Mutagenetix > Incidental Mutation

Incidental Mutation 'R1856:Dnajc6'

206099

Institutional Source

Beutler Lab

Gene Symbol

Dnajc6

Ensembl Gene

ENSMUSG00000028528

Gene Name

DnaJ heat shock protein family (Hsp40) member C6

Synonyms

auxilin, 2810027M23Rik

MMRRC Submission

039880-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R1856 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

101353828-101499996 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 101456185 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 58 (S58P)

Ref Sequence

ENSEMBL: ENSMUSP00000114840 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038207] [ENSMUST00000094953] [ENSMUST00000106929] [ENSMUST00000106930] [ENSMUST00000106933] [ENSMUST00000149047] [ENSMUST00000154120]

AlphaFold

Q80TZ3

Predicted Effect

probably damaging
Transcript: ENSMUST00000038207
AA Change: S96P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000044251
Gene: ENSMUSG00000028528
AA Change: S96P

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	88	244	1e-20	SMART
PTEN_C2	251	390	5.95e-42	SMART
low complexity region	502	521	N/A	INTRINSIC
low complexity region	554	569	N/A	INTRINSIC
low complexity region	679	694	N/A	INTRINSIC
low complexity region	719	735	N/A	INTRINSIC
low complexity region	829	840	N/A	INTRINSIC
DnaJ	873	934	2e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000094953
AA Change: S58P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000092560
Gene: ENSMUSG00000028528
AA Change: S58P

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	50	206	2e-20	SMART
PTEN_C2	213	352	5.95e-42	SMART
low complexity region	464	483	N/A	INTRINSIC
low complexity region	516	531	N/A	INTRINSIC
low complexity region	641	656	N/A	INTRINSIC
low complexity region	681	697	N/A	INTRINSIC
low complexity region	791	802	N/A	INTRINSIC
DnaJ	835	896	2e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106929
AA Change: S58P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102542
Gene: ENSMUSG00000028528
AA Change: S58P

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	50	206	2e-20	SMART
PTEN_C2	213	352	5.95e-42	SMART
low complexity region	464	483	N/A	INTRINSIC
low complexity region	516	531	N/A	INTRINSIC
low complexity region	641	656	N/A	INTRINSIC
low complexity region	681	697	N/A	INTRINSIC
low complexity region	791	802	N/A	INTRINSIC
DnaJ	835	896	2e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106930
AA Change: S58P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102543
Gene: ENSMUSG00000028528
AA Change: S58P

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	50	206	2e-20	SMART
PTEN_C2	213	352	5.95e-42	SMART
low complexity region	464	483	N/A	INTRINSIC
low complexity region	516	531	N/A	INTRINSIC
low complexity region	641	656	N/A	INTRINSIC
low complexity region	681	697	N/A	INTRINSIC
low complexity region	791	802	N/A	INTRINSIC
DnaJ	835	896	2e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106933
AA Change: S126P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000102546
Gene: ENSMUSG00000028528
AA Change: S126P

Domain	Start	End	E-Value	Type
low complexity region	30	44	N/A	INTRINSIC
SCOP:d1d5ra2	118	274	1e-20	SMART
PTEN_C2	281	420	5.95e-42	SMART
low complexity region	532	551	N/A	INTRINSIC
low complexity region	584	599	N/A	INTRINSIC
low complexity region	709	724	N/A	INTRINSIC
low complexity region	749	765	N/A	INTRINSIC
low complexity region	859	870	N/A	INTRINSIC
DnaJ	903	964	2e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146489

Predicted Effect

probably damaging
Transcript: ENSMUST00000149047
AA Change: S58P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000119542
Gene: ENSMUSG00000028528
AA Change: S58P

Domain	Start	End	E-Value	Type
PDB:3N0A\|A	30	194	1e-118	PDB
SCOP:d1d5ra2	50	187	2e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000154120
AA Change: S58P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000114840
Gene: ENSMUSG00000028528
AA Change: S58P

Domain	Start	End	E-Value	Type
PDB:3N0A\|A	30	116	4e-54	PDB
SCOP:d1d5ra2	50	101	1e-4	SMART

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]
PHENOTYPE: Mice homozygous and heterozygous for a knock-out allele exhibit postnatal lethality and decreased body weight with homozygotes exhibiting decreased synpatic vesicle recycling. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm5	T	A	4: 144,504,453 (GRCm39)	M233L	probably benign	Het
Abca14	T	G	7: 119,877,404 (GRCm39)	F1017L	probably damaging	Het
Abcb11	A	C	2: 69,076,267 (GRCm39)	V1147G	probably damaging	Het
Abcc10	T	A	17: 46,617,529 (GRCm39)	S1128C	probably damaging	Het
Adam6a	G	A	12: 113,508,923 (GRCm39)	C432Y	probably damaging	Het
Adamdec1	C	T	14: 68,808,397 (GRCm39)	V318M	probably damaging	Het
Ahnak	C	A	19: 8,979,412 (GRCm39)	S232Y	possibly damaging	Het
Amt	A	C	9: 108,174,361 (GRCm39)	H42P	probably damaging	Het
Anapc1	A	T	2: 128,501,708 (GRCm39)	L778Q	probably damaging	Het
Ankhd1	G	A	18: 36,777,580 (GRCm39)	A1588T	probably benign	Het
Anln	A	T	9: 22,264,627 (GRCm39)	L881Q	probably damaging	Het
Appl1	A	G	14: 26,649,706 (GRCm39)	S607P	probably damaging	Het
Arg2	G	T	12: 79,194,436 (GRCm39)	V87L	probably benign	Het
Atp13a2	C	T	4: 140,731,323 (GRCm39)	P869L	probably benign	Het
Atxn7l1	T	A	12: 33,408,769 (GRCm39)	D310E	probably damaging	Het
Cd6	A	T	19: 10,775,966 (GRCm39)	D164E	probably damaging	Het
Cdan1	C	A	2: 120,555,417 (GRCm39)	V775L	probably benign	Het
Cep164	A	T	9: 45,687,056 (GRCm39)		probably null	Het
Cep41	A	G	6: 30,661,005 (GRCm39)	S61P	probably damaging	Het
Clcn7	C	A	17: 25,379,445 (GRCm39)	D764E	probably damaging	Het
Cog2	G	A	8: 125,278,142 (GRCm39)	G703S	possibly damaging	Het
Cramp1	T	C	17: 25,187,952 (GRCm39)	D1214G	probably damaging	Het
Cst7	G	T	2: 150,419,628 (GRCm39)	C98F	probably damaging	Het
Ctc1	T	A	11: 68,925,484 (GRCm39)	L1007Q	probably damaging	Het
Cyb5r4	G	A	9: 86,904,262 (GRCm39)	A11T	possibly damaging	Het
Dcaf8	A	G	1: 172,003,120 (GRCm39)	D306G	probably damaging	Het
Defb38	T	A	8: 19,073,592 (GRCm39)	K27I	probably benign	Het
Disp3	T	C	4: 148,356,089 (GRCm39)	E257G	probably damaging	Het
Dock10	A	T	1: 80,584,285 (GRCm39)	D140E	possibly damaging	Het
Dop1a	T	C	9: 86,374,057 (GRCm39)	V172A	probably damaging	Het
Dync1h1	G	A	12: 110,602,943 (GRCm39)	E2195K	probably benign	Het
Eci3	C	T	13: 35,137,011 (GRCm39)	A171T	possibly damaging	Het
Eml2	A	G	7: 18,927,986 (GRCm39)	D284G	probably damaging	Het
Eml5	T	C	12: 98,776,843 (GRCm39)	D1377G	probably damaging	Het
Fam186a	T	C	15: 99,838,183 (GRCm39)	E2687G	possibly damaging	Het
Fut9	A	C	4: 25,620,352 (GRCm39)	L154R	probably damaging	Het
Gabrb2	A	G	11: 42,517,540 (GRCm39)	N454S	probably benign	Het
Gpr183	T	A	14: 122,192,153 (GRCm39)	I123L	probably benign	Het
Gucy1b1	A	T	3: 81,965,659 (GRCm39)	N62K	probably benign	Het
Hcrtr2	T	C	9: 76,167,067 (GRCm39)	N90S	probably damaging	Het
Hectd1	A	T	12: 51,791,577 (GRCm39)	L2556Q	probably damaging	Het
Hfm1	T	A	5: 106,995,542 (GRCm39)	M1290L	probably benign	Het
Hgsnat	A	G	8: 26,447,284 (GRCm39)	W337R	probably benign	Het
Hmbox1	T	C	14: 65,066,097 (GRCm39)	Y291C	probably damaging	Het
Hmcn1	T	C	1: 150,597,415 (GRCm39)	N1749S	probably benign	Het
Igsf10	T	C	3: 59,238,693 (GRCm39)	D496G	possibly damaging	Het
Iqch	C	T	9: 63,441,619 (GRCm39)		probably null	Het
Irf6	A	G	1: 192,849,843 (GRCm39)	D255G	probably benign	Het
Kif9	G	A	9: 110,346,787 (GRCm39)	G642R	probably null	Het
Klhl20	A	G	1: 160,934,312 (GRCm39)	Y236H	probably benign	Het
Krt39	T	A	11: 99,409,914 (GRCm39)	K208*	probably null	Het
Lama3	T	A	18: 12,670,838 (GRCm39)	L808*	probably null	Het
Lrp5	C	T	19: 3,647,346 (GRCm39)	A1299T	probably benign	Het
Lysmd4	A	G	7: 66,875,979 (GRCm39)	Q214R	probably benign	Het
Macf1	T	A	4: 123,263,641 (GRCm39)	E6960V	probably damaging	Het
Mcpt2	A	G	14: 56,281,156 (GRCm39)	E120G	probably benign	Het
Mpeg1	A	G	19: 12,439,720 (GRCm39)	T393A	probably benign	Het
Myh4	G	A	11: 67,146,508 (GRCm39)	E1494K	probably damaging	Het
Nbas	T	G	12: 13,524,230 (GRCm39)	S1695R	possibly damaging	Het
Nlgn1	G	T	3: 25,494,201 (GRCm39)	Y249*	probably null	Het
Ntn4	C	T	10: 93,543,215 (GRCm39)	R314W	probably damaging	Het
Nup107	A	G	10: 117,586,811 (GRCm39)	L910P	probably damaging	Het
Obscn	T	A	11: 58,931,122 (GRCm39)	D5838V	probably damaging	Het
Or10g7	T	C	9: 39,905,655 (GRCm39)	I183T	probably benign	Het
Or1j18	T	C	2: 36,624,357 (GRCm39)	I8T	probably benign	Het
Or4c113	G	A	2: 88,885,203 (GRCm39)	T189M	possibly damaging	Het
Or8d2b	T	C	9: 38,788,892 (GRCm39)	I140T	possibly damaging	Het
Otogl	G	T	10: 107,690,125 (GRCm39)	S915Y	possibly damaging	Het
P2rx7	T	A	5: 122,819,095 (GRCm39)	C506S	probably damaging	Het
P4hb	T	C	11: 120,454,044 (GRCm39)	E350G	probably benign	Het
Papolg	A	T	11: 23,817,379 (GRCm39)	V606E	probably benign	Het
Pappa	A	T	4: 65,258,980 (GRCm39)	D1576V	probably damaging	Het
Pclo	G	T	5: 14,828,566 (GRCm39)	V1402F	probably damaging	Het
Pdcd11	A	G	19: 47,086,626 (GRCm39)	T211A	probably benign	Het
Pdzd2	T	C	15: 12,373,941 (GRCm39)	R2065G	possibly damaging	Het
Plcl2	T	C	17: 50,914,878 (GRCm39)	V629A	probably benign	Het
Prkcsh	G	A	9: 21,915,871 (GRCm39)	V92I	possibly damaging	Het
Prpf19	G	T	19: 10,879,780 (GRCm39)	V320F	probably damaging	Het
Ptpn3	T	A	4: 57,239,682 (GRCm39)	I283F	probably damaging	Het
Rcc2	T	A	4: 140,447,915 (GRCm39)	D480E	probably benign	Het
Rnf182	G	A	13: 43,821,518 (GRCm39)	C23Y	probably damaging	Het
Rnf186	C	T	4: 138,694,673 (GRCm39)	T71I	probably benign	Het
Scyl3	A	G	1: 163,761,265 (GRCm39)		probably null	Het
Slc12a6	G	T	2: 112,166,272 (GRCm39)		probably null	Het
Slc1a2	C	A	2: 102,607,912 (GRCm39)	S520Y	probably damaging	Het
Slc35e2	T	A	4: 155,696,186 (GRCm39)	L191Q	probably damaging	Het
Snrnp40	C	G	4: 130,271,836 (GRCm39)		probably null	Het
Snx33	T	C	9: 56,833,295 (GRCm39)	H258R	possibly damaging	Het
Tecpr2	A	T	12: 110,899,498 (GRCm39)	H622L	probably benign	Het
Thbs3	A	T	3: 89,133,713 (GRCm39)	E888D	probably damaging	Het
Tlk2	T	C	11: 105,112,124 (GRCm39)	L159P	probably benign	Het
Tmem70	A	T	1: 16,747,497 (GRCm39)	T205S	probably damaging	Het
Trappc10	T	A	10: 78,032,285 (GRCm39)	H1001L	probably benign	Het
Trim12a	T	C	7: 103,950,064 (GRCm39)	T292A	probably benign	Het
Trip11	G	A	12: 101,849,592 (GRCm39)	R92*	probably null	Het
Trpa1	G	A	1: 14,969,612 (GRCm39)	Q386*	probably null	Het
Trpc4	T	A	3: 54,187,410 (GRCm39)	V454D	probably damaging	Het
Ttc36	A	T	9: 44,714,051 (GRCm39)	D22E	probably benign	Het
Ttk	A	T	9: 83,751,316 (GRCm39)	I798F	probably damaging	Het
Ttn	T	C	2: 76,573,977 (GRCm39)	I17312V	probably damaging	Het
Txndc15	A	T	13: 55,865,875 (GRCm39)	E113V	possibly damaging	Het
Ube2d4	T	A	15: 58,718,448 (GRCm39)		noncoding transcript	Het
Urb1	T	A	16: 90,558,583 (GRCm39)	T1723S	probably benign	Het
Vmn1r65	A	T	7: 6,011,265 (GRCm39)	V323D	possibly damaging	Het
Vmn2r54	A	G	7: 12,366,238 (GRCm39)	M232T	probably benign	Het
Wdr59	A	G	8: 112,202,813 (GRCm39)	S577P	probably damaging	Het
Wfikkn2	C	T	11: 94,128,949 (GRCm39)	W397*	probably null	Het
Ypel1	T	A	16: 16,899,511 (GRCm39)		probably null	Het
Ythdf1	T	A	2: 180,552,763 (GRCm39)	D484V	probably damaging	Het
Zdhhc17	A	G	10: 110,783,154 (GRCm39)		probably null	Het
Zfp472	A	T	17: 33,184,887 (GRCm39)	H2L	possibly damaging	Het
Zfp953	A	T	13: 67,493,422 (GRCm39)	M74K	probably benign	Het
Zmat4	C	T	8: 24,419,151 (GRCm39)	T61M	probably benign	Het

Other mutations in Dnajc6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00162:Dnajc6	APN	4	101,365,286 (GRCm39)	intron	probably benign
IGL02336:Dnajc6	APN	4	101,471,483 (GRCm39)	splice site	probably null
IGL02551:Dnajc6	APN	4	101,496,550 (GRCm39)	missense	probably damaging	1.00
IGL02801:Dnajc6	APN	4	101,455,010 (GRCm39)	missense	probably benign	0.33
IGL02887:Dnajc6	APN	4	101,496,497 (GRCm39)	missense	probably damaging	1.00
IGL03107:Dnajc6	APN	4	101,474,057 (GRCm39)	missense	probably damaging	1.00
IGL03271:Dnajc6	APN	4	101,365,274 (GRCm39)	intron	probably benign
R0091:Dnajc6	UTSW	4	101,473,974 (GRCm39)	splice site	probably benign
R0384:Dnajc6	UTSW	4	101,456,153 (GRCm39)	missense	probably damaging	1.00
R0546:Dnajc6	UTSW	4	101,492,388 (GRCm39)	missense	probably damaging	0.99
R0689:Dnajc6	UTSW	4	101,468,450 (GRCm39)	missense	possibly damaging	0.91
R1239:Dnajc6	UTSW	4	101,492,313 (GRCm39)	missense	probably damaging	0.98
R1421:Dnajc6	UTSW	4	101,468,513 (GRCm39)	missense	probably damaging	0.97
R1424:Dnajc6	UTSW	4	101,496,544 (GRCm39)	missense	possibly damaging	0.92
R1563:Dnajc6	UTSW	4	101,456,334 (GRCm39)	missense	probably damaging	1.00
R1608:Dnajc6	UTSW	4	101,456,364 (GRCm39)	missense	probably damaging	1.00
R1757:Dnajc6	UTSW	4	101,455,028 (GRCm39)	missense	probably damaging	1.00
R2032:Dnajc6	UTSW	4	101,471,435 (GRCm39)	missense	probably benign	0.39
R2518:Dnajc6	UTSW	4	101,470,127 (GRCm39)	missense	probably damaging	0.99
R4028:Dnajc6	UTSW	4	101,474,054 (GRCm39)	missense	probably damaging	1.00
R4088:Dnajc6	UTSW	4	101,496,593 (GRCm39)	missense	probably damaging	1.00
R4601:Dnajc6	UTSW	4	101,468,461 (GRCm39)	missense	probably damaging	1.00
R4602:Dnajc6	UTSW	4	101,468,461 (GRCm39)	missense	probably damaging	1.00
R4610:Dnajc6	UTSW	4	101,468,461 (GRCm39)	missense	probably damaging	1.00
R4755:Dnajc6	UTSW	4	101,407,996 (GRCm39)	missense	probably damaging	1.00
R4878:Dnajc6	UTSW	4	101,456,231 (GRCm39)	intron	probably benign
R4938:Dnajc6	UTSW	4	101,494,010 (GRCm39)	missense	probably damaging	1.00
R5373:Dnajc6	UTSW	4	101,472,824 (GRCm39)	missense	probably damaging	0.99
R5391:Dnajc6	UTSW	4	101,485,355 (GRCm39)	critical splice donor site	probably null
R5435:Dnajc6	UTSW	4	101,463,807 (GRCm39)	missense	probably damaging	0.99
R5760:Dnajc6	UTSW	4	101,475,839 (GRCm39)	missense	probably benign	0.39
R6044:Dnajc6	UTSW	4	101,473,774 (GRCm39)	missense	probably benign	0.22
R6086:Dnajc6	UTSW	4	101,455,004 (GRCm39)	missense	probably benign	0.45
R6460:Dnajc6	UTSW	4	101,472,795 (GRCm39)	missense	probably damaging	1.00
R6495:Dnajc6	UTSW	4	101,492,262 (GRCm39)	nonsense	probably null
R6956:Dnajc6	UTSW	4	101,471,470 (GRCm39)	missense	probably damaging	0.97
R7072:Dnajc6	UTSW	4	101,472,812 (GRCm39)	missense	probably damaging	1.00
R7155:Dnajc6	UTSW	4	101,470,142 (GRCm39)	missense	probably damaging	1.00
R7192:Dnajc6	UTSW	4	101,455,000 (GRCm39)	missense	probably benign	0.02
R7226:Dnajc6	UTSW	4	101,496,569 (GRCm39)	missense	probably damaging	1.00
R7298:Dnajc6	UTSW	4	101,463,808 (GRCm39)	missense	probably benign	0.09
R7612:Dnajc6	UTSW	4	101,455,123 (GRCm39)	missense	probably benign	0.40
R7622:Dnajc6	UTSW	4	101,497,688 (GRCm39)	missense	probably damaging	1.00
R7652:Dnajc6	UTSW	4	101,463,874 (GRCm39)	missense	probably damaging	0.98
R7789:Dnajc6	UTSW	4	101,475,729 (GRCm39)	missense	possibly damaging	0.82
R8010:Dnajc6	UTSW	4	101,475,611 (GRCm39)	missense	probably benign	0.01
R8201:Dnajc6	UTSW	4	101,475,960 (GRCm39)	missense	probably benign	0.08
R8305:Dnajc6	UTSW	4	101,480,984 (GRCm39)	missense	probably damaging	1.00
R8912:Dnajc6	UTSW	4	101,468,513 (GRCm39)	missense	probably damaging	1.00
R9012:Dnajc6	UTSW	4	101,470,156 (GRCm39)	missense	probably damaging	1.00
R9052:Dnajc6	UTSW	4	101,496,617 (GRCm39)	missense	probably damaging	0.97
R9091:Dnajc6	UTSW	4	101,496,559 (GRCm39)	missense	possibly damaging	0.80
R9160:Dnajc6	UTSW	4	101,470,258 (GRCm39)	unclassified	probably benign
R9258:Dnajc6	UTSW	4	101,475,813 (GRCm39)	missense	probably benign	0.07
R9270:Dnajc6	UTSW	4	101,496,559 (GRCm39)	missense	possibly damaging	0.80
R9294:Dnajc6	UTSW	4	101,408,054 (GRCm39)	critical splice donor site	probably null
R9386:Dnajc6	UTSW	4	101,494,098 (GRCm39)	critical splice donor site	probably null
R9664:Dnajc6	UTSW	4	101,475,821 (GRCm39)	missense	probably benign	0.04
Z1088:Dnajc6	UTSW	4	101,496,526 (GRCm39)	missense	probably damaging	1.00
Z1177:Dnajc6	UTSW	4	101,496,625 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- TGCCTTCCCTTAGTGATTAGAGG -3'
(R):5'- TTCGGTAAGACTTGGGTGAC -3'

Sequencing Primer
(F):5'- CTTCCCTTAGTGATTAGAGGAAAGAG -3'
(R):5'- TGTCTGGAATCCAAAAAGCTACG -3'

Posted On

2014-06-23