Mutagenetix > Incidental Mutation

Incidental Mutation 'R1836:Pdgfra'

205276

Institutional Source

Beutler Lab

Gene Symbol

Pdgfra

Ensembl Gene

ENSMUSG00000029231

Gene Name

platelet derived growth factor receptor, alpha polypeptide

Synonyms

Pdgfr-2, CD140a

MMRRC Submission

039863-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1836 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

75312953-75358876 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 75343675 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 732 (M732K)

Ref Sequence

ENSEMBL: ENSMUSP00000143906 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000476] [ENSMUST00000168162] [ENSMUST00000201711] [ENSMUST00000202681]

AlphaFold

P26618

Predicted Effect

possibly damaging
Transcript: ENSMUST00000000476
AA Change: M732K

PolyPhen 2 Score 0.954 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000000476
Gene: ENSMUSG00000029231
AA Change: M732K

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IG	34	122	6.07e-3	SMART
IG_like	135	206	1.7e1	SMART
IGc2	226	297	8.72e-4	SMART
IG	322	414	2.86e0	SMART
transmembrane domain	527	549	N/A	INTRINSIC
TyrKc	593	950	8.51e-141	SMART
Blast:TyrKc	960	991	3e-8	BLAST
low complexity region	1063	1082	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000168162
AA Change: M732K

PolyPhen 2 Score 0.954 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000127173
Gene: ENSMUSG00000029231
AA Change: M732K

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IG	34	122	6.07e-3	SMART
IG_like	135	206	1.7e1	SMART
IGc2	226	297	8.72e-4	SMART
IG	322	414	2.86e0	SMART
transmembrane domain	527	549	N/A	INTRINSIC
TyrKc	593	950	8.51e-141	SMART
Blast:TyrKc	960	991	3e-8	BLAST
low complexity region	1063	1082	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000201711
AA Change: M732K

PolyPhen 2 Score 0.954 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000143891
Gene: ENSMUSG00000029231
AA Change: M732K

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IG	34	122	6.07e-3	SMART
IG_like	135	206	1.7e1	SMART
IGc2	226	297	8.72e-4	SMART
IG	322	414	2.86e0	SMART
transmembrane domain	527	549	N/A	INTRINSIC
Pfam:Pkinase	593	701	9.9e-14	PFAM
Pfam:Pkinase_Tyr	593	750	5.2e-32	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000202681
AA Change: M732K

PolyPhen 2 Score 0.954 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000143906
Gene: ENSMUSG00000029231
AA Change: M732K

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IG	34	122	6.07e-3	SMART
IG_like	135	206	1.7e1	SMART
IGc2	226	297	8.72e-4	SMART
IG	322	414	2.86e0	SMART
transmembrane domain	527	549	N/A	INTRINSIC
Pfam:Pkinase	593	701	9.9e-14	PFAM
Pfam:Pkinase_Tyr	593	750	5.2e-32	PFAM

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.8%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the receptor tyrosine kinase family of proteins. Binding of platelet-derived growth factor protein ligands to this receptor triggers receptor dimerization and autophosphorylation, resulting in the activation of several downstream signaling pathways. Signaling through the encoded receptor plays a role in gastrulation and the development of nearly all organ systems. Mice lacking a functional copy of this gene reportedly exhibit defects in lung, skeleton, testis and the central nervous system, and die soon after birth. Alternative splicing and intronic polyadenylation of gene transcripts have been implicated in muscle regeneration and fibrosis in adult mice. [provided by RefSeq, Jan 2017]
PHENOTYPE: Homozygotes for targeted null mutations exhibit incomplete cephalic closure, increased apoptosis of neural crest cells, impaired myotome and testis formation, abnormal mucosal linings, thoracic skeletal defects, and midgestational lethality. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 108 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	A	G	13: 77,471,493 (GRCm39)	E966G	probably benign	Het
9330159F19Rik	T	C	10: 29,097,795 (GRCm39)	V64A	probably damaging	Het
Abcb5	G	T	12: 118,831,696 (GRCm39)	Q1219K	possibly damaging	Het
Acox2	A	T	14: 8,248,059 (GRCm38)	C408S	possibly damaging	Het
Acsf3	T	A	8: 123,506,922 (GRCm39)	Y72N	probably damaging	Het
Acss2	T	A	2: 155,400,550 (GRCm39)	Y530N	probably damaging	Het
Adam28	T	C	14: 68,886,870 (GRCm39)	E48G	possibly damaging	Het
Adgrv1	A	C	13: 81,652,232 (GRCm39)	M2957R	probably benign	Het
Adi1	C	A	12: 28,729,562 (GRCm39)	D138E	probably benign	Het
Alk	A	C	17: 72,198,032 (GRCm39)	L1228R	probably damaging	Het
Alms1	T	A	6: 85,655,485 (GRCm39)	S3344T	possibly damaging	Het
Aox1	G	A	1: 58,348,150 (GRCm39)	A623T	probably benign	Het
Arhgef2	C	T	3: 88,546,766 (GRCm39)	T545I	probably damaging	Het
Atp8a2	T	A	14: 60,243,815 (GRCm39)	N630I	possibly damaging	Het
Bcl9	G	T	3: 97,113,186 (GRCm39)	Q1090K	probably damaging	Het
Bdp1	A	G	13: 100,171,653 (GRCm39)	S2152P	probably benign	Het
Birc6	T	A	17: 74,921,385 (GRCm39)	S2155T	probably benign	Het
Camk2b	T	C	11: 5,922,384 (GRCm39)	E488G	probably damaging	Het
Capn9	T	A	8: 125,332,304 (GRCm39)		probably null	Het
Cd209f	A	G	8: 4,154,491 (GRCm39)	S119P	probably damaging	Het
Cep78	T	C	19: 15,946,533 (GRCm39)	E433G	probably damaging	Het
Chid1	G	T	7: 141,106,409 (GRCm39)		probably null	Het
Cldnd2	T	C	7: 43,092,349 (GRCm39)	S129P	possibly damaging	Het
Cobll1	A	T	2: 64,956,580 (GRCm39)	F289Y	probably damaging	Het
Creb1	C	T	1: 64,590,109 (GRCm39)	Q32*	probably null	Het
Creb3l4	G	T	3: 90,146,210 (GRCm39)	S182Y	probably benign	Het
Dgcr2	A	T	16: 17,667,584 (GRCm39)	C292S	probably damaging	Het
Dnah8	T	C	17: 31,093,901 (GRCm39)	V4665A	possibly damaging	Het
Dnah9	A	T	11: 66,009,667 (GRCm39)	M740K	probably benign	Het
Dnmt1	G	A	9: 20,829,542 (GRCm39)	T687I	probably damaging	Het
Ece1	C	T	4: 137,685,312 (GRCm39)	R601W	probably damaging	Het
Echdc2	C	A	4: 108,022,732 (GRCm39)	R3S	probably damaging	Het
Ep400	A	T	5: 110,852,920 (GRCm39)	L1275Q	unknown	Het
Epha6	A	T	16: 60,026,108 (GRCm39)	W445R	probably damaging	Het
Ercc5	T	A	1: 44,220,035 (GRCm39)	S1102R	probably benign	Het
Fam228a	T	G	12: 4,765,620 (GRCm39)	T264P	probably damaging	Het
Fzd6	T	A	15: 38,897,315 (GRCm39)	I488N	probably damaging	Het
Gabrg3	T	G	7: 56,379,389 (GRCm39)	N338H	probably damaging	Het
Gde1	A	G	7: 118,294,357 (GRCm39)	F39L	possibly damaging	Het
Gjb3	G	A	4: 127,220,224 (GRCm39)	R103W	probably damaging	Het
Gm4781	T	A	10: 100,232,582 (GRCm39)		noncoding transcript	Het
Gm4846	T	A	1: 166,311,492 (GRCm39)	T456S	probably benign	Het
Gpx5	A	G	13: 21,471,624 (GRCm39)	I193T	probably benign	Het
H3c2	T	A	13: 23,936,715 (GRCm39)	V118D	probably damaging	Het
Herc2	A	T	7: 55,804,853 (GRCm39)	K2294*	probably null	Het
Iqgap3	T	A	3: 88,015,675 (GRCm39)	V19D	probably damaging	Het
Itga5	T	C	15: 103,254,441 (GRCm39)	I1006V	probably damaging	Het
Lamb1	C	T	12: 31,351,093 (GRCm39)	T781I	probably benign	Het
Lias	A	G	5: 65,549,686 (GRCm39)	T57A	probably benign	Het
Lin37	C	A	7: 30,256,368 (GRCm39)	R108L	probably damaging	Het
Lrrd1	A	T	5: 3,915,709 (GRCm39)	T769S	probably benign	Het
Mamstr	T	C	7: 45,294,387 (GRCm39)	W414R	probably damaging	Het
Mtmr11	T	G	3: 96,072,103 (GRCm39)	S233R	probably damaging	Het
Myh1	A	T	11: 67,095,648 (GRCm39)	I266F	probably damaging	Het
Myt1	C	A	2: 181,439,068 (GRCm39)	Q197K	probably benign	Het
Naip5	G	T	13: 100,356,195 (GRCm39)	T1140K	probably benign	Het
Ncf2	G	A	1: 152,683,822 (GRCm39)	V14M	probably damaging	Het
Ndufb11b	T	C	15: 81,865,068 (GRCm39)	V103A	probably damaging	Het
Nhsl1	G	T	10: 18,400,653 (GRCm39)	R626S	possibly damaging	Het
Nol12	T	A	15: 78,822,089 (GRCm39)	V108E	probably damaging	Het
Nr1i2	G	T	16: 38,069,644 (GRCm39)	P420Q	probably damaging	Het
Nup155	A	G	15: 8,184,464 (GRCm39)	I1286M	possibly damaging	Het
Ocrl	T	A	X: 47,050,993 (GRCm39)	I74N	probably damaging	Het
Or4c10b	A	C	2: 89,711,629 (GRCm39)	H153P	probably damaging	Het
Or51b17	A	G	7: 103,542,592 (GRCm39)	S117P	probably damaging	Het
Or5ak23	A	G	2: 85,244,749 (GRCm39)	V158A	probably benign	Het
Pard3b	C	T	1: 62,676,763 (GRCm39)	S999L	probably benign	Het
Pax9	A	G	12: 56,746,839 (GRCm39)	E225G	probably benign	Het
Pdcd4	T	G	19: 53,914,650 (GRCm39)	L335R	probably damaging	Het
Pkhd1	G	T	1: 20,187,293 (GRCm39)	Q3672K	probably benign	Het
Pold2	A	G	11: 5,823,454 (GRCm39)	L325P	possibly damaging	Het
Pom121l2	A	T	13: 22,167,954 (GRCm39)	T742S	probably benign	Het
Ppfia1	C	T	7: 144,073,368 (GRCm39)	E208K	probably benign	Het
Prex2	A	T	1: 11,207,004 (GRCm39)	R521W	probably damaging	Het
Prr23a4	C	A	9: 98,785,535 (GRCm39)	R67S	probably benign	Het
Rhpn2	T	C	7: 35,071,813 (GRCm39)	L226P	probably benign	Het
Rps12	C	A	10: 23,661,527 (GRCm39)	D95Y	probably damaging	Het
Sardh	T	A	2: 27,105,194 (GRCm39)	D643V	possibly damaging	Het
Sars1	T	C	3: 108,343,260 (GRCm39)	D77G	probably benign	Het
Scin	C	A	12: 40,174,697 (GRCm39)	V129F	probably damaging	Het
Semp2l1	T	G	1: 32,584,758 (GRCm39)	D384A	probably damaging	Het
Sftpa1	T	A	14: 40,854,803 (GRCm39)	M66K	possibly damaging	Het
Sgo1	T	C	17: 53,994,799 (GRCm39)	K28E	probably damaging	Het
Sim2	G	T	16: 93,924,436 (GRCm39)		probably null	Het
Srp54b	T	A	12: 55,296,945 (GRCm39)		probably null	Het
Stard7	T	C	2: 127,137,480 (GRCm39)	V310A	probably benign	Het
Tasp1	T	C	2: 139,793,477 (GRCm39)	D233G	probably damaging	Het
Tdrd6	G	T	17: 43,936,480 (GRCm39)	L1523M	probably benign	Het
Tle3	A	G	9: 61,321,305 (GRCm39)	D639G	probably damaging	Het
Tmem128	A	G	5: 38,417,750 (GRCm39)	D2G	probably damaging	Het
Tmem163	A	G	1: 127,605,246 (GRCm39)	S41P	probably benign	Het
Ttn	C	T	2: 76,559,505 (GRCm39)	S29632N	probably damaging	Het
Tuba4a	A	G	1: 75,192,754 (GRCm39)	S287P	probably benign	Het
Ubn1	G	C	16: 4,895,255 (GRCm39)	G767A	probably benign	Het
Ugdh	A	T	5: 65,577,634 (GRCm39)	C288*	probably null	Het
Upf2	T	A	2: 6,055,135 (GRCm39)		probably null	Het
Vmn1r2	T	C	4: 3,172,836 (GRCm39)	S252P	probably damaging	Het
Vmn1r28	A	C	6: 58,242,237 (GRCm39)	T27P	probably damaging	Het
Vmn1r77	T	C	7: 11,775,338 (GRCm39)	I38T	probably benign	Het
Vmn2r51	C	A	7: 9,832,090 (GRCm39)	E499*	probably null	Het
Vmn2r51	G	T	7: 9,832,091 (GRCm39)	Y498*	probably null	Het
Vmn2r53	A	T	7: 12,334,812 (GRCm39)	W283R	probably damaging	Het
Vpreb1a	T	A	16: 16,686,933 (GRCm39)	T15S	probably benign	Het
Vps13b	T	C	15: 35,910,378 (GRCm39)	S3381P	probably damaging	Het
Wnt2	A	T	6: 18,023,234 (GRCm39)	D138E	probably damaging	Het
Zdhhc22	A	G	12: 87,030,204 (GRCm39)	V248A	probably benign	Het
Zfp30	T	A	7: 29,492,805 (GRCm39)	I434N	probably damaging	Het
Zfp316	A	G	5: 143,239,178 (GRCm39)	L947P	probably damaging	Het

Other mutations in Pdgfra

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00489:Pdgfra	APN	5	75,324,340 (GRCm39)	missense	probably benign	0.40
IGL00574:Pdgfra	APN	5	75,341,708 (GRCm39)	missense	probably damaging	1.00
IGL00906:Pdgfra	APN	5	75,340,834 (GRCm39)	missense	probably benign	0.00
IGL00964:Pdgfra	APN	5	75,335,726 (GRCm39)	missense	probably damaging	1.00
IGL01467:Pdgfra	APN	5	75,346,292 (GRCm39)	critical splice donor site	probably null
IGL01485:Pdgfra	APN	5	75,324,313 (GRCm39)	missense	probably benign	0.02
IGL01556:Pdgfra	APN	5	75,338,352 (GRCm39)	missense	probably damaging	1.00
IGL01949:Pdgfra	APN	5	75,331,326 (GRCm39)	missense	probably damaging	0.98
IGL02066:Pdgfra	APN	5	75,331,241 (GRCm39)	missense	possibly damaging	0.55
IGL02271:Pdgfra	APN	5	75,348,567 (GRCm39)	missense	probably damaging	1.00
IGL02726:Pdgfra	APN	5	75,355,618 (GRCm39)	nonsense	probably null
IGL02858:Pdgfra	APN	5	75,355,635 (GRCm39)	missense	probably damaging	1.00
IGL03306:Pdgfra	APN	5	75,353,194 (GRCm39)	missense	possibly damaging	0.49
Pony_express	UTSW	5	75,349,895 (GRCm39)	nonsense	probably null
P0033:Pdgfra	UTSW	5	75,353,222 (GRCm39)	missense	probably damaging	1.00
PIT4472001:Pdgfra	UTSW	5	75,340,907 (GRCm39)	missense	probably damaging	1.00
R0134:Pdgfra	UTSW	5	75,327,172 (GRCm39)	missense	probably damaging	1.00
R0200:Pdgfra	UTSW	5	75,324,438 (GRCm39)	missense	probably damaging	1.00
R0254:Pdgfra	UTSW	5	75,328,596 (GRCm39)	missense	probably damaging	1.00
R0331:Pdgfra	UTSW	5	75,355,713 (GRCm39)	missense	probably damaging	1.00
R0467:Pdgfra	UTSW	5	75,355,697 (GRCm39)	missense	probably damaging	1.00
R0532:Pdgfra	UTSW	5	75,331,434 (GRCm39)	missense	probably benign	0.00
R0608:Pdgfra	UTSW	5	75,324,438 (GRCm39)	missense	probably damaging	1.00
R0765:Pdgfra	UTSW	5	75,348,648 (GRCm39)	unclassified	probably benign
R1171:Pdgfra	UTSW	5	75,334,108 (GRCm39)	missense	probably damaging	0.98
R1372:Pdgfra	UTSW	5	75,349,924 (GRCm39)	missense	probably damaging	0.96
R1530:Pdgfra	UTSW	5	75,349,671 (GRCm39)	splice site	probably null
R1585:Pdgfra	UTSW	5	75,353,264 (GRCm39)	missense	probably damaging	1.00
R1666:Pdgfra	UTSW	5	75,349,681 (GRCm39)	missense	possibly damaging	0.94
R1868:Pdgfra	UTSW	5	75,331,534 (GRCm39)	missense	probably benign	0.43
R1923:Pdgfra	UTSW	5	75,324,394 (GRCm39)	missense	probably benign	0.03
R2075:Pdgfra	UTSW	5	75,348,609 (GRCm39)	missense	probably damaging	1.00
R2261:Pdgfra	UTSW	5	75,346,184 (GRCm39)	missense	probably benign	0.03
R2262:Pdgfra	UTSW	5	75,346,184 (GRCm39)	missense	probably benign	0.03
R3028:Pdgfra	UTSW	5	75,335,642 (GRCm39)	missense	probably damaging	1.00
R3236:Pdgfra	UTSW	5	75,328,597 (GRCm39)	missense	probably damaging	1.00
R3692:Pdgfra	UTSW	5	75,349,948 (GRCm39)	missense	possibly damaging	0.54
R3701:Pdgfra	UTSW	5	75,340,881 (GRCm39)	nonsense	probably null
R3890:Pdgfra	UTSW	5	75,328,588 (GRCm39)	missense	probably null	0.57
R3901:Pdgfra	UTSW	5	75,353,169 (GRCm39)	missense	probably benign	0.10
R3902:Pdgfra	UTSW	5	75,353,169 (GRCm39)	missense	probably benign	0.10
R4272:Pdgfra	UTSW	5	75,343,731 (GRCm39)	missense	probably benign	0.05
R4532:Pdgfra	UTSW	5	75,341,744 (GRCm39)	missense	probably damaging	1.00
R4660:Pdgfra	UTSW	5	75,322,932 (GRCm39)	missense	possibly damaging	0.82
R4753:Pdgfra	UTSW	5	75,342,185 (GRCm39)	missense	probably damaging	1.00
R4795:Pdgfra	UTSW	5	75,349,972 (GRCm39)	missense	probably benign
R4796:Pdgfra	UTSW	5	75,349,972 (GRCm39)	missense	probably benign
R4884:Pdgfra	UTSW	5	75,349,973 (GRCm39)	missense	probably benign	0.07
R4936:Pdgfra	UTSW	5	75,355,687 (GRCm39)	missense	probably damaging	1.00
R5625:Pdgfra	UTSW	5	75,349,998 (GRCm39)	critical splice donor site	probably null
R5666:Pdgfra	UTSW	5	75,334,156 (GRCm39)	missense	probably benign	0.00
R5670:Pdgfra	UTSW	5	75,334,156 (GRCm39)	missense	probably benign	0.00
R5714:Pdgfra	UTSW	5	75,346,673 (GRCm39)	missense	probably damaging	1.00
R5836:Pdgfra	UTSW	5	75,324,435 (GRCm39)	missense	possibly damaging	0.52
R6126:Pdgfra	UTSW	5	75,331,190 (GRCm39)	missense	probably benign	0.09
R6141:Pdgfra	UTSW	5	75,334,057 (GRCm39)	missense	probably damaging	0.98
R6297:Pdgfra	UTSW	5	75,334,135 (GRCm39)	missense	possibly damaging	0.88
R6363:Pdgfra	UTSW	5	75,331,497 (GRCm39)	missense	possibly damaging	0.91
R6376:Pdgfra	UTSW	5	75,327,180 (GRCm39)	missense	probably benign	0.02
R6485:Pdgfra	UTSW	5	75,335,735 (GRCm39)	splice site	probably null
R6612:Pdgfra	UTSW	5	75,328,503 (GRCm39)	missense	probably benign	0.01
R6641:Pdgfra	UTSW	5	75,322,762 (GRCm39)	intron	probably benign
R6954:Pdgfra	UTSW	5	75,334,055 (GRCm39)	missense	possibly damaging	0.82
R7110:Pdgfra	UTSW	5	75,349,895 (GRCm39)	nonsense	probably null
R7192:Pdgfra	UTSW	5	75,343,767 (GRCm39)	missense	probably damaging	1.00
R7294:Pdgfra	UTSW	5	75,342,312 (GRCm39)	missense	probably benign	0.05
R7347:Pdgfra	UTSW	5	75,343,759 (GRCm39)	missense	possibly damaging	0.91
R7476:Pdgfra	UTSW	5	75,331,264 (GRCm39)	missense	probably damaging	1.00
R7512:Pdgfra	UTSW	5	75,355,675 (GRCm39)	nonsense	probably null
R7609:Pdgfra	UTSW	5	75,327,382 (GRCm39)	missense	probably benign	0.10
R7925:Pdgfra	UTSW	5	75,353,079 (GRCm39)	splice site	probably benign
R8141:Pdgfra	UTSW	5	75,338,387 (GRCm39)	missense	possibly damaging	0.81
R8490:Pdgfra	UTSW	5	75,331,329 (GRCm39)	critical splice donor site	probably null
R8886:Pdgfra	UTSW	5	75,343,734 (GRCm39)	missense	probably benign	0.03
R9234:Pdgfra	UTSW	5	75,324,262 (GRCm39)	missense	possibly damaging	0.93
R9339:Pdgfra	UTSW	5	75,355,635 (GRCm39)	missense	probably damaging	1.00
R9459:Pdgfra	UTSW	5	75,353,129 (GRCm39)	missense	probably damaging	1.00
R9475:Pdgfra	UTSW	5	75,328,588 (GRCm39)	missense	possibly damaging	0.93
R9519:Pdgfra	UTSW	5	75,337,350 (GRCm39)	missense	probably benign	0.00
Z1088:Pdgfra	UTSW	5	75,327,238 (GRCm39)	missense	probably benign	0.03
Z1177:Pdgfra	UTSW	5	75,342,335 (GRCm39)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- TTGATACCTCTCTCAGTTAATGGCAC -3'
(R):5'- TGCACTTAGGGAGCACACTG -3'

Sequencing Primer
(F):5'- TGGCACACTCTAGGTACCCAAATTAG -3'
(R):5'- TGGCAGAGCTACCTGTCATC -3'

Posted On

2014-06-23