1t1	C	T	4: 13,835,628 (GRCm39)		probably benign	Het
Sardh	A	G	2: 27,125,581 (GRCm39)	V311A	possibly damaging	Het
Sbno2	G	T	10: 79,896,439 (GRCm39)	Y889*	probably null	Het
Sclt1	A	G	3: 41,681,546 (GRCm39)	V91A	probably damaging	Het
Sema4g	T	A	19: 44,987,456 (GRCm39)	V534E	probably benign	Het
Shoc1	T	G	4: 59,066,441 (GRCm39)	I768L	probably benign	Het
Slc10a1	G	A	12: 81,000,446 (GRCm39)	S351F	probably benign	Het
Slc19a3	A	T	1: 83,000,468 (GRCm39)	V183E	probably damaging	Het
Slc25a12	A	G	2: 71,164,054 (GRCm39)	Y74H	possibly damaging	Het
Slc6a19	T	A	13: 73,841,069 (GRCm39)	I114L	probably benign	Het
Smpd2	A	T	10: 41,364,232 (GRCm39)	C189S	probably benign	Het
Spon1	T	A	7: 113,616,018 (GRCm39)	V295D	probably benign	Het
Tet3	A	G	6: 83,380,627 (GRCm39)	S514P	probably benign	Het
Tnk1	T	C	11: 69,747,754 (GRCm39)	I49M	probably damaging	Het
Trim80	A	G	11: 115,337,619 (GRCm39)	T431A	probably benign	Het
Vgf	A	T	5: 137,060,153 (GRCm39)	Q105L	possibly damaging	Het
Vmn1r37	G	T	6: 66,708,780 (GRCm39)	L135F	probably benign	Het
Vps37d	C	T	5: 135,102,594 (GRCm39)	A128T	possibly damaging	Het
Wwp1	T	C	4: 19,650,197 (GRCm39)	D323G	probably benign	Het
Zfp456	T	A	13: 67,515,482 (GRCm39)	I75L	probably benign	Het
Zfp990	A	G	4: 145,264,780 (GRCm39)	I593V	possibly damaging	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,769,558 (GRCm39)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,793,970 (GRCm39)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,749,237 (GRCm39)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,756,360 (GRCm39)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,689,144 (GRCm39)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,689,133 (GRCm39)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,824,101 (GRCm39)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,766,442 (GRCm39)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,689,262 (GRCm39)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,754,862 (GRCm39)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,689,234 (GRCm39)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,759,450 (GRCm39)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,720,292 (GRCm39)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,723,202 (GRCm39)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,763,854 (GRCm39)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,804,736 (GRCm39)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
bantam	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
wickedwarlock	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,842,438 (GRCm39)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,741,942 (GRCm39)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,833,048 (GRCm39)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,786,290 (GRCm39)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,723,354 (GRCm39)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,723,823 (GRCm39)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,754,852 (GRCm39)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,736,576 (GRCm39)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,723,550 (GRCm39)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,829,670 (GRCm39)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,842,483 (GRCm39)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,712,448 (GRCm39)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,723,366 (GRCm39)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,809,446 (GRCm39)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,754,763 (GRCm39)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,723,711 (GRCm39)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,788,265 (GRCm39)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,731,309 (GRCm39)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,817,488 (GRCm39)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,788,321 (GRCm39)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,809,388 (GRCm39)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,723,552 (GRCm39)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,689,174 (GRCm39)	missense	probably damaging	1.00
R1870:Ralgapa1	UTSW	12	55,723,817 (GRCm39)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,833,107 (GRCm39)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,723,811 (GRCm39)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,741,945 (GRCm39)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,822,973 (GRCm39)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,723,909 (GRCm39)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,867,540 (GRCm39)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,705,922 (GRCm39)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,817,398 (GRCm39)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,741,928 (GRCm39)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,705,915 (GRCm39)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,745,552 (GRCm39)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,842,486 (GRCm39)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,786,115 (GRCm39)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,724,061 (GRCm39)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,740,243 (GRCm39)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,759,533 (GRCm39)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,841,778 (GRCm39)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,723,222 (GRCm39)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,745,588 (GRCm39)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,809,359 (GRCm39)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,764,899 (GRCm39)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,659,508 (GRCm39)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,712,459 (GRCm39)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,822,937 (GRCm39)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,804,817 (GRCm39)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,723,582 (GRCm39)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,766,408 (GRCm39)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,723,495 (GRCm39)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,659,523 (GRCm39)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,867,551 (GRCm39)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,723,898 (GRCm39)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,785,050 (GRCm39)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,817,401 (GRCm39)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,730,827 (GRCm39)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,804,709 (GRCm39)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,793,931 (GRCm39)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,730,695 (GRCm39)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,766,446 (GRCm39)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,785,104 (GRCm39)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,809,512 (GRCm39)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,651,058 (GRCm39)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,832,997 (GRCm39)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,822,976 (GRCm39)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,804,844 (GRCm39)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,867,508 (GRCm39)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,768,361 (GRCm39)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,650,976 (GRCm39)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,741,978 (GRCm39)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,755,789 (GRCm39)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,759,457 (GRCm39)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,765,013 (GRCm39)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,705,928 (GRCm39)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,756,341 (GRCm39)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,756,340 (GRCm39)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,788,298 (GRCm39)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,804,740 (GRCm39)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,788,304 (GRCm39)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,766,413 (GRCm39)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,659,423 (GRCm39)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,793,934 (GRCm39)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,744,663 (GRCm39)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,749,242 (GRCm39)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,829,631 (GRCm39)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,769,699 (GRCm39)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,788,308 (GRCm39)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,705,847 (GRCm39)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,723,303 (GRCm39)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,786,198 (GRCm39)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,785,002 (GRCm39)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,785,101 (GRCm39)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,749,345 (GRCm39)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,867,546 (GRCm39)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,723,791 (GRCm39)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,652,314 (GRCm39)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,723,351 (GRCm39)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,781,881 (GRCm39)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,755,843 (GRCm39)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,754,808 (GRCm39)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,659,485 (GRCm39)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,755,865 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGAATCCCAAAGTATAGGCATTTG -3'
(R):5'- AACAGTTTAGTCGAGTGGTCTAACC -3'

Sequencing Primer
(F):5'- TTTCTCTTGAAAGGTGGCACAC -3'
(R):5'- CTAACCATCTTAGAGACTCTTTATGC -3'

Posted On

2014-06-23