Mutagenetix > Incidental Mutation

Incidental Mutation 'R1856:Lama3'

206185

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

039880-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1856 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

12466876-12716070 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 12670838 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 808 (L808*)

Ref Sequence

ENSEMBL: ENSMUSP00000140104 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070] [ENSMUST00000188815]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000092070
AA Change: L2414*

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: L2414*

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000188815
AA Change: L808*

SMART Domains

Protein: ENSMUSP00000140104
Gene: ENSMUSG00000024421
AA Change: L808*

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_Lam	78	122	2.66e-10	SMART
EGF_Lam	125	175	7.81e-8	SMART
Pfam:Laminin_I	230	496	1e-90	PFAM
low complexity region	579	594	N/A	INTRINSIC
coiled coil region	605	632	N/A	INTRINSIC
LamG	800	960	1.67e-2	SMART
LamG	1008	1136	1.72e-17	SMART
LamG	1179	1294	3.96e-17	SMART
LamG	1399	1527	1.12e-34	SMART
LamG	1569	1702	3.41e-30	SMART

Coding Region Coverage

1x: 97.4%
3x: 96.8%
10x: 95.1%
20x: 91.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm5	T	A	4: 144,504,453 (GRCm39)	M233L	probably benign	Het
Abca14	T	G	7: 119,877,404 (GRCm39)	F1017L	probably damaging	Het
Abcb11	A	C	2: 69,076,267 (GRCm39)	V1147G	probably damaging	Het
Abcc10	T	A	17: 46,617,529 (GRCm39)	S1128C	probably damaging	Het
Adam6a	G	A	12: 113,508,923 (GRCm39)	C432Y	probably damaging	Het
Adamdec1	C	T	14: 68,808,397 (GRCm39)	V318M	probably damaging	Het
Ahnak	C	A	19: 8,979,412 (GRCm39)	S232Y	possibly damaging	Het
Amt	A	C	9: 108,174,361 (GRCm39)	H42P	probably damaging	Het
Anapc1	A	T	2: 128,501,708 (GRCm39)	L778Q	probably damaging	Het
Ankhd1	G	A	18: 36,777,580 (GRCm39)	A1588T	probably benign	Het
Anln	A	T	9: 22,264,627 (GRCm39)	L881Q	probably damaging	Het
Appl1	A	G	14: 26,649,706 (GRCm39)	S607P	probably damaging	Het
Arg2	G	T	12: 79,194,436 (GRCm39)	V87L	probably benign	Het
Atp13a2	C	T	4: 140,731,323 (GRCm39)	P869L	probably benign	Het
Atxn7l1	T	A	12: 33,408,769 (GRCm39)	D310E	probably damaging	Het
Cd6	A	T	19: 10,775,966 (GRCm39)	D164E	probably damaging	Het
Cdan1	C	A	2: 120,555,417 (GRCm39)	V775L	probably benign	Het
Cep164	A	T	9: 45,687,056 (GRCm39)		probably null	Het
Cep41	A	G	6: 30,661,005 (GRCm39)	S61P	probably damaging	Het
Clcn7	C	A	17: 25,379,445 (GRCm39)	D764E	probably damaging	Het
Cog2	G	A	8: 125,278,142 (GRCm39)	G703S	possibly damaging	Het
Cramp1	T	C	17: 25,187,952 (GRCm39)	D1214G	probably damaging	Het
Cst7	G	T	2: 150,419,628 (GRCm39)	C98F	probably damaging	Het
Ctc1	T	A	11: 68,925,484 (GRCm39)	L1007Q	probably damaging	Het
Cyb5r4	G	A	9: 86,904,262 (GRCm39)	A11T	possibly damaging	Het
Dcaf8	A	G	1: 172,003,120 (GRCm39)	D306G	probably damaging	Het
Defb38	T	A	8: 19,073,592 (GRCm39)	K27I	probably benign	Het
Disp3	T	C	4: 148,356,089 (GRCm39)	E257G	probably damaging	Het
Dnajc6	T	C	4: 101,456,185 (GRCm39)	S58P	probably damaging	Het
Dock10	A	T	1: 80,584,285 (GRCm39)	D140E	possibly damaging	Het
Dop1a	T	C	9: 86,374,057 (GRCm39)	V172A	probably damaging	Het
Dync1h1	G	A	12: 110,602,943 (GRCm39)	E2195K	probably benign	Het
Eci3	C	T	13: 35,137,011 (GRCm39)	A171T	possibly damaging	Het
Eml2	A	G	7: 18,927,986 (GRCm39)	D284G	probably damaging	Het
Eml5	T	C	12: 98,776,843 (GRCm39)	D1377G	probably damaging	Het
Fam186a	T	C	15: 99,838,183 (GRCm39)	E2687G	possibly damaging	Het
Fut9	A	C	4: 25,620,352 (GRCm39)	L154R	probably damaging	Het
Gabrb2	A	G	11: 42,517,540 (GRCm39)	N454S	probably benign	Het
Gpr183	T	A	14: 122,192,153 (GRCm39)	I123L	probably benign	Het
Gucy1b1	A	T	3: 81,965,659 (GRCm39)	N62K	probably benign	Het
Hcrtr2	T	C	9: 76,167,067 (GRCm39)	N90S	probably damaging	Het
Hectd1	A	T	12: 51,791,577 (GRCm39)	L2556Q	probably damaging	Het
Hfm1	T	A	5: 106,995,542 (GRCm39)	M1290L	probably benign	Het
Hgsnat	A	G	8: 26,447,284 (GRCm39)	W337R	probably benign	Het
Hmbox1	T	C	14: 65,066,097 (GRCm39)	Y291C	probably damaging	Het
Hmcn1	T	C	1: 150,597,415 (GRCm39)	N1749S	probably benign	Het
Igsf10	T	C	3: 59,238,693 (GRCm39)	D496G	possibly damaging	Het
Iqch	C	T	9: 63,441,619 (GRCm39)		probably null	Het
Irf6	A	G	1: 192,849,843 (GRCm39)	D255G	probably benign	Het
Kif9	G	A	9: 110,346,787 (GRCm39)	G642R	probably null	Het
Klhl20	A	G	1: 160,934,312 (GRCm39)	Y236H	probably benign	Het
Krt39	T	A	11: 99,409,914 (GRCm39)	K208*	probably null	Het
Lrp5	C	T	19: 3,647,346 (GRCm39)	A1299T	probably benign	Het
Lysmd4	A	G	7: 66,875,979 (GRCm39)	Q214R	probably benign	Het
Macf1	T	A	4: 123,263,641 (GRCm39)	E6960V	probably damaging	Het
Mcpt2	A	G	14: 56,281,156 (GRCm39)	E120G	probably benign	Het
Mpeg1	A	G	19: 12,439,720 (GRCm39)	T393A	probably benign	Het
Myh4	G	A	11: 67,146,508 (GRCm39)	E1494K	probably damaging	Het
Nbas	T	G	12: 13,524,230 (GRCm39)	S1695R	possibly damaging	Het
Nlgn1	G	T	3: 25,494,201 (GRCm39)	Y249*	probably null	Het
Ntn4	C	T	10: 93,543,215 (GRCm39)	R314W	probably damaging	Het
Nup107	A	G	10: 117,586,811 (GRCm39)	L910P	probably damaging	Het
Obscn	T	A	11: 58,931,122 (GRCm39)	D5838V	probably damaging	Het
Or10g7	T	C	9: 39,905,655 (GRCm39)	I183T	probably benign	Het
Or1j18	T	C	2: 36,624,357 (GRCm39)	I8T	probably benign	Het
Or4c113	G	A	2: 88,885,203 (GRCm39)	T189M	possibly damaging	Het
Or8d2b	T	C	9: 38,788,892 (GRCm39)	I140T	possibly damaging	Het
Otogl	G	T	10: 107,690,125 (GRCm39)	S915Y	possibly damaging	Het
P2rx7	T	A	5: 122,819,095 (GRCm39)	C506S	probably damaging	Het
P4hb	T	C	11: 120,454,044 (GRCm39)	E350G	probably benign	Het
Papolg	A	T	11: 23,817,379 (GRCm39)	V606E	probably benign	Het
Pappa	A	T	4: 65,258,980 (GRCm39)	D1576V	probably damaging	Het
Pclo	G	T	5: 14,828,566 (GRCm39)	V1402F	probably damaging	Het
Pdcd11	A	G	19: 47,086,626 (GRCm39)	T211A	probably benign	Het
Pdzd2	T	C	15: 12,373,941 (GRCm39)	R2065G	possibly damaging	Het
Plcl2	T	C	17: 50,914,878 (GRCm39)	V629A	probably benign	Het
Prkcsh	G	A	9: 21,915,871 (GRCm39)	V92I	possibly damaging	Het
Prpf19	G	T	19: 10,879,780 (GRCm39)	V320F	probably damaging	Het
Ptpn3	T	A	4: 57,239,682 (GRCm39)	I283F	probably damaging	Het
Rcc2	T	A	4: 140,447,915 (GRCm39)	D480E	probably benign	Het
Rnf182	G	A	13: 43,821,518 (GRCm39)	C23Y	probably damaging	Het
Rnf186	C	T	4: 138,694,673 (GRCm39)	T71I	probably benign	Het
Scyl3	A	G	1: 163,761,265 (GRCm39)		probably null	Het
Slc12a6	G	T	2: 112,166,272 (GRCm39)		probably null	Het
Slc1a2	C	A	2: 102,607,912 (GRCm39)	S520Y	probably damaging	Het
Slc35e2	T	A	4: 155,696,186 (GRCm39)	L191Q	probably damaging	Het
Snrnp40	C	G	4: 130,271,836 (GRCm39)		probably null	Het
Snx33	T	C	9: 56,833,295 (GRCm39)	H258R	possibly damaging	Het
Tecpr2	A	T	12: 110,899,498 (GRCm39)	H622L	probably benign	Het
Thbs3	A	T	3: 89,133,713 (GRCm39)	E888D	probably damaging	Het
Tlk2	T	C	11: 105,112,124 (GRCm39)	L159P	probably benign	Het
Tmem70	A	T	1: 16,747,497 (GRCm39)	T205S	probably damaging	Het
Trappc10	T	A	10: 78,032,285 (GRCm39)	H1001L	probably benign	Het
Trim12a	T	C	7: 103,950,064 (GRCm39)	T292A	probably benign	Het
Trip11	G	A	12: 101,849,592 (GRCm39)	R92*	probably null	Het
Trpa1	G	A	1: 14,969,612 (GRCm39)	Q386*	probably null	Het
Trpc4	T	A	3: 54,187,410 (GRCm39)	V454D	probably damaging	Het
Ttc36	A	T	9: 44,714,051 (GRCm39)	D22E	probably benign	Het
Ttk	A	T	9: 83,751,316 (GRCm39)	I798F	probably damaging	Het
Ttn	T	C	2: 76,573,977 (GRCm39)	I17312V	probably damaging	Het
Txndc15	A	T	13: 55,865,875 (GRCm39)	E113V	possibly damaging	Het
Ube2d4	T	A	15: 58,718,448 (GRCm39)		noncoding transcript	Het
Urb1	T	A	16: 90,558,583 (GRCm39)	T1723S	probably benign	Het
Vmn1r65	A	T	7: 6,011,265 (GRCm39)	V323D	possibly damaging	Het
Vmn2r54	A	G	7: 12,366,238 (GRCm39)	M232T	probably benign	Het
Wdr59	A	G	8: 112,202,813 (GRCm39)	S577P	probably damaging	Het
Wfikkn2	C	T	11: 94,128,949 (GRCm39)	W397*	probably null	Het
Ypel1	T	A	16: 16,899,511 (GRCm39)		probably null	Het
Ythdf1	T	A	2: 180,552,763 (GRCm39)	D484V	probably damaging	Het
Zdhhc17	A	G	10: 110,783,154 (GRCm39)		probably null	Het
Zfp472	A	T	17: 33,184,887 (GRCm39)	H2L	possibly damaging	Het
Zfp953	A	T	13: 67,493,422 (GRCm39)	M74K	probably benign	Het
Zmat4	C	T	8: 24,419,151 (GRCm39)	T61M	probably benign	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,713,349 (GRCm39)	missense	probably benign
IGL00272:Lama3	APN	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,582,645 (GRCm39)	splice site	probably benign
IGL00836:Lama3	APN	18	12,605,285 (GRCm39)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,574,200 (GRCm39)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,614,094 (GRCm39)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,664,983 (GRCm39)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,574,188 (GRCm39)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,586,937 (GRCm39)	splice site	probably benign
IGL01863:Lama3	APN	18	12,552,993 (GRCm39)	splice site	probably benign
IGL01869:Lama3	APN	18	12,657,820 (GRCm39)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,705,121 (GRCm39)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,649,570 (GRCm39)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,601,371 (GRCm39)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,714,840 (GRCm39)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,624,533 (GRCm39)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,689,807 (GRCm39)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,690,784 (GRCm39)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,670,915 (GRCm39)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,658,910 (GRCm39)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,711,184 (GRCm39)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,661,858 (GRCm39)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,601,425 (GRCm39)	splice site	probably benign
IGL03038:Lama3	APN	18	12,552,307 (GRCm39)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,572,406 (GRCm39)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,660,681 (GRCm39)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,614,095 (GRCm39)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,672,760 (GRCm39)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,686,340 (GRCm39)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,552,239 (GRCm39)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,686,288 (GRCm39)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,673,024 (GRCm39)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0106:Lama3	UTSW	18	12,537,039 (GRCm39)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,581,329 (GRCm39)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,657,867 (GRCm39)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0316:Lama3	UTSW	18	12,652,934 (GRCm39)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,615,183 (GRCm39)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,640,064 (GRCm39)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,540,620 (GRCm39)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,589,894 (GRCm39)	missense	probably benign
R0449:Lama3	UTSW	18	12,633,569 (GRCm39)	splice site	probably null
R0453:Lama3	UTSW	18	12,598,535 (GRCm39)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,583,481 (GRCm39)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,658,951 (GRCm39)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,694,758 (GRCm39)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,682,309 (GRCm39)	missense	probably benign
R0605:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,552,315 (GRCm39)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,552,302 (GRCm39)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,610,647 (GRCm39)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,589,907 (GRCm39)	splice site	probably benign
R1216:Lama3	UTSW	18	12,554,191 (GRCm39)	splice site	probably benign
R1356:Lama3	UTSW	18	12,633,634 (GRCm39)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,610,427 (GRCm39)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,653,048 (GRCm39)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,614,155 (GRCm39)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,682,284 (GRCm39)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,615,102 (GRCm39)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,646,788 (GRCm39)	splice site	probably benign
R1571:Lama3	UTSW	18	12,672,774 (GRCm39)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,583,457 (GRCm39)	nonsense	probably null
R1631:Lama3	UTSW	18	12,540,551 (GRCm39)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,612,929 (GRCm39)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,598,556 (GRCm39)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,535,119 (GRCm39)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,646,762 (GRCm39)	missense	possibly damaging	0.75
R1909:Lama3	UTSW	18	12,714,855 (GRCm39)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,628,336 (GRCm39)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,586,920 (GRCm39)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,657,778 (GRCm39)	splice site	probably benign
R2059:Lama3	UTSW	18	12,661,390 (GRCm39)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,661,783 (GRCm39)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,657,887 (GRCm39)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,535,906 (GRCm39)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,658,136 (GRCm39)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,546,915 (GRCm39)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,581,345 (GRCm39)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,572,532 (GRCm39)	splice site	probably benign
R3752:Lama3	UTSW	18	12,640,086 (GRCm39)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,637,365 (GRCm39)	nonsense	probably null
R4118:Lama3	UTSW	18	12,583,488 (GRCm39)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,646,747 (GRCm39)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,715,588 (GRCm39)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,652,929 (GRCm39)	nonsense	probably null
R4483:Lama3	UTSW	18	12,682,310 (GRCm39)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,614,145 (GRCm39)	missense	probably benign
R4516:Lama3	UTSW	18	12,628,415 (GRCm39)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,612,816 (GRCm39)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,637,454 (GRCm39)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,711,086 (GRCm39)	nonsense	probably null
R4704:Lama3	UTSW	18	12,686,280 (GRCm39)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,637,416 (GRCm39)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,615,141 (GRCm39)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,633,620 (GRCm39)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,546,828 (GRCm39)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,544,627 (GRCm39)	nonsense	probably null
R4784:Lama3	UTSW	18	12,582,601 (GRCm39)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,610,661 (GRCm39)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,574,188 (GRCm39)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,544,599 (GRCm39)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,631,735 (GRCm39)	intron	probably benign
R4863:Lama3	UTSW	18	12,672,850 (GRCm39)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,581,362 (GRCm39)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,685,883 (GRCm39)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,651,800 (GRCm39)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,715,668 (GRCm39)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,665,005 (GRCm39)	missense	probably null	0.82
R5090:Lama3	UTSW	18	12,675,459 (GRCm39)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,672,823 (GRCm39)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,710,957 (GRCm39)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,552,950 (GRCm39)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,598,565 (GRCm39)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,685,912 (GRCm39)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,586,803 (GRCm39)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,705,123 (GRCm39)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,589,821 (GRCm39)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,686,267 (GRCm39)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,605,277 (GRCm39)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,572,405 (GRCm39)	missense	probably benign
R5617:Lama3	UTSW	18	12,631,993 (GRCm39)	intron	probably benign
R5709:Lama3	UTSW	18	12,672,856 (GRCm39)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,562,944 (GRCm39)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,707,311 (GRCm39)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,602,985 (GRCm39)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,615,156 (GRCm39)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,646,702 (GRCm39)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,657,794 (GRCm39)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,615,194 (GRCm39)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,612,813 (GRCm39)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,615,205 (GRCm39)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,628,405 (GRCm39)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,670,823 (GRCm39)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,710,897 (GRCm39)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,552,314 (GRCm39)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,646,735 (GRCm39)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,682,283 (GRCm39)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,661,475 (GRCm39)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,649,605 (GRCm39)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,715,602 (GRCm39)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,715,701 (GRCm39)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,685,870 (GRCm39)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,664,936 (GRCm39)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,595,839 (GRCm39)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,672,843 (GRCm39)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,601,346 (GRCm39)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,589,869 (GRCm39)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,537,133 (GRCm39)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,563,057 (GRCm39)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,715,665 (GRCm39)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,552,902 (GRCm39)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,572,449 (GRCm39)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,640,097 (GRCm39)	splice site	probably null
R7442:Lama3	UTSW	18	12,605,238 (GRCm39)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,552,294 (GRCm39)	missense	probably benign
R7604:Lama3	UTSW	18	12,633,550 (GRCm39)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,664,891 (GRCm39)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,670,895 (GRCm39)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,595,864 (GRCm39)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,670,796 (GRCm39)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,667,120 (GRCm39)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,639,999 (GRCm39)	missense	probably null
R8219:Lama3	UTSW	18	12,572,417 (GRCm39)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8229:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8298:Lama3	UTSW	18	12,658,910 (GRCm39)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,673,670 (GRCm39)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,661,404 (GRCm39)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,582,896 (GRCm39)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,544,688 (GRCm39)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,554,212 (GRCm39)	missense	probably benign
R8799:Lama3	UTSW	18	12,624,000 (GRCm39)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,582,643 (GRCm39)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,689,762 (GRCm39)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,665,096 (GRCm39)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,614,120 (GRCm39)	nonsense	probably null
R9126:Lama3	UTSW	18	12,583,527 (GRCm39)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,605,297 (GRCm39)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,595,869 (GRCm39)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,710,959 (GRCm39)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,583,541 (GRCm39)	nonsense	probably null
R9553:Lama3	UTSW	18	12,563,019 (GRCm39)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,682,320 (GRCm39)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,715,631 (GRCm39)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,562,936 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- AACTCAGACAAGCCACTGTG -3'
(R):5'- ACCTCCATAGCACAGAAGGG -3'

Sequencing Primer
(F):5'- CAAGCCACTGTGTTGCTAAG -3'
(R):5'- CTGTCTTTGTAGGACAAGCAAATG -3'

Posted On

2014-06-23